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Do, Su-Il,Kim, Kyung-Sook,Kim, Hee-Nam,Kim, Yeon-Jeong,Lee, Ki-Young,Kim, Bang-Ul,Kim, Cheorl-Ho,Lee, Young-Choon 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
The cDNA encoding human Sia-α2,3-Gal-β1,4-Glc-NAc-R:α2,8-sialyltransferase, hST8Sia III, was isolated by screening of a human brain cDNA library with polymerase chain reaction-amplified DNA probe generated from the sequence of mouse ST8Sia III (mST8Sia III) and by 5' rapid amplification of cDNA ends of mRNA isolated from human brain tissues. Comparative analysis of the predicted protein-coding region between our cloned hST8Sia III and mST8Sia III showed 92 and 96% identities in the nucleotide and the amino acid sequence, respectively. The soluble hST8Sia III protein expressed in COS-7 showed an extremely high catalytic activity of transferring sialic acid through α2,8-linkage to intact fetuin glycoprotein, whereas the transferring activity was completely undetectable toward either α2,6-sialylated glycoprotein or desialylated glycoprotein acceptors. Northern analysis of hST8Sia III showed that the transcript corresponding to 11 kb was expressed in both human fetal and adult brain, while the expression of the 5.5-kb transcript was restricted to fetal liver, indicating that the expression of hST8Sia III is developmentally and tissue-specifically regulated.
Moya moya disease 환자(患者) 2례(例)에 대한 임상적(臨床的) 고찰(考察)
김행진,이경환,김용진,김방울,전상윤,홍석,노영만,Kim Haeng-Jin,Lee Kyoung-Hwan,Kim Yong-Jin,Kim Bang-Ul,Jun Sang-Yun,Hong Seok,No Young-Man 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.4
Objectives: This study was designed to analyze the effectiveness of the diagnosis and treatments of Moya moya disease in oriental medicine. Results: This study showed that clinical symptoms of two Moya moya patients were improved by the diagnosis and treatments of oriental medicine. Conclusions: Diagnosis and treatment of two patients with Moya moya disease are accurate and effective. However, more cases are required to study in oriental medicine in order to prove the availability and to be applied universally.