加工食品의 問題點

申光淳 대한의학협회 1990 대한의학협회지 Vol.33 No.1

최근의 백신 연구 동향

신광순,김철중 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-

苛性소다 處理에 依한 農産副産物의 飼料價値 增進硏究

辛炯泰,韓泳根,李光淳 成均館大學校 1982 論文集 Vol.31 No.-

A factorial experiment (6x5) involving 6 kinds of crop residues (barley straw, corn stover, rice straw, rye straw, soybean straw, wheat straw), 5 levels of sodium hydroxide (0,3,6,9,12%) was conducted with four Korean native male goats using a practical dairy cattle ration. Crop residues digestion was measured in situ with nylon bags containing test material in the rumen of the fistulated goats. Bags of 9×15㎠ made of 100-mesh nylon cloth were filled with one of ground crop residues to give 3-5g dry matter. These bags were put into the ventral sac of the rumen and taken out at the end of 72 hours. They were washed in cold water, dried at 105℃ for 8 hours and weighed. The straw dry matter remaining was calculated by difference and analyzed for organic matter. The results which were obtained with this experiment were as follows: 1. The nylon bag dry matter digestibility (NBDMD, %) and nylon bag organic matter digestibility (MBOMD, %) of NaOH-treated crop residues were highly significant different (P<0.01) in different levels of NaOH and kinds of crop residues. 2. The relationship between cell-wall constituents (Y, %) of crop residues and level of NaOH (X, %) was: Y=-1.63X+77.06 (r=0.99^**) 3. The optimum level of NaOH for improving NBDMD (%) from crop residues, on the basis of Shin et al (1981), was 0% NaOH for soybean straw, 3% NaOH for barley straw and wheat straw, 6% NaOH for corn stover and rice straw, and 12% NaOH for rye straw.

C형 간염 바이러스 core 단백의 대장균내 발현

신현진,김철중,신광순 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

C형 간염 바이러스(HCV)의 구조 유존자중 core 유전자를 fusion protein vector인 pGEX-2T vector에 cloning하여 core단백질을 발현시켜서 항원성을 증가시키고자 하였다. HCV의 core gene을 PCR (polymerase chain reaction) 기법을 이용하여 증폭한 후, 약 0.57kb 정도의 증폭된 core gene을 TA cloning vector에 cloning 한 후, restriction exzyme BamH I으로 잘라, gel elution method로 elution하여 pGEX-2T vector의 BamH I site에 cloning 시켰다. Cloning된 plasmid를 E coli cell인 JM105에 transformation시킨 후, IPTG에의해 protein induction 하였다. pGEX-2T에서 발현된 HCV core 단백질은 SDSPAGE및 Western-blotting 분석 결과, 양성 C형 간염 환자의 serum및 monoclonal antibody와 특이적으로 반응하였다.

신원방우황청심원액의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

In order to investigate the pharmacological properties of New Wonbang Woohwangchungsimwon Liquid (NSCL), effects of Wonbang Woohwangchungsimwon Liquid (SCL) and NSCL were compared. In isolated rat aorta, NSCL and SCL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6M) regardless to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxing effect of NSCL and SCL. NSCL and SCL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NSCL and SCL significantly decreased heart rate. NSCL and SCL, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NSCL and SCL had no effects on parameters of action potential such as resting membrane potential, action potential amplitude, APD_90 and V_max at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NSCL and SCL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between cardiovascular effects of two preparations.

신우황청심원액의 심혈관계에 관한 약효연구

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), efects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^6M) without regard to intact endothelium or denuded rings of the rat aorta. Furthemore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dosedependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no efects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.

I-Muscone의 심혈관계에 관한 약리연구

조태순,김낙두,허인회,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

In order to investigate the pharmacological properties of l-muscone, effects of l-muscone and musk were studied on the cardiovascular system with various experimental models. In isolated rat aorta, l-muscone and musk made the relaxation of blood vessels in maximum contractile response to phenylephrin (10^-6M) in endothelium-containing rings of the rat aorta, but not in endothelium-denuded rings. However, l-muscone and musk in the presence of the inhibitor of NO synthase and guanylate cyclase did not make the relaxation of blood vessel. In spontaneously hypertensive rats (SHRs), l-muscone and musk slightly reduced blood pressure but significantly decreased heart rate. In the isolated perfused rat hearts. l-muscone and musk did not affect significantly on LVDP, contractile force, coronary flow and (-dp/dt)/(+dp/dt). These results suggest that l-muscone and musk have weak cardiovascular effects with relaxation of blood vessel and decrease of heart rate, but without significant cardiac functions.

