Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

여수국가산업단지 인근 지역 대기오염물질 농도에 영향을 미치는 요인분석

김민영,변정훈,이영훈,이건영,한지은,이예림,임지현,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Eight monitoring stations in the Yeosu National Industrial Complex will determine the concentration of air pollutants measured every hour in 2019, analyze what factors affect them, and how they correlate with the air pollutant measurements. Six air pollutants (SO2, NO2, O3, CO, PM-10 and PM-2.5) measured at eight monitoring stations around Yeosu National Industrial Complex (Seogang-dong, Wolnae-dong, Munsu-dong, Yeocheon-dong, Deokchung-dong, Hwayang-myeon, Yulchon-myeon, Samil-dong) the measurement data for each hour of the year were downloaded to analyze the concentration by weather condition, time zone, season, separation distance from Yeosu Industrial Complex, and separation distance from Gwangyang Industrial Complex. SO2, NO2, and CO concentrations were significantly higher in winter, significantly lower in summer, and O3, PM-10, and PM-2.5 concentrations were significantly higher in spring. SO2, CO, PM-10, and PM-2.5 concentrations were significantly higher between 10 a.m. and 12 p.m., NO2 concentrations were significantly higher between 7 a.m. and 9 a.m., and O3 concentrations were significantly higher between 13 p.m. and 15 p.m.(p<0.001). The concentration of air pollutants (SO2, NO2, CO, PM-10, PM-2.5) excluding O3 was found to have a negative (-) correlation with weather condition, and in the case of O3, it was found to have a positive (+) correlation with PM-10, PM-2.5, temperature and wind speed. It was confirmed that the concentration of air pollutants emitted from the Yeosu National Industrial Complex differs depending on the season, time and distance to the industrial complex. This study aims to establish measures to minimize health damage to residents living relatively close to the industrial complex and contribute to the establishment of management and response measures according to the properties of substances to reduce the concentration of air pollutants in the future.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

점포내 VMD요소가 SPA 브랜드 정체성에 미치는 영향

김소영, 손지훈, 심효진, 신동훈, 정일호, 홍동우, 김은영 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

This study was to examine the effect of VMD factors on brand identity for SPA brands. As brand stimulus, four SPA brands(e.g., Basic House, Codes combine, Uniqlo, Zara) were selected by pretest. For collecting data, a self-administered questionnaire was developed based on the literatures and intercept survey was conducted. A total 508 usable responses were obtained from consumers who shopped at the selected brands' stores. For data analysis, descriptive statistics, factor analysis, and regression analysis were used. As perceived by consumers, VMD consisted of seven factors: lightening, scent, color, layout, music, window display, and item presentation. The VMD factor was significantly related to brand identity for the SPA brands. Specifically, brand awareness was predicted by color, layout and item presentation; brand association strength was predicted by color, layout, and widow display; favorable brand association was predicted by item presentation; salience of brand association was predicted by color and music; and brand image association was predicted by color, layout, music, and item presentation. Thus, this study discussed a managerial implication for SPA retailers to develop a visual merchandising strategy in the competitive marketplace.

영상처리에 의한 버스전용차로 위반차량의 검출방법

김지영 서원대학교 응용과학연구소 2001 응용과학연구 Vol.10 No.1

In this paper, an image processing method detecting the vehicles which violate the exclusive bus lain traffic regulation is proposed. The tow cameras are used to capture the images of the first lain of a highway where the exclusive bus lain traffic regulation is enforced. One camera is used to capture the front view of a vehicle, and the second camera to capture the side view of the craft. The front view camera is used for recognizing the vehicle licence number. The side view camera is employed for categorizing the vehicle class. The proposed method in this paper is very practical to be implemented because it does not need any attachment on the vehicle such as an identification transmitter or any other electronic devices.

자유연애의 이상과 식민지 근대의 현실 : 김동인의 「약한 자의 슬픔」 연구

김지영 우리어문학회 2003 우리어문연구 Vol.20 No.-

The acclamation for free marriage and free love was an important constituent of the process of attempting to construct the institutional emotion and family structure newly modeling the modern western romantic love. Until the late 1910s, the idea of free marriage and free love was too strongly tied with the ideology of enlightenment, thus only part of the meaning, the freedom to choose a marital partner, was stressed. Moreover, marriage was strongly tied up with the will to devote toward the nation, so choosing a marital partner was on the will to be a good people for the nation rather than on natural feeling of love. Free love started to be described in its genuine meaning from the works of Kim Tongin and his colleagues of the literary magazine "Creation", though the meaning of it was still unstable and on the way of changing. "The Sorrow of the Weak" showed the inauthenticity of the concept of free love by describing a woman's inner inconsistence and the ordeals that happened in her life while she aspired to romantic love. Kim, the author, showed the leftovers of pre-modern ethic inside a woman of the time who was seeking modern life and revealed the abstractness of her admiration for romantic love. We can find the contradiction between pre-modern and modern love ethics and the conflict between physical and spiritual love in the protagonist's interior. The falsehood, disorganization, and inversion of love revealed by Elizabeth while she was chasing after romantic love and modern life were not different from the falsehood, disorganization, and inversion of the idea of free love of the period itself. This reflects one of the colonial characteristics of Korean modernization that was considered as the most urgent and transcendental aim by many Korean enlighteners of the day.

