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Single-Port Robotic Cholecystectomy: Early Experience from 8 Cases
( Hyung Jun Kwon ),( Horyon Kong ),( Sang Geol Kim ),( Yun Jin Hwang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Single-port laparoscopic cholecystectomy (SPLC) is a technical concept to reduced pain and improve cosmetic results when compared to multi-port cholecystectomy. However, SPLC is associated with technical limitation due to the enhanced complexity of the approach and limited number of specialized instruments or platforms. On the other hand, using a robotic platform may overcome these problems and enable more precise surgical actions by increasing freedom of movement and by restoring intuitive instrument control. In this presentation, we report the early clinical experience of our first 8 sing-port robotic cholecystectomy (SPRC) cases. Methods: Between November 2016 and February 2017, eight patients underwent SPRC with the da Vinci Xi robot and single-site instrumentation. We retrospectively reviewed clinical data on those patients. Results: All of 8 patients had completion of SPRC. Seven patients were female and one was male. Average patient age was 43.3±11.8 years and BMI was 22.4±1.4 kg/m2. Three patients (37.5%) were diagnosed with chronic calculous cholecystitis. Three patients (37.5%) underwent operation for polypoid lesions of the gallbladder. One patient (12.5%) was diagnosed with acute calculous cholecystitis. The mean operation time (skin-to-skin) was 83.9±30.7 min, docking time was 13.25±8.1 min, and console time was 42.1±26.4 min. The intraoperative blood loss was negligible. The mean Visual Analog Pain Scale score 6hr after the surgery was 2.9±0.4. The mean length of hospital stay average postoperative hospital stay was 2.3±1.0 day. There were no intraoperative complication and one patient developed seroma on port site. Conclusions: Robotic single-port cholecystectomy appears feasible and safe in our early experience.
Jun, Soo Youn,Jung, Gi Mo,Yoon, Seong Jun,Choi, Yun-Jaie,Koh, Woo Suk,Moon, Kyoung Sik,Kang, Sang Hyeon American Society for Microbiology 2014 Antimicrobial Agents and Chemotherapy Vol.58 No.4
<P>Phage endolysins have received increasing attention as potent antibacterial agents. However, although safety evaluation is a prerequisite for the drug development process, a good laboratory practice (GLP)-compliant safety evaluation has not been reported for phage endolysins. A safety evaluation of intravenously administered SAL200 (containing phage endolysin SAL-1) was conducted according to GLP standards. No animals died in any of the safety evaluation studies. In general toxicity studies, intravenously administered SAL200 showed no sign of toxicity in rodent single- and repeated-dose toxicity studies. In the dog repeated-dose toxicity test, there were no abnormal findings, with the exception of transient abnormal clinical signs that were observed in some dogs when daily injection of SAL200 was continued for more than 1 week. In safety pharmacology studies, there were also no signs of toxicity in the central nervous and respiratory system function tests. In the cardiovascular function test, there were no abnormal findings in all tested dogs after the first and second administrations, but transient abnormalities were observed after the third and fourth administrations (2 or 3 weeks after the initial administration). All abnormal findings observed in these safety evaluation studies were slight to mild, were apparent only transiently after injection, and resolved quickly. The safety evaluation results for SAL200 support the implementation of an exploratory phase I clinical trial and underscore the potential of SAL200 as a new drug. We have designed an appropriate phase I clinical trial based on the results of this study.</P>
( Sung Hoon Choi ),( Hye Won Shin ),( Jun Yong Park ),( Ji Young Yoo ),( Do Young Kim ),( Weon Sang Ro ),( Chae Ok Yun ),( Kwang Hyub Han ) 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.3
Background/Aims: Hypoxia-inducible factor-1α (HIF-1α) is a central transcriptional factor involved in the cellular responses related to various aspects of cancer biology, including proliferation, survival, and angiogenesis, and the metabolism of the extracellular matrix in hypoxia. This study evaluated whether adenovirus-mediated small hairpin RNA (shRNA) against HIF-1α (shHIF-1α) inhibits cell proliferation and angiogenesis in hepatocellular carcinoma (HCC) cell lines. Methods: Knockdown of HIF-1α expression was constructed by adenovirus-mediated RNA interference tools, and HCC cell lines infected with shHIF-1α coding virus were cultured under a hypoxia condition (1% O2) for 24 hours. Following infection, the expression levels of HIF-1α, angiogenesis factors, and matrix metalloproteinase (MMP) were examined using Western blotting. Cell proliferation and angiogenesis were measured by a cell proliferation assay (MTT assay) and an angiogenesis-related assay (invasion and tube-formation assay), respectively. Results: Adenovirus mediated inhibition of HIF-1α induced suppression of tumor growth in HCC cell lines. It also down-regulated the expression of angiogenesis factor and MMP proteins. Angiogenesis as well as mobility of vascular cells to tumor was suppressed by adenovirus-mediated shHIF-1α-infected groups in human umbilical vein endothelial cells (HUVECs). Conclusions: These data suggest that adenovirus-mediated inhibition of HIF-1α inhibits the invasion, tube formation, and cell growth in HUVECs and HCC cells. (Korean J Hepatol 2010;16:280-287)
