Associations of serotonergic genes with poststroke emotional incontinence

Kim, Jae‐,Min,Stewart, Robert,Kang, Hee‐,Ju,Bae, Kyung‐,Yeol,Kim, Sung‐,Wan,Shin, Il‐,Seon,Kim, Joon‐,Tae,Park, Man‐,Seok,Cho, Ki‐,Hyun,Yoon, Jin‐ John Wiley Sons, Ltd 2012 INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY Vol.27 No.8

<P><B>Objectives</B></P><P>Poststroke emotional incontinence (PSEI) has been associated with serotonergic dysfunction. Polymorphisms of serotonin transporter (5‐HTT) and serotonin 2a receptor (5‐HTR2a) genes may regulate serotonergic signaling at brain synapses, and this study was to investigate associations with PSEI in an East Asian population.</P><P><B>Methods</B></P><P>In 276 stroke cases, PSEI was diagnosed by Kim's criteria. Covariates included age, gender, education, history of depression or stroke, current depression, and stroke severity and location. Genotypes were ascertained for 5‐HTT gene‐linked promoter region (5‐HTTLPR), serotonin transporter intron 2 variable number tandem repeat, 5‐HTR2a 1438A/G, and 5‐HTR2a 102 T/C. Associations with PSEI were estimated by using logistic regression models, and gene–gene interactions were investigated by using the generalized multifactor dimensionality reduction method.</P><P><B>Results</B></P><P>PSEI was present in 37 (13.4%) patients. The 5‐HTT gene‐linked promoter region <I>s</I>/<I>s</I> genotype was independently associated with PSEI. No associations with STin2 VNTR and 5‐HTR2a genes were found, and no significant gene–gene interactions were identified.</P><P><B>Conclusions</B></P><P>Stroke patients with 5‐HTTLPR <I>s</I> allele had higher susceptibility to PSEI, which underlines the potential role of serotonergic pathways in its etiology. Copyright © 2011 John Wiley & Sons, Ltd.</P>

Effect of Developmental Lead Exposure on the Expression of Hippocampal NMDA Receptor Subunit mRNA

Kim, Tae-Wan,Chung, In-Sung,Bae, Jae-Hoon,Shin, Dong-Hoon,Lee, Mi-Young,Kim, Joon-Sik 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

목적: in vivo 및 vitro에서 해마 신경세포의 발생단계별 NMDA 수용체 아단위 mRNA 발현에 대한 연 폭로 영향을 알아보고자 하였다. 방법: 흰쥐 해마 신경세포의 발생단계별 NMDA 수용체 NR2A, NR2B 아단위 mRNA 발현에 대한 연의 영향은 정상군과 연 폭로군의 출생 후 7일, 14일, 22일 흰쥐의 해마에서 in situ hybridization으로 mRNA 발현 정도를 densitometer로 측정하여 비교하였고, 연과 NMDA 의 세포독성은 해마 신경세포 일차배양 후 도립현미경을 이용한 형태학적인 관찰과 LDH 활성도를 이용하여 측정하였다. 결과: 연 과 NMDA 에 의한 세포독성에 대한 in vitro 실험에서 형태학적 소견과 LDH 활성도에서 해마 미성숙 신경세포와 성숙 신경세포사이의 차이가 있었으므로, 연과 NMDA 독성효과는 해마 신경세포의 발달 단계에 따라 차이가 있다. 정상군의 해마에서의 NR2A mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나, NR2B mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나 NR2B mRNA 발현은 연령의 증가에 따른 변화가 없었다. 연 폭로에 희한 NR2A mRNA 발현은 유의하게 감소하였으나(p<0.05), NR2B mRNA 발현은 변화가 나타나지 않았다. 만성적 연 폭로는 NR2A를 포함하는 NMDA 수용체를 감소시킬 수 있음을 알 수 있다. 결론: 연은 해마신경세포의 발생단계에서 NMDA 수용체 아단위 특히 NR2A mRNA 발현의 변화를 야기하여 시냅스 신호 전달에 영향을 나타냄을 알 수 있었다. Hippocampus