신경인성 방광 1례에 대한 임상고찰

김만호,이지영,이정원,조충식,김철중 대전대학교 한방병원 2002 惠和醫學 Vol.11 No.1

From January 10th 2002 to February 17th 2002, the clinical study was carried out I case of patient in trouble with voiding difficulty such as urinary incontinence, enuresis and urinary frequency after the L₄-L_(5) HN P operation, who had been hospitalised in the Dept. of Internal Medicin e, Cheon-An Oriental Medical Hospital, Daejeon University. The results were as following 1. For this case, we could get the better result using Bo-Joong-lk-Gi-Tang(補中益氣湯) in the treating period. 2. We could get the effective result that the case of voiding difficulty after HNP operation and cerebral infarction by conservative therapy of oriental medicine such as acupuncture, moxibustion and herb medicine.

남녀 고등학생의 교복과 자율복의 대한 태도 연구

김미정,김영숙,배지혜,신영옥,장명희,황인순 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

The purpose of this study was to investigate the attitude difference toward the school uniform, it was supposed that clothing acceptance was differentiated according to subjects. The subjects were grouped by 3 types which were students, teachers(school governors) and parents groups. The results were as follows; 1. Boy students reguired the more freedom of clothing than girls. 2. Teachers and parents groups considered that clothing affected student's behavior and self-esteem. 3. The acceptance of school plain dress was affected by atomosphere of home and school. 4. According to socioeconomic levels, there were differences in clothing acceptance and fashion acceptance.

수학 학습 부진아의 인식 및 학습 실태 연구

김판수,조지영 부산교육대학교 교육대학원 2004 논문집 Vol.6 No.-

본 연구는 수학교과의 학습에 방해가 되고 있는 요인을 조사·분석함으로써 학생들의 수학 교과에 대한 부적응 상황을 진단하여 학생들의 수학 부진을 줄이고 나아가 교수-학습 개선에 도움을 주고자 하는데 목적을 둔다. 이를 위해 초등학교 5학년을 대상으로 우선 수학 학습 부진 학생들의 인식을 조사, 분석하였다. 또한 수학 학습 부진아를 지도할 때 학생들이 어려워하는 학습 내용을 사전에 파악하는 것이 학습부진을 줄일 수 있는 중요한 수단으로 보고 4학년 수학 단원 평가를 실시하여 이들이 어떤 내용을 잘 이해하지 못하는지를 분석하였다. 그 결과 수학 학습 부진 학생들은 수학 교과에 대해 부정적으로 생각하고 있었으며, 이들이 어려워하는 수학 내용은 다단계 사고를 요하는 개념, 논리적 연역적으로 추론을 요하는 개념, 문제해결이었다. 그 중에서도 특히 사고를 요하는 문제해결을 어려워하는 것으로 나타났다. This study sets out to investigate underachieved fifth graders in mathematics in terms of the understanding and learning state of mathematics and to help mathematics underachievers by providing make-up class. Thus we need to investigate the following: 1. An understanding of mathematics for underachieved students. 2. A research of the learning contents that the students have difficulties to deal with. For this study we first select 20 underachieved students(12 boys and 8 girls) in mathematics attending an elementary school located in Busan and then a questionnaire is distributed to see their understanding of mathematics. In addition, their performances are evaluated by using a checklist. The questionnaire contains total 30 items and each frequency and percentage are analyzed. The checklist contains 10 items of the contents of 4-Ga and 4-Na respectively. textbooks, The frequency and percentage are also analyzed. We have the following conclusion on mathematics underachievers. First, since they have a negative opinion of the subject, we need to increase the chances of their successful experiences so that they have their confidence and positive attitudes. Second, we should try to teach basic learning contents at the beginning of each unit thoroughly and focus on advanced learning contents gradually by intensifying and reinforcing the contents. Third, it is more desirable to give them suitable assignments they can handle rather than to force to teach advanced contents. An evaluation should be given every time after finishing a unit, and the results should be informed to students. And make-up class should be given if necessary.

YCbCr 공간에서의 컬러 영역화

김지영 서원대학교 미래창조연구소 2004 과학과 문화 Vol.1 No.1

In this paper a new color segmentation algorithm is proposed. The proposed algorithm uses YCbCr 3D color space where similar group of colors are easily identified. Two dimensional color space has difficulty in identifying group of colors while the processing time is surely advantageous. In the YCbCr histogram, those groups of colors are looked like stems and branches of a tree. A recursive region growing algorithm is developed and implemented in this paper. The proposed algorithm gives good results but the processing cost is very high. The spatial information of the image plane for the proposed region growing algorithm will be useful for the future research.