Single-Electron-Based Flexible Multivalued Exclusive-<small>or</small> Logic Gate
Sang-Jin Kim,Chang-Keun Lee,Rae-Sik Chung,Eun-Sil Park,Seung-Jun Shin,Jung-Bum Choi,Yun-Seop Yu,Nam-Soo Kim,Hyung Gyoo Lee,Keun-Hyung Park IEEE 2009 IEEE transactions on electron devices Vol.56 No.5
<P>By using two symmetrical sidewall gates, we implemented a Si-based single-electron exclusive- OR (XOR) gate and reported on the first flexible multivalued (MV) functionality. A grayscale contour plot of the output voltages displays alternating high/low values as a function of two single-electron transistor (SET) input voltages. Their voltage transfer characteristics display typical XOR or XNOR gate function depending on input voltages for binary, MV, and binary-MV mixed-modes. This flexible two-input XOR gate, combined with the previously reported NAND/NOR gates, provide three basic arithmetic blocks for the SET-based MV logic gate family.</P>
Cellulitis presenting as necrotizing fasciitis
( Sang Youl Yun ),( Jun Gyu Song ),( Moo Kyu Suh ),( Jong Im Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1
Cellulitis is an acute infection of the dermis and subcutaneous fat, that presents with erythema, pain, firm and tender induration, and less commonly, fluctuance. In some cases of cellulitis, the overlying epidermis undergoes bulla formation or necrosis, resulting in extensive areas of epidermal sloughing and superficial erosion. Necrotizing fasciitis is an uncommon infection of the subcutaneous soft tissue and fascia. Clinical diagnosis is often initially confused with cellulitis, and delay in diagnosis and treatment is highly lethal, so an early diagnosis and a radical debridement of all affected tissues is necessary. We report a case of cellulitis presenting as necrotizing fasciitis in a 56-year-old man. He presented with localized painful erythematous patches, hemorrhagic bullae with swelling on the left lower leg for 3 days. Laboratory tests revealed the elevated levels of WBC counts(16, 580/mm3), hepatic enzymes (AST 121IU, ALT 176IU), and CRP(15.74). He has high fever, but blood culture was negative. Skin biopsy specimen showed moderate infiltrates of mixed inflammatory cells through the dermis. MRI showed diffuse, ill-defined, irregular linear and reticular high signal intensity soft tissue change with swelling at subcutaneous fat layer of lower leg. He was treated with systemic antibiotics(cefotaxime 3g, ciprofloxacin 800mg/day). The skin lesions improved one month after treatment, and recurrence has not been observed.
Yun, Jieun,Han, Sang-Bae,Kim, Hong Jun,Go, Se-il,Lee, Won Sup,Bae, Woo Kyun,Cho, Sang-Hee,Song, Eun-Kee,Lee, Ok-Jun,Kim, Hee Kyung,Yang, Yaewon,Kwon, Jihyun,Chae, Hee Bok,Lee, Ki Hyeong,Han, Hye Sook The Korean Gastric Cancer Association 2019 Journal of gastric cancer Vol.19 No.3
Purpose: Peritoneal carcinomatosis in gastric cancer (GC) patients results in extremely poor prognosis. Malignant ascites samples are the most appropriate biological material to use to evaluate biomarkers for peritoneal carcinomatosis. This study identified exosomal MicroRNAs (miRNAs) differently expressed between benign liver cirrhosis-associated ascites (LC-ascites) and malignant gastric cancer-associated ascites (GC-ascites), and validated their role as diagnostic biomarkers for GC-ascites. Materials and Methods: Total RNA was extracted from exosomes isolated from 165 ascites samples (73 LC-ascites and 92 GC-ascites). Initially, microarrays were used to screen the expression levels of 2,006 miRNAs in the discovery cohort (n=22). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analyses were performed to validate the expression levels of selected exosomal miRNAs in the training (n=70) and validation (n=73) cohorts. Furthermore, carcinoembryonic antigen (CEA) levels were determined in ascites samples. Results: The miR-574-3p, miR-181b-5p, miR-4481, and miR-181d were significantly downregulated in the GC-ascites samples compared to the LC-ascites samples, and miR-181b-5p showed the best diagnostic performance for GC-ascites (area under the curve [AUC]=0.798 and 0.846 for the training and validation cohorts, respectively). The diagnostic performance of CEA for GC-ascites was improved by the combined analysis of miR-181b-5p and CEA (AUC=0.981 and 0.946 for the training and validation cohorts, respectively). Conclusions: We identified exosomal miRNAs capable of distinguishing between non-malignant and GC-ascites, showing that the combined use of miR-181b-5p and CEA could improve diagnosis.