The role of amino acid-induced mammalian target of rapamycin complex 1(mTORC1) signaling in insulin resistance

윤미섭,최철수 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

Mammalian target of rapamycin (mTOR) controls cell growth and metabolism in response to nutrients, energy, and growth factors. Recent findings have placed the lysosome at the core of mTOR complex 1 (mTORC1) regulation by amino acids. Two parallel pathways, Rag GTPase-Ragulator and Vps34-phospholipase D1 (PLD1), regulate mTOR activation on the lysosome. This review describes the recent advances in understanding amino acid-induced mTOR signaling with a particular focus on the role of mTOR in insulin resistance.

[2014년 제56회 사법시험 합격] “성실하게 생활하셨다면 합격하실 겁니다.”

윤미섭 고시계사 2015 考試界 Vol.60 No.2

사적 경계 영역에서 나타나는 공공성에 관한 연구

윤미섭,이영수 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

The purpose of this study was to redefine the publicity and analysis how to operate in contemporary society. Because of relation inside and outside, privacy and publicity, interface is important in city landscape. In this buffer zone it become limitless as liminal space. We can percept another person so though this feel the publicity in this space. It indicates that the center of awareness shift from body to consciousness and bridge the grab between exterior and interior not though materials or design on special devices but though the plan and though flowing nature. So it's so simple and pure. After all, consciousness of the stranger extend to percept self-consciousness in soft boundary.

내열성 제한효소인 Svi Ⅰ을 생성하는 Streptomyces 분리균주의 수리 동정

배무,윤미섭,김형태,이계준,Bae, Moo,Yun, Mi-Sub,Kim, Hyoung-Tae,Lee, Kye-Joon 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

새로운 제한효소인 Svi I을 생성하는 Streptomyces속 한 균주(D2-5)를 토양으로부터 분리하여 형태적 관찰 및 수리동정을 실시하였다. 50개의 분류 단위 형질을 분석하였고 이 실험결과를 TAXON program에 적용하여 종의 수리 동정을 실시하였다. 그 결과 분리균 D2-5는 Streptomyces의 제 18 주군집에 속하며 이 군집에 속하는 S. violochromogenes와 유사성이 가장 높은 것으로 나타났다. 따라서 분리주는 Streptomyces violochromogenes의 한 균주로 동정하였다. Numerical identification was carried out for an isolate of Streptomyces D2-5 producing a new restriction endonuclease Svi I. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. the isolate was best matched to Streptomyces violochromogenes in the major cluster 18 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces violochromogenes.

내열성 제한효소인 Svi I을 생성하는 Streptomyces 분리균주의 수리 동정

배무,윤미섭,김형태,이계준 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.4

새로운 제한효소인 Svi I을 생성하는 Streptomyces속 한 균주(D2-5)를 토양으로부터 분리하여 형태적 관찰 및 수리동정을 실시하였다. 50개의 분류 단위 형질을 분석하였고 이 실험결과를 TAXON program에 적용하여 종의 수리 동정을 실시하였다. 그 결과 분리균 D2-5는 Streptomyces의 제 18 주군집에 속하며 이 군집에 속하는 S. violochromogenes와 유사성이 가장 높은 것으로 나타났다. 따라서 분리주는 Streptomyces violochromogenes의 한 균주로 동정하였다. Numerical identification was carried out for an isolate of Streptomyces D2-5 producing a new restriction endonuclease Svi I. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces violochromogenes in the major cluster 18 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces violochromogenes.

PLGA Nanoparticle의 제조시 유기용매 종류의 영향

손지은,이동일,윤미섭,송기창,안양규,최은정 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.1

Rapid Analysis of Proteomic Biomarkers Expressed in Human Endometrial Stromal Cells during Decidualization

오경진,최혜진,윤미섭,황정혜,장성렬,김용석,한중수 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10

Decidualization of human endometrial stromal (ES) cells plays a critical role in successful uterine implantation. Therefore, monitoring of the behavior of human ES cells may provide the clue for early detection of a uterine abnormality such as sterility and abortion. Monitoring of decidualization in vitro cell culture system fundamentally depends on expression of the definite biomarkers. In this study, we tried to uncover novel marker proteins of 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP)-induced decidualization in human ES cells using the surface enhanced laser desorption/ionization timeof-flight mass spectrometry (SELDI-TOF-MS). Samples were divided into three groups; control human ES cells (n=7), ES cells treated with 8-Br-cAMP (n=7 per each treatment, treated for 3, 6, 9, or 12 days), and cells from which 8-Br-cAMP was withdrawn for 3 days (n=7) or 6 days (n=7) after 8-Br-cAMP treatment for 6 days. Differential expressions between non-decidual control cells and 8-Br-cAMPinduced decidual cells were observed in the peaks of 9787.058 Da, 10115.45 Da, and 24031.25 Da, detected by H4 ProteinChip, and in the peaks of 10833.08 Da, 22440.88 Da, and 32777.38 Da, detected by CM10 ProteinChip. The expression patterns of these decidual markers are expected to provide invaluable information in monitoring cellular development, and further identification of these proteins may hopefully offer precious means for clinical research and therapeutic purposes.

