Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

Two cases of Bowen`s disease successfully treated with ingenol mebutate (PICATO®)

( Je Byeong Chae ),( Jung Tae Park ),( Jung Won Shin ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Bowen’s disease is an intra-epidermal squamous cell carcinoma in situ of skin. When surgical approach is unaffordable, nonsurgical therapies (e.g. topical agents) can provide alternative treatment options. Ingenol mebutate is a novel topical agent extracted from Euphorbia peplus, which has been approved by the FDA for the field therapy in actinic keratosis. We report two cases of Bowen’s disease successfully treated with ingenol mebutate gel. The first case is a 68 year old woman woman presented with gray-to-red irregular shaped plaque on her shin. Histopathologic findings were consistent with Bowen’s disease. The patient was treated with 0.05% ingenol mebutate gel combined with CO2 fractional resurfacing laser. Follow-up skin biopsy revealed no evidence of remnant lesion. The second case is an 82 year old woman with Bowen’s disease on her left cheek. Even after four times of cryotherapy the lesion was not completely removed. After applying 0.015% ingenol mebutate, the lesion was cleared and no recurrence was observed after one year. Until now, there have been few reports demonstrating the efficacy of ingenol mebutate for the treatment of BD. We suggest that ingenol mebutate might useful and effective treatment option for BD, leading to optimal cosmetic result.

테니스 양손 백핸드 스트록의 3차원 운동학적 분석

신제민 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to analysis 3-d kinematic characteristics of two hand backhand stroke in tennis, 3 skilled subjects who were performed 3 successful trials were filmed with three dimensional cinematography. Kinematic variables computed were three dimensional displacements of racquet and angles of joints participated in the stroke. The 3-d angles were calculated by vector projected with the least squares solution in order to de fine complex joint movements, specially shoulder joints. The following conclusion were reached; 1. The direction of racquet at the impact was reverse to the aimed direction of a ball in the transverse plane. The role of trajectory of racquet after impact was also important to decide the ultimate direction of ball. 2. The angular motion in the horizontal adduction/abduction of the shoulder joints was affected to axial rotation of trunk the angle of internal/external rotation in the shoulder joints increased around the impact without increasing until the impact. 3. The angles of elbows joint were flexed below 150˚ at the impact, not fully extending. 4. The angle of internal/external rotation of trunk was external of trunk was rotated over 90˚ at the impact while insignificant angular oration in the other planes. 5. The pattern of ante of knee joints showed variability within subjects and trials without typical angular pattern during the total movement phase. But the magnitude and time of peak flexion were appeared before the impact at the left bide. 6. Both of foots were closed over 180˚ from the base line among local as at the impact. The right foot was much closed than left foot at impact as well as all of the phase, and almost keep fixed hat changing the angle until the impact.

간질발작을 주 증상으로 하는 회백질 이소증을 동반한 Schizencephaly 1례

신석호,양일권,김제헌 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.4

We experienced a case of schizencephaly with heterotopic gray matter that was only manifested a seizure attack. The clinical manifestation of this patient was only seizure without mental retardation, which was controlled by anticonvulsants. The term schizencephaly was introduced by Yakovlev and Wadsworth in 1946 to describe bilatral, nearly symmetrical and full-thickness clefts, which were characterized by an infolding of cortical gray matter along the clefts. The most widely accepted theory for the pathogenesis of this anomaly is the failure of formation of a segment of the germinal matrix or segmental failure of neuronal migration. Heterotopic gray matter is frequently found within and near the cleft. Patients who have these condition present clinically with seizures and developmental delay, and abnormal motor skills are noted in the more severly affected cases. Recognition is important for planning proper treatment and for genetic counseling.

다구치법에 의한 고 규소 내산주철의 용해주조공정 최적화

신제식,이상목,문병문,김정철,한동운,백승한 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

In this study, Taguchi method, one of the design tools of experiments, was applied to optimize casting process parameters of the high silicon corrosion-resistant iron. Three casting process parameters such as melting temperature, rare earth metal (mischmetal) addition, and pouring temperature were chosen. Using SN (signal-to-noise) ratio calculated from ultimate tensile strength of each experimental casting run, the relationship between the casting parameters and mechanical properties was statistically evaluated. The casting condition of a melting temperature of 1,650℃, 0.2% rare earth metal addition, and a pouring temperature of 1,350℃ led to an excellent ultimate tensile strength above 11 kgf/mm², which is beyond the industrial criterion. The effects of the casting parameters on mechanical properties and corrosion resistance were further confirmed by combined analysis of fractography, hydrogen content determination, microscopic test, and acid resistance test. (Received August 23, 2004)

급성 외상성 기관 및 기관지손상의 치험 1 예

신제균,정종필,홍은석 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Recently, increased traffic accident has resulted in a corresponding increase in incidences of blunt trauma. Tracheal and main bronchial injuries after blunt chest trauma can be life-threatening, and hence early diagnosis and early management of patients with such injuries are essential. A 3-year-old boy was transferred to the Department of Emergency Medicine after a motor vehicle accident in which he sustained multiple blunt injuries consisting of tracheal rupture, left main bronchial rupture, liver laceration, vitreous hemorrhage, and fracture of the iliac wing. The tracheal rupture was managed nonoperatively with intensive respiratory care, while the left main bronchial rupture was managed surgically by bronchoplasty. Postoperative course was uneventful and the patient was discharged without respiratory problems.

