Alcohol Dehydrogenase Isozyme in Rice(Oryza sativa complex)

CHEN Lijuan,SUH Hak Soo,LEE Dongsun 한국작물학회 2000 한국작물학회 학술발표대회 논문집 Vol.2000 No.1

A Ultra-Low Specific On-Resistance and Extended Gate SJ LDMOS Structure

Lijuan Wu,Jiaqi Chen,Hang Yang,QiLin Ding,Xing Chen,Shaolian Su 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.3

A new structure of extended gate (EG) SJ LDMOS is proposed in this paper to overcome the substrate assisted depletion (SAD) eff ect in the structure of Super-Junction lateral double diff used metal oxide semiconductor (SJ LDMOS). Different from other surface SJ structures, the SJ layer of the structure is located in the body of the drift region. Gate oxide and silicon layer form the EG Structure-Oxide-Semiconductor structure that is similar to Metal–Insulator–Semiconductor capacitor. The the N-drift of the EG structure can obtain the charge compensation to overcome the SAD eff ect, and a nearly rectangular electric field is achieved. In the on-state, the EG structure has two conductive channels and the accumulation layer is formed on the drift region. By accumulating high concentration electrons in the channels, the specifi con-resistance ( R ON,sp ) is greatly reduced. When the drift length is 24 μm, 431.4 V breakdown voltage ( V B ) and 9.8 mΩ cm 2 R ON,sp are achieved, and the fi gure of merit is 18.9 MW cm −2 .

Long-term continuously monocropped peanut significantly disturbed the balance of soil fungal communities

Chen Mingna,Zhang Jiancheng,Liu Hu,Wang Mian,Pan LiJuan,Chen Na,Wang Tong,Jing Yu,Chi Xiaoyuan,Du Binghai 한국미생물학회 2020 The journal of microbiology Vol.58 No.7

Balancing soil microbial diversity and abundance is critical to sustaining soil health, and understanding the dynamics of soil microbes in a monocropping system can help determine how continuous monocropping practices induce soil sickness mediated by microorganisms. This study used previously constructed gradient continuous monocropping plots and four varieties with different monocropping responses were investigated. The feedback responses of their soil fungal communities to short-term and long-term continuous monocropping were tracked using high-throughput sequencing techniques. The analyses indicated that soil samples from 1 and 2 year monocropped plots were grouped into one class, and samples from the 11 and 12 year plots were grouped into another, regardless of variety. At the species level, the F. solani, Fusarium oxysporum, Neocosmospora striata, Acrophialophora levis, Aspergillus niger, Aspergillus corrugatus, Thielavia hyrcaniae, Emericellopsis minima, and Scedosporium aurantiacum taxa showed significantly increased abundances in the long-term monocropping libraries compared to the short-term cropping libraries. In contrast, Talaromyces flavus, Talaromyces purpureogenus, Mortierella alpina, Paranamyces uniporus, and Volutella citrinella decreased in the long-term monocropping libraries compared to the shortterm libraries. This study, combined with our previous study, showed that fungal community structure was significantly affected by the length of the monocropping period, but peanut variety and growth stages were less important. The increase in pathogen abundances and the decrease in beneficial fungi abundances seem to be the main cause for the yield decline and poor growth of long-term monocultured peanut. Simplification of fungal community diversity could also contribute to peanut soil sickness under long-term monocropping. Additionally, the different responses of peanut varieties to monocropping may be related to variations in their microbial community structure.

Study on the grading standard of Panax notoginseng seedlings

Chen, Lijuan,Yang, Ye,Ge, Jin,Cui, Xiuming,Xiong, Yin The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.2

Background: The quality differences in seedlings of medicinal herbs often affect the quality of medicinal parts. The establishment of the grading standard of Panax notoginseng seedlings is significant for the stable quality of medicinal parts of P. notoginseng. Methods: To establish the grading standard of P. notoginseng seedlings, a total of 36,000 P. notoginseng seedlings were collected from 30 producing areas, of which the fresh weight, root length, root diameter, bud length, bud diameter, and rootlet number were measured. The K-means clustering method was applied to grade seedlings and establish the grading standard. Results: The fresh weight and rootlet number of P. notoginseng seedlings were determined as the final indices of grading. P. notoginseng seedlings from different regions of Yunnan could be preliminarily classified into four grades: the special grade, the premium grade, the standard grade, and culled seedlings. Conclusion: The grading standard was proven to be reasonable according to the agronomic characters, emergence rate, and photosynthetic efficiency of seedlings after transplantation, and the yields and contents of active constituents of the medicinal parts from different grades of seedlings.