PLGA Nanoparticle의 제조시 유기용매 종류의 영향

손지은,이동일,윤미섭,송기창,안양규,최은정 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2

PLGA[Poly(lactic-co-glycolic acid)]는 생분해성 고분자로써 인체 내의 무해성과 안전성을 장점으로 약물전달시스템(DDS)에 많이 사용된다. PLGA는 Poly lactic acid(PLA)와 Poly glycolic acid(PGA)의 성분 비율에 따라 인체 내에서 분해되는 속도가 달라지므로 약물체가 방출되는 속도를 조절할 수 있는 특징을 지니고 있다. 본 연구에서는 기존의 실험방법에서 나타나는 독성용매의 사용, 낮은 수율 등의 단점을 보완할 수 있는 Emulsification-Diffusion방법을 이용하여 PLGA를 유기용매에 녹여 O/W Emulsion을 형성한 후, 과량의 물을 더하여 100nm 이하의 nanoparticle을 얻었다. 이 과정 중 PLGA를 용해시키는 유기용매의 종류를 변화시켜 유기용매 종류 변화가 얻어진 nanoparticle의 크기 및 형상 등의 물성에 미치는 영향을 살펴보았다. 감사의 글: 본 연구는 한국과학재단 목적기초연구(R01-2003-000-1-720-0) 지원으로 수행 되었음.

Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization

백미옥,송해인,한중수,윤미섭 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Human endometrium decidualization, a differentiation process involving biochemical and morphological changes, is a prerequisite for embryo implantation and successful pregnancy. Here, we show that the mammalian target of rapamycin (mTOR) is a crucial regulator of 8-bromoadenosine 3’,5’-cyclic monophosphate (8-Br-cAMP)-induced decidualization in human endometrial stromal cells. The level of mSin1 in mTOR complex 2 (mTORC2) and DEPTOR in mTOR complex 1 (mTORC1) decreases during 8-Br-cAMP-induced decidualization, resulting in decreased mTORC2 activity and increased mTORC1 activity. Notably, DEPTOR displacement increases the association between raptor and insulin receptor substrate-1 (IRS-1), facilitating IRS-1 phosphorylation at serine 636/639. Finally, both S473 and T308 phosphorylation of Akt are reduced during decidualization, followed by a decrease in forkhead box O1 (FOXO1) phosphorylation and an increase in the mRNA levels of the decidualization markers prolactin (PRL) and insulin-like growth factor-binding protein-1 (IGFBP-1). Taken together, our findings reveal a critical role for mTOR in decidualization, involving the differential regulation of mTORC1 and mTORC2.

GH_3 세포(rat somatomammotropic tumor cell)에서 TRH가 Phospholipase D 활성에 미치는 효과

김동선,김태화,이창범,안유헌,윤미섭,한중수 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: GH_3 세포는 TRH에 반응하여 세포막의 수용체와 G 단백, PI-PLC, PKC를 활성시켜 성장호르몬 및 프로락틴을 분비한다고 알려져 있다. PLD는 phosphatidylcholine을 phosphatidic acid(PA)와 choline으로 가수분해하는 효소로서 세포의 증식과 호르몬 분비에 관여한다. 본 연구는 GH_3 세포에서 아직 알려지지 않은 TRH의 PLD 활성에 대한 영향을 규명하고자 하였다. 방법: GH_3 세포를 1.5×10^6씩 분주하고 [^3H] myristate로 표지한 다음에 0.3% 알코올로 전 처치하였다. TRH 등의 시험제를 처치한 후에 메탄올로 반응을 종결하고 세포에서 총 지질을 추출하였다. PLD 활성은 박층크로마토그래피를 이용하여 총 [^3H] phospholipid에서 [^3H] phosphatidylethanol의 비율로 구하였다. 결과: TRH (1μM)의 처치 시에 PLC 활성은 44배 증가하였다. PLD 활성은 TRH (1μM), mastoparan (5μM), PMA(500nM)를 30분간 처치 시에 각각 1.9, 1.5, 2.2배 증가하였다. TRH(1μM)의 시간에 따른 PLD 활성 변동은 15, 30, 60, 120, 240분에 각각 142%, 170%, 172%, 160%, 115%의 증가를 보였다. 결론: GH_3 세포에서 TRH의 호르몬 분비와 세포증식의 신호전달 기전으로서는 PLC 활성뿐 아니라 PLD의 활성도 관여함을 시사한다. Backgroud: GH_3 cells are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone (TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phospholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in cellular proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in GH_3 cells, we investigated the activities of PLC and PLD in GH_3 cells treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methos : GH_3 cells were labeled with [^3H] myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by measuring [^3H] phosphatidylethanol from the [^3H] phospholipid using thin layer chromatography. Results : TRH (1μM) stimulated the PLD activity by 44-fold over that of the control values. TRH (1μM), mastoparan (5μM), and PMA (500μM) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls, The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1μM) were 142%, 170%, 172%, 160% and 115%, respectively. Conclusion : These results suggest that TRH stimulates not only PLC activity, but also the PLD activity in GH_3 cells (J Kor Soc Endocrinol 17:465∼472, 2002).