Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

미스트 분무 및 스프링클러 관수가 단동 플라스틱 하우스에 미치는 냉각효과

강정윤,최동호,임홍성,허종철 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.2

As crops recently can be cultivated all the year in greenhouses, we need to improve the environment in which crops are able to grow in the summer. Therefore, in this study, we quantitatively got the cooling effects of 4 single span plastic greenhouses in the following ways : by spraying mist or sprinkler, or by opening the side window. and with those variables, we simultaneously did experiments at 4 greenhouses under equivalent conditions to reduce the temperature of the greenhouses in the summer. The results of the experiments are as following : 1) The temperatures of the greenhouses were effectively reduced by both spraying mist and sprinkler. 2) When the mist is intermittently sprayed. the indoor temperature has decreased by 4 degrees centigrade, meanwhile, in the greenhouse to make experiments using sprinkler, the temperature of that has been reduced by 2 degrees centigrade. Therefore, we can see the cooling effects of smaller mist in size as more effective than those of sprinkler. 3) When the greenhouse is naturally ventilated by opening the side window, we have to apply another cooling system because the indoor temperature is higher than the outdoor one.

여성 요도에서 발생한 이행세포암종 1례

윤종현,문기혁,송윤섭,박영호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Primary urethral carcinoma in females is uncommon neoplasm that only 0.02% of all types of cancer in women. Understanding the feature and character of disease has helped us select proper treatment in individual case. We report a case of primary transitional cell carcinoma of the female urethra with brief review of the literature.

지연유합과 불유합에서 저신호 강도 초음파의 유용성

윤여헌,김종오,고영도,유재두,정준모,오종건,방한천,최창호,신명철 대한골절학회 2003 대한골절학회지 Vol.16 No.1

목 적 : 저 신호 강도 초음파를 이용한 지연유합과 불유합의 치료에 대한 유용성을 알아보고자 하였다. 대상 및 방법 : 2001년 7월부터 2002년 5월까지 본원에 내원한 지연유합 7례와 불유합 8례를 대상으로 5개월간 저 신호 강도 초음파로 치료하여 5개월후 골유합 여부를 알아 보았다. 결 과 : 총 15례 중 대퇴골 간부 2례, 경골 간부 1례, 상완골 간부 1례, 요골 1례의 지연유합에서 골유합을 얻었고 대퇴골 간부 불유합 3례에서 유합을 얻었다. 지연주합은 71%의 유합율을, 불유합은 37.5%의 유합율을 보였다. 결 론 : 저 신호 강도 초음파는 골유합을 촉진 시킬 수 있으며 지연유합에서 시도해 볼 만 하나 불유합 치료를 위해서는 보다 많은 연구가 필요 하다. Purpose : To evaluation of usefulness of low-intensity ultrasound for nonunion and delayed union. Materials and Methods : For 5 months, we treated 7 delayed union and 8 nonunion using low-intensity ultrasound. After 5 months, in checked X-ray AP and Lateral view, when cortical bridge formation was done, we through union. Results : In 7 delayed union, 5 cases-2 femur, tibia, humerus, radius were healed. In 8 nonunion, 3 femur nonunion were healed. Union rate was 71% in delayed union 37.5% in nonunion. Conclusion : we thought that the low-intensity ultrasound has capacity of induction of union and was considered as the method of treatment for delayed union.

화훼작물의 여름철 플러그묘 생산에 있어 급격한 엽온저하 쇼크를 이용한 도장억제 가능성 검토

윤재길,박소현,강호종 진주산업대학교 2000 論文集 Vol.39 No.-

One of the most important problem during summer plug plant production is the succulent growth. The possibility of controlling height of plug seedlings by temperature drop shock(TDS) was investigated in Pharbitis, Impatiens and Callistephus. The most sensitive plant to TDS was Pharbitis. By 2℃ water irrigation, height of Pharbitis decreased by 33%. Impatiens showed 16% reduction in height by 2℃ water irrigation. Callistephus showed no reduction in height by any treamtnet. In all plants, stem diameter increased about 10% by the cold water irrigation. Leaf injury such as leaf spot of Saintpaulia was not observed. These results suggested that TDS could be used for controlling height of summer plug seedlings such Pharbitis which is sensitive to TDS.

시상출혈 환자의 지연 순목반사

박종무,김호진,윤창호,이용석,노재규,이광우 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

케미컬오일을 이용한 ERF 연마 특성

윤종호,이재종,이응숙,이동주,김영호 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

Electro-rheological fluid is recently used for the micro polishing of 3-dimensional micro-aspherical lens. It's also used for polishing small area defects on the wide flat wafer. Since ER fluid shows a behavior of viscosity changing under certain electric fields, micro polishing efficiency may be enhanced for certain cases. In this paper, a perfluorinated carbonyl fluoride oil based ER fluids was used to improve surface polishing rate and submicron-scale accuracy. As the polishing electrodes, micro size cylindrical tools had been used for maximizing the electric field. An experimental device, which was applied for micro polishing a number of wafers of 4 inches in size and other workpieces, was made on a precision polishing system. It consisted of a steel electrode, a wafer fixture, 10mA current and DC 5kV power supply unit, and a controller unit. From the Experiments, the ER fluid is applicable for micro polishing of small parts.

