Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema Lectin and chemical modification properties on its mannose-binding sites

( Bo Liu ),( Xiao Chao Xu ),( Yan Cheng ),( Jian Huang ),( Yan Hong Liu ),( Zhen Liu ),( Ming Wei Min ),( He Jiao Bian ),( Jing Chen ),( Jin Ku Bao ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.5

Clinicopathologic Characteristics and Prognostic Factors in Patients with Operable HER-2 Overexpressing Breast Cancer

Liu, Ai-Na,Sun, Ping,Liu, Jian-Nan,Ma, Jin-Bo,Qu, Hua-Jun,Zhu, Hua,Yu, Cai-Yan,Zhang, Liang-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To study the relationship between clinical pathologic characteristics, treatment modalities and prognostic factors in HER-2 (Human Epidermal growth factor Receptor-2) overexpressed breast carcinoma. Materials and Methods: Major clinico-pathological factors including therapeutic modalities and survival status of 371 breast cancer patients with HER2 over-expression, teated at Yantai Yuhuangding Hospital from March of 2002 to December of 2010 were retrospectively studied, with special attention focused on survival-related factors. Results: The median age of the total 371 patients in this study was 48 years at time of diagnosis, among which, the leading pathological type was infiltrating ductal carcinoma (92.5%); 62.8% presented with a primary tomor larger than 2 cm in diameter at diagnosis, 51.0% had axillary lymph node (ALN) metastases; ER (Estrogen receptor)/PR (Progesterone receptor) double negative occured in 52.8% of cases, and PCNA (proliferation cell nuclear antigen) (+++) was found in 55.1%. HER-2 overexpressed patients were usually in advanced stage when the diagnosis was made (72.8% at stages IIA~IIIC). The prognosis and survival were assessed in 259 patients with complete follow-up data. 5-year DFS (disease-free survival) and OS (overall survival) rate was 68.0% and 78.0% respectively. Univariate analysis revealed that age, tumor size, ALN metastases, LVSI (lymph-vascular space involvement), PCNA status, hormonal therapy, chemotherapy cycles, and HER-2 overexpression, correlated closely with the prognosis. ALN metastases, LVSI, PCNA status and chemotherapy cycles were independent predictors of survival. Conclusions: HER-2 overexpressed breast cancer has special clinical and pathological characteristics, with advanced clinical stages and high rate of ER/PR double negative. Lymph node metastases, LVSI, PCNA and chemotherapy cycles are independent predictors of prognosis.

Cervical Cancer Gene Therapy by Gene Loaded PEG-PLA Nanomedicine

Liu, Bo,Han, Shu-Mei,Tang, Xiao-Yong,Han, Li,Li, Chang-Zhong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background and Aims: Advances in the treatment of cervical cancer over the last decade have predominantly involved the development of genes directed at molecular targets. Gene therapy is recognized to be a novel method for the treatment of cervical cancer. Genes can be administered into target cells via nanocarriers. This study aimed to develop systemically administrable nano-vectors. Floate (Fa) containing gene loaded nanoparticles (NPs) could target HeLa human cervical cancer cells through combination with receptors on the cells to increase the nuclear uptake of genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly (D, L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro transfection efficiency of Fa-NPs/DNA was evaluated in HeLa cells and human umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183 nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher than naked DNA in HeLa cells. However, in HUVECs, no significant difference appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function as excellent materials for gene loading. This nano-approach could be used as tumor cell targeted medicine for the treatment of cervical cancer.

A method for underwater image analysis using bi-dimensional empirical mode decomposition technique

Liu, Bo,Lin, Yan Techno-Press 2012 Ocean systems engineering Vol.2 No.2

Recent developments in underwater image recognition methods have received large attention by the ocean engineering researchers. In this paper, an improved bi-dimensional empirical mode decomposition (BEMD) approach is employed to decompose the given underwater image into intrinsic mode functions (IMFs) and residual. We developed a joint algorithm based on BEMD and Canny operator to extract multi-pixel edge features at multiple scales in IMFs sub-images. So the multiple pixel edge extraction is an advantage of our approach; the other contribution of this method is the realization of the bi-dimensional sifting process, which is realized utilizing regional-based operators to detect local extreme points and constructing radial basis function for curve surface interpolation. The performance of the multi-pixel edge extraction algorithm for processing underwater image is demonstrated in the contrast experiment with both the proposed method and the phase congruency edge detection.

Comprehensive Study on Associations Between Nine SNPs and Glioma Risk

Liu, Hai-Bo,Peng, Yu-Ping,Dou, Chang-Wu,Su, Xiu-Lan,Gao, Nai-Kang,Tian, Fu-Ming,Bai, Jie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Aim: Glioma cancer is the most common type of adult brain tumor. Recent genome-wide association studies (GWAS) have identified various new susceptibility regions and here we conducted an extensive analysis of associations between 12 single nucleotide polymorphisms (SNPs) and glioma risk. Methods: A total of 197 glioma cases and 197 health controls were selected, and 9 SNPs in 8 genes were analyzed using the Sequenom MassARRAY platform and Sequenom Assay Design 3.1 software. Results: We found the MAF among selected controls were consistent with the MAF from the NCBI SNP database. Among 9 SNPs in 8 genes, we identified four significant SNP genotypes associated with the risk of glioma, C/C genotype at rs730437 and T/T genotype at rs1468727 in ERGF were protective against glioma, whereas the T/T genotype at rs1799782 in XRCC1 and C/C genotype at rs861539 in XRCC3 conferred elevated risk. Conclusion: Our comprehensive analysis of nine SNPs in eight genes suggests that the rs730437 and rs1468727 in ERGF, rs1799782 in XRCC1 gene, and rs861539 in XRCC3 gene are associated with glioma risk. These findings indicate that genetic variants of various genes play a complex role in the development of glioma.

