Kim's Femoral Stem의 임상적 고찰

김영민(Young Min Kim),김희중(Hee Joong Kim),진종수(Jong Soo Jin),문영완(Young Wan Moon) 대한정형외과학회 1991 대한정형외과학회지 Vol.26 No.5

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

過剩要素를 갖고 있는 RLC回路網에 對한 狀態方程式의 表現方法

金秀重 慶北大學校 文理科大學 1973 工大硏究誌 Vol.2 No.-

A method for writing state equations for the RLC network with excess elements is presented. When an RLC network has excess elements, state epuations in general form is obtained, if the each excess capacitor C can be replaced by an element with voltage e and carrent Ce˙, and also the each excess inductor L with cerrent i and voltage Li. In this case the voltage e is found from the capacitor loop, and the current i from the inductor cutset.

난소암조직 Acid Deoxyribonuclease의 분리와 성상에 관한 연구

김두상,김영신,고재경,한중수 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

In order to find out a possible role of acid deoxyribonuclease (DNase) in carcinogenesis of the ovary, activity of the enzyme was measured and the nature of the enzyme was studied in serous cystadenocarcinoma and endodermal sinus tumor of the ovary following the purification of the acid DNase in the tumor tissue of the ovary. (1)The acid DNase activity was greatly increased in the tumor tissues of the ovary, serous cystadenocarcinoma and endodermal sinus tumor tissues, while neutral and alkaline DNase activities were unchanged in the tumor tissues. This may indicate that the acid DNase can be used as a biochemical marker for the ovary tumors. (2)Proteins in the tumor tissues of the ovary were separated by a DEAE-cellulose column chromatography into 7 peak, respectively, of which one peak protein each appeared to be specific for serous cystadenocarcinoma and endodermal sinus tumor. (3)Acid DNases in the tumor tissues of the ovary were isolated into a single peak, respectively. The size of peak in the tumor tissues was greater than that in the control tissue of the ovary, indicating that the acid DNases in the tumor tissues were activated, but not specific for the tumors. (4)Acid DNases in the tumor tissues of the ovary were partially purified by centrifugation and a DEAE-cellulose column chromatography. The purified enzyme was highly active against double stranded DNA (ds-DNA), even though some activity was found against single stranded DNA (ss DNA). Observations that acid DNase from serous cystadenocarcinoma tissue was highly active and specific to ds DNA compared with ss DNA suggested that the enzyme might play a role in transforming normal ovarian cells into cancer cells.

속리산국립공원의 이용활성화 방안에 관한 연구

김시중,김수경 우송대학교 1998 우송대학교 논문집 Vol.3 No.-

Currently, the number of visitors of Sokri-Mountain National Park is dramatically decreasing compared to that of last year. The purpose of this study is to bring up problems of Sokri-Mountain National Park and to find out the solution on increasing the number of visitors. According to this study, the most urgent countermeasure is to relax restrictions for the development on Boupju Tample area.

경희대학교 수원 캠퍼스의 교문디자인 연구

김동찬,이윤수,박중구,황세현,김명기 경희대학교 디자인연구원 2000 예술· 디자인학연구 Vol.3 No.1

The Suwon Campus of Kyunghee University was decided to rebuild a university gate to make new image of the university for a new millenium. The purpose of this study is to design a university gate not only for a gate but also for a commemorative environmental model which presents a spirit and idea of the Kyunghee University. The entire design of the gate has a renaissance style, the design of it's center has a triumphal arch form which cars can go through, and the design of it's side has a corridor form which pedestrians can walk. The gate is designed to present the spirit for human and world peace which Kyunghee University intends.

Berkeley Physics Lab. Part C 실험의 특징과 문제점

김기완,김수중 경북대학교 공과대학 1973 工大硏究誌 Vol.2 No.-

Following the development of the new P.S.S.C. physics course for high school and college, several new physics course for undergraduates were developed as follows: Berkeley Physics Course, MIT course, Cal. Tech. Introductory Physics Course, etc. Along with these new courses new physics laboratories have evolved. As a subsequent paper, therefore, we intend to make a brief review of general characteristics of the Berkeley Physics Laboratory Course developed by A.M. Portis in 1962, and discuss about several problems encountered in experiments contained in Part C of the course.

骨盤腔內 巨大 橫紋筋肉腫 1 例

金瑩洙,河泰原,朴滿洙,姜瞬求,李重禾,尹栗老,薛鍾求 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

Rhabdomyosarcoma, which is a very rare and highly malignant disease in urologic field, has three categories-pleomorphic, alveolar, embryonal, and may arise from any part of the body that contains embryonal mesenchyme, whether striated muscle tissue is present or not. The common site in the genitourinary tract are prostate, bladder, and vagina. Herein we experienced a rhabdomyosarcoma of the pelvic cavity and reviewed literature briefly.

