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      • fPTEX-0.4/한글 LATEX-0.991의 PDFLATEX에서 과학기술 연구논문에 그림파일을 삽입하는 방법

        윤희중,김종희,한완수 목원대학교 자연과학연구소 2000 自然科學 硏究論文集 Vol.9 No.2

        이 논문은 fpT_EX-0.4/한글L^AT_EX-0.991을 설치하여 L^AT_EX-2ε에서 작성된 논문에 그림화일을 포함시키는 방법에 대하여 기술하였다. 목원대 자연과학 연구논문집이 제8권 2호부터 L^AT_EX기반으로 한 minsT_EX으로 출판됨으로서 미려하고 완벽한 수식편집 환경이 우수한 것은 확인되었으나, minsT_EX이 기본으로 채택한 KPST_EX98(L^AT_EX-2.09)에서 그림파일을 논문에 삽입하는 것은 거의 불가능하였었다. 그리하여 우리는 최신 발표된 fpT_eX-0.4(00/10/3)버전으로 시스템을 업그레이드하고 UHC폰트도 사용할 수 있게 한글L^AT_EX-0.991을 인스톨하여 강력하고 안정된 L^AT_EX-2ε환경을 구축함으로서 좀 더 미려한 문서를 작성할 수 있었다. 특히 여기에서는 Windows의 그림판(WMF), Grapher(GRF), Photoshop(EPS, JPG, PSD, GIF), Origin(OPJ), SmartDraw(SDR), Mathematica(EPS)그림파일들을 EPS로 변환하고 문서에 삽입하는 방법을 예시와 함께 자세히 설명하였다. 이 문서는 2000년 12월 27일 현재, L^AT_EX-2ε 기반의 fpT_EX-0.4/한글L^AT_EX-0.991에서 PDFL^AT_EX으로 작성되고 시험되었으며, 자연과학연구소 연구논문집 제9권 2호의 모든 논문은 이 시스템에 의하여 제작되었다. This article described how to include graphic files in the page of your L^AT_EXdocument. We showed that the L^AT_EXenvironment is excellent in typesetting layout, but has revealed the poor compatibility with graphic files in the L^AT_EXsystem of version 2.09. It has been upgraded the L^AT_EX2εsystem with new version of fpT_EX-0.4/한글L^AT_EX-0.991and have checked the compatibility with various graphic packages. We showed that the graphic files such as PS, EPS, PDF, JPG, GIF could be included in the L^AT_EX2εdocument more easily than previous version of L^AT_EXsystem. This article is produced in the fpT_EX-0.4/한글L^AT_EX-0.991system, also all the article of Journal of Mokwon Institute of Natural Science Vol 9, No.2 are produced successfully in this system.

      • 女子大學生의 總脂肪量에 關한 調査硏究

        尹宗浣 상명대학교 논문집 1983 상명대학교논문집 Vol.12 No.-

        This study measured and analysed the fat weight contained in the bodies of students at a women's college through the physical measurement and the mean skinfold methods, and a skinfold thickness measurement from March to May, 1983. The number of subjects in this study numbered 308-(eighteen-year-old students numbered 61, nineteen-year-old students numbered 49, twenty-year-old students numbered 98, twenty-one-year-old students numbered 50 and twenty-two-year old students numbered 50) The results of this study were as follows: (1) According to the results of physical measurements, the average height of the body was 158.28 ± 4.23cm, the average weight was 52.14± 5.25kg, the chest girth was 83.88±4.36cm, and the sitting height was 86.50± 3.45cm. (2) The mean skinfold thickness of checkfold, measured by the large skinfold caliper, was 9.51± 4.22mm, in the case of abdomen it was 20.06± 5.23mm, the waist was 18.89± 5.35mm, the glutefold was 21.57± 4.25mm, the front thighfold, was 18.89± 6.23mm, and the total average was 18.31± 5.67mm. (3) The quantity and the rate of the fat weight contained on the body were the following; the eighteen-year-old group was 12.88kg and 24.98%, the nineteen was 13.04 kg and 24.85%, the twenty was 13.33kg and 25.47%, the twenty-one was 12.77kg and 24.62%, and the twenty-two was 12.96kg and 24.66%, and the total average was 12.99kg and 24.91%.