Nano-Actuator Based High Performance Head-Disk Systems

Bo Liu,Chee-How Wong,Wei Hua,Sheng-Kai Yu 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Molecular engineering of indoline based organic sensitizers for highly efficient dye-sensitized solar cells

Liu, Bo,Liu, Qingbin,You, Dan,Li, Xiaoyan,Naruta, Yoshinori,Zhu, Weihong The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.26

<P>The increasing electron-donating ability of the donor part is focused to further optimize the light-harvesting capability. Our strategy is to introduce an additional donor group into the indoline unit in the donor part to form a donor–donor structure (D–D moiety). Three different units (carbazole, fluorene and 4-methylphenyl groups) with different degrees of electron-donating capability are incorporated, thus constructing the specific donor–donor–π–acceptor (D–D–π–A) system (C-CA, F-CA and I-3) and giving a systematic view of the absorption evolution. Through molecular engineering, their light-harvesting capabilities, energy levels and photovoltaic performances were studied. As expected, utilizing strong electron-donating carbazole unit as additional donor, the IPCE spectrum of DSSC based on C-CA is successfully broadened to NIR region on the premise of suitable LUMO level, with an extraordinarily high plateau in visible region till around 700 nm. In the system of C-CA and F-CA, the introduction of <I>n</I>-pentyl group in donor part of carbazole and fluorene unit has little effect on preventing the molecular π-aggregation due to the good co-planarity of π-linker (vinyl thiophene), suggesting that the most effective way to prevent π-aggregation is still the incorporation of long alkyl groups into planar π-linker segment. However, the introducing long alkyl group can effectively prevent the electron recombination between electrons in conduction band (CB) of TiO<SUB>2</SUB> and I<SUB>3</SUB><SUP>−</SUP> ions. Along with the preferable light-harvesting capability, C-CA presents excellent IPCE performance with a short-circuit photocurrent (<I>J</I><SUB>sc</SUB>) of 18.53 mA cm<SUP>−2</SUP>, an open-circuit photovoltage (<I>V</I><SUB>oc</SUB>) of 649 mV, a fill factor of 0.71, corresponding to a power conversion efficiency (<I>η</I>) of 8.49%. The internal relations between chemical structure and conversion efficiency provide a strategy for developing highly efficient organic sensitizers working in whole visible region with high photovoltaic performance.</P> <P>Graphic Abstract</P><P>Utilizing a carbazole unit as additional donor, the photoresponse of C-CA is broadened to the NIR region with a <I>η</I> of 8.49%. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm31704d'> </P>

Behavior of reinforced concrete beams filled with demolished concrete lumps

Bo Wu,Zhe Xu,Zhongguo John Ma,Qiongxiang Liu,Wei Liu 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.3

In the past decades, recycling use of demolished concrete was almost limited to the types of recycled coarse aggregate with a size of about 5-40 mm and recycled fine aggregate with a size of about 0-5 mm for concrete structures, and reuse of demolished concrete lumps (DCLs) with a size much larger than that of recycled aggregate, e.g., 50-300 mm, has been limited to roadbed, backfilling materials, or discarded to landfills. Treatment processes of DCLs are much simpler than those of recycled aggregate, leading to less cost and more energy-saving. In the future, the amount of demolished concrete is estimated to be much higher, so reuse of DCLs for concrete structures will become necessary. The objectives of this paper are to document the process of making reinforced concrete beams with DCLs, and to discuss the flexural and shear behaviors of those reinforced DCL beams through an experimental program, which includes three beams filled with DCLs and one conventional beam for investigating the flexural strengths and deformations, and 12 beams filled with DCLs and two conventional beams for investigating the shear strengths and deformations. The authors hope that the proposed concept offers another sustainable solution to the concrete industry.

Detection of the Middle and the Upper Atmosphere over Wuhan with a Fe Fluorescence/Boltzmann Temperature Lidar

Bo Liu,Fan Yi,Jun zhou 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.1

In recent years, the detection and investigation of the middle and the upper atmosphere have been quite active subjects. To acquire information on the middle and the upper atmosphere, we developed the first Fe fluorescence/Boltzmann temperature lidar in Asia. In this paper, the system configuration and data-processing methods of the lidar are described, and some results of lidar observations are presented.

Development and comparison of whole-cell assay systems for quorum-sensing inhibitors based on TraR, LasR, and QscR.

Liu, Hai-Bo,Kim, Jung Sun,Park, Sunghoon Mary Ann Liebert, Inc 2011 Journal of biomolecular screening Vol.16 No.9

<P>Quorum sensing (QS) is a cell density-dependent signaling system that is used by bacteria to coordinate gene expression within their population. In this study, the authors describe the development and characterization of various cell-based bioassay systems for detecting QS inhibitors based on three LuxR family proteins, TraR, LasR, and the recently identified QscR. Three different gram-negative bacteria, Escherichia coli, Agrobacterium tumefaciens, and Pseudomonas aeruginosa, were employed as reporter strains to overproduce one of the aforementioned QS activator proteins and respond to inhibitors. The nine different whole-cell assay systems (three reporter strains three QS proteins) were evaluated for their applicability and reliability by studying quantitative responses to various furanones, which are potent inhibitors of the LuxR family proteins. These results demonstrate that the cell-based bioassay systems are sensitive and reliable tools for screening of QS activators and inhibitors. This study also suggests that furanones are potentially important QS inhibitors for many LuxR-type activator proteins.</P>