Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

수평 매복된 상악 견치의 교정적 견인

최형준,이종은,이제호,이종갑 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.4

치아 매복은 구강점막이나 악골내에서 치아의 맹출이 중지된 상태를 의미하며, 제 3구대구치와 상악 견치에서 호발한다. 상악 견치의 매복은 주로 측절치가 상실되거나 왜소한 경우와 연관성이 높지만 치배의 위치 이상과 같이 유전적인 요소와도 관련될 수 있다. 매복치는 심미적, 기능적으로 여러 가지 문제점을 야기하므로 조기에 진단하여 치료하는 것이 중요하며, 매복치의 상태에 따라서 치료 방법을 적절하게 선택하여야 한다. 본 증례는 11세 여아에서 구개측으로 수평 매복된 상악 우측 견치의 치료를 보고한 것으로, 매복치는 외과적 수술을 동반한 교정적 견인 방법을 사용하여 기능적, 심미적으로 회복되었다. 견인 치료는 매복 견치의 맹출 공간을 확보하면서 이루어졌으며, 치료 종료 후 매복 견치는 치근 흡수와 같은 합병증 없이 적절한 부착치은을 확보하여 양호하게 위치되었다. Tooth impaction is defined as a cessation of the eruption of a tooth at the level of the oral mucosa or alveolar bone. Maxillary canines are the most frequently impacted teeth next to the third molar. Maxillary canine impaction is associated with congenital missing of lateral incisors, peg lateralis and genetic factors such as ectopic positioning of a tooth germ. The clinicians have an important role in early detectionof tooth impaction for prevention of esthetic and functional problems. There are specific methods to treat impacted tooth for different conditions. In this case, an 11-year-old girl with a horizontally impacted maxillary right canine in a palatal position was treated through orthodontic traction along with surgical button attachment procedure. On regaining of eruption space, canine traction was performed. At the completion of treatment, the canine was positioned fairly within the arch with proper keratinized gingiva and complications such as root resorption were not observed.

산업장 소음노출 실태에 관한 조사

최형일,정경훈,유현종 조선대학교 환경공해연구소 1999 環境公害硏究 Vol.15 No.2

This research conducted an analysis of instantaneous sound pressure levels by means of classifying 246 processes at 41 workplaces in 11 kinds of industries. This research aims reveal how much noise level of each enterprise are exposed to the environment. This study investigated the distribution of noise induced hearing loss and the instantaneous sound pressure level in the audible frequency band. It also investigated the exposure intensity and characteristic of noise frequency according to the classification of industries. A frequency analysis was conducted on the processes which exceeded 85 dB(A). In addition, it was analyzed that the result of special health checkups and survey the laborers knowledge about noise according to their general characteristics. The rate of wearing protectors was also survey and the following findings were obtained : 1. Among the 246 processes of survey objects, the processes which exceeded 90dB(A), which is the maximum permissible standard for noise exposure designated by the ministry of labor were, 55 places, which corresponded to 22.4%. 2. When the analysis on frequency characteristics of 120 processes which exceeded 85 dB(A) in surveyed industries was conducted, the processes at 20 places, 16.7% showed the highest sound pressure level in the high frequency band which represented higher than 2 kHz. 3. The laborers having opinions that they had noise induced hearing loss were 85, which was 10.6% of 802 laborers who were the survey objects. 4. As for the rate of noise induced hearing according to age and years of service of laborers, the rate of noise induced hearing loss appeared higher as their ages increased and their years of service increased. 5. As for the tendency of recognizing their working environment, 80% of the subjects stated that their working sites were noisy, 51.2% stated that they easily felt tired.

통신설비의 Pulse성 Nosie 현상 분석과 Noise 대책

崔寅浩,崔種和,金炯澈,李相寧,鄭日和,李大寧 慶熙大學校 1993 論文集 Vol.22 No.-

The use of electric-power-distribution circuits for communications is of continued and growing interest. Impedence, attenuation, and noise characterize any communication channel and are needed for design of digital communcation systems. The purpose of this paper is to present the method of Measurement and countermeasure of the temporal and spectral properties of the magnetic and electronic fields of radio noise that emanates from power lines.

아르곤 레이저를 이용한 광중합 수복재의 물리적 성질에 관한 연구

최형준,주상호,김성오,이종갑 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.2

The purpose of this study is to evaluate and compare the results of argon laser for 5 seconds, argon laser for 10 seconds, and visible light for 40 seconds photo-polyerization in compressive strength, microhardness, curing depth, temperature rising during polymerization, and polymerization shrinkage. Hybrid type composite resin(Z-100) and compomer (Dyract) were used to be compared. The compressive strength was measured by an Instron (1mm/min cross head speed) in 60 specimens and the microhardness of the surface was expressed by Vickers Hardness Number (VHN) in 30 specimens. The curing depth was evaluated comparing the different values of upeer and lower VHN according to irradiation time and thickness for the light sourece polymeriaztion in 60 specimens. the temperature rising during photopolymerization was observed by the temperature change with thermocouple sensitizer beneath 40 specimens at th argon laser for 10 seconds and visible light 40 seconds irradiation. the polymerization shinkage was evaluated by calculating the decrease of % volume by using a dilatometer in 30 specimens. The results were as follows : 1. In the case of compressive strength, the argon laser polymerization groups were higher than visible light group in Z-100 (p<0.05). In Dyract, the argon laser 5 seconds group did not show a significant difference with the visible light 40 seconds group. The argon laser 10 seconds group showed the markedly low value when compared with other g개ups (p<0.05) 2. In micriohardness, Z-100 was better than Dyract when comparing by VHNs(p<0.05) ; however, there was not a significant difference between two materials in the visible light 40 seconds group and the argon laser 10 seconds groups. 3. In the study of curing depth, Z-100 showed the consistent polymerization in argon laser irradiation because there was no difference in the VHN decrease according to the thickness change. Over the thickness control, the results did not show a significant difference between visible light and argon laser group in Z-100 ; however, in the case of Dyrct, the visible light 40 seconds group was better than the argon laser groups(p<0.05). 4. There was a significant difference between the two materials in temperature rising during polymerization (p<0.05), but not a significant difference between irradiation times. 5. There was not a significant difference between the two materials in polymerization shrink-age. The argon laser 5 seconds group was smaller than the other groups (p<0.05). It could be concluded that z-100 polymerization was recommended to use the argon laser for reduction of the irradiation time while Dyract was recommended to use the visible light polymerization.