전자상거래에 의한 무역기업의 수출활성화 방안 : 부산지역의 무역 및 제조업체를 중심으로 Focused on Trade Firms and Manufacturers in Busan

윤광운,하종욱,김철호 국제무역학회 2003 국제무역연구 Vol.9 No.1

본 연구는 부산지역 무역기업들의 전자상거래 활용실태 및 활용에 따른 장애요인을 분석하고, 이를 통하여 부산지역 무역기업들의 전자무역에 의한 수출활성화를 위한 방안을 모색하였는데, 실태분석결과는 다음과 같다. 부산지역무역기업들이 정보통신수단을 얼마나 업무에 이용하고 있는가를 조사하고, 이용과정에서 직면한 문제점을 분석함으로써 향후 전자무역에 의한 수출활성화방안을 모색하였다. 첫째, 많은 기업들이 해외시장조사, 해외 거래선 발굴, 해외 광고마케팅 등 해외시장에 대한 접근과 관련한 분야에서는 전자상거래 및 전자무역을 많이 활용하고 있는 것으로 나타났다. 둘째, 전자상거래 및 전자무역을 활용함으로써 최대성과를 얻고 있는 업무영역으로는 해외시장정보수집, 자사 및 제품소개, 수출입관련 관리비용절감, 상담시간단축, 수출입관련 업무시간단축, 해외거래처발굴, 수출입관련 인건비절감 등으로 나타났다. 셋째, 전자무역활용에 따른 애로사항이 큰 무역거래단계로는 해외시장조사, 해외마케팅단계, 해외거래선발굴, 신용조회, 대금결제, 물류·운송 등 무역거래의 전반에 걸쳐 나타나고 있다. 따라서 전자무역을 통한 수출활성화를 위해서는 첫째, 전자무역활성화를 위한 정부의 체계적인 정책수립 및 지원이 요구되며, 둘째, 전자무역관련 전문인력을 양성하여야 하며, 셋째, 전자무역거래알선사이트를 활성화시킬 필요가 있으며, 넷째, 글로벌무역네트워크 및 전자거래인증기관의 구축이 요구된다. 그리고 다섯째, 전자무역관련 시스템간 호환성 및 보안성확보가 요구된다. The purpose of this thesis is to examine the practical uses of Electronic Commerce and hardships of trading companies in Busan, and in doing so, propose several suggestions to improve their exports by using EC. According to this survey, many trading companies aggressively make use of EC in the field of abroad market survey, advertisement but make little use of EC in credit inquiry, offer and acceptance, formation of contract, payment, claims and so on. The greatest result to get appling Electronic Trade were abroad market survey, abroad advertisement and marketing, and reducing the export-import related time and charges and so on. And in implementing Electronic Trade, there are some obstacles as follows; a shortage of a specialist in performing Electronic Trade, insufficient of policy support and reliable payment system, risk of individual information leakage, and lack of reliable certificate authority. Therefore, in order to facilitate Electronic trade, it is required to improve various international laws and regulations, promote the trade leads sites, establish global certificate authority, global electronic payment systems and global electronic trade network, and develop training expert programing related to Electronic Trade.

Cytokinin류의 경엽처리가 봄감자 동화물질 전류에 미치는 영향

蔡潤錫,姜鎬宗 진주산업대학교 2001 論文集 Vol.40 No.-

Effect of Cytokinins(Kinetin and Benzyl adenin :BA) on the yields and carbohydrats and nitrogen compounds in the different parts of the spring potatoes as Stadies. Both kinetin and BA caused sisnifican (P <0.05) increare in yields, (carbohydrats and nitrogen compounds in the plant) while no sisnificar increares were observed in treatments the average tubes weights per plant were increased incread in low concentration of kinetin treatment where are in hight concentration of BA. Starch conteints in leaf treated with kinetin and BA showed lower than that of the control, and in stem 1∼2% higher than that of the control the starch con in treat the control. The starch sugar contents in the tuber treated with kinetins and BA has no significan difference between the treatment and the control. The free sugar contents in the leaf, freated with kinetins showed me much significan differentes between the treatment and the control.

定植方法이 딸기의 發根및 生育에 미치는 影響

姜鎬宗,朴相眩,蔡潤錫 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

In order to improve the method of raising seedling and planting in strawberry "jang hee", effect of pot shape and planting method upon the growth of the plants were investigated. The results were summarized as followings; 1. In general, root growth was better at round pot and I pot(conventional pot) than at other pots. Number of root was 26, the highest at round pot, and followed by 20 at I pot. Maximu root length was 29.4cm at round pot, longer than 26.5 at I pot. Root dry weight was 3.72g at I pot, and followed by 2.87g at round pot. 2. When the strawberry seedling adhering bed soil were planted uprightly, root number and root dry weigth were 17.7 and 1.63g respectively, a little higher than 17.0 and 1.45g at conventional method. However, maximum root length was 28cm at the conventional method, longer than 20cm at planting uprightly with bed soil. 3. Leaf area was 62.1㎠ at bed soil adhered and 46.2㎠ at bed soil removed. Petiol length was 1cm longer at bed soil adhered than at bed soil removed. Root dry weight was 4.7g at bed soil adhered and 1.6g at bed soil removed.