Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

濟州道 住民 蟯蟲感染의 疫學的 硏究

李根泰,李範珪,盧英俊,任世旭,李在興 醫藥係社 1955 綜合 醫學 Vol.12 No.12

복합적인 레지스트 패턴 붕괴현장의 역학적 해석

이형주,박준택,안일신,김옥근,오혜근 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

패턴의 선폭이 100nm이하로 작아짐에 따라 패턴이 부러지고, 휘고, 떨어져 나가는 현상이 발생하는데 이러한 현상을 패턴 붕괴 현상이라고 하며, 반도체 생산과 수율에 많은 영향을 미친다. 패턴 붕괴 현상은 리소그래피 과정 중 현상 후 세척액을 건조하는 과정에서 주로 발생하며 세척액의 표면장력에 의한 모세관 현상이 주된 원인으로 알려져 있다. 패턴 붕괴 현상을 시뮬레이션하기 위하여 패턴이 세척액에 의해서 균일한 압력을 받을 때와 집중 압력을 받을 때의 역학적 모델을 세우기 각각의 모델을 통하여 선폭과 패턴간의 거리에 따른 임계 높이/선폭비 (aspect ratio) 를 계산하여 실제 실험 결과와 일치하는 모델을 선정하였다. 또한 패턴 내부에 생기는 stress 에 의한 부러짐 현상과 패턴 과 레지스트 패턴 하부 층 사이의 접착력 부족으로 발생하는 떨어짐 현상을 해석하였다. High aspect ratio resist patterns with dimensions below 100 nm often bend, break or tear. These phenomena are generically called "resist pattern collapse". Pattern collapse is a very serious problem in fine patterning of less than 100 nm critical dimension (CD), so that it decreases the yield. In order to mechanically analyze this phenomenon and create its simulator, two models have been made and compared. In this paper, various approaches with various analyses are made to understand pattern collapse. Also, the critical aspect ratio for 100 nm node, that determines whether pattern collapse happens or not, can be calculated with these approaches. Finally, tear type caused by insufficiency of adhesion strength between the substrate and the resist is analyzed with a point of view of the surface free energy.

縱斷的 資料에서 본 다운症兒와 精神遲滯兒의 身體發育樣相에 관한 硏究

李相于,朴俊東,辛尙根,安起英,姜信範 東亞大學校 附設 스포츠科學硏究所 1994 스포츠科學硏究論文集 Vol.12 No.-

The purpose of this study was to clarify physical growth pattern from longitudinal data of the down's syndrome and the mentally retarded children, through comparing our results with those of the normal children. Measurements of body height, body weight, chest girth and sitting height on 16 down's syndrome boys(16girls), 21 mentally retarded boys(14girls) and 90 normal boys(97girls) from 1982 to 1992 were taken every year during 11 years. The results were as follows : 1. In body height growth pattern for down's syndrome and mentally retarded children, down's syndrome and mentally retarded children showed lower then normal children, and then, group difference was significantly started 12 and 13-years-old of age over(P<0.05∼P<0.01) 2. Down's syndrome boys were significantly higher then normal boys except 17 years-old of age in body weight(P<0.01). 3. Down's syndrome and mentally retarded girls were significantly higher then normal girls except 13~15 years-old of age in body weight(P<0.05∼P<0.01) 4. Down's syndrome boys were significantly higher then normal boys between all age intervals in chest girth (P<0.01) 5. Down's syndrome and mentally retarded girls were significantly higher then normal girls in chest girth(P<0.01) 6. Down's syndrome and mentally retarded children were significantly lower then normal children except 7 through 11 years-old of age in sitting height(P<0.05∼P<0.01)

