초등학교 과학과 멀티미디어 안전교육 자료 개발

정지웅,공영태,김성규 晋州敎育大學校 科學敎育硏究院 2006 科學敎育硏究 Vol.29 No.-

The purpose of this study was to develop safety instruction material that can use efficiently through analyze segments connected with safety in elementary science material field experiment. Therefore, established following assignment to achieve purpose of this study. First, analyze whether there are some things on hazardous factor that can be connected to safety accident at elementary science material field experiment. Second, develop multimedia material for effective safety instruction. Third, recognize teacher's legal liability through published paper and case study. To settle the above subject, secures theoretical basis through literature study and collected datas connected with safety instruction through internet search. Also analyzed risk factor and safety connection contents that can be connected to safety accident through the 7th elementary science textbooks. Then arrange this systematically and made a story board. Safety connection animation datas that film by camcorder edited by vegas 4.0 and used Macromedia company's Director MX developing multimedia material. before scientific experiment class beginning or when educate safety, divide into greatly 6 low rank menus to use easily necessary member and arranged connection menus inside. All materials were manufactured to use easily maximum for user. This material will prevent beforehand accident to occur because make an experimenter know enough safety precaution, safety equipment, experiment tool directions etc. when safety accident occurred, will help unconnect to large size accident because do quick emergency measure.

표면곡률을 이용한 물체의 형상특징추출

조동욱,김지영,박웅규,윤상균,이성석,김태우 西原大學校 1992 西原大學 論文集 Vol.29 No.-

In this paper, a new shape feature extraction method of the objects using surface curvatures is described. First, the values of the H & K are calculated from range data as given input. Then objects are assigned in the form of 8 primitives by the signs of the H & K values. But existing methods have the difficulties with the complex object. Therefore, We propose the method for the complex primitives such as cone ridge and cone valley. Finally, the effectiveness of this paper is demonstrated by several experiment.

가막사리(Bidens tripartita L.)와 미국가막사리(Bidens frondosa L.) 종자의 발아에 미치는 몇가지 요인

신혜정,신종섭,김지훈,김학윤,이인중,신동현,김길웅 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

The experiments were conducted to determine the factors such as light and darkness, phytohormone and seed coat, influencing on seed germination of Bidens tripartita L. and B. frondosa. The seeds of both species were germinated when seed coat was damaged and weakened GA3 and BA stimulated germination of both species but ABA and IAA had no effect on germination of them, which ranged 50.0% to 80.0%. In B. forndosa, when inner layer of seed coat was removed, germination was highly promoted up to 96.7% compared with 10.0% germination rate in another treatments.

The metabolic mechanism of C-14 labeled chitosan in mice

Kim, Kwang Yoon,Kim, Young Ho,Kim, Hee Kyung,Bom, Hee Seung,Kim, Ji Yeul,Yoshikazu Nishimura,Jkim, ung Woo,Oh, Chang Suck,Park, Ro Dong,Lee, Hyun Chul,Kang, Moon Il,Roh, Young Bok 한국키틴키토산학회 1998 한국키틴키토산학회지 Vol.3 No.1

Chitin is exists in the exoskeleton of crustaceans and chitosan can be obtained by deacetylation of chitin. As chitosan has a chelating characteristics, it was used radiostrontium chelator in the rats and mice. It also can be used as a healthy food and medicine. The purpose of the prsent study is to investigate the chitosan distribution and excretory route of 14C-chitosan in the animal body. 14C-chitosan was prepared and intravenously administered to the vein of mice and also intraorally ingested. The body distribution of chitosan was studied by autoradiography and the urinary excretion was counted. C-14 label chitosan was showed high distribution in the liver. And 10~20% of chitosan was excreted to the urine through kidney within few days. Chitosan was gathered into gromerulus in kidney and excreted from three hours. It was concluded that chitosan has no target organs and liver distribution is a sort of only passing route for the urinary excretion by way of kidney.

CoFeSiB–Pd multilayers and co-deposited alloy films exhibiting perpendicular magnetic anisotropies after heat treatment up to 500 °C

Kim, Yong Jin,Kim, Seung Hyun,Kim, Ki Ha,Kim, Do Kyun,Cho, Ji Ung,Kim, Kyou-Hyun,Kim, Young Keun Elsevier 2017 ACTA MATERIALIA Vol.125 No.-

