Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

양극성장애와 새로운 항경련제의 효과

남범우,서정석,정헌종,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-

Bipolar disorder, like epilepsy and migraine, is episodic in nature and psychiatric illness requiring lifelong treatment. So, it should not be surprising that anticonvulsants such as carbamazepine and valproate have proven efficacy as mood stabilizers. The newer anticonvulsants such as lamotrigine, gabapentin, topiramate, oxcarbazepine, tiagabine, and zonisamide may also be effective treatments for bipolar disorder. Identifying an anticonvulsant for use in bipolar disorder should take into account not only the pathophysiology of bipolar disorder but also the mechanism of action of the anticonvulsant. Evidence for effectiveness of these novel antiepileptic drugs in treating acute mania and depression as well as in preventing the recurrence of mania and depression is reviewed. In conclusion, although the novel anticonvulsants appear to be clinically useful, more controlled trials are needed to assess the effectiveness in bipolar disorder.

폐구리염화물용액의 분무열분해반응에 의한 생성분말의 특성에 관한 연구

박희범,최재권,한진아,유재근 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 연구의 목적은 폐 구리염화물 용액을 원료로 사용하여 분무열분해 공정에 의해 평균입도가 1 ㎛ 이하이며 입도분포가 균일하고 치밀한 조직을 나타내는 미립의 구리산화물 분말을 제조하는데 있다.또한 본 연구에서는 분무열분해 공정에 의해 생성되는 분말의 특성에 영향을 미치는 반응 온도, 원료용액의 유입속도, 분위기기체 및 공기의 유입속도, nozzle tip 크기 및 원료용액의 농도 등의 반응인자들의 영향을 검토하였다.

가용화 조성물과 난용성 약물군을 함유하는 고체분산체의 용출양상

김태완,최춘영,Cao, Qing-Ri,권경애,이범진 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.3

Polymer based physical mixtures or solid dispersions containing solubilizing compositions[OA, tween80 and SLS] were prepared using a spray-dryer. Lovastatin(LOS), simvastatin(SIMS), aceclofenac(AFC) and cisapride(CSP) were selected as poorly water-soluble drugs. Dextrin, poly(vinylalcohol)(PVA), poly(vinylpyrrolidone)(PVP) and polyethylene glycol(PEG) were chosen as solubilizing carriers for solid dispersions. The solid dispersions containing solubilizing compositions without drug were prepared without using organic solvents or tedious changes of formulation compositions. This system could be used to quickly screen the dissolution profiels of poorly water-soluble drugs by simply mixing with drugs thereafter. In case of solid dispersion containing drug, organic solvent systems could be used to solubilize model drugs. The dissolution rates of the drugs were higher when mixed with drug and solid dispersions containing solubilizing compositions. However, solid dispersions of LOS, AFC, CSP simultaneously containing drug and solubilizing compositions in organic solvent systems were more useful than physical mixtures of drug and solid dispersions without drug except SIMS. Based on solubilizing capability of polymer based physical mixtures in gelatin hard capsules, optimal solid dispersion system of poorly water-soluble drugs could be formulated. However, it should be noted that dissolution rate of poorly water-soluble drugs were highly dependent on drug properties, solubilizing compositions and polymeric carriers.

