착화제와 유기산이 Wistar rat체내의 Sr 분포에 미치는 영향

이기호,이제호,박상윤,이승훈,유용운,윤택구 대한방사선 방어학회 1990 방사선방어학회지 Vol.15 No.2

Wistar rat에 85SrCl2를 꼬리 정맥에 주사하여 체내 기관과 혈액 내 분포, 잔존율을 조사하였고 착화제와 유기산을 투여하여 혈장 단백질에 결합하는 Sr양의 변화를 측정하였다. 혈액내에서 Sr은 혈장에 60%, 세포에 40%부착되어 이동하였다. 혈장에 존재하는 Sr중 약 50%정도는 혈장 단백질과 결합한 상태였고, 세표에는 세포 표면에 가볍게 부착되어 있었다. Erythrocyte나 granulocyte보다 lymphocyte에 많은 양의 Sr이 부착되어 있었다. 투여후 초기 1시간 이내에 혈액 내에서 급격히 감소하여 뼈에 침착되었다. 이때 각 기관에서도 Sr의 잔존율은 24시간 이내에 크게 감소하였고, 뼈로 침착된 Sr은 24시간 이후에 서서히 감소하였다. 착화제 EDTA, EGTA 및 DTPA를 투여한 경우, 혈장 단백질에 결합하는 Sr의 양은 대조군의 57%에서 27-33%로 감소하였으며 citrate 및 oxalate의 투여시는 이값이 19%와 40%로 각각 감소하였다. 85SrCl2 was injected to the tail vein of Wistar rats and investigated its distribution and clearance in the tissues and blood. We also measured the changes in Sr binding to the blood plasma protein by administrating chelating agents and organic acids. For the blood, 60% of the Sr occurred in the plasma and 40% on the cell membrane. Fifty percent of Sr in the blood plasma was bound to plasma protein. Sr on the cell membrane seemed to be bound loosely. The binding in the lymphocyte was higher than in the erythrocyte and granulocyte. Within one hour Sr was quickly disappeared from the blood stream, to be accumulated in the bone. Twenty four hours after the injection, Sr decreased rapidly in the organs of soft tissue, but slowly in the bone. The binding of Sr to plasma protien decreased from 57% of the control to 27-33% in the group treated with chelating agents. EDTA. EGTA and DTPA and to 19% and 40% in the groups treated with organic acids, citrate and oxalate, respectively.

복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

HIV 감염 환자에서 발생한 편평 콘딜롬 1예

이해웅,오상현,장성은,최지호,문기찬,고재경 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4

사립체 DNA 뉴클레오타이드 3243 A→G 돌연변이 [MTTL1^*MELAS3243G]에 의한 MELAS 증후군

박기형,김승현,이영주,김희태,김주한,김명호,박성섭 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

L_iF 紛末의 Exo-emission Parameter

李春鎬,李起芳 全北大學校 基礎科學硏究所 1978 基礎科學 Vol.1 No.1

We make use of a method for calculating the thermoluminescence(or T.L) parameters to deduce the activation energy and the frequency factor of Lif crystal powder from TSEE glow curve on the assumption that the second-order kinetics of TL is closely related to the exoemision of trapped electron. The activation energy, also, has been calculated by the method of Kelly and Laubitz for the calculation of the activation energy from two points of the glow peaks. The deduced values of the activation evergy and the frequency factor of LiF pawder at peak temperatures 120℃ and 225℃ are 1.34±0.12ev, 1.65±0.01ev, and 4.2×10 exp (-18) sec and 6.3×10 exp (-16). According to the result of the study, we may conclude that the exo-emission of LiF powder follows the second-order kinetics of thermoluminescence.