5세 남아의 Influenza 종격동기종 및 대엽성 폐렴 1례

오희성,한유진,연은경,선동신,송준환,박경배,박준수,김영통,남해선,최영진,김용배 순천향의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.14 No.3S

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G+C 함량이 높은 Primer를 사용하는 중합효소 연쇄반응에서 변성제가 미치는 영향

김영미,안준환,김종배,엄용빈 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

G+C 함량이 높은 primer를 이용한 중합효소 연쇄반응을 실시하는 경우 높은 annealing 온도로 인하여 특이 염기서열의 합성정도가 매우 미약하게 나타나는 경우가 많이 있다. 이와 같은 문제점을 보완하기 위하여 glycerol, formamide 및 dimethyl sulfoxide (DMSO) 등의 변성제를 반응용 완충액에 첨가하고 중합효소 연쇄반응을 실시하여 그 결과를 비교 검토하였다. G+C 함량이 낮은 Borrelia burgdorferi의 Ly1 유전자의 primer set인 Bb 679와 Bb 680를 이용한 중합효소 연쇄반응에서는 변성제 첨가에 따른 합성 DNA의 양의 변화가 뚜렷하지 않았다. 그러나 G+C 함량이 높은 primer set인 Mycobacterium paratuberculosis의 IS900 유전자의 IS900/150C와 IS900/921를 이용한 중합효소 연쇄반응을 유도한 경우에는 변성제를 첨가함에 따라 합성된 DNA의 양의 증가가 뚜렷하였으며, 2.5% glycerol과 1.25% formamide를 혼합 첨가한 경우와 또는 2.5% DMSO를 반응용 완충액에 첨가하였을 때 비특이적인 증폭비율이 낮아 특이 염기서열의 합성 결과가 양호한 것으로 나타났다. Poor yields of amplified DNAs could be resulted in polymerase chain reaction(PCR) processes with G+C-rich DNA primers because of their high Tm values. To maximize the yields of amplification in PCR processes with G+C-rich primers, we compared the yields of amplified DNA fragments according to the concentrations of specific denaturants added to the reaction mixture of PCR system. With addition of the mixture of 2.5% glycerol and 1.25% formamide, or 2.5% dimethyl sulfoxide to the reaction cocktail, respectively, remarkable increases in the yields of amplified DNA fragments were not observed in the PCR systems with G+C-low primers of Lyl chromosomal gene from Borrelia burgdorferi but observed in the PCR system with G+C­rich primers of IS900 gene from Mycobacterium paratuberculosis. Although we were not practically able to discriminate the yields of PCR DNAs according to the concentrations used in this study, addition of the mixture of 5% glycerol and 2.5% formamide, or 5% DMSO tended to increase the production of extra bands.

이하선에 발생한 지방종

김준배,장동수,배정수 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

This is a case report on the lipoma of the parotid gland. Lipomas are common soft tissue neoplasms found only rarely in the region of the parotid gland, and therefore often not considered in the initial differential diagnosis of a mass presenting in this region. Clinically they can be confused with other being lesions and there was no reliable methods to make a preoperative diagnosis of the lipoma until 1980's; however, CT allows a specific diagnosis to be made in virtually all case. We experienced that CT imaging permit the preoperative diagnosis of the lipoma, so could remove it preserving the parotid gland.

비신경교종 1례

김재욱,이배혁,오일준,전경명 釜算慶南耳鼻咽喉科學會 1993 임상이비인후과 Vol.4 No.2

PSPICE를 이용한 수정된 Chua회로의 카오스 통신 시뮬레이션

김성곤,배준호,호인석,최희주,변건식 東亞大學校 附設 情報通信硏究所 1999 情報通信硏究所論文誌 Vol.7 No.1

Recently, there has been much interest in utilizing chaotic circuits to implement a secure communication. Although Chua's circuit is the simplest chaotic circuit, it has complex chaotic dynamic porperties. The modified Chua's circuit has two additional components more than Chua's cirecuit and makes the circuit equations become fore-dimensional instead of three-dimensional, it makes Chua's circuit more flexible. In this paper, we use two modified Chua's oscillators to simulation on the chaos control and synchronizaion. The simulation results show that the modified Chua's oscillator has good chaotic control and sunchronization. And we have successfully carried out the basic esperiment on the chaotic secure communication.