급성 림프구성 백혈병 환자에서 발생한 침습성 국균 심내막염 2예

신성철,백경란,정재홍,송봉근,김동희,전경만,채제욱,손준성,정숙인,오원섭,송재훈 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

국균은 호흡기를 통해 유입되며 심한 면역기능저하 환자에서 기회감염을 일으키는 진균이며 주로 혈관을 따라 폐를 침범하고 심장과 골침범은 매우 드문 것으로 알려져 있다. 저자들은 급성 림프구성 백혈병 환자에서 발생한 침습성 국균 심내막염을 2예을 경험하였다. 19세 남자환자로 동종 조혈모세포이식 후 발생한 고열과 요통의 원인으로 감염성 심내막염 진단하에 수술결과 국균에 의한 감염으로 확증되어 수술적 절제술과 liposomal amphotericin B로 치료하였으나 국균 심내막염의 재발과 국균 척추염의 악화, 뇌 출혈로 사망하였다. 23세 남자환자로 관해요법 후 발생한 고열과 요통의 원인으로 국균 심내막염과 국균 척추염 진단하에 수술적 절제술과 amphotericin B로 치료하여 1년 2개월 동안 국균 심내막염의 재발은 없었으나 지속적인 백혈병의 재발과 국균 척추염의 악화, 침습성 폐 국균증으로 사망하였다. Although Aspergillus endocarditis has rarely been reported, it can cause fatal complications in hematologic malignancy patients and allogeneic stem cell transplant recipients. We experienced two cases of aspergillus endocarditis developed in acute lymphoblastic leukemia patients. Case; A 19-year-old patient developed Aspergillus endocarditis after allogenic hemopoietic stem cell transplantation. He was treated with surgical intervention and liposomal amphotericin B. He died of recurred Aspergillus endocarditis and cerebral hemorrhage probably related with aspergillosis of central nervous system. Case 2; A 23-year-old patient developed invasive Aspergillus endocarditis after induction chemotherapy. Aspergillus endocarditis was successfully treated by surgical intervention and amphotericin B. He died of refractory neutropenic fever and sepsis after the third relapse of leukemia and repetitive chemotherapy. He probably had invasive pulmonary aspergillosis without evidence of endocarditis recurrence. Because the mortality of Aspergillus endocarditis is very high, early diagnosis and surgical intervention are very important for better outcome.

태권도 옆차기와 돌려차기의 3차원 운동형태의 비교

신제민,진영완 한국운동역학회 2000 한국운동역학회지 Vol.9 No.2

The study compared to skills of Yeopchagi and Dolyeochagi in Taekwondo with the 3d kinematic analysis. To compare both of two skills, the study was analyzed as the relative time during the skills performed, the kinematic characteristics of individual segment, the relationship of inter-segment and the ranges of motion in 3d space. A performance time was mort shortened Dolyeochagi than Yeopchagi. A relative time that the time minimized angle of shank was later Dolyeochagi than Yeopchagi. The characteristic of kinematic of Individual segments showed clearly different in the flexion/extension plane. The angle of thigh increased continuously at an impact in Yeopchagi, but the angle stopped or decreased in Dolyeochagi at that time. The relationship of inter-segment showed difference largely between two skills in the flexion/extension plane, but the other plane showed no difference. These results were due to anatomical functional difference between two joints. The ranges of motion in 3d space were different at the abduction/adduction and the internal/external rotation.

膜分離에 의한 海水의 淡水化에 관한 硏究

申盛義,李性琪,崔炯一,申大允,金永範,李抵憲,姜永周 조선대학교 환경연구소 1989 環境公害硏究 Vol.6 No.-

In this study, we made investigation into water permeation, solute separation, concentration and Na^(+), K^(+), Mg^(2+), Ca^(2+), Cl^(-), SO^(2)_(4)-, HCO^(-)_(3) ions separation of seawater by the revers osmosis process using a suitable semipermeable membrane. The different thickness of membranes were prepared. On heat treatment, temperature was 80℃ and thier effects were also investigated. The flat single module system was made to be capable of treating feed solution at 200-2000cc/min and 120 atm. The experimental results can be summerized as follows: 1) As the effective operating pressure increased, permeability of pure water proportionally increased. 2) As the exposure period of the manufactured membrane in air increased, permeation rate increased, but as the thickness of the membrane increased, permeation rate decreased. 3) The capacity for being reproductive of the manufactured membrane (CA: 30wt%, Formamide: 45wt%, Acetone: 25wt%) in their performance was less than 10-30% and agreed with that reported in the literature. 4) We could separated about 85-95% of salt ion in seawater using a flat single module system.

남성화 암쥐의 모리스 수중 미로 수행력 : 성에 따른 공간기억력과 테스토르테론 효과의 차이

신성현,박제민,정우영,박문일,서유나,김성곤,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : It is widely accepted that gonadal steroids are involved in organization and activation of sexual dimorphism in the brain areas related with spatial leaming. This study aimed to test the following hypothesis : 1) spatial memory is affected by sex, 2) this difference is related with brain differentiation by exposure to testosterone in the neonatal period, and/or 3) the sexual difference of spatial memory is the result of the interaction between organization of sexual dimorphism in the brain and the circulating testosterone after growing up. Methods : Testosterone enanthate 100 ㎍, ('androgenized') orvehicle ('control') was randomly administered to 28 female and 25 male rat pups within 24 hour of birth. The escape latencies (EL) in Morris water maze were measured in 15 and 16 week of age with vehicle ('baseline') and with 10 μmol/kg of testosterone ('with testosterone'), respectively. Each session Consis-ted of 10 trials, and means of the last 5 trials were analyzed. Results : The baseline EL of the males were significantly shorter than females regardless ofneonatal exposure to testosterone. With testosterone, EL of the androgenized females were significantly shorter than control females and comparable to those of males. Conclusion : Neonatal exposure to testosterone in female rats may develop a tesosterone-dependent male-like spatial leaming system.