상악 영구 중철지의 외상성 치근파절 : 증례보고 A CASE REPORT

최형준,곽지윤,이종갑,최병재 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

청소년기에는 두개악안면부위의 외상성 손상이 흔하다. 그러나 상아질, 백아질, 치수를 포함하는 치아파절로 정의되는 치근파절은 비교적 드물다. 치근파절에 대하여 취할 수 있는 적절한 조치는 파절편의 치관부위를 재위치시키고, 2∼3달간 레진강선 고정술을 시행하는 것이다. 치수과사나 치근흡수의 징후가 나타날때까지 근관치료를 보류해야 하는데 대부분의 증례에서 파절편의 치근부위는 그 생활력을 유지하기 때문이다. 본 증례의 10세 4개월된 남아는 상악영구중절치의 치근파절을 주소로 연세대학교 치과병원에 내원하엿으며, 그후 7년동안 동일부위에 2번의 반복적인 외상이 있었으나 파절편 치관부의 재위치, 레진 강선 고정술, 근관치료 시행 후 임상적으로 방사선학적으로 비교적 양호한 치료결과를 얻었기에 보고하는 바이다. Traumatic injuries in the young permanent dentition are common, but root fractures, defined as fractures in volving dentin, cementum and pulp, are relatively uncommon. Appropriate management of root fracture involves repositioning the coronal portion of the tooth fragment and firm immobilization with a splint for 2 to 3 month. Root canal treatment should not be initiated until the sign of necrosis or resorption are apparent because in most cases, the apical fragments maintain their vitality. The following case report describes a patient with root fractures injured three times over the period of 7 years. The results, clinically and radiographically, were acceptable, but term periodic evaluation is required.

추적조사를 중심으로 한 腰椎間板脫出症에 대한 蜈蚣藥鍼의 臨床的 硏究

최회강,소기숙,고강훈,박소영,김성남,이종덕,원경숙,문형철,김성철 大韓鍼灸學會 2003 대한침구의학회지 Vol.20 No.3

Objective : This study is performed for the purpose of examining the efficacy of the scolopendrid(Scolopendra morsitans L) which has been to low back pain as a folk remedy. Methods & Results : Seventy patints with H.I.V.D. treated with the scolopendrid aquacupuncture and examined 4 tests; D.I.T.I., physical test, Oswestry disability index, and self-conscious pain rate were studied. And we follow up after discharge, we evaluated the present state and recovery period. Or we evaluated by herniated type and duration of onset. There was 58~97% of efficacy rate in each tests. Moreover, statistically valuable change in all 7D.I.T.I. points and in disability index and in self-conscious pain rate was showed. Conclusion : The scolopendrid aquacupuncture is possible to be effective terating the H.I.V.D patients. so we suggest the possibility using this new remedy to the H.I.V.D. patients.

온도; pH, 염분 내성 및 충격이 개불, Urechis unicinctus 유생에 미치는 영향

최상덕,김호진,라성주,정형택,윤호섭,신종암,이원교 國立 麗水大學校 環境問題硏究所 1999 環境硏究論文集 Vol.2 No.-

The effect of different temperature, salinity and pH on survival rate of Urechis unicinctus's swimming embryo was examined to develop the method for the efficient seed production. In addition to, this study was designed to investigate the possibility of pollutant marker diagnosis of marine pollutions by the change in different temperature, salinity and pH. Treatment was carried out whit different temperature (5˚C, 10˚C, 17˚C, 20˚C, 25˚C,), salinity(0‰, 10‰, 20‰, 30‰, 34‰, 40‰) and pH(4, 6, 7.2, 8.2, 9.2, 10). All the swimming embryo of the temperature experiment was survived within all experiment section, but the others experiment were not. At the temperature-shock and tolerance experiment ranging from 5˚C to 25˚C the survival rate was occurred: 75%, 95%, 100%, 100%, 50%, at the ph-Shock experiment ranging from 4 to 10, the survival rate was occurred: 0%, 50%, 85%, 100%, 60%, 0%, at the pH-tolerance experiment ranging 4 to 10 the survival rate was occurred : 0%, 75% 90%, 100%, 80%, 0%, and at the salinity-shock experiment ranging from 0% to 40% the survival rate was occurred : 0% 10% 90% 95% 100% 0% , at the salinity-tolerance experiment ranging from 0% to 40% , the survival rate was occurred : 0% 0% 90% 100% 100% 0%. These results suggest, that the survival rate of larvae of Urechis unicinctus was the highest at 17˚C, 34%. pH8.2 and this Urechis unicinctus could be live at the zone of euryceious and zone of euryhalines