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José A.A. Santos,Antonio Augusto Fidalgo-Neto,Robson X. Faria,Ana Simo˜es,Andrea S. Calheiros,Ana Luiza Be´renger,Hugo C.C. Faria-Neto,Maria Raquel Figueiredo,Valber S. Luiz Frutuoso,Luiz Anastacio Al 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9
Recently, the P2X_7 receptor has been reported to be associated with chronic inflammatory and neuropathic pain. Because Rheedia longifolia extract has analgesic and anti-inflammatory activity, we evaluated the in vitro inhibitory potential of methanol extract and fractions from its leaves on the P2X_7 purinergic receptor. The activity of P2X_7 was studied with a dye uptake assay and with the whole-cell patch clamp technique in mouse peritoneal macrophages treated with methanol extract of R. longifolia leaves and fractions. The dye uptake was evaluated by flow cytometry and fluorescence microscopy. The R. longifolia extract and some fractions showed an inhibitory effect on the P2X_7 purinergic receptor in a dose-dependent manner. The ethyl acetate fraction exhibited the most potent inhibitory effects. The methanol extract and the butanol fraction showed the same inhibitory effects, despite their lower potency compared with the other fractions. The R. longifolia extract and some of its fractions may be anti-inflammatory because of their inhibitory effect on the P2X_7 receptor. Further investigation is needed to determine the pattern of inhibition and selectivity. Chromatographic analysis indicated the presence of bisflavonoids in the methanol extract fractions. A member of this chemical family is the most probable active compound responsible for the P2X_7 inhibitory effects present in the R. Longifolia extract and fractions.
Chemical Profile and Antinociceptive Efficacy of Rheedia longifolia Leaf Extract
José A.A. Santos,Andrea Calheiros,Diogo D. Nascimento,Ana Luiza R. Bérenger,Fábio C. Amendoeira,Hugo C. Castro-Faria-Neto,Maria Raquel Figueiredo,Luiz A. Alves,Valber da Silva Frutuoso 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9
Different species of the family Clusiaceae, including Rheedia longifolia, are used in folk medicine to treat inflammatory diseases. This family is largely distributed in tropical and subtropical areas of Brazil, but their chemical and pharmacological properties have been the subject of a few studies. In previous studies, we found that the aqueous extract from R. longifolia leaves presented important anti-inflammatory and analgesic activity. We investigated the chemical profile of R. longifolia and characterized the pharmacological effect of different chemically identified fractions in pharmacological models of neurogenic and inflammatory nociception. The pharmacological tests showed that oral treatment with aqueous crude extract and fractions of methanol extract of R. longifolia leaf induced a significant antinociceptive effect using von Frey filaments. In addition, the most polar fractions presented antinociceptive activity in a neurogenic model of nociception (capsaicin model). The chromatographic analysis indicated the presence of bisflavonoids in the fractions obtained from the methanol extract. These results suggest that bisflavonoids found in methanol-extracted fractions are involved in the inhibition of inflammatory and neurogenic nociception. It is important that the R. longifolia aqueous extract treatment inhibited ulcer formation induced by indomethacin, suggesting an anti-ulcerogenic activity closely associated with its analgesic effect.
José Miguel Campos,Ana Catarina Sousa,Pedro Olivério Pinto,Jorge Ribeiro,Miguel Lacueva França,Ana Rita Caseiro,Mariana Vieira Branquinho,Sílvia Santos Pedrosa,Carla Mendonça,Ana Brandão,José Domingos 한국생체재료학회 2019 생체재료학회지 Vol.23 No.1
Autologous bone remains the gold standard grafting substrate for bone fusions used for small gaps and critical defects. However, significant morbidity is associated with the harvesting of autologous bone grafts and, for that reason, alternative bone graft substitutes have been developed. In the present case series, a glass-reinforced hydroxyapatite synthetic bone substitute, with osteoinductive and osteoconductive proprieties, was applied. This synthetic bone substitute comprises the incorporation of P2O5-CaO glass-based system within a hydroxyapatite matrix, moulded into spherical pellets with 250-500 μm of diameter. A total of 14 veterinary clinical cases of appendicular bone defects and maxillary / mandibular bone defects are described. In all clinical cases, the synthetic bone substitute was used to fill bone defects, enhancing bone regeneration and complementing the recommended surgical techniques. Results demonstrated that it is an appropriate synthetic bone graft available to be used in veterinary patients. It functioned as a space filler in association with standard orthopaedic and odontological procedures of stabilization, promoting a faster bone fusion without any local or systemic adverse reactions. This procedure improves the animals’ quality of life, decreasing pain and post-operative recovery period, as well as increasing bone stability improving positive clinical outcomes.