적색 석류석 보석의 보석·광물학적 특징

김금조,김진섭,김원사,최진범 한국광물학회 2003 광물과 암석 (J.Miner.Soc.Korea) Vol.16 No.1

보석으로 사용되고 있는 파이로프-알만딘 계열의 보석을 대상으로 하여 이들의 화학성분, 결정구조, 굴절율, 비중, 색, 광택 등의 광물학적 성질을 알아보았다. 또한 위 고용체의 광물학적 성질이 화학성분의 치환 정도에 따라 점이적인 변화 양상이 나타났는지에 대해서도 연구하였고, 발색소에 대한 연구를 실시하였다. 특별히 보석업계에서 관행적으로 분류 기준으로 삼아온 굴절율과 비중 값이 광물학적으로 오류가 없는 것인지를 검증하는 것 등도 주요 연구목적에 포함되었다. 적색 석류석 시료 중 화학성분은 따른 분류기준에 의하면 17개가 파이로프이며, 6개가 알만딘임을 알수 있다. 파이로프의 굴절율은 1.77까지이며, 알만딘은 그 이상임을 알 수 있으며, 비중은 파이로프인 경우 3.88까지의 범위이며, 파이로프는 적어도 4.11 이상임을 알 수 있었다. 따라서 보석업계에서 파이로프와 알만딘을 구분하여 온 굴절율 기준(R.1. 1.75)은 오류이며, 비중값 기준 (S.G. 3.88)은 무난한 것으로 밝혀졌다. 단결정 X-선회절분석 결과에 따르면, 파이로프-알만딘 석류석이 모두 등축정계의 Ia3d 공간군에 속하며, 화학성분의 변화에 따른 단위포의 크기는 변화를 느낄 정도의 차이는 없었다. 또한 굴절율과 비중은 파이로프-알만딘 고용체에 있어 특히 FeO 함량에 따라 일정한 변화양상을 나타내는데, FeO의 함량이 증가할수록 굴절율과 비중이 증가함을 알 수 있다. 화학분석 자료에 의하며, 적색 및 보라색은 Fe^(+2), 황색은 Mn^(+2) 함량에 따라 크게 좌우됨을 알 수 있다. 적색 석류석내에 들어있는 결정 내포물은 주로 저어콘과 금홍석이었다. Chemical composition, crystal structure, refractive index, specific gravity, color, and luster were studied for pyrope-almandine series garnets. The main coloring agents determining the reddish or brownish garnets were also investigated. It was also examined if there is any relationship between mineralogical properties with respect to the various chemical compositions in the solid solution, in the hope to figure out the existing classification values of R.I. and S.G. using gemtesting facilities to distinguish pyrope from almadine. It was found that 17 out of the 24 specimens belong to pyrope and the rest almandine. R.I. of pyrope goes up to 1.77 and that of almandine is higher than the value. S.G. of pyrope reaches to 3.88 and that of almandine is greater than the value of pyrope. X-ray diffraction data revealed that pyrope-almandine garnets are isometric with space group Ia3d , and also show that the variation of cell parameters are not significant enough to parallel with the chemical compositions of the series. R.I. and S.G. increase with FeO content. Fe and Mn are most responsible to the red-purple and orange coloration of the specimens, respectively. Both zircon and rutile crystals are most common inclusions in the reddish stones.

가용화 조성물을 함유한 PVP형 고체분산체의 제조 및 특성 : Based Solid Dispersion Systems Containing Solubilizers

Cao, Qing-Ri,김태완,최춘영,권경애,이범진 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.1

The PVP-based solid dispersion systems (SDs) containing lovastatin (LOS) and solubilizers (sodium lauryl sulfate, tween 80 and oleic acid) were prepared to enhance dissolution rate of practically water insoluble LOS using solvent evaporation method. Two different organic cosolvents either acetone/ethanol or acetonitrile/ethanol were used for the preparation of SDs. The LOS contents were highly decreased when acetone/ethanol cosolvents were used. The decrease of LOS contents was not caused by acetonitrile or acetone, based on HPLC data. The surface morphology as investigated by scanning electron microscope (SEM) and angle of repose as an index of flowability of SDs were highly dependent of the type and amount of solubilizers used. Base on differential scanning calorimetry (DSC) and X-ray powder diffraction data, the SDs made crystalline LOS into amorphous structure or partially eutectic mixtures. The simultaneous use of the solubilizers in SDs was also useful to increase dissolution rate of LOS in gastric or intestinal fluid. The SDs containing solubilizers reached 76% and 60% in gastric and intestinal fluid, respectively but the commercial tablet gave only less that 4%. These solubilizers in SDs could be also applicable for enhancing dissolution and bioavailability of poorly water-soluble drugs.

캡사이신유도체를 함유하는 폴록사머 겔제제의 물리화학적 특성 : based Gel Containing Capsaicin Analog

김태완,조청일,최춘영,이범진 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.1

Physicochemical changes of poloxamer-based gel containing capsaicin analog (N-[3-(3,4-dimethylphenyl)propyl]-4-(2-aminoethoxy)-3-methoxypheny] acetamide) such as drug content, viscosity and surface tension were investigated during the storage conditions at three different temperatures (25, 40 and 60℃) over 90 days. No noticeable changes of color were observed when stored at 25 and 40℃. However, the color of white poloxamer gels turned yellow during storage at 60℃. The drug contents were unchanged during storage at 25℃ but had tendency to decrease at 40℃. The drug contents were highly decreased over 40-50% when stored at 60℃. The viscosity of a poloxamer-based gel was unchanged during storage at 25 and 40℃ but greatly increased at 60℃. The surface tension of a poloxamer-based gel was not changed at three different temperatures. The storage conditions of a poloxamer-based gel containing capsaicin analog can be considered for further clinical applications.