HPV에 감염된 자궁경부 상피세포의 Keratin 아형 변화에 관한 연구

기근홍,이영미,장원재,임용,임성철,김창원,전호종 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The human papillomavirus (HPV) are wide spread in the world, causing proliferation of epidermal and mucosal surface. Certain papillomaviral types are oncogenic in vivo and in vitro. HPV DNA has been detected in most of cervical lesions such as condyloma acuminata, dysplasias, and invasive carcinomas. The materials for this study consisted of 5 dysplasias. 5 squamous cell carcinomas, and 5 exocervix for control, that resected for definite treatment of uterine disease at Chosen University Hospital from January, 1991 to December, 1993. All cases were performed of in situ hybridization for HPV DNA type 6/11, 16, and 18. And also immununohistochemical stain for panel of monoclonal cytokeratin (CK) antibodies were done. The result obtained is as fallows : 1. HPV DNA type 6/11 are detected in all dysplasias. HPV DNA type 16 and 18 are detected in squamous cell carcinomas. 2. Stratifed squamous epithelium of exocervix are positive staining for CK 1, CK 5/6, CK 8, CK 13, CK 14, and CK 19. HPV DNA type 6/11 infected cells are positive for CK 1, CK 13, CK 14, CK 17, CK 18, and CK 19. HPV DNA type 16/18 infected cells are positive for CK 1, CK 5/6, CK 10, CK 13, CK 14, CK 17, CK 18, and CK 19. In conclusion, HPV infection of uterine cervix is associated with change of normal cytokeratin expression pattern. Dysplastic cells are changed of CK 5/6, CK 8, CK 17 and CK18. Squamous cell carcinoma cells are changed of CK 8, CK 17, and CK 18.

8.3% Carbamide Peroxide 함유 펜 형 자가미백제인 BIancTis Forte의 색조개선 및 안전성에 관한 임상연구

이진경,민선홍,홍성태,오소람,정신혜,황영혜,유성엽,배광식,백승호,이우철,손원준,금기연 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

This clinical study evaluated the whitening effect and safety of polymer based-pen type BlancTis Forte(NIBEC) containing 8.3% carbamide peroxide. Twenty volunteers used the BlancTis Forte whitening agent for 2 hours twice a day for 4 weeks As a control. Whitening Effect Pen (LG) containing 3% hydrogen peroxide was used by 20 volunteers using the same protocol. The change in shade (ΔE^(*) color difference) was measured using Shadepilot™ (DeguDent) before, during and after bleaching (2 weeks, 4 weeks and postbleaching 4 weeks). A clinical examination for any side effects (tooth hypersensitivity or soft tissue complications) was also performed at each check-up. The following results were obtained. 1 Both the experimental and control groups displayed a noticeable change in shade (ΔE) of over 2 No significant differences were found between the two group (p>0.05) implying that the two agents have a similar whitening effect. 2 The whitening effect was mainly due to changes in a and b values rather than in L value (brightness). The experimental group showed a significantly higher change in b value thus yellow shade than the control(p < 0.05) 3 None of the participants complained of tooth hypersensitivity or soft tissue complications confirming the safety of both whitening agents 8.3%의 carbamide peroxide를 함유한 펜형 코팅용 미백제인 BlancTis Forte (NIBEC, Seosul & JinCheon)를 실험군으로, 3% hydrogen peroxide를 함유한글 Whitening Effect Pen (LG. Seoul) 제재를 대조군으로 각각 피험자 20명에게 2시간씩 1일 2회 제조사의 지시대로 치아표면에 4주간 적용하도록 지시하고 색조개선 효능과 안전성을 평가하였다. 미백 효과는 미백 전 및 미백 2주, 4주 및 미백 종료 4주 후에 Shadopilot™을 이용하여 색 변화를 측정하였고, 매 내원시기마다 모든 환자는 치수생활력 검사와 치주 및 치태 검사를 통해 부작용 여부 (치아과민증 및 구감 내 연조직의 부작용)를 기록한 후 다음과 같은 결과를 얻었다. 1.실험군 및 대조군의 색 변화량 (ΔE)은 2이상으로 인지할 수 있는 색 변화를 보였으며, 두 제품 간에는 통계적으로 유의한 차이를 보이지 않아 (p > 0.05) 유사한 미백효능을 나타냄을 알 수 있었다. 2,미백효과는 명도의 개선보다는 주로 a. b값의 변화에 의한 것으로 특히 실험군에서 b값의 변화, 즉 황색조의 개선효과가 대조군에 비해 유의성 있게 높은 것으로 나타났다 (p <0.05). 3.치아나 치은의 과민증이나 이상증상을 호소하는 피험자는 없어 두 제품 모두 안전성을 확인할 수 있었다.