      • 都市成人男子의 Sports 活動에 關한 調査硏究

        尹宗浣 상명대학교 논문집 1984 상명대학교논문집 Vol.13 No.-

        The purpose of the article is to delineate and analyse the priority of the physical exercise events and the conditions of exercise operation of the male adults who live in the Seoul Metropolitan area through the questionnaire survey. A random sample was drawn of parents of women's university students from May to December, 1983. 438 respondents were made the object of this analytical study. The collected data were analysed by obtaining percentage for each category of the questionaire using the electronic computer IBM 4341. The demographic and economic characteristics of respondents indicate as following;a} characteristics of age ·····the seventies are 6, the sixties 14, the fifties 111, the forties 113, and the thirties 194. b) the characteristics of academic career·····those who posses attainments equal to or higher than those of the university graduates are 252, those of the senior high school 139, those of the junior high school 32, and the elementary school 15. c) the characteristics of income·····who take monthly income 900,000 won and the above over are 29, from 700,000 to 900,000 won 24, from 500,000 to 700,000 won 90, from 300,000 to 500,000 won 180, and 300,000 won and the below 115. As the result of this study following conclusions are gained; 1) The item of the physical exercise being preferred by the urban male adults is Tennis being ranked the first, 32.4 percentage, Socker the second, 15.1 percentage, and the third Mountain climbing, 13.7 percentage. 2) Tennis is the top rank of which are the most practiced events, are chosen 32.4 percentage, and the second rank, Jogging, 21.7 percentage. 3) Three quarters of the total, 74.2 percentage have been started the regular exercise by themselves spontaneously, and 13.0 percentage at the urging of a friend. 4) 35.8 percentage of the total are used to practice the exercise once per a week, 22.8 percentage twice, and 18.7 percentage carry it out every day. 5) 44.9 percentage of the total are shared thirty minutes with every exercise time, and 43.6 percentage sixty minutes. 6) The most of the respondents replied that leading a regular life and scheduled exercise are good for the health, former 43.4 percentage and latter 31.7 percentage. 7) 35.4 percentage of the total used to spent their leisure for rest, and 25.8 percentage for seeing television.

      • RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

        박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

        TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

      • KCI등재
      • B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

        정태호,김성국,서화정,윤영국,임종완,차상호,Chung, Tae-Ho 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

        목적 : 사람의 태아와 성인의 간세포에 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함. 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역법으로 조사한 결과 태아의 간조직에서는 HBsAg양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

      • B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

        정태호,김성국,서화정,윤영국,임종완,차상호 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

        사람의 태아와 성인의 간세포에 대한 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV 와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소 면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역 법으로 조사한 결과 태아의 간조직에서는 HBsAg 양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for, adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

      • Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrin Glucanotransferase의 정제와 효소특성

        김병우,김광현,남수완,권현주,송승구,윤종원 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

        Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purified enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and 60℃, respectively. The enzyme was stable at 50℃ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the ^α-, ^β-, ^γ-CD ratio of 42 : 46 : 12. Amylopectin was the most suitable with 67% conversion to CD.

      • KCI등재후보

        하계 실내 및 실외환경의 공기 중 휘발성 유기화합물 농도 및 개인노출

        양원호,손부순,박종안,장봉기,박완모,김윤신,어수미,윤중섭,류인철 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

        Volatile organic compounds (VOCs) are present in essentially all natural and synthetic materials from petrol to flowers. In this study, indoor and outdoor VOCs concentrations of houses, offices and internet-cafes were measured and compared simultaneously with personal exposures of each 50 participants in Asan and Seoul, respectively. Also, factors that influence personal VOCs exposure were statistically analyzed using questionnaires in relation to house characteristics, time activities, and health effects. All VOCs concentrations were measured by OVM passive samplers (3M) and analyzed with GC/MS. Target pollutants among VOCs were Toluene, o-Xylene, m/p-Xylene, Ethylbenzene, MIBK, n-Octane, Styrene, Trichloroethylene, and 1,2-Dichlorobenzene. Indoor and outdoor VOCs concentrations measured in Seoul were significantly higher than those in Asan except Ethylbenzene. Residential indoor/outdoor (I/O) ratios for all target compounds ranged from 0.94 to 1.51 and I/O ratios of Asan were a little higher than those of Seoul. Relationship between personal VOCs exposure, and indoor and outdoor VOCs concentrations suggested that time-activity pattern could affect the high exposure to air pollutant. Factors that influence indoor VOCs level and personal exposure with regard to house characteristics in houses were building age, inside smoking and house type. In addition insecticide and cosmetics interestingly affected the VOCs personal exposure. Higher exposure to VOCs might be caused to be exciting increase and memory reduction, considering the relationship between measured VOCs concentrations and questionnaire (p<0.05).

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