인공사육장에서 패혈증으로 집단폐사한 북방산개구리의 증례 보고

정여진 ( Yeo Jin Jeong ),김종택 ( Jong Taek Kim ),서국현 ( Guk Hyun Suh ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.3

Frog culture industry is not yet familiar but has much potential. Generally, in farm, the population density is higher than that of in nature and frog farm is not the exception. But when population density is high, it can easily leads to stressful condition, poor sanitation. When a disease occur, it is a primary factor that makes the population more susceptible and the results more grave. Because of severe Rhabditoidea- helminth infection and subsequent bacterial septicemia, 50¡­70% of the total population had been died in a farm in Jeong-sun in Gangwon-do and Chungju in Chungcheongbuk-do from late June, 2012 to September, 2012. Diseased frogs showed ruptured lung, bloody ascites, liver discoloration, myocardium weakness, congested kidney, microcytic anemia and so on. Enterobacteriacea, Citrobacter.sp, Cupriavidus metallidurans, Acinetobacter.sp were isolated as major bacterium that had caused septicemia in frogs. Among isolated bacterium, Cupriavidus metallidurans, Ewingella americana, Shewanella aquimarina and Pseudoalteromonas sp. have not reported as potential pathogens in frogs before. It is a good example that severe helminth infection in frogs can lead to secondary infection of bacteria.

경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

아크릴 부목을 이용한 하악골 골절의 새로운 치료법

정두성,박재현,김진환,최준 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

As modern society becomes increasingly complex, there has been a steady increase in violence, as well as in automobile and industrial accidents. This has resulted in an increased incidence of facial injuries, including mandibular fractures. Many methods have been advocated to achieve reestablishment of normal feature and function. The choice generally depends upon such factors as location of the fractures, displacements, status of the dentition and favorability of the fracture line. Many cases of mandibular fracture are treated by intermaxillay fixation using an arch bar or bite block(acrylic splint)for rapid bone union and minimal displacement. However, there had been some problems, such as discomfort to patients, limitation to eating and weight loss, poor oral hygiene, and temporomandibular joint ankylosis. As a consequence, we have modified the shape of the acrylic splint and method of fixation for circum-mandibular fixation instead of intermaxillary fixation. Acrylic splints are rigid, strong, easily adjusted and repaired, translucent, lightweight and well tolerated by the oral mucosa. The splints are properly secured to each tooth and bind all the teeth together as a single unit. Authors have used modified acrylic splints as tools of circum-mandibular fixation in 12 cases of mandibular symphysis and body fracture from May 1997 to August 1998, achieving the results of very good occlusal relationship, oral hygiene and comfort to patients.

金銀花 및 魚腥草가 人體 癌細胞柱에 미치는 影響

陳千植,崔政和,鄭鉉雨 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

국내 사망률 가장 높은 비율을 차지하고 있는 惡性腫瘍에 대하여 국내외적으로 다양하게 연구되고 있다. 그러나 아직까지도 腫瘍을 치료하기 위하여 手術治療·放射線治療·免疫治療·化學治療등의 많은 치료법을 개발하고, 또한 실험실 및 임상에서 많은 연구보고가 이루어지고 있지만 腫瘍의 치료원칙 및 치료약물에 대해서 미흡한 것이 국내의 현실이다. 일반적으로 抗癌劑를 이용한 化學治療등이 사용되고 있지만 이에 따른 副作用이 많아 본 연구자들은 抗癌劑와 韓藥材를 倂用投與하여 副作用을 최소화하고, 이에 따라 韓藥材가 正常細胞에 어떠한 영향을 미치고 , 癌腫細胞에는 어떠한 영향을 미치는가에 대하여 관찰한 결과 有意性이 있어 보고하게 되었다. 淸熱作用이 金銀花와 魚腥草를 인체의 皮膚癌細胞인 A431 細胞, 子宮癌細胞인 HeLa 細胞, 急性白血病細胞인 MOLT-4 細胞, 慢性骨髓性白血病細胞인 K562 細胞에 대한 細胞毒性과 抗癌劑인 mitomycin C와 倂用처리결과를 MTT assay를 통하여 관찰하였다. 또한 正常細胞에 대한 細胞毒性을 검색하기 위하여 마우스 섬유아세포(Balb/c3T3), 마우스 胸線 및 腥臟細胞, human lymphocyte에 미치는 細胞毒性을 검토하였다. 魚腥草는 慢性骨髓性白血病細胞柱에 직접적인 細胞毒性을 나타냈고, mitomycin C와 倂用 處理時 細胞毒性을 증가시켰으며, 마우스 섬유아細胞인 Balb/c 3T3 細胞의 增殖을 억제시켰다. 또한 金銀花는 마우스 胸線細胞의 增殖을 촉진시켰고, human lymphocyte의 增殖을 촉진시켰으며, 金銀花 및 魚腥草 모두는 마우스 腥臟細胞의 增殖을 촉진시켰다. The purpose of this research was to investigate effect of water extract of Flos Lonicerae and Herba Houttuyniae on the proliferation of human cancer cell-lines. The effects of Flos Lonicerae and Herba Houttyniae on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. 1. Flos Lonicerae and Herba Houttuyniae inhibited the proliferation of K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was decreased by the combination of Herba Houttuyniae. 3. Herba Houttuyniae inhibited the proliferation of Balb/c 3T3 cells. 4. Flos Lonicerae stimulated the proliferation of thymocytes. 5. Flos Lonicerae and Herba Houttuyniae stimulated the proliferation of splenocytes. 6. Flos Lonicerae stimulated the proliferation of human lymphocytes.