PCR-RFLP Analysis of mtDNA in the Genus Oryza

CHEN Lijuan,SUH Hak Soo,LEE Dongsun 한국작물학회 2000 한국작물학회 학술발표대회 논문집 Vol.2000 No.1

Identification of Ldh Isozyme Locus in Rice(Oryza sativa complex)

CHEN Lijuan,SUH Hak Soo,LEE Dongsun 한국작물학회 2000 한국작물학회 학술발표대회 논문집 Vol.2000 No.1

PCR-RFLP Analysis of cpDNA in the Genus Oryza

CHEN Lijuan,SUH Hak Soo,LEE Dongsun 한국작물학회 2000 한국작물학회 학술발표대회 논문집 Vol.2000 No.1

Evaluation of the effect of aggregate on concrete permeability using grey correlation analysis and ANN

Lijuan Kong,Xiaoyu Chen,Yuanbo Du 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.17 No.5

In this study, the influence of coarse aggregate size and type on chloride penetration of concrete was investigated, and the grey correlation analysis was applied to find the key influencing factor. Furthermore, the proposed 6-10-1 artificial neural network (ANN) model was constructed, and performed under the MATLAB program. Training, testing and validation of the model stages were performed using 81 experiment data sets. The results show that the aggregate type has less effect on the concrete permeability, compared with the size effect. For concrete with a lower w/b, the coarse aggregate with a larger particle size should be chose, however, for concrete with a higher w/c, the aggregate with a grading of 5-20 mm is preferred, too large or too small aggregates are adverse to concrete chloride diffusivity. A new idea for the optimum selection of aggregate to prepare concrete with a low penetration is provided. Moreover, the ANN model predicted values are compared with actual test results, and the average relative error of prediction is found to be 5.62%. ANN procedure provides guidelines to select appropriate coarse aggregate for required chloride penetration of concrete and will reduce number of trial and error, save cost and time.

Bond Behaviors between Ribbed BFRP Bars and Ecological High Ductility Cementitious Composites

Lijuan Chai,Liping Guo,Bo Chen,Jiandong Wu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.11

Direct pull-out method was conducted to explore the bond stress-slip response of ribbed basalt fiber reinforced polymer (BFRP) bars embedded in ecological high ductility cementitious composites (Eco-HDCCs). The effects of the embedment length and cover thickness on the bond properties between Eco-HDCCs and the bars were described. Results indicate that the failure characteristics of the specimens depend on the embedment length and cover thickness of the BFRP bars. As the embedment lengths of the bars increase, the ultimate bond stress decreases while the ultimate slip increases. Besides, both the ultimate bond stress and ultimate slip show an increasing trend if the specimen has a cover thickness of less than 45 mm, while the increasing trend is slower if the specimen has a cover thickness of more than 45 mm. In addition, the strain of the specimen surface depends on the cover thickness, and the strain increases when the cover thickness is in a range of 15 mm − 35 mm. Moreover, certain embedment lengths and cover thicknesses of the BFRP bars are recommended in the structural design of bridge deck link slabs. The bond stress-slip relationship models of the BFRP bars in Eco-HDCCs are proposed based on the test results.

CRISPR/Cas9‑mediated editing of CsWRKY22 reduces susceptibility to Xanthomonas citri subsp. citri in Wanjincheng orange (Citrus sinensis (L.) Osbeck)

Lijuan Wang,Shanchun Chen,Aihong Peng,Zhu Xie,Yongrui He,Xiuping Zou 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.5

Key message CRISPR/Cas9-mediated editing of CsWRKY22 repressed canker development in Wanjincheng orange. Abstract Citrus canker, a destructive disease of citrus, is threatening the citrus industry worldwide. Breeding resistant cultivars is the most economical and effective approach to control citrus canker. Recently, CRISPR/Cas9-mediated genome editing was demonstrated to be a powerful tool for the improvement of citrus resistance to the disease. In our previous works, we confirmed that CsWRKY22 is involved in the plant immunity response to citrus canker in Wanjincheng orange (Citrus sinensis (L.) Osbeck). In this study, we targeted this gene to improve the resistance of Wanjincheng orange against citrus canker by CRISPR/Cas9-mediated editing. Sanger sequencing confirmed that CsWRKY22 in Wanjincheng orange contains CsWRKY22G and CsWRKY22C alleles, and the ratio of CsWRKY22G to CsWRKY22C is approximately 2:1. Four sgRNAs, which targeted the first exon of CsWRKY22, were selected for testing. In vitro cleavage activity analysis showed that two (W1 and W2) of the four sgRNAs displayed robust cleavage activities using PCR amplicons from the Wanjincheng orange genome as template. Subsequently, two constructs, pCas9/WRKY22sgRNA-W1 and pCas9/WRKY22sgRNA-W2, were used to modify the CsWRKY22. Three mutant plants were identified from seven independent transgenic plants. Based on Sanger sequencing, the W1-1, W2-2, and W2-3 mutant lines displayed 85.7%, 79.2%, and 68.2% mutation rates, respectively. Resistance evaluation indicated that the mutant plants showed decreased susceptibility to citrus canker. These results indicate that CRISPR/Cas9-targeted gene modification is an efficient approach for enhancing disease resistance in citrus.