韓國의 中學校 科學敎科書와 美國 SciencePlus의 生物領域 比較

박광순,신진범,김온식 공주대학교 사범대학 과학교육연구소 1997 과학교육연구 Vol.28 No.1

한국의 중학교 과학교과서와 미국 SciencePlus의 생물영역을 내용을 검토하고 상호 비교 분석한 결과 다음과 같다. 생물영역의 지면 수를 보면 한국 과학교과서는 265쪽인데 미국의 SciencePlus는 294쪽이었으나 SciencePlus는 그 내용이 탐구학습에 초점을 두고 많은 연습문제로 되어있다. SciencePlus는 많은 그림이 천연색으로 소개되어 생동감 있는 자료로 소개되었으며 그림을 보면 학습목표에 도달하도록 교도발견 식으로 편제되었고 탐구문제가 단원마다 그 앞에 8∼17항목이 있었다. 실험 수를 보면 한국 과학교과서는 60회 미국 SciencePlus는 33회로 되어 있었고. SciencePlus의 경우 한국 과학교과서 보다 실험 수는 줄이고 실험을 설명하는 그림이나 사진으로 대치하고 우수 학생을 위한 지도 자료가 교사용 지도서에 많이 제시되었다. 수준 강조도를 보면 한국 과학교과서는 개체, 조직과 기관 순으로 지면 수를 점유했으며 미국 SciencePlus에서는 생물과 환경 단원이 많은 지면 수로 점유했다. S.T.S 주제 영역에 따른 강조도의 지면 수를 비교하면 한국은 전체 지면의 6.3%의 점유로 ①기술 발달의 영향 ②환경 ③천연자원 순으로 구성되었으며 미국 SciencePlus는 전체 지면의 76.5%의 점유로 ①환경 ②천연자원 ③과학 사회학 순으로 구성되어 있다. We came to a conclusion after we compared the biology part of the Korean middle school science textbooks with that of SciencePlus in U.S.A. It is as follows. The biology part has 265 pages in the Korean science textbooks, while SciencePlus has 294 pages, and its content is focused on many explorations and composed of examples of brain teasers. In SciencePlus's case, a lot of color pictures are composed in a guided discovery way and they are showed vividly so that students may reach the learning points with ease and including 8 to 17 explorations. Experiments are presented 60 times in the Korean textbooks, while they are presented 33 times in SciencePlus. Though SciencePlus reduced number of experiment than the Korean textbooks, but it has many explorations, brain teasers, science in Actions, Science on your owns, pictures and photographes that can help students to understand well and teaching materials for superior students are shown very much in the guidance book for teachers. When we examine the focus from each level, ontogeny takes the largest, histology and organs are the next in the Korean textbooks, while histology and organs takes the largest, ontogeny is the next in SciencePlus. When we examine the focus of each subject, the structure and the function is emphasized in the Korean textbooks, while life and environment is emphasized in SciencePlus. When we examine pages of the focus according to the subject sphere of S.T.S, in the Korean textbooks the first is the influence of development of technology, the second is environment and the third natural resources (total 6.3%), while the first is environment, the next is natural resources and the third is sociology of science in SciencePlus (total 76.5%).

신원방우황청심원의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate the phamacological properties of New Wonbang Woohwangchungsimwon Pill (NSCH), effects of Wonbang Woohwangchungsimwon Pill (SCH) and NSCH were compared using various experimental models. In rat aorta, NSCH and SCH made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not aftect significantly the relaxing effects of NSCH and SCH, NSCH and SCH inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs). NSCH and SCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In guinea-pig papillary muscle, these had no effects on parameters of action potential such as action potential amplitude (APA). V_max and resting membrane potential (RMP) at low doses, whereas inhibited the cardiac contractility at high doses. Furthermore, these had a significant inhibitory effects on palpitation of the heart in normotensive rats and SHRs. These results suggest that NSCH and SCH have weak cardiovascular effects, and that there is no significant differences between cardiovascular effects of two preparations.

자연 교과 지도에 대한 초등 교사의 인식

김광명,신순임,강태완,이대형,장병기 춘천교육대학교 과학교육연구소 1995 과학교육연구 Vol.18 No.-