Enhancement of Pyruvate Production by Torulopsis glabrata through Supplement of Oxaloacetate as Carbon Source

Jian Chen,Li-Ming Liu,Guo-Cheng Du,Yin Li,Hua-Zhong Li 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

The capability of utilizing a TCA cycle intermediates as the sole carbon source by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019 was demonstrated with plate count method. It is indicated that T. glabrata could grew on a medium with one of the TCA cycle intermediates as the sole carbon source, but more colonies were observed when glucose, acetate and one of the TCA cycle intermediates coexisted in the medium. Among the intermediates of the TCA cycle examined in this study, cell growth was improved by supplementing oxaloacetate. Further investigation showed that the presence of acetate was necessary when oxaloacetate was supplemented. By supplementing with 10 g/L of oxaloacetate in pyruvate batch fermentation, dry cell weight increased from 11.8 g/L to 13.6 g/L, and pyruvate productivity was enhanced from 0.96 gL-1h-1 to 1.19 gL-1h-1 after cultivation of 56 h. The yield of pyruvate to glucose was also improved from 0.63 g/g to 0.66 g/g. These results indicate that under vitamins limitation, the productivity and yield of pyruvate could be enhanced via an increase of cell growth by the supplementation of oxaloacetate.

XPD Lys751Gln and Asp312Asn Polymorphisms and Gastric Cancer Susceptibility: A Meta-analysis of Case-control Studies

Yin, Qing-Hua,Liu, Chuan,Hu, Jian-Bing,Meng, Rong-Rong,Li, Lian,Wang, Ya-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Published data regarding the association between xeroderma pigmentosum group D (XPD) Lys751Gln and Asp312Asn polymorphisms and gastric cancer susceptibility havew been inconclusive. This meta-analysis was therefore performed toobtain a more precise estimation of any relationship. Materials and Methods: A comprehensive literature search was conducted to identify all case-control studies of Lys751Gln and Asp312Asn polymorphisms and susceptibility to gastric cancer. Summary odds ratios (ORs) and its 95% confidence intervals (95% CIs) were calculated using a random-effects model with the software STATA (version10.0). Results: A total of 12 case-control studies including 3,147 cases and 4,736 controls were included. Overall, no significant associations were found in some models (for Lys751Gln: Lys/Gln vs Lys/Lys: OR=1.144, 95% CI=0.851-1.541, Gln/Gln vs Lys/Lys: OR=1.215, 95% CI = 0.740-1.955, dominant model: OR=1.137, 95% CI=0.818-1.582; recessive model: OR=1.123, 95% CI=0.765-1.650; for Asp312Asn: Asp/Asn vs Asp/Asp: OR=1.180, 95% CI=0.646-2.154, dominant model: OR=1.380, 95% CI = 0.812-2.346), but significantly elevated susceptibility was found for Asp312Asn polymorphism in some models (Asn/Asn vs Asp/Asp: OR=2.045, 95% CI=1.254-3.335, recessive model: OR=1.805, 95% CI =1.219-2.672), for the additive model, the XPD Lys751Gln and Asp312Asn polymorphisms were not significantly associated with gastric cancer susceptibility. In stratified analyses, significantly elevated susceptibility was found for some models in the Chinese population. Conclusion: This meta-analysis suggested the XPD Asp312Asn polymorphism might be a potential biomarker of gastric cancer susceptibility in overall population, while both XPD Lys751Gln and Asp312Asn polymorphisms might be risk factors of gastric cancer susceptibility in Chinese.

S100A4 Gene is Crucial for Methionine-Choline-Deficient Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice

Yin-Hua Zhang,De-Ping Ding,De-Qiang Ma,Juan Li,Lin-Li Chen,Kang-Jian Ao,You-You Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9

Purpose: To explore the influence of S100 calcium binding protein A4 (S100A4) knockout (KO) on methionine-choline-deficient(MCD) diet-induced non-alcoholic fatty liver disease (NAFLD) in mice. Materials and Methods: S100A4 KO mice (n=20) and their wild-type (WT) counterparts (n=20) were randomly divided into KO/MCD, Ko/methionine-choline-sufficient (MCS), WT/MCD, and WT/MCS groups. After 8 weeks of feeding, blood lipid and liverfunction-related indexes were measured. HE, Oil Red O, and Masson stainings were used to observe the changes of liver histopathology. Additionally, expressions of S100A4 and proinflammatory and profibrogenic cytokines were detected by qRT-PCR andWestern blot, while hepatocyte apoptosis was revealed by TUNEL staining. Results: Serum levels of aminotransferase, aspartate aminotransferase, triglyceride, and total cholesterol in mice were increasedafter 8-week MCD feeding, and hepatocytes performed varying balloon-like changes with increased inflammatory cell infiltrationand collagen fibers; however, these effects were improved in mice of KO/MCD group. Meanwhile, total NAFLD activity scoresand fibrosis were lower compared to WT+MCD group. Compared to WT/MCS group, S100A4 expression in liver tissue of WT/MCD group was enhanced. The expression of proinflammatory (TNF-α, IL-1β, IL-6) and profibrogenic cytokines (TGF-β1, COL1A1,α-SMA) in MCD-induced NAFLD mice were increased, as well as apoptotic index (AI). For MCD group, the expressions ofproinflammatory and profibrogenic cytokines and AI in KO mice were lower than those of WT mice. Conclusion: S100A4 was detected to be upregulated in NAFLD, while S100A4 KO alleviated liver fibrosis and inflammation, inaddition to inhibiting hepatocyte apoptosis.