암면재배 수박의 품종별 당함량 비교

박순기,이범선,최진호,김월수,정순주 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

The fruit qualities and sugar contents of hydroponically grown watermelon cultivars were investigated in order to obtain informations for nutriculture of watermelon in glass house. The fruit circumference of Mudeungsan watermelon was increased from 30 to 38 days after pollination, and periods of fruit development was longer than that of other cultivars. Total soluble solid content in fruit flesh of watermelon was high in Samboggool, Gwigongja, Dalgona, Gamro, Mudeungsan, Olimpia, Hwangto and Geumro in order. Maltose content was higher in Gwigongja and Mudeungsan watermelon cultivar Sucrose content was higher in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Glucose content was much higher in Mudeungsan watermelon cultivar while very lower in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Fructose content was higher in Hwangto, Gamro, and Mudeungsan watermelon cultivar while was lower in Gwigongja watermelon cultivar than that of other watermelon cultivars. Maltose, glucose, and fructose content in fruit fresh of watermelon was higher in Mudeungsan watermelon than that of other watermelon cultivars. Contents of maltose, fructose and glucose in fruit flesh of watermelon were higher in Mudeungsan watermelon than those of other cultivars.

현무암 석분 활용에 대한 연구

민경원,진호일,최성범,박진동 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

철원지역에서 발생되는 현무암 석분은 주로 휘석과 사장석, 방해석, K-장석 등으로 구성되어 있고 건조한 상태에서 회색을 띄며 참비중은 2.86이고 94 vol.%이상이 미사질이나 모래질 입도로 이루어져 있다. 현무암 석분은 pH 8.3인 약알카리성으로서 중금속 용출시험 결과 환경보전법에서 설정한 Cu, Pb, Zn, Cd, Cr, As, Hg 등 6가지의 중금속 원소가 모두 기준값 이하의 낮은 용출 함량을 보여 농지개량을 위한 객토용으로서의 충분한 이용 가능성을 나타내었다. 현무암 석분은 광물학적 및 물리적·화학적 특성과 폐기물인 석분을 다량 소모시키면서 2차적인 석폐기물을 발생시키지 않아야 한다는 점등을 고려할 때, 결합재의 배합비를 석분에 대한 무게비로 대략 45∼50wt% 정도로 하여 폴리머 복합소재를 이용한 캐스트 제품을 만들어 상품화하는 것이 바람직할 것으로 판단되며, 이를 위해서는 지역 특성에 맞는 다양한 캐릭터 및 몰드 제작은 물론 캐스트용 제품의 고기능화에 대한 지속적인 연구가 요구된다. The sludges mostly of silt or sand size grains (above 94 vol.%) from the Cheolwon district are mainly composed of pyroxene, plagioclase, calcite and K-feldspar, and showed gray colors in dried state. Their true specific gravities and pH's are 2.86 and 8.3, respectively. The sludges from the Cheolwom district have less leached contents of six heavy metals (Cu, Pb, Zn, Cd, Cr, As and Hg) than those of standard levels established in the Soil Environment Conservation Regulation, which impliess that the sludges can be utilized as modifier of soil nature. In consideration of mineralogical, physical and chemical properties and effects of consuming the basalt sludges without secondary stone wastes, some character products had been developed by utilizing the basalt powder sludges with adding binder (unsaturated polyester resin) in 45∼50wt%. Further studies on development of regional character, commercialization of cast products, manufacture of mold and etc. are recommended for more effective utilization of basalt powder sludges.

뇌졸중후 우울증

문석우,서정석,남범우,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

In recent years, depression is most commonly reported psychiatric condition after stroke, poststroke depression(PSD) has attracted world-wide interest. This review focuses on the major research themes that have emerged. Pooled data from studies, have wide variation in results is due to different criteria for patient selection, different evaluation methods, diagnostic criteria and poststroke intervals, conducted throughout the world have found prevalence rates for from 18% to 65%. The diagnosis of PSD is most appropriately based on a structured mental state exam and DSM-N criteria for depression due to stroke with major depressive-like episode or depressive features. Rarely, poststroke patients may also develop bipolar mood disorder. The treatment of PSD, single antidepressant showing efficacy and electroconvulsive theraphy(ECT) and behavioral therapies have also effective. The progression of recovery following stroke can be altered by treating depression, which has been shown to improve recovery in activities of daily living(ADL) and cognitive impairment and to decreased mortality. The mechanisms underlying the association of stroke and mood disorder are important areas for future investigation.