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Treatment of Mycoplasma wenyonii Infection in Cows with Imidocarb Dipropionate Injection-acupuncture
Jianzhu Liu,Zhengui Yan,Tiantian Chen,Ziqiang Cheng,Huijun Guo,Zhenyong Wang,Yong Wang 사단법인약침학회 2008 Journal of Acupuncture & Meridian Studies Vol.1 No.2
Sixteen shorthorn cows from Xiazhuang farm were admitted to the Veterinary Teaching Hospital at the College of Animal Science and Veterinary Medicine, Shandong Agricultural University for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. Blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with Mycoplasma wenyonii. The subjects were divided into two groups: control group (three cows) treated with intramuscular injection with imidocarb dipropionate (3 mg/kg/day for 2 days) and the experimental group (13 cows), treated with injection-acupuncture (Imidocarb Dipropionate, 1 mg/kg, once every 3 days for 6 days) at BL17, BL18, BL20, BL25, ST36, SP06 and CV04. At day 15, negative results were found using blood smear examination in all control and experimental groups. Sixteen shorthorn cows from Xiazhuang farm were admitted to the Veterinary Teaching Hospital at the College of Animal Science and Veterinary Medicine, Shandong Agricultural University for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. Blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with Mycoplasma wenyonii. The subjects were divided into two groups: control group (three cows) treated with intramuscular injection with imidocarb dipropionate (3 mg/kg/day for 2 days) and the experimental group (13 cows), treated with injection-acupuncture (Imidocarb Dipropionate, 1 mg/kg, once every 3 days for 6 days) at BL17, BL18, BL20, BL25, ST36, SP06 and CV04. At day 15, negative results were found using blood smear examination in all control and experimental groups.
The therapeutic effect ozone on contaginous ecthyma occurred in Korean Native Goats
Jianzhu Liu,Min-Sung Hong,Ji-Min Seo,Sung-Nam Jo,Jung-Yeon Lee,Kun-Ho Song,Duck-Hwan Kim 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.1
본 연구에서는 충남 공주근교에서 사육중인 한국재래산양에서 자연 발생한 전염성 농포성 피부염에 대하여 오존의 치료효과를 조사하였다. 전염성 농포성 피부염은 한국재래산양 150두 중 130두에서 발생하였다. 16두(2개월-2 년)를 선발하여 대조군(5두), ozonated oil군(5두) 및 ozonated water군(6두)으로 구분하였다. 대조군에서는 실험기간 중 아무런 치료를 하지 않았으며, ozonated oil군은 식물성유에 오존가스(200ppm)로 3일간 bubbling한 ozonated oil을 환부에 1일 1회 2주간 도포하였고, ozonated water군은 수도물에 오존가스(200ppm)로 1시간 동안 bubbling한 ozonated water(0.1ppm)를 2주간 자유로이 음수토록 하였다. 그 결과, 치료율은 ozonated oil군이 80%(4/5두), ozonated water군이 66.7%(4/6두)로, 대조군(20%, 1/5두) 보다 양호하였다. 이 결과를 근거로 전염성 농포성 피부염에 이환된 한국재래 산양 121두를 대상으로 ozonated oil을 1일 1회, 30일간 환부에 도포하였다. 그 결과, 환축의 모든 병변부는 완치되었다. 이상의 결과를 종합해 볼 때, 오존요법은 산양의 전염선 농포성 피부염의 치료에 유효한 것으로 판단되었다.
Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus
Na Geng,Kangping Liu,Jianwei Lu,Yuliang Xu,Xiaozhou Wang,Run Wang,Jianzhu Liu,Yongxia Liu,Bo Han 한국미생물학회 2020 The journal of microbiology Vol.58 No.4
Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The results showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.
