동물세포의 연속 대량생산에 관한 연구

이현용(Hyeon Y . Lee),강재구(Jae K . Kang),박형환(Hyung H . Park) 한국미생물생명공학회 1989 한국미생물·생명공학회지 Vol.17 No.3

Electron Cyclotron Resonance O_2 Plasma에서 증착한 규소 산화 박막의 특성

안명환,서문석,장재선,서성모,이기방,윤창주,이형재,남기석,최규현,손춘배,김용섭,강석희 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

규소 산화막을 ECR-CVD(electron cyclotron resonance-chemical vapor deposition) 증착방법으로 5인치 기판위에 상온에서 증착하고, 증착공정조건인 증착율, 기판온도, 마이크로파의 세기변화 및 플라즈마 혼합기체의 비에 따른 규소 산화막의 특성을 조사하였다. 또한 산화막의 구조적인 특성을 비교하기 위해 FTIR을 이용하여 ECR-CVD 증착한 산화막, RPE-CVD(remote plasma enhanced-CVD) 증착한 산화막 및 열 산화막의 stretching frequency를 측정하였다. 측정된 결과 ECR-CVD로 증착된 산화막이 구조적인 면에서 열 산화막과 거의 같음을 보였다. ECR-CVD로 증착된 산화막의 전기적인 특성을 전류-전압 및 축전-전압 측정에의하여 분석하였다. 축전된 산화막의 전기적인 특성은 산화막의 전하 밀도는 1×10 exp (11)/㎠이였고, 평균 절연 파괴 전압은 약 6 MV/㎝이다. We have grown thin films of SiO_2 at room temperature by using an ECR-CVD system and have investigated the changes in the properties of the deposited films with changes in the processing conditions such as the deposition rate, the substrate temperature, the microwave power and the plasma gas mixing ratio. We also measured the stretching frequency of three kinds of oxides, and ECR-CVD-grown oxide, a PECVD-grown oxide, and a thermally grown oxide, using FTIR analysis to compare their structural properties. The result shows that the structural properties of the ECR-grown oxide are similar to those of the thermally grown oxide. Additionally, the electrical properties of the ECR-grown oxide were investigated by using current-voltage and capacitance-voltage measurements. These electrical results indicate that the oxide charge density and the average breakdown voltage are 1×10 exp (11) ㎝^-2 and 6 MV/㎝, respectively.

전자기력에 의한 연주 Slab 내의 교반유동에 관한 수치적 연구

이재헌,이성우,신영길,김희창 대한금속재료학회(대한금속학회) 1990 대한금속·재료학회지 Vol.28 No.1

A Numerical study was performed for the two-dimensional turbulent stirring flow created by linear motor in a continuous casting system. A k-εturbulent model was applied to solve time-averaged turbulent momentum equations within the computation domain. Four different situations in a stirring system being consisted of two-dimensional slab of 1 m(width) × 0.2 m(height) have been investigated, that is : Model (A) : W =0.1 m and B_0=0.01 tesla, Model (B) : W =0.3 m and B_0=0.01 tesla, Model (C) : W =0.1 m and B_0=0.02 tesla, Model (D) : W =0.3 m and B_0=0.02 tesla, where W is the length of linear motor and B_0 is the magnetic flux applied on the motor-side-surface of the slab. The results shows that the flow characteristics depends mainly on the magnetude of B_0 not on W. The order of the magnitudes of turbulent intensity at four flow situations were almost same. However, the magnitude of turbulent viscosity became much less in case of greater B_0 than in smaller B_0. Therefore it is recommended that the stirrer with greater B_0 should be used to make finer stirring action.