José M. Marques,P.M. Carreira,J.E. Marques,H.I. Chaminé,P.E. Fonseca,F.A. Monteiro Santos,H.G.M. Eggenkamp,J. Teixeira 한국지질과학협의회 2010 Geosciences Journal Vol.14 No.4
The aim of this paper is to review the results of the assessment of low-temperature geothermal resources (issue temperatures between 41 and 77℃) that occur in the Portuguese mainland. For this purpose, a multidisciplinary approach, including geologic, tectonic, geochemical, geophysical and isotopic (2H, 18O, 13C, 3H and 14C) techniques, was applied in order to update local and/or regional conceptual circulation models. Three case studies of N-Portugal are presented and discussed. This paper describes different low-temperature geothermal waters presenting similar hydrogeological conceptual models but rather different geochemical signatures (e.g., HCO3–Na with pH 8, HCO3/Na/CO2-rich with pH 7 and HCO3–Na with pH 9, type waters). In fact, in the studied low-temperature geothermal systems, local/regional high altitude sites associated with highly fractured rocks play an important role in conducting the infiltrated meteoric waters towards the discharge zones near the Spas. The discharge zones are mainly related to the intersection of major regional fault lineaments (and conjugate structures), responsible for creating the mineral waters ascent. In some cases, geochemical and isotopic data point out to the existence of anthropogenic contamination of some geothermal spring waters related to the intense use of fertilizers in areas of widespread agricultural practices
Luis A. Trujillo-Cayado,M. Carmen Alfaro,Jenifer Santos,Nuria Calero,José Muñoz 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.66 No.-
This contribution deals with the development of emulsions formulated using thyme essential oil and a new biomass-derived surfactant. In addition, this work extends our knowledge concerning the factors that can influence stability and droplet size distributions of microfluidized emulsions, such as the geometry of the rotor–stator used and the homogenization rate in the primary homogenization. Stable thyme oil-in-water emulsions (30 wt%) containing submicron droplets were formed. Interestingly, laser diffraction results reveal that mean droplet sizes are mainly controlled by homogenization rates and polydispersity by the rotor–stator geometry used in the first step of homogenization. In addition, higher droplet sizes for pre-emulsions seem to be a key factor in order to reduce both the degree of recoalescence and the size of the droplets in the second homogenization step. Furthermore, higher droplet sizes in the pre-emulsion favour higher physical stability of the final emulsions. Finally, this research highlights the importance of controlling primary homogenization conditions for the physical stability of microfluidized emulsions that contain natural ingredients.
Bursectomy, Curettage, and Chemotherapy in Tuberculous Trochanteric Bursitis
Luis R. Ramos-Pascua,José A. Carro-Fernández,José A. Santos-Sánchez,Paula Casas Ramos,Luis J. Díez-Romero,Francisco M. Izquierdo-García 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.1
We presented three patients with trochanteric tuberculosis and described the clinical and imaging findings of the infection. Histology revealed a necrotizing granulomatous bursitis and microbiology confirmed tuberculosis. All cases were successfully treated with bursectomy and curettage of the trochanteric lesion and antituberculous chemotherapy including isoniazid, pyrazinamide, rifampicin, and ethambutol.
Luciana S. Aragão-França,Viviane C. J. Rocha,Andre Cronemberger-Andrade,F. H. B. Costa,José Fernandes Vasconcelos,Daniel Abensur Athanazio,Daniela Nascimento Silva,E. S. Santos,Cássio Santana Meira,C. 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4
Purpose: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.
Letícia Z. Oliveira,Vera F. M. Hossepian de Lima,Marcelo A. Levenhagen,Ricarda M. dos Santos,Terezinha I. Assumpção,José O. Jacomini,André F. C. de Andrade,Rubens P. de Arruda,Marcelo E. Beletti 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.3
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the 36 samples analyzed ensured that a large number of cells were investigated. The data were subjected to analysis of variance at a level of significance of 5%. Percoll centrifugation reduced the percentage of sperm exhibiting normal acrosomes (from 61.77 to 30.24%), reduced the percentage of sperm presenting atypical acrosome reactions (from 28.38 to 4.84%) and increased the percentage of sperm exhibiting damage in the acrosome (from 6.14 to 64.26%). The percentage of sperm with typical acrosome reactions was not significantly different before (3.70%) and after (0.67%) centrifugation. TEM distinguished four different types of acrosomal status and enabled ultrastructural characterization of acrosomal injuries. The percentage of sperm exhibiting normal acrosomes decreased and damage in the acrosome was the most frequent acrosomal injury with the Percoll gradient centrifugation protocol utilized.