Preparation, self-assembly and performance modulation of gold nanoparticles decorated ferrocene-containing hybrid block copolymer multifunctional materials

Jian-Guo Zhang,Xue-Yin Zhang,Hua Yu,Yan-Ling Luo,Feng Xu,Ya-Shao Chen 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.65 No.-

Gold nanoparticles decorated stimuli-responsive copolymer hybrids, poly(2-methacryloyloxyethyl ferrocenecarboxylate)-block-poly(N-isopropylacryamide) decorated with gold nanoparticles, were synthesized through two-step successive RAFT and ensuing in-situ reduction. The hybrids could self-assemble into interesting micelle structures from globular, wormlike to rodlike shapes by altering the quality and compositions of solvents and ionic strength, and exhibited multifunctionality including quasi-reversible electrochemical behavior, redox-stress responsiveness and temperature sensitivity. The physicochemical and electrochemical properties were modulated by tailor-making the system compositions and redox reaction. The copolymer hybrids were expected to broaden their applications in nanobiomedicine including targeted drug carriers and magnetic resonance imaging, optical, electrochemical catalyst, optoelectronics and sensors etc.

Lifetime data modelling and reliability analysis based on modified weibull extension distribution and Bayesian approach

Yuan-Jian Yang,Wenhe Wang,Xin-Yin Zhang,Ya-Lan Xiong,Gui-Hua Wang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.11

Satellite reliability has long been a critical issue in space industry. In-orbit lifetime data modelling and the corresponding reliability analysis are indispensable aspects for satellite reliability. However, limited by available in-orbit lifetime data, these two aspects have not been well studied. Aiming to mitigate this limitation, we conduct a coherent research on satellite reliability analysis based on an in-orbit database of 1584 satellites. A nonparametric reliability analysis is firstly implemented on the data set to identify the characteristics of failure rate. Then, the modified Weibull extension distribution is used to model the satellite reliability. Point estimation and interval estimation are carried out by utilizing a Bayesian method. Posterior distributions of model parameters are obtained and reserved for further reliability analysis of newly launched satellites. The results presented in this paper can provide helpful information to the space industry for design improvement and test planning of new satellites.

억압, 해방 혹은 팽창

양건화 ( Jian Hua Yang ),조곤 ( Kun Zhao ),윤민 ( Min Yin ) 한국철학사연구회 2015 한국 철학논집 Vol.0 No.44

본 논문은 뉴미디어 시리즈 게임을 연구대상으로 하여 프로이드의 정신분석학과 매슬로우의 심리학을 주요 방법론으로 삼아 뉴미디어 시리즈게임의 심리적 기능을 논의하였다. 아울러 현대사회에서 후현대사회로의 변화에 따라, 또한 컴퓨터 기술의 발전과 갱신으로 뉴미디어 시리즈게임을 포함한 게임류가 매우 용이하게 심리적 기능에 영향을 준다는 것이다. 즉 억압, 해방, 팽창이다. 여기에는 후현대사회의 파편화와 욕망화의 특징이 있을 뿐만 아니라, 동시에 컴퓨터 기술의 갱신에 따른 더 나은 게임 체험을 할 수 있기 때문이다. 이 모든 것들은 게임 체험자의 심리적 기능에 영향을 주는 매우 중요한 요인이다. This paper considers new media serious games as the study object, and Freudian psychology and Maslow``s psychics theory as methodology. Research purpose of this paper is study psychological function for new media serious games, especially with the post-modern society is gradually into the modern society and updating of computer technology, the games which including new media serious games is extremely to cause depression, release and swelling. Fragmentation and desire are the feature of post-modern society, while also updating computer technology brings a powerful gaming experience features, these are important factors which formed the psychological function of gaming experiencer.

BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells

Yan Hua Gong,Jing Wu,Hua Qiang,Ben Liu,Zhi Kai Chi,Tao Chen,Bin Yin,Xiao Zhong Peng,Jian Gang Yuan 한국생화학분자생물학회 2008 BMB Reports Vol.41 No.4

AN EXTREMAL PROBLEM ON POTENTIALLY $K_{r,r}$-ke-GRAPHIC SEQUENCES

Chen, Gang,Yin, Jian-Hua The Korean Society for Computational and Applied M 2009 Journal of applied mathematics & informatics Vol.27 No.1

For $1{\leq}k{\leq}r$, let ${\sigma}$($K_{r,r}$ - ke, n) be the smallest even integer such that every n-term graphic sequence ${\pi}$ = ($d_1$, $d_2$, ..., $d_n$) with term sum ${\sigma}({\pi})$ = $d_1$ + $d_2$ + ${\cdots}$ + $d_n\;{\geq}\;{\sigma}$($K_{r,r}$ - ke, n) has a realization G containing $K_{r,r}$ - ke as a subgraph, where $K_{r,r}$ - ke is the graph obtained from the $r\;{\times}\;r$ complete bipartite graph $K_{r,r}$ by deleting k edges which form a matching. In this paper, we determine ${\sigma}$($K_{r,r}$ - ke, n) for even $r\;({\geq}4)$ and $n{\geq}7r^2+{\frac{1}{2}}r-22$ and for odd r (${\geq}5$) and $n{\geq}7r^2+9r-26$.

An extremal problem on potentially Kr,r-ke-graphic sequences

Gang Chen,Jian-Hua Yin 한국전산응용수학회 2009 Journal of applied mathematics & informatics Vol.27 No.1

For 1≤ k≤ r, let σ(Kr,r −ke, n) be the smallest even integer such that every n-term graphic sequence π= (d1, d2, . . . , dn) with term sum σ(π) = d1 + d2 + · · · + dn ≥ (Kr,r − ke, n) has a realization G containingKr,r − ke as a subgraph, where Kr,r − ke is the graph obtained from the r × r complete bipartite graph Kr,r by deleting k edges which form a matching. In this paper, we determine σ(Kr,r − ke, n) for even r (≥4) and n 7r2 + ½ r − 22 and for odd r (≥5) and n≥ 7r2 + 9r −26. For 1≤ k≤ r, let σ(Kr,r −ke, n) be the smallest even integer such that every n-term graphic sequence π= (d1, d2, . . . , dn) with term sum σ(π) = d1 + d2 + · · · + dn ≥ (Kr,r − ke, n) has a realization G containingKr,r − ke as a subgraph, where Kr,r − ke is the graph obtained from the r × r complete bipartite graph Kr,r by deleting k edges which form a matching. In this paper, we determine σ(Kr,r − ke, n) for even r (≥4) and n 7r2 + ½ r − 22 and for odd r (≥5) and n≥ 7r2 + 9r −26.

ZNF488 Enhances the Invasion and Tumorigenesis in Nasopharyngeal Carcinoma Via the Wnt Signaling Pathway Involving Epithelial Mesenchymal Transition

Dan Zong,Li Yin,Qian Zhong,Wen-jie Guo,Jian-hua Xu,Ning Jiang,Zhi-rui Lin,Man-zhi Li,Ping Han,Lin Xu,Xia He,Mu-sheng Zeng 대한암학회 2016 Cancer Research and Treatment Vol.48 No.1

Purpose The purpose of this study was to investigate the function of Zinc finger protein 488 (ZNF488) in nasopharyngeal carcinoma (NPC). Materials and Methods The endogenous expression of ZNF488 in NPC tissues, normal nasopharyngeal epithelium tissues and NPC cell lines were detected by quantitative reverse transcription polymerase chain reaction. ZNF488 over-expressing and knock-down NPC cell line models were estab- lished through retroviral vector pMSCV mediated over-expression and small interfering RNA (siRNA) mediated knock-down. The invasion and migration capacities were evaluated by wound healing and transwell invasion assays in ZNF488 over-expressing and control cell lines. Soft-agar colony formation and a xenograft experiment were performed to study tumorigenic ability in vitro and in vivo. Immunofluorescence and western blotting analysis were used to examine protein changes followed by ZNF488 over-expression. Microarray analysis was performed to explore gene expression profilings, while luciferase reporter assay to evaluate the transcriptive activity of Tcf/Lef. Results ZNF488 was over-expressed in NPC tissues compared with normal tissues, especially higher in 5-8F and S18, which are well-established high metastatic NPC clones. Functional studies indicate that over-expression of ZNF488 provokes invasion, whereas knock-down of ZNF488 alleviates invasive capability. Moreover, over-expression of ZNF488 promotes NPC tumor growth both in vitro and in vivo. Our data further show that over-expression of ZNF488 induces epithelial mesenchymal transition (EMT) by activating the WNT/β -catenin signaling pathway. Conclusion Our data strongly suggest that ZNF488 acts as an oncogene, promoting invasion and tumorigenesis by activating the Wnt/β -catenin pathway to induce EMT in NPC.