DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

공무원들의 인식에 나타난 경관자원 특성 분석 연구

박선명(Park Sun-Myung),박선경(Park Sun-Kyung),김주현(Kim Joo-Hyun),김종하(Kim Jong-Ha),이정호(Lee Jung-Ho),최무혁(Choi Moo-Hyuck) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.11

In this study, to set up landscaping plans, characteristics of landscape were extracted based on opportunity and weaknesses factors of landscape after carrying out a questionnaire targeting officers in 23 cities and counties in Kyung-buk province and the findings are as following. First, in analyzing condition, component and value of landscape based on opportunity and weaknesses factors, for opportunity factors, it was found that the condition of landscape was quantitatively great, the component of landscape was industrially great and the value of landscape was great in possibility of development. Also, for weaknesses factors, the 'quality' was superior in its condition, the industry was superior in its 'component' and the shortage of artificial resources was superior in its 'value'. Secondly, in the relation between factors consisting opportunity and weaknesses resources, firstly, it was found that industry, city and nature are superior for opportunity factors, and culture, traffic and population are superior for weaknesses factors. Next, in the relation between component and condition, 'qualitatively superior industry' and 'quantitatively superior industry' had the highest frequencies in opportunity factors and 'qualitatively outstanding city' and 'quantitatively preponderant industry' had the highest frequencies in weaknesses factors. Thirdly, in analyzing the importance of opportunity and weaknesses factors, for opportunity factors, qualitatively superior resource was a priority in the importance of condition and for weaknesses factors, it was qualitatively little greater than the quantity. In the importance of component, the industry was most superior in both of strength and weak points and in the importance of value, the shortage of artificial resources was a priority. Fourthly, in characteristics of landscape, internal characteristics were found stronger than external characteristics in each city and county. This means that there are more resources to strategize in landscape plans of individual city and county unit than other in metropolitan unit. Also, possibility of development, possession of complex resources and geographical adjoin were common points of their strategies and plans.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

급성기 뇌경색 환자에서 화열 관련 증상과 증후의 변화에 관한 연구

곽승혁,박수경,우수경,이은찬,박주영,정우상,문상관,조기호,조승연,박성욱,고창남,Kwak, Seung-hyuk,Park, Su-kyung,Woo, Su-kyung,Lee, Eun-chan,Park, Joo-young,Jung, Woo-sang,Moon, Sang-kwan,Cho, Ki-ho,Cho, Seung-yeon,Park, Sung-wook,Ko, Ch 대한중풍순환신경학회 2011 대한중풍.순환신경학회지 Vol.12 No.1

Objective : Fire and heat related symptoms and signs are considered common in acute stage of diseases. The purpose of this study is to evaluate the occurrences and changes of fire and heat related symptoms and signs in acute cerebral infarction patients. Method & subjects : 40 acute cerebral infarction patients hospitalized in Oriental medicine hospital, Kyung-Hee University, who had examined and diagnosed 2 or 3 times based on oriental medical diagnosis were selected. We chose 23 as fire and heat related symptoms and signs from 94 diagnostic articles, and we added all those scores together of each patient. We analysed the scores of fire and heat related symptoms and signs as the time passed, and depending on oriental medical diagnosis. Result : In acute cerebral infarction patients of this study, 4 of fire and heat related symptoms and signs were took 1st, 2nd, 4th and 8th places in most changeable 10 articles of total 94 articles. The mean score of fire and heat related symptoms and signs of all patients were decreased significantly over the 3 times of measurements. The 8 patients diagnosed as fire and heat diagnosis at visit1 were samely diagnosed as fire and heat diagnosis at visit2, and at visit3 5 patients of them except for 3 patients excluded between visit2 and visit3, were still diagnosed as fire and heat diagnosis. At all of 3 measuring times, the scores of fire and heat related symptoms and signs of fire and heat diagnosis group were higher than non-fire and heat diagnosis group. Conclusion : This study indicated that fire and heat related symptoms and signs were very changeable phenomenon in acute cerebral infarction patients. And they decreased as time goes on.