뇌동맥류 수술환자의 정신신경학적 분석

정현태,박순필,김상진,도종웅 대한신경외과학회 1987 Journal of Korean neurosurgical society Vol.16 No.4

The mortality rate of aneurysmal surgery had recently been reduced remarkably due to mordern microsurgical technique and refinement of the timing of operation. Despite recovery without neurological deficits, many patients remain with emotional and psychological sequelae postoperatively. We evaluated 33 patients, operated on intracranial aneurysm for recent 2 years, about quality of life and degree of cognitive function. For proper evaluation of outcome of aneurysmal surgery, in addition to the neurological outcome, psychological aspects must be considered.

방사선작업종사자에게 발생한 암의 업무관련성 평가를 위한 선별선량의 활용

정미선,진영우,임현술,김종순 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.3

위암조직에서 Carcinoembryonic Antigen 측정의 의의

정현용,윤세진,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

To estimate tumor CEA expression of stomach cancers in relation to normal mucosa CEA, degree of cellular differentiation, pathologic stage and other clinicopathologic findings, we measured CEA concentrations in tissue extracts by radioimmunoassay for 24 cases of stomach cancer. The results were as follows: 1. Ranges of tissue CEA concentrations in normal stomach mucosa was from 6.Ong/gm to 15.4 ng/gm(mean 10.2ng/gm). 2. Mean conceatrations of cancer tissue CEA was 42.1ng/gm(from 2.9 ng/gm to 414.Ong/gm), 4 times higher than normal mucosa. In 46% of stomach cancers, tissue CEA concentrations was above normal cut off value(18.7ng/gm). 3. Tiuse CEA concentrations of stomach cancer was significantly high as high CEA levels of normal mucosa(r=0.6954), not related to serum CEA concentrations. 4. Tissue CEA concentrations of stomach cancer was high as high TNM stage, but was not related to wall invasion depth, lymph node metastasis, cellular differentiation and other clinicopathologic findings. In conclusion, the production of CEA in stomach cancers was significantly increased in half cases, CEA expression of tumor was related to pathologic stage rather than cellular differentiation. The factors determined CEA production was considered more higher levels such as DNA profile. To estimate prognostic values of tissue CEA concentrations, follow up of included patients and further study should be pursued.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

탈력발작 유무에 따른 기면병 환자의 비교 : 임상변인, HLA-DQB1*0602, Hypocretin

정종현,홍승철,신윤경,한진희,이성필 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1

Objectives : Narcolepsy is a sleep disorder, characterized by excessive daytime sleepiness, cataplexy, sleep paralysis and hypnagogic hallucination. Among these symptoms, cataplexy is one of the most pathognomonic symptoms in narcolepsy. This study was designed to investigate the clinical features, frequency of DQB 1 *0602 and CSF hypocretin levels in Korean narcolep-tics with cataplexy to compare with those who have not cataplexy. Methods : From August 2003 to July 2005, we selected 72 patients who have narcolepsy confirmed by nocturnal Polysomno-graphy and multiple sleep latency test (MSLT) as well as their history and clinical symptoms at Sleep Disorders Clinic of St. Vincent's Hospital, Catholic University of Korea. Patients were divided into 56 cataplexy-positive group (narcolepsy with cataplexy group) and 12 cataplexy-negative group (narcolepsy without cataplexy group). HLA typing was done in all patients for the presence of DQB 1*0602, and patients received spinal tapping to measure the level of CSF hypocretin. Clinical variables were examined by semi-structured interview for narcolepsy patients. Results : 1) In cataplexy-positive group, compared with cataplexy-negative group, the frequency ofHLA-DQB 1*0602 was found to be significantly increased (50 subjects, 89.3% vs. 8 subjects, 50.0%) (p=0.000). 2) tn 48 out of 56 cataplexy-positive patients (85.7%), hypocretin levels were decreased (≤110 pg/mI) or were below the detection limit of assay (≤40 pg/ml).However, only 6 out of 16 cataplexy-negative patients (37.5%) exhibited decreased hyopcretin level. The difference between two groups were statistically significant (p=0.000). 3) Cataplexy-positive group, compared to cataplexy-negative group, re-ported more frequent hypnagogic hallucinations (36 subjects, 64.3% vs. 4 subjects, 25.0%) (p=0.005). However, there were no significant differences in frequency or seventy of daytime sleepiness, sleep paralysis and demographic data. 4. In nocturnal polysomnography and MSLT findings, there were no significant differences in all sleep parameters between two groups. Conclusion : Higher frequency of HLA-DQB 1*0602, and lower hypocretin levels in cataplexy-positive groups than Cata-pelxy-negatives suggest that narcoleptics with cataplexy might be a etiologically different disease entity from narcoleptics without cataplexy. Additionally, Current criteria prevail for the diagnosis of narcolepsy need to be reclassified according to the presence of cataplexy or not.

Modified Hogan 법을 이용한 범인두 부전 환자의 인두피판 성형술

진우정,정기범,이현상,고승오 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

A competent velopharyngeal sphincter is essential for intelligible speech. If the velopharyngeal incompetence exist, the seal will not be complete during speech, with a resultant hypernasal speech quality. The patient with velopharyngeal incompetence(VPI) may develope other compensatory speech problems. There are many approaches available to correct veolpharyngeal incompetence, which include speech therapy, push back palatorrhaphy, pharyngeal wall implants and pharyngoplasty. This is cases report of velopharyngeal incompetence, which were successfully treated by superiorly based pharyngeal flap, covered with splitted hinge flap of nasal lining mucosa of the soft palate, named modified Hogan method. The advantages of this method are precision in the approximation of the flap due to pentagonal shaped flap design, good flood supply due to omission of the midsagittal incision on nasal lining mucosa, and simplicity than Hogan method.