Acupuncture Treatment in a Case with Equine Conjunctivitis
김덕환,Jianzhu Liu,최석화,Phillip MacManus,Paoraic Jennings,Karl Darcy,Fiona Burke,Nola Leorald,Phil A. M. Rogers 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3
One male Arabian horse, 5-year-old, reared in Gort, Co Galway, Ireland was diagnosed as conjunctivitis. Acupuncture and aquapuncture with dexamethasone were done at BL01, BL02 and ST01, twice a week, and oculoacupuncture was done into Shangjiao area using with oculo-acupuncture needle for human use. At session 3, there were no lacrimation, ocular discharge and hyperemia in the eye. The present patient was a case with equine conjunctivitis which showed favorable therapeutic response by acupuncture treatment
Acupuncture treatment of torticollis in a foal
Kim, Duck-Hwan,Liu, Jianzhu,Lee, Jung-Yeon,MacManus, Philip,Jennings, Padraic,Darcy, Karl,Burke, Fiona,Rogers, Philip A.M. The Korean Society of Veterinary Science 2006 大韓獸醫學會誌 Vol.46 No.1
A 6-month-old thoroughbred filly foal had torticollis and circled towards the right side. A local veterinarian treated her twice using dexamethasone for 1 week but there was little clinical improvement. Needles were inserted into Ting points of both hind limb and one ocular acupoint (shang jiao area from GB01). Injection acupuncture (dexamethasone, twice/week) was used at GV16, GB20, BL10 and LU07, for the neck and head. Acupoint GB34 was added to those points at session 4. In addition, a trigger point in the left neck was injected with 0.2 ml ($200{\mu}g$ of apitoxin) of bee-venom diluted with 1 ml of 2% lidocaine from session 1 to session 3. At session 2, the symptoms had ameliorated a little. At session 3, they were much improved; the right ear was completely normal and the neck could be moved about 60% of normal range. At session 4, nodding was possible and stiffness of the neck was much improved, having returned to about 80% of normal range. Three days after session 4, the symptom of torticollis had disappeared completely and the foal could walk in a straight line. In conclusion, the present patient was a case with equine torticollis which showed favourable therapeutic response by combination of needle-acupuncture plus injection-acupuncture with dexamethasone and apitoxin.
Cho, Sung-nam,Liu, Jianzhu,Jun, Hyung-kyou,Cho, Sung-whan,Chang, Kyu-seob,Kim, Seong-ju,Kim, Young-hee,Yang, Hyo-jin,Kim, Duck-hwan The Korean Society of Veterinary Science 2007 大韓獸醫學會誌 Vol.47 No.3
The present study was performed to clarify the recovery effect of polyacetylene constituents from Acanthopanax senticosus ethanol extracts on $CCl_4$-induced acute hepatic injury in dogs. Sixteen clinically healthy dogs were divided into the control group (six dogs), and experimental group I (three dogs), II (three dogs) and III (four dogs). Acute hepatic injury was induced by single intraperitoneal injection of 1 ml/kg $CCl_4$ solution ($CCl_4$ : olive oil = 1 : 1) after sterilization. The control group was orally administered with 10 ml of corn oil, and experimental groups I, II and III were orally administered with extracts of A. senticosus at the dose of 5, 10 and 30 mg/kg in 10 ml corn oil for seven consecutive days at 24 h after $CCl_4$ injection. We assessed changes occurring in serum ALT, AST and GGT activities on the pre, day 0, day 1, day 2, day 3, day 5 and day 7, together with the histopathological findings. In experimental group I, the significant changes in serum ALT activities were found on day 5 (p<0.05) and day 7 (p<0.05), compared with those in the control group. However, significant differences were not found in the changes of serum AST and GGT activities. In experimental group II, significant changes were found on day 2 (p<0.05), day 3(p<0.05), day 5 (p<0.05) and day 7 (p<0.05) in serum ALT activities, on day 1 (p<0.05) in serum AST, and on day 2 (p<0.05) and day 3 (p<0.05) in serum GGr activities, respectively. In experimental group III, the changes in serum ALT, AST and GGT activities were not significant. In the histopathological evaluation, experimental group I and II showed much improvement, while experimental group III became worse when compared with the control group. Based on the results of the present study, it was concluded that low dose (below 10 mg/kg) administration of polyacetylene constituents from A. senticosus ethanol extracts was effective for recovery of acute hepatic injury induced by $CCl_4$ in dogs.