호흡 부전을 동반한 급성 자율-감각신경병증 1예

박경필,안규환,김은주,김대성,정대수,박규현,김매자 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

시멘트 콘크리트의 건조수축균열 특성

박제선,윤경구,이주형,김태환,정원경 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

콘크리트는 본질적으로 인장강고와 휨강도가 약하며 취성적 성질을 나타내는 결점을 가지고 있으므로 이를 보강할 목적으로 콘크리트에 섬유를 혼입한 섬유보강 콘크리트가 크게 각광받고 있다. 특히 폴리프로필렌 섬유보강은 모르타르 및 콘크리트가 건조나 동해에 의해 수축될 때 구속에 의해 발생되는 인장응력 및 균열을 제어하고 인성의 증가와 충격, 마모, 피로에 대한 저항성, 내구성을 증대시키는 장점을 가지는 것으로 보고 되고 있다. 1축으로 구속된 건조수축의 구속으로 인한 조강형 콘크리트의 잔류응력을 선형모델을 이용하여 예측할 수 있었고 조강형 콘크리트에 섬유보강으로 인한 건조수축 제어효과는 일반큰코리트에 비해 효과가 매우 큰 것으로 나타났다. 따라서 폴리크로필렌 섬유의 혼입으로 인해 콘크리트의 균열제어가 가능하며, 균열 폭이 큰 균열보다는 많은 미세 균열을 유도하는 것을 알 수 있었다. 섬유보강 조강형 큰크리트의 동결융해저항특성은 거의 동해의 손상을 받지 않으며 좋은 내구성을 유지하고 있는 것으로 나타났다. 이는 불규칙하게 배향된 섬유가 공극수의 이동을 효과적으로 제어함으로써 메트릭스 내의 팽창압으로 인한 파괴를 최대한 줄였기 때문이라고 보여진다. 표면손상과 박리저항에 매우 우수하며 저항성을 증진시키는 효과가 뛰어남을 확인할 수 있었다. 따라서, 내구적 측면의 유지, 보수에 있어서도 섬유보강은 그 효과가 큰 것으로 나타났다. To improve the brittle nature of concrete, discrete fibers can be adapted in concrete. Since polypropylene fiber has many advantages in many points on its chemical stability and good durability, its usages have been increased gradually. It has been reported than polypropylene fibers can not only control restrained tensional stresses and cracks, but also increase toughness, resistance to impact, corrosion, fatigue, and durability. Although the polypropylene fiber reinforced concretes with ordinary cement have been studied so much, those with very early strength concretes have not studied at all. Therefore, it is necessary to initiate and conduct the researches for the polypropylene fiber reinforced very early strength concretes which has enough workability and durability. This study of polypropylene fiber reinforced very early strength concrete focused on the investigation each material's effect into strength development with analysis of restrained shrinkage properties with one-dimensional concrete members. The results showed that more optimized mixture combinations could be selected in very early strength concrete by incorporating polypropylene fibers, keeping in same strength levels at urgent repair works. The formation of needle-shape ettringite helps the early strength development, specially at flexural and this enables the repaired concrete structures or pavements to be opened to traffic after 3 hours of concrete placement. A residual stress of early strength concrete caused by one-dimensional restrained drying shrinkage was predicted from linear model. the control of shrinkage crack by fiber reinforcement at early strength concrete was better than that of ordinary portland cement concrete because of bridging effect of fiber at early age and resulting of more hair cracks rather than fewer wider cracks. As a results, this dissertation clearly validated the fiber reinforcement on the strength development and durability of polypropylene fiber reinforced very early strength concrete, and characteristic of restrained shrinkage. The proposed optimized concrete mixtures might be satisfactory applied at the repair works in fields, because of its durability and crack control capacity against freeze-thaw and contraction at early age, respectively.