<P>Ferromagnetic layers exhibiting perpendicular magnetic anisotropy (PMA) are essential components in magnetic random access memory cells. Various Co- and Fe-based multilayers (MLs) have been considered because the magnetic properties of MLs can be easily controlled by changing the ferromagnetic and nonmagnetic layer thicknesses and number of bilayers. However, MLs generally suffer from the loss of PMA after high-temperature heat treatments (beyond approximately 400 degrees C) during device processing, which are associated with back-end-of-line device fabrication. Moreover, ML deposition may limit the throughput of the film mass production. Here, we investigate the PMA stability of sputter-deposited CoFeSiB/Pd ML as well as CoFeSiB-Pd co-deposited alloy (CA) films exposed to various annealing temperatures. Although the ML films exhibited PMA, regardless of the annealing temperature up to 500 degrees C, the CA films started to exhibit strong PMA after 400 degrees C, which was maintained up to 500 degrees C. To understand the magnetic property changes, we performed microstructural analyses. The ML films in the as deposited state exhibited well-defined layered microstructures and evolved into polycrystalline alloys at 500 degrees C. In contrast, the CA films in the as-deposited state exhibited an amorphous microstructure but transformed into polycrystalline alloys at 500 degrees C, similar to their ML counterparts. Both Co and Pd, the major constituents of the films, underwent interdiffusion and formed a Co-Pd ordered phase, which contributed to the appearance of PMA. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</P>

Dendritic Cell Dysfunction in Patients with End-stage Renal Disease

Kim, Ji Ung,Kim, Miyeon,Kim, Sinae,Nguyen, Tam Thanh,Kim, Eunhye,Lee, Siyoung,Kim, Soohyun,Kim, Hyunwoo 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>End-stage renal disease (ESRD) with immune disorder involves complex interactions between the innate and adaptive immune responses. ESRD is associated with various alterations in immune function such as a reduction in polymorphonuclear leukocyte bactericidal activity, a suppression of lymphocyte proliferative response to stimuli, and a malfunction of cell-mediated immunity at the molecular level. ESRD also increases patients' propensity for infections and malignancies as well as causing a diminished response to vaccination. Several factors influence the immunodeficiency in patients with ESRD, including uremic toxins, malnutrition, chronic inflammation, and the therapeutic dialysis modality. The alteration of T-cell function in ESRD has been considered to be a major factor underlying the impaired adaptive cellular immunity in these patients. However, cumulative evidence has suggested that the immune defect in ESRD can be caused by an Ag-presenting dendritic cell (DC) dysfunction in addition to a T-cell defect. It has been reported that ESRD has a deleterious effect on DCs both in terms of their number and function, although the precise mechanism by which DC function becomes altered in these patients is unclear. In this review, we discuss the effects of ESRD on the number and function of DCs and propose a possible molecular mechanism for DC dysfunction. We also address therapeutic approaches to improve immune function by optimally activating DCs in patients with ESRD.</P>

2018년도 국제기술혁신협력사업

김왕동(Wangdong Kim),송치웅(Chi Ung Song),이정원(Jung Won Lee),임덕순(Deok Soon Yim),김기국(Kikook Kim),김은주(Eun Joo Kim),권소현(So Hyun Kwon),김민정(Min Jung Kim),김성아(Sung A Kim),김지선(Ji Sun Kim),안지용(Ji Yong An),전병우(Byungw 과학기술정책연구원 2018 조사연구 Vol.- No.-

Structural and expression analysis of prolamin genes in Oryza sativa L.

Ung-Han Yoon,Tae-Ho Kim,Jeonghwa Lee,JangHo Hahn,Yeon-Ki Kim,Gang-Seob Lee,Hyeon-So Ji,ChangKug Kim,문정환,Young Mi Kim 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3

Rice is a staple crop with a small genome of 389 Mb. Rice grain is a source of carbohydrates and proteins and has a relatively low protein content compared to other legume seeds. Glutelin and prolamin are the major storage proteins in rice. Prolamins are characterized by high glutamine and proline content and are generally soluble only in strong alcohol solutions. In this study, we obtained a total of 51,383 expressed sequence tags (ESTs)from Ilpumbyeo (Oryza sativa L.), of which 33,201 and 18,182 clones were obtained from immature and germinating seeds, respectively. From the EST clones, 15,148unigenes were identified, and 2,590 genes were expressed in both immature and germinating seeds. Gene expression profiling of rice prolamins indicated that prolamin gene expression increased 5 days after heading and reached maximal expression after 30 days, suggesting a high demand for prolamins during seed development and germination. Phylogenetic analysis grouped 33 prolamin genes based on the abundance of sulfur-containing amino acids methionine and cysteine according to the deduced amino acid sequences. Our results enhance the understanding of the regulation of seed maturation and germination, which can result in improved agricultural traits for the seed industry.