소방호스 밀착강도시험방법에 관한 연구

김준배,백창선,김학진 한국화재소방학회 2001 한국화재소방학회논문지 Vol.15 No.1

소방호스의 밀착강도 시험방법을 개선하기 위하여 5개국(한국, 미국, 영국, 중국, 일본), 7개의 기술기준(KOFEIS, UL, NFPA, BS, 중국기술기준, JIS, 일본검정기술기준)을 비교 고찰하고, 국내에서 생산되는 소방호스를 무작위로 발췌하여 밀착강도시험을 실시하였다. 밀착강도시험 및 기술기준 비교 고찰을 통하여 밀착강도시험은 소방호스의 호칭별로 시험시효의 크기와 박리시험속도 및 판정기준을 세분화함으로서 소방호스의 밀착강도를 개선시킬 수 있다는 결론을 얻었다. In order to improve the adhesion test methods for fire hose, 7kinds of stands (KOFEIS, UL, FM, NFPA, BS, Chinese standard, JIS, JFEII standard) un 5 countries (Korea, USA, UL, China, Japan) were compared and adhesion tests were conducted by using the samples choosed randomly from the product produced in Korea. It was concluded that the sample size, the rate of seperation of a strip of the lining from the jaket and standard judgment should be more differed and divided in accordance with the fire hose number in adhesion test to get the high qualily of fire hose

운동, 흡연, 음주 습관이 운동부하검사성적에 미치는 영향

김희균,강인숙,황은주,배형준 임상병리검사과학회 1998 임상병리검사과학회지 Vol.30 No.3

Although there have been many studies on the risk factors for coronary artery disease, but there have been rare an attempt to examine the effect of risk factors on exercise test The purpose of this study was to research the effects of training, smoking and drinking habit on results of treadmill exercise test. The subject of study was adult patient 530 cases be questionable as coronary artery disease in Dept. of Cardiovascular Center, Kang Dong Sacred Heart Hospital between May 1997 to February 1998. In this study used to modified Bruce protocol(Case 16, Marquette co.). Total positive rate was 30.8%(144 cases) by treadmill exercise test, it was male higher than female(16.3%:14.6%), that was seen significantly differ as increase of age. As training habit, positive rate of nontraining group was higher than training group(18.4%:12.4%), have been no relationship both regularity and frequency between nontraing and traning groups, but performance degree of workload level was high in training group. As smoking habit, positive rate of smoking group was higher than nonsmoking group in males (57.9%:42.1%), 9 cases of total 20 cases in females, there was have been high positive rate(45%). As drinking habit, positive rate of between drinking and nondrinking groups was no significantly differ, but positive rate of drinking group was a few higher than nondrinking group in male and female groups.

SMEDDS를 이용한 난용성 약물의 용출율 향상

김계현,이윤석,배준호,지상철,박은석 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

ABSTRACT-A self-microemulsifying drug delivery system (SMEDDS) was developed to enhance the solubility and dissolution rate of poorly water soluble drug, biphenyl dimethyl dicarboxylate, DDB. The system was optimized by evaluating the solubility of DDB and the microemulsion existence range after the preparation of microemulsions with varying compositions of triacetin and surfactant-cosurfactant mixtures (Labrasol as surfactant (S) and the combination of Transcutol, Cremophor RH 40 and Plurol oleique as cosurfactant (CoS)). SMEDDS in this study markedly improved the solubility of DDB in water up to 10 mg/ml and the size of the o/w microemulsion droplets measured by dynamic light scattering showed a narrow monodisperse size distribution with an average diameter less than 50nm. The microemulsion existing range is increased proportional to the ration of S/CoS, however, it decreased remarkably as the oil content was more than 20%. In vitro dissolution study of SMEDDS showed a significantly increased dissolution rate of DDB in water (>12 fold over DDB powder), and SMEDDS also had significantly greater permeability of DDB in Caco-2 cell compared to powders.