Interleukin-1β에 의한 사람 정상 코점막 상피세포의 분비기능 및 형태 변화

김경수,윤주헌,최진우,이정권,문형진,박준호,전희선 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.11

배양된 해마 조직 절편에서 Beta-Amyloid Peptide의 신경 독성 효과

박정선,조미선,정연주,이경은 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Alzheimer's disease(AD) is primarily characterized by neurofibrillary tangles, senile plaques, and neurodegeneration. The major component of senile plaques is the beta-amyloid peptide(A B), Which is considered to have a causal role in AD. However, the biological activities of AB in AD has not been clearly defined. In this study we have investigated the effects of AB 25-35 fragment to neurons using organotypic hippocampal slice culture system which maintained intact hippocampal synaptic circuit and anatomy. Hippocampal slice culture is prepared from rat pos-tnatal 10-old days and after 14 days culture, slices were treated with 10uM AB 25-35 fragment. Neuronal death was measured with propidium iodide(PI) uptake and NeuN, neuronal marker, staining. After treatment of AB 25-35 fragment for 3days or 7days on hippocampal slice culture, we observed the increased PI uptake and the decreased number of NeuN-stained neuron in CA1 region of hippocampal pyramidal layer or dentate gyrus. These results suggested that AB 25-35 fragment exerts the neurotoxicity in hippocampal slice culture.

음악요법이 위내시경 대상자의 검사전 불안에 미치는 효과

박경숙,나명주 성인간호학회 2003 성인간호학회지 Vol.15 No.2

This study examined the effect of music therapy on the pre-exam of gastroscopy clients. Method: The research period is from June 17 2002 to August 30 2002, and the subjects were 60 individuals, 30 for the experimental and 30 for the control group among adult clients. The data were analyzed by using the SPSS program. Result: The results of this study were as follows: The score of the experimental group was lower than the score of the control group for the situational anxiety (t=-12.56, p=0.000) and systolic blood pressure (t=-3.97, p=0.000) before the examination was supported. The vital sign was partially supported. There was no significant difference(t=-1.05, p=0.297) in rhe diastolic blood pressure (t=-1.05, p=0.297) and pulse rate(t=-1.30, p=0.199) before the examination of the experimental group and lower than the score of the control group. Conclusion: Music therapy could be useful in the nursing practice, and be utilized as a way of nursing intervention to reduce anxiety of the patients in specific clinical situations. Finally, it is recommended that further studies be conducted on this therapy by utilizing different musical selections.

집먼지 진드기에 감작된 천식 환자에서 면역치료의 효과

박경원,이경은,조영주 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.2

Immunotherapy has been considered as secondary strategy of IgE mediated allergic disease. It decreases delayed airway inflammation and blocks irreversible airway change but many controversies still exist despite of its remarkable effect. The purpose of this study was to estimate clinical effectiveness of immunotherapy in patients with asthma sensitized to house dust mite and to evaluate related factors. Thirty asthmatic patients visiting allergy clinic of Mock-Dong hospital of Ewha woman university enrolled from March, 1996 to February. All patients were received the D. farinae and D. pteronyssinus standardized extract by a immunotherapy(IT) schedule and we measured clinical parameters. 1) Symptom-medication score was significantly improved after treatment (8.9±0.29 ; before IT vs. 3.4±0.20 ; after IT, p<0.05). 2) Methacholine challenge test demonstrated significant improvement (PC20 0.78± 0.09 before IT vs. 1.l8±0.17 after I year of IT, p<0.05). After we divided patients into two groups, group I that showed more than twice increasing in PC20 after I year IT and group Ⅱ that did not. There was no significant difference between the groups in pre-treatment PC20s (8.75±2.75mg/ml in group Ⅰ vs. 7.08±1.84mg/ml in group Ⅱ, p<0.05). 3) Skin prick test demonstrated no significant change (wheel flare ratio in D. farinae 2.44±0.24 before IT vs. 2.11±0.26 after IT, in D. pteronyssinus 2.33±0.29 before IT vs. 2.22±0.22 after IT, p>0.05). 4) There were no significant correlations for any combination among these three parameters (symptomatic improvement, change of PC20 and disease duration of asthma). 5) In view of side effects, no fatal systemic reaction occurred during treatment period. Only one patient experienced injection-related dyspnea and urticaria that were easily controlled by medication. This study demonstrated that patients with asthma sensitized to house dust mite benefit from specific immunotherapy through symptomatic improvement and reduced nonspecific bronchial hyperreactivity. As for side reactions immunotherapy might be relatively safe if allergen is carefully administered a controlled and supervised environment.