Prospidin이 Mouse의 간장에 미치는 영향

이규식,정호삼,이민형,백두진,기춘석 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

Prospidin, a new alkylating agent, has antimitotic and anticancer effect. Incubation with prospidin increases the stability of DNA and suppresses the incorporation of uridine into RNA by 15-20 percent. Prospidin is cytostatic for suspension culture and cells are blocked in G₂ phase of the cell cycle. The authors have demonstrated the effect of prospidin on the liver of mice histologically and histochemically and observed the cellular changes, loss of pyroninophilic granules and accumulation of fat in the hepatic parenchymal cells. Sixty-five healthy mice of ICR strain, average weight being 20g, were divided into two groups; control and prospidin-treated groups. All experimental animals were administered foo and water ad libitum. Animals of the prospidin-treated groups were administered prospidine 500mg per kg of body weight intraperitoneally fter dilution in the water for injection. Animals of the control group were administered only water for injection. Prospidin treated animals were sacrificed at intervals of 6, 12, 24, 36 and 48 hours after administration of prospidin. Histological preparations were made by way of hematoxylin and eosin stain for the cellular changes, methyl green-pyronin stain for pyroninophilic granules and oil red O stain for fat accumulation in the mouse liver. The results obtained are as follows. 1. Cellular swelling associated with vesicular formation were observed in the hepatic parenchymal cells of entire hepatic lobule at 24 and 36 hours after administration of prospidin. 2. A marked reduction of the pyroninophilic granules were observed in the hepatocyte of he whole hepatic lobule at 24, 36 and 48 hours following drug administration. 3. Fat deposits in the hepatic lobule were seen at 36 hours after administration of prospidin. It is suggested that prospidin acts as a cytotoxic agent and causes cellular swelling with vesicular formation, loss of pyroninophilic granules, and fat accumulation in the parenchymal cells of the liver.

동물체모 및 장기 중 카드뮴의 방사화 분석

이기호,유용운 대한방사선 방어학회 1990 방사선방어학회지 Vol.15 No.2

백서의 장기 내 카드뮴의 축적분포를 중성자 조사에 의한 방사분석법으로 조사하였다. CdCl2를 1주일에 10mg /kg씩 급식되도록 매일 물병으로 투여하였으며 1주, 2주, 3주, 1개월, 2개월 및 3개월 기간동안 장기 복용케한 후 주요장기 및 체모를 시료로 하여 방사화 분석을 수행하여 아래와 같은 결론을 얻었다. 1) 115Cd을 방사추적자로 사용하여 24시간 경과 후 체내 카드뮴의 분포를 조사한 결과 혈액에 0.03%신장에 2.99% 간장에 3.50%를 나태내었다. 2) Cd은 혈액을 통해 신속히 순환되어 신장을 통하여 백설되나 간장과 체모에도 축적되는 결과를 나타냈다. 3) CdCl2를 급식케 한 실험군의 주요 장기별 Cd함량을 측정 비교한 결과를 이용하여 간장 내 Cd의 축적을 체모 내 Cd함량으로부터 진단 하는 지표로 사용이 가능케 되었다. Rats were ingested in drinking water 600mg/L of cadmium chloride solution during 3 months, then the distribution of Cd in major organs and hair were determined by neutron activation analysis. The results were as followings. 1. After administration for 24 hours'using 115cmCd as tracer, the distribution of blood was 0.03%, kidney 2.99% and liver 3.50% to determine with whole body counter. 2. Cd metal was rapidly excreted with kidney through blood and their accumulation appeared in liver and hair. 3. The comparative data to determine using neutron activation analysis, the content of cadmium of major organs in rats ingested of CdCl2 during 3 month were shown to increase significantly both hair and liver. Above facts, hair samples were able to use as the diagnostic index to evaluate the accumulation of cadmium in liver.

퍼지집합 理論을 利用한 構造物의 最適設計에 관한 硏究

이종호,홍영기,우호길 충남대학교 공업교육연구소 1999 論文集 Vol.22 No.1

There exists a vast amount of fuzzy information in both objective and constraint functions of the optimization problem. Also most engineering design problems require the consideration of multiple and conflicting objectives. To handle these problems, the approach based on both fuzzy set theory and Dempster-Shafer theory is presented in this paper. In other words, original partly fuzzy multi-objective optimization problem is defuzzified into a crisp generalized multi-objective optimization problem using fuzzy set theory. The crisp generalized multi-objective problem is then transformed into an equivalent single objective optimization problem using a Dempster-shafer theory. The resulting problem can be solved to seek the best compromise solution by a common optimization technique.