Protein tyrosine phosphatase conjugated with a novel transdermal delivery peptide, astrotactin 1–derived peptide recombinant protein tyrosine phosphatase (AP-rPTP), alleviates both atopic dermatitis–like and psoriasis-like dermatitis

Kim, Won-Ju,Koo, Ja-Hyun,Cho, Hyun-Jung,Lee, Jae-Ung,Kim, Ji Yun,Lee, Hong-Gyun,Lee, Sohee,Kim, Jong Hoon,Oh, Mi Seon,Suh, Minah,Shin, Eui-Cheol,Ko, Joo Yeon,Sohn, Myung Hyun,Choi, Je-Min Mosby 2018 The journal of allergy and clinical immunology Vol.141 No.1

<P><B>Background</B></P> <P>Atopic dermatitis (AD) and psoriasis are the 2 most common chronic inflammatory skin diseases. There is an unmet medical need to overcome limitations for transcutaneous drug development posed by the skin barrier.</P> <P><B>Objective</B></P> <P>We aimed to identify a novel transdermal delivery peptide and to develop a transcutaneously applicable immunomodulatory protein for treating AD and psoriasis.</P> <P><B>Methods</B></P> <P>We identified and generated reporter proteins conjugated to astrotactin 1–derived peptide (AP), a novel transdermal delivery peptide of human origin, and analyzed the intracellular delivery efficiency of these proteins in mouse and human skin cells and tissues using multiphoton confocal microscopy. We also generated a recombinant therapeutic protein, AP–recombinant protein tyrosine phosphatase (rPTP), consisting of the phosphatase domain of the T-cell protein tyrosine phosphatase conjugated to AP. The immunomodulatory function of AP-rPTP was confirmed in splenocytes on cytokine stimulation and T-cell receptor stimulation. Finally, we confirmed the <I>in vivo</I> efficacy of AP-rPTP transdermal delivery in patients with oxazolone-induced contact hypersensitivity, ovalbumin-induced AD-like, and imiquimod-induced psoriasis-like skin inflammation models.</P> <P><B>Results</B></P> <P>AP-conjugated reporter proteins exhibited significant intracellular transduction efficacy in keratinocytes, fibroblasts, and immune cells. In addition, transcutaneous administration of AP-dTomato resulted in significant localization into the dermis and epidermis in both mouse and human skin. AP-rPTP inhibited phosphorylated signal transducer and activator of transcription (STAT) 1, STAT3, and STAT6 in splenocytes and also regulated T-cell activation and proliferation. Transcutaneous administration of AP-rPTP through the paper-patch technique significantly ameliorated skin tissue thickening, inflammation, and cytokine expression in both AD-like and psoriasis-like dermatitis models.</P> <P><B>Conclusion</B></P> <P>We identified a 9-amino-acid novel transdermal delivery peptide, AP, and demonstrated its feasibility for transcutaneous biologic drug development. Moreover, AP-rPTP is a novel immunomodulatory drug candidate for human dermatitis.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Inactivation of the CTD phosphatase-like gene <i>OsCPL1</i> enhances the development of the abscission layer and seed shattering in rice

Ji, Hyeonso,Kim, Sung-Ryul,Kim, Yul-Ho,Kim, Hakbum,Eun, Moo-Young,Jin, Il-Doo,Cha, Young-Soon,Yun, Doh-Won,Ahn, Byung-Ohg,Lee, Myung Chul,Lee, Gang-Seob,Yoon, Ung-Han,Lee, Jung-Sook,Lee, Yeon-Hee,Suh, Blackwell Publishing Ltd 2010 The Plant journal Vol.61 No.1

<P>Summary</P><P>Although susceptibility to seed shattering causes severe yield loss during cereal crop harvest, it is an adaptive trait for seed dispersal in wild plants. We previously identified a recessive shattering locus, <I>sh-h</I>, from the rice shattering mutant line Hsh that carries an enhanced abscission layer. Here, we further mapped <I>sh-h</I> to a 34-kb region on chromosome 7 by analyzing 240 F<SUB>2</SUB> plants and five F<SUB>3</SUB> lines from the cross between Hsh and Blue&Gundil. Hsh had a point mutation at the 3′ splice site of the seventh intron within LOC_Os07g10690, causing a 15-bp deletion of its mRNA as a result of altered splicing. Two transferred DNA (T-DNA) insertion mutants and one point mutant exhibited the enhanced shattering phenotype, confirming that LOC_Os07g10690 is indeed the <I>sh-h</I> gene. RNA interference (RNAi) transgenic lines with suppressed expression of this gene exhibited greater shattering. This gene, which encodes a protein containing a conserved carboxy-terminal domain (CTD) phosphatase domain, was named <I>Oryza sativa CTD phosphatase-like 1</I> (<I>OsCPL1</I>). Subcellular localization and biochemical analysis revealed that the OsCPL1 protein is a nuclear phosphatase, a common characteristic of metazoan CTD phosphatases involved in cell differentiation. These results demonstrate that OsCPL1 represses differentiation of the abscission layer during panicle development.</P>