e-Learning을 위한 SCORM 기반 동적 시퀀싱

이종근,김형일,김준태 東國大學敎 産業技術硏究院 2005 산업기술논문집 Vol.15 No.1

e-learning에서 실행 환경에 종속적인 학습 컨텐츠는 저작 비용을 증가시키는 문제를 발생시킨다. 이와 같은 문제점을 해결하기 위해 SCORM에서는 학습 컨텐츠의 공유와 재사용에 관한 표준안을 제시한다. e-learning에서 또 다른 문제점은 학습자의 학업 성취도 향상에 있다. 현재 e-learning에서는 정형화된 교육 절차에 따라 학습을 수행하고 종료하는 방식을 취함으로써 학습자의 학업 성취도를 향상시키지 못한다. 이와 같은 문제점을 해결하기 위해 SCORM에서는 학습에 대한 절차를 규정하는 시퀀싱을 활용하여 학습자의 학업 성취도를 향상시킨다. 일반적으로 시퀀싱 설계는 교수자나 학습 저작자가 담당하여 학습 프로그램을 규칙화한다. 정형화된 시퀀싱으로는 학습 집단이나 학습자의 특성을 분석하기 어려우며, 잘못된 시퀀싱이 설계되었을 경우엔 학습자들의 학습에 악영향을 미친다. 본 논문에서는 이와 같은 문제점을 해결하기 위해 동적 시퀀싱을 적용한 학습 시스템을 제안한다. 동적 시퀀싱에서는 학습자들의 평가점수가 시퀀싱에서 활용되는 기준점수에 반영되어 기준점수를 동적으로 변화시킨다. 기준점수를 동적으로 변화시킴으로 시퀀싱은 학습 집단이나 학습자들의 학습 능력에 맞게 동적으로 변화되어 학습자의 학습 성취도를 향상시킨다. The problem occurred with the growth of e-learning lies in the different implementation environment and the learning contents which are not shared and reused. To solve these problems, SCORM presents a standard specification for the sharing and reusing of the learning contents. Another problem with the e-learning comes from the degree of improvement on learning accomplishment of learners. The present e-learning approach cannot improve the degree of learning accomplishment of learners because the learning is carried out and finished by formulated education procedures. To solve this problem, SCORM improved the degree of learning accomplishment of learners by utilizing the sequencing which regulates the procedures for learning. The formulated sequencing is difficult to analyze the characteristics of the learning group, and if a wrong sequencing is designed, it is ineffective on the learning of learners. In this paper, we proposed a learning system which adopted a dynamic sequencing so as to solve this problem.

운동 중 지방 대사에 대한 고찰

이근일,백병주,김의영,김도준,조용철,정훈,조재혁 龍仁大學校 體育科學硏究所 2002 體育科學硏究論叢 Vol.12 No.1

Glucose-fatty acid cycle is very important because it explains that fatty acid influences glucose level. Glucose-fatty acid cycle is the key factor to understand how endurance training which aids fat oxidation and carbohydrate stored in skeletal muscle influence mitochondria level. However, it is not certain whether glucose-fatty acid cycle influences exercising skeletal muscle of human being. As an energy resource, training increases use of fatty acid, endurance ability, and oxidation of triglyceride. During high level training, catecholamine secretions and restraint of fatty acid use caused by insulin increases dramatically, this results in reduction of free fat acids and reunification of free fat acids into triglyceride. Training restrains insulin activity and reduces plasma acid density, however, elite athletes have high triglyceride-fatty acid cycle. Although enzyme's activities and mitochondira's density increase after training, it's not certain that enzyme's activities is major factor in restraint of fatty acid oxidation in exercising muscle cells. Stores of fat leads to increases of fat oxidation, performance and reduces use of carbohydrate, however, there is no absolute evidence to support these results.

알코올 사용과 스트레스와의 관련성

전진용,오동열,구민성,이준석,천근아,박웅섭,김상아 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.4

Objectives : This study was designed to assess the relationship between alcohol use and stress. Methods : The study subjects were 1,261 people who drink among the 2,565 people who lived in Gangneung. We investigated the sociodemographic characteristics, Short Form-12 (SF-12), Global Assessment of Recent Stress (GARS) and Alcohol Use Disorder Identification Test (AUDIT). Results : In Problem drinking group the GARS was significantly positively associated with the AUDIT (p=0.02). In Social drinking group sex (p<0.001) and age (p=0.02) is significantly associated with the AUDIT. Conclusion : This study showed the significantly positively association between stress and alcohol use. We think that the intervention and active management of the stress can prevent the alcohol problem.

인슐린 비의존성 당뇨병에 동반된 심상성 천포창 1 례

이상준,박진용,임태형,윤영호,권순대,남창욱,허규찬,박근용,구본식,김상표,장종억 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

저자들은 인슐린 비의존형 당뇨병 환자에서 통증성 구강내 미란 및 전신성 수포성 미란을 동반한 심상성 천포창 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Various skin conditions such as diabetic dermopathy, necrobiosis lipoidica diabeticorum, bullosis diabeticorum, granuloma annulare occur frequently in diabetes, although common lesions may be associated by chance. Pemphigus vulgaris(PV) is a blistering skin disease seen predominantly in elderly patients. This disorder is characterized by the loss of cohesion between epidermal cells with the resultant formation of intraepidermal blisters. These blisters rupture easily, leaving denuded areases that may crust and enlarge peripherally. In half or more of patients, lesions begin in the mouth: approximately 90% of patients have oromucosal involvement at some time during the course of their disease. But the relationship of this disorder with diabetes is not clear. We experienced a case of pemphigus vulgaris associated with NIDDM in 67 years old woman who was admitted due to painful oral ulcer and control of blood glucose, so we present this case with a review of literatures.