Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Jianyun Wang,Hui Fang,Bingzheng Dong,Dongdong Wang,Yan Li,Xiao Chen,Lijuan Chen,Tong Wei,Qunli Wei 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-β1, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-β1, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Wang, Jianyun,Fang, Hui,Dong, Bingzheng,Wang, Dongdong,Li, Yan,Chen, Xiao,Chen, Lijuan,Wei, Tong,Wei, Qunli The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-${\beta}1$, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-${\beta}1$, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Experimental study on high gravity dam strengthened with reinforcement for seismic resistance on shaking table

Wang, Mingming,Chen, Jianyun,Fan, Shuli,Lv, Shaolan Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.4

In order to study the dynamic failure mechanism and aseismic measure for high concrete gravity dam under earthquake, the comparative models experiment on the shaking table was conducted to investigate the dynamic damage response of concrete gravity dam with and without the presence of reinforcement and evaluate the effectiveness of the strengthening measure. A new model concrete was proposed and applied for maintaining similitude with the prototype. A kind of extra fine wires as a substitute for rebar was embedded in four-points bending specimens of the model concrete to make of reinforced model concrete. The simulation of reinforcement concrete of the weak zones of high dam by the reinforced model concrete meets the similitude requirements. A tank filled with water is mounted at the upstream of the dam models to simulate the reservoir. The Peak Ground Acceleration (PGA) that induces the first tensile crack at the head of dam is applied as the basic index for estimating the overload capacity of high concrete dams. For the two model dams with and without strengthening tested, vulnerable parts of them are the necks near the crests. The results also indicate that the reinforcement is beneficial for improving the seismic-resistant capacity of the gravity dam.

Experimental study on high gravity dam strengthened with reinforcement for seismic resistance on shaking table

Mingming Wang,Jianyun Chen,Shuli Fan,Shaolan Lv 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.4

In order to study the dynamic failure mechanism and aseismic measure for high concrete gravity dam under earthquake, the comparative models experiment on the shaking table was conducted to investigate the dynamic damage response of concrete gravity dam with and without the presence of reinforcement and evaluate the effectiveness of the strengthening measure. A new model concrete was proposed and applied for maintaining similitude with the prototype. A kind of extra fine wires as a substitute for rebar was embedded in four-points bending specimens of the model concrete to make of reinforced model concrete. The simulation of reinforcement concrete of the weak zones of high dam by the reinforced model concrete meets the similitude requirements. A tank filled with water is mounted at the upstream of the dam models to simulate the reservoir. The Peak Ground Acceleration (PGA) that induces the first tensile crack at the head of dam is applied as the basic index for estimating the overload capacity of high concrete dams. For the two model dams with and without strengthening tested, vulnerable parts of them are the necks near the crests. The results also indicate that the reinforcement is beneficial for improving the seismicresistant capacity of the gravity dam.

Experimental and Numerical Comparative Study on Gravity Dam-Reservoir Coupling System

Mingming Wang,Jianyun Chen,Weirong Xiao 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.10

A 203m high gravity dam-reservoir coupling system in earthquake is studied experimentally and numerically in this work. The dynamic model test is performed on a shaking table, and the dynamic process of the coupling system is simulated with two numerical methods. The natural frequency, hydrodynamic pressure on upstream and acceleration amplification factors along the dam height are obtained from the test and the methods. It is found that the results from FSCM agree better with those from test compared with AMM. So the FSCM should be the first choice to analysis the dam-reservoir coupling system interaction under earthquake. The AMM, which is frequently used in the Code for Seismic Design of Hydraulic Structures of many countries, needs to be modified by a factor smaller than 1. The factor varies along the height of the dam according to its shape, reservoir depth and higher modes and so on. Finally, the reduction factors of the AMM along dam height are suggested in this work.

Performance validation and application of a mixed force-displacement loading strategy for bi-directional hybrid simulation

Zhen Wang,Qiyang Tan,Pengfei Shi,Ge Yang,Siyu Zhu,Guoshan Xu,Bin Wu,Jianyun Sun 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.26 No.3