GH_3 세포(rat somatomammotropic tumor cell)에서 TRH가 Phospholipase D 활성에 미치는 효과

김동선,김태화,이창범,안유헌,윤미섭,한중수 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: GH_3 세포는 TRH에 반응하여 세포막의 수용체와 G 단백, PI-PLC, PKC를 활성시켜 성장호르몬 및 프로락틴을 분비한다고 알려져 있다. PLD는 phosphatidylcholine을 phosphatidic acid(PA)와 choline으로 가수분해하는 효소로서 세포의 증식과 호르몬 분비에 관여한다. 본 연구는 GH_3 세포에서 아직 알려지지 않은 TRH의 PLD 활성에 대한 영향을 규명하고자 하였다. 방법: GH_3 세포를 1.5×10^6씩 분주하고 [^3H] myristate로 표지한 다음에 0.3% 알코올로 전 처치하였다. TRH 등의 시험제를 처치한 후에 메탄올로 반응을 종결하고 세포에서 총 지질을 추출하였다. PLD 활성은 박층크로마토그래피를 이용하여 총 [^3H] phospholipid에서 [^3H] phosphatidylethanol의 비율로 구하였다. 결과: TRH (1μM)의 처치 시에 PLC 활성은 44배 증가하였다. PLD 활성은 TRH (1μM), mastoparan (5μM), PMA(500nM)를 30분간 처치 시에 각각 1.9, 1.5, 2.2배 증가하였다. TRH(1μM)의 시간에 따른 PLD 활성 변동은 15, 30, 60, 120, 240분에 각각 142%, 170%, 172%, 160%, 115%의 증가를 보였다. 결론: GH_3 세포에서 TRH의 호르몬 분비와 세포증식의 신호전달 기전으로서는 PLC 활성뿐 아니라 PLD의 활성도 관여함을 시사한다. Backgroud: GH_3 cells are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone (TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phospholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in cellular proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in GH_3 cells, we investigated the activities of PLC and PLD in GH_3 cells treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methos : GH_3 cells were labeled with [^3H] myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by measuring [^3H] phosphatidylethanol from the [^3H] phospholipid using thin layer chromatography. Results : TRH (1μM) stimulated the PLD activity by 44-fold over that of the control values. TRH (1μM), mastoparan (5μM), and PMA (500μM) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls, The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1μM) were 142%, 170%, 172%, 160% and 115%, respectively. Conclusion : These results suggest that TRH stimulates not only PLC activity, but also the PLD activity in GH_3 cells (J Kor Soc Endocrinol 17:465∼472, 2002).

수리·통계모형에 의한 설계 갈수량 추정법 개발

김운중,김영균,문수회 朝鮮大學校 建設技術硏究所 2005 建設技術硏究 Vol.25 No.1

The method of a frequency analysis which takes advantage of annual low flow data is extensively used in our country when the design low flow is estimated. It is necessary to secure the measurement record of a long-term in this method and therefore it is not proper to apply this method for cases of measurement record of a short-term or an ungaged outlet. In work-site, generally, the design low flow of unmeasured station is determined by the secondhand method which utilizes the area ratio between the gaging station and the unmeasured station. However, this method using the area ratio is unreal and unreliable because the low flow that is different from the flood flow is affected by the topographical, the geological and the basin characteristics. A new proposal that estimates the design low flow by using the hydraulic-statistical model is made in this paper. A method the return period can be converted into the recession time is derived under an assumption that the dry spell is similar to the low-flow runoffs event. To induce the method(recession time model), two kinds of models are employed; One is the type I extremal value distribution as the dry spell model, and the other is the exponential linear recession equation as the low-flow runoffs model. The climate conditions, the watershed and the runoff characteristics are adopted as the parameters of the recession time model. Meanwhile, a recession model that can estimate the design low flow in the gaging station or the unmeasured station is made by using the results of the recession time model. The initial discharge and the recession constant are used as the parameter of the recession model. The present model derived from this study is applied into the outlet basin of Yongdam gaging station, and the estimated design low flow for the return period of 10 year is obtained as 2.14m3/sec which corresponds to 97% of standard low flow (2.22m3/sec). Resultantlv, it is concluded that the present model proposed in this paper can be effectively used as the alternative for estimation of the design low flow.