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Alexandro Cézar Faleiro,Pedro Alexandre Valentim Neto,Thaysi Ventura de Souza,Marisa Santos,Ana Carolina Maisonnave Arisi 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.2
Plant growth-promoting rhizobacteria (PGPR) are considered to have a beneficial effect on host plants. Azospirillum brasilense is a diazotroph associated with important agricultural crops such as maize and wheat, and has been used as model organism to investigate associative plant growth promotion. In this study, we have employed scanning electronic microscopic to observe the presence of bacterium on the root surface. Two-dimensional gel electrophoresis and mass spectrometry were used to analyze the changes in protein profile of maize roots in response to A. brasilense strain FP2 7 days after inoculation and we observed 46 differently expressed protein spots. We report the identification of three of these proteins.
Fla´via V. Santa-Cecı´lia,Ge´rsika B. Santos,Carolina N. Fuzissaki,Priscilla B.M.C. Derogis,Lissara A.S. Freitas,Vanessa S. Gontijo,Paulo C. Stringheta,Tanus J. Nagem,Maı´sa R.P.L. Brigaga˜o,Marcelo H 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.2
Despite defenses by polymorphonuclear neutrophils in the host against invading agents, overproduction of oxidant species by phagocytes can lead to damage in the surrounding tissues. Several benzophenones have been shown to possess anti-inflammatory properties. The effect of the natural benzophenone 7-epiclusianone isolated from leaves of Garcinia brasiliensis was investigated by using in vitro antioxidant and ex vivo anti-inflammatory assays, focusing on the neutrophil respiratory burst and on the biochemical pathways involved. The bioactive extract, 7-epiclusianone, showed low in vitro antioxidant activity as evaluated by the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, the reducing power test,or the chelating power assay. However, the benzophenone displayed potent activity in the ex vivo model of the neutrophil respiratory burst, inhibiting the generation of superoxide anions in a dose-dependent manner. When the respiratory burst was triggered by N-formyl-methionyl-leucyl-phenylalanine, a chemotactic peptide, the 50% effective concentration (EC50) was 41.18 lg/107 cells. When phagocytes were stimulated directly through protein kinase C via phorbol, the EC50 was 34.3 lg/106cells. The results indicated that 7-epiclusianone was able to down-regulate inflammatory phagocyte superoxide anion release through a mechanism controlled by tyrosine protein phosphorylation and by a direct stimulation of protein kinase C. These findings could lead to new therapeutic approaches for inflammation management and the development of new drugs.