Hybrid simulation (HS) is a versatile tool for structural performance evaluation under dynamic loads. Although real structural responses are often multiple-directional owing to an eccentric mass/stiffness of the structure and/or excitations not along structural major axes, few HS in this field takes into account structural responses in multiple directions. Multi-directional loading is more challenging than uni-directional loading as there is a nonlinear transformation between actuator and specimen coordinate systems, increasing the difficulty of suppressing loading error. Moreover, redundant actuators may exist in multi-directional hybrid simulations of large-scale structures, which requires the loading strategy to contain ineffective loading of multiple actuators. To address these issues, lately a new strategy was conceived for accurate reproduction of desired displacements in bi-directional hybrid simulations (BHS), which is characterized in two features, i.e., iterative displacement command updating based on the Jacobian matrix considering nonlinear geometric relationships, and force-based control for compensating ineffective forces of redundant actuators. This paper performs performance validation and application of this new mixed loading strategy. In particular, virtual BHS considering linear and nonlinear specimen models, and the diversity of actuator properties were carried out. A validation test was implemented with a steel frame specimen. A real application of this strategy to BHS on a full-scale 2-story frame specimen was performed. Studies showed that this strategy exhibited excellent tracking performance for the measured displacements of the control point and remarkable compensation for ineffective forces of the redundant actuator. This strategy was demonstrated to be capable of accurately and effectively reproducing the desired displacements in large-scale BHS.

Green preparation of lipase@Ca3(PO4)2 hybrid nanoflowers using bone waste from food production for efficient synthesis of clindamycin palmitate

Anming Wang,Xinxin Chen,Jianyun Yu,Ningning Li,Huimin Li,Youcheng Yin,Tian Xie,Stephen Gang Wu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.89 No.-

To prepare enzyme@Ca3(PO4)2 for environmentally friendly biocatalysis, Ca2+ and (PO4)3 were extractedfrom bone waste by acidification. In the nearly neutralizedfiltrate, the Ca2+ and (PO4)3 formed aCa3(PO4)2 sediment that crystallized on a template of Thermomyces lanuginosus lipase (TLL) at 4 ℃ for24 h, producing enzyme@Ca3(PO4)2-Bone hybrid nanoflowers (hNFs). Clindamycin palmitate wasefficiently synthesized by transfer of a palmityl moiety from vinyl palmitate to clindamycin free baseusing these hNFs as a biocatalyst. At 30 ℃ in petroleum ether (PE) as the solvent, the yield of the TLL hNFcatalyzedreaction was as high as 70.0%. Even at a high temperature (80 ℃), the yield in the hNF-catalyzedreaction was still 52.6%, but no product was detected when using free lipase as the catalyst. Moreover, thehNFs retained 90% of their initial activity after 10 cycles (120 h, 12 h per cycle). This green and sustainablemethod that utilizes bone waste from food production as the raw source of the inorganic component wasfacile and efficient, and the system may also be applicable for preparing other enzyme@Ca3(PO4)2 hNFsfor industrial applications.

Analysis of transcriptomic profiles and physiological traits of exogenous 24-epibrassinolide alleviating salt stress in Atractylodes macrocephala Koidz.

Zhang Jianyun,Fang Huiyong,Jiang Jianming,Gu Xian,Zhao Huimei,Qiu Jinmiao,Wang Qian,Zheng Yuguang,Zheng Kaiyan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Exogenous 24-epibrassinolide (24-epiBL) application is a preferred method for improving salt tolerance in plants. However, information about the effects of exogenous 24-epiBL application related to salt response in Atractylodes macrocephala Koidz. (AMK) has rarely been reported. Here, we aimed to investigate the effects of exogenous 24-epiBL (0.01 mg L−1) on enhancing salt tolerance in AMK seedlings under salt stress (5 g L−1 NaCl) based on seed germination experiments. Our results indicated that NaCl treatment suppresses AMK seed germination, while 24-epiBL treatment under nonstress and stress conditions had positive effects on plant seedling growth. Treatment with NaCl significantly inhibited radicle elongation and root activity to endure salt stress, and 24-epiBL treatment promoted growth and alleviated the damage to osmotic stress, ion stress sub- stances, and oxidative stress by regulating free proline (Pro) and soluble protein (SP) contents and enhancing soluble sugar (SS) conversion. Moreover, the expression of normal development process genes was affected, and differentially expressed genes (DEGs) in multiple pathways associated with amino acid metabolism, signal transduction, carbohydrate metabolism, and lipid metabolism were regulated under NaCl treatment. Further analysis of the AMK transcriptome profile revealed that exogenous 24-epiBL enhanced salt tolerance through hormone regulation and carbohydrate metabolism promotion, salt- responsive gene expression inhibition, and induction of pathways related to terpenoid and polyketide metabolism as well as pentose and glucuronate interconversions for plant growth promotion. Collectively, our findings successfully demonstrate that exogenous 24-epiBL plays a positive role in regulating the AMK response to salt stress, which provides potential genetic resources to improve salt tolerance in plants.