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신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교
김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2
Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.
Rapid and efficient construction of markerless deletions in the <i>Escherichia coli</i> genome
Yu, Byung Jo,Kang, Kui Hyeon,Lee, Jun Hyoung,Sung, Bong Hyun,Kim, Mi Sun,Kim, Sun Chang Oxford University Press 2008 Nucleic acids research Vol.36 No.14
<P>We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the <I>Escherichia coli</I> genome via markerless deletion of selected genomic regions. The deletion process was mediated by a special plasmid, pREDI, which carries two independent inducible promoters: (i) an arabinose-inducible promoter that drives expression of λ-Red recombination proteins, which carry out the replacement of a target genomic region with a marker-containing linear DNA cassette, and (ii) a rhamnose-inducible promoter that drives expression of I-SceI endonuclease, which stimulates deletion of the introduced marker by double-strand breakage-mediated intramolecular recombination. This genomic deletion was performed successively with only one plasmid, pREDI, simply by changing the carbon source in the bacterial growth medium from arabinose to rhamnose. The efficiencies of targeted region replacement and deletion of the inserted linear DNA cassette were nearly 70 and 100%, respectively. This rapid and efficient procedure can be adapted for use in generating a variety of genome modifications.</P>
<i>sucAB</i>and<i>sucCD</i>are mutually essential genes in<i>Escherichia coli</i>
Yu, Byung Jo,Sung, Bong Hyun,Lee, Ju Young,Son, Sung Hwa,Kim, Mi Sun,Kim, Sun Chang Oxford University Press 2006 FEMS microbiology letters Vol.254 No.2
<P>sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD, sucAB, and sucCD under the control of a P(BAD) in E. coli MG1655, E. coli MG1655 (DeltasucCD), and E. coli MG1655 (DeltasucAB), respectively. These strains grew well in Luria-Bertani medium containing 0.1% arabinose, but not in the absence of arabinose unless the medium was supplemented with succinyl-CoA. Our results indicate that either sucAB or sucCD is enough to produce succinyl-CoA that is essential for cell viability.</P>
Han Ying-Hao,Mao Ying-Ying,Yu Nan-Nan,Jin Mei-Hua,Jin Ying-Hua,Wang Ai-Guo,Zhang Yong-Qing,Shen Gui-Nan,Cui Yu-Dong,Yu Li-Yun,Lee Dong-Seok,Jo Yu-Jin,Sun Hu-Nan,Kwon Jeongwoo,권태호 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3
In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII+/+ (WT) and PrxII−/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII−/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII−/− vs. PrxII+/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.
Jo, Tae Geun,Na, Yu Jeong,Lee, Jae Jun,Lee, Myoung Mi,Lee, Sun Young,Kim, Cheal The Royal Society of Chemistry 2015 NEW JOURNAL OF CHEMISTRY Vol.39 No.4
<P>A new and simple colorimetric receptor <B>1</B>, based on 2,3-diaminomaleonitrile and julolidine moieties, has been synthesized and characterized. <B>1</B> showed a selective colorimetric sensing ability for copper(<SMALL>II</SMALL>) ions by changing color from yellow to colorless, and could be utilized to monitor Cu<SUP>2+</SUP> over a wide pH range of 4–12. The detection limit of <B>1</B> (2.1 μM) for Cu<SUP>2+</SUP> ions is much lower than that recommended by WHO in drinking water (30 μM). Moreover, the receptor <B>1</B> can also detect fluoride by color change from yellow to orange, distinguishing the fluoride ions effectively from anions such as CH<SUB>3</SUB>COO<SUP>−</SUP> and CN<SUP>−</SUP>. It was also found that the <B>1</B>–F<SUP>−</SUP> complex was reversibly bound and could be simply reverted back through treatment with a proper reagent such as HCl. The sensing mechanism for F<SUP>−</SUP> was theoretically supported by DFT and TD-DFT calculations.</P> <P>Graphic Abstract</P><P>A chemosensor showed colorimetric sensing for copper(<SMALL>II</SMALL>) and fluoride by changing color from yellow to colorless and to orange. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5nj00125k'> </P>
Combining Neuroinformatics Databases for Multi-Level Analysis of Brain Disorders
Yu, Ha Sun,Bang, Joon,Jo, Yousang,Lee, Doheon Korean Society for Bioinformatics 2012 Interdisciplinary Bio Central (IBC) Vol.4 No.3
With the development of many methods of studying the brain, the field of neuroscience has generated large amounts of information obtained from various techniques: imaging techniques, electrophysiological techniques, techniques for analyzing brain connectivity, techniques for getting molecular information of the brain, etc. A plenty of neuroinformatics databases have been made for storing and sharing this useful information and those databases can be publicly accessed by researchers as needed. However, since there are too many neuroinformatics databases, it is difficult to find the appropriate database depending on the needs of researcher. Moreover, many researchers in neuroscience fields are unfamiliar with using neuroinformatics databases for their studies because data is too diverse for neuroscientists to handle this and there is little precedent for using neuroinformatics databases for their research. Therefore, in this article, we review databases in the field of neuroscience according to both their methods for obtaining data and their objectives to help researchers to use databases properly. We also introduce major neuroinformatics databases for each type of information. In addition, to show examples of novel uses of neuroinformatics databases, we represent several studies that combine neuroinformatics databases of different information types and discover new findings. Finally, we conclude our paper with the discussion of potential applications of neuroinformatics databases.