T4 DNA Ligase 의 반응 양상에 대한 특성 연구

이현재,김훈,박무영 생화학분자생물학회 1991 BMB Reports Vol.19 No.3

The activity of T4 DNA ligase on cohesive ends as well as blunt ends of DNA fragments was studied. Optimum temperatures for joining DNA fragments digested by Eco RI, Pst I, Hind III, and Sma I were 6, 12, 6 and 24℃, respectively. Ligation efficiency of Eco RI-pUC 9 was higher than that of Hind III-pUC 9 and lower than that of Pst I-pUC 9 and very much higher than that of Sma I-pUC 9. The optimum concentrations of ATP for ligation of the two types of the substrates were different, 1.0 mM for cohesive ends and 0.2 mM for blunt ends. Both reactions were inhibited by monovalent cations, polyamines, phosphate and ethidium bromide. The ligation of blunt ends was more sensitive to these effectors except ethidium bromide than that of cohesive ends. The joining of blunt ends showed a non-linear dependency on enzyme concentration. Based on these results, the characteristic mode of action of T4 DNA ligase was suggested that the substrates are juxtapositioned by hydrogen bond for joining cohesive ends and the substrates are oriented by interaction between enzymes bound to DNA for blunt ends.

근거리 통신망 고장진단 전문가 시스템

최재영,이채영 한국경영과학회 1991 韓國經營科學會誌 Vol.16 No.1

As Expect system that diagnoses the malfunction of local area networks is developed. The system detects specific devices in the network as the source of the data disconnection. These sources are sct to goals in the knowledge base and rules and construct by including all possible occurrences UN connection of terminals and host computers. An approach via OR graph is employed for the systematic rule generation. The system is implemented in a shell and illustrative inference processes are presented.

신장암으로 전이된 암2예 보고

이상숙,노재윤 대한병리학회 1985 Journal of Pathology and Translational Medicine Vol.19 No.4

S. pneumoniae 호흡기감염 마우스에서 Rufloxacin의 항균력에 대한 조직학적 연구

구세광,이형식,김종대,최해윤,이재현 慶山大學校 基礎科學硏究所 2002 基礎科學 Vol.6 No.1

Streptococcus pneumoniae ATCC 6303을 이용한 국소 호흡기 감염 마우스 모델에서 Rufloxacin (RUFX)의 in vivo 항균력을 조직학적으로 평가하기 위하여 생균수와 폐의 조직·병리학적 변화를 관찰하였던 결과, 다음과 같은 결과를 얻었다. 1) 생균수는 RUFX 투여군에서 Control 군에 비해 유의성 있게 감소되었으며, 2). Control 군에서는 현저한 폐내 염증세포의 침윤, 출혈 및 폐포 벽의 비후가 조직학적으로 관찰되었으나, 이러한 조직학적 소견들은 RUFX 투여군에서 Control 군에 비해 현저히 있게 감소되었고, 3). LSA (luminal surface of alveoli %)는 Control 군에서 Sham 군에 비해 현저히 감소된 반면, RUFX 투여군에서 Control 군에 비해 유의성 있게 증가되었다. 이상에서 RUFX의 Sterptococcus pneumoniae에 대한 항균력이 조직학적으로 관찰되었다. In order to evaluate the in vivo effect of rufloxacin (RUFX), the viable bacterial number and histopathological changes were monitored after experimental respiratory infection with Sterptococcus pneumoniae ATCC 6303. In RUFX, the viable bacterial numbers were significantly and dose-dependently decreased compared to that of Control group. In Control group, severe infiltration of inflammatory cells, hemorrhage and hypertrophy of alveolar linings were demonstrated at microscopical levels. However, these abnormal histopathological changes were significantly and dose-dependently decreased compared to that of Control group in RUFX group. And also in RUFX group, the LSA (luminal surface of alveoli %) were significantly and dose-dependently increased compared to that of Control group. According to these results, it is considered as the in vivo antibacterial activity of RUFX was histologically showed against Steptococcus pneumoniae ATCC 6303 infection of respiratory tract in this study.

두 개의 이형성 결절에서 각각 동시에 발생한 간세포암종과 담관상피암종

권영미,이승규,김정선,노재윤,유은실 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.2

S. pneumoniae 호흡기감염 마우스에서 Ciprofloxacin의 항균력에 대한 조직학적 연구

구세광,이형식,김종대,최해윤,이재현 慶山大學校 基礎科學硏究所 2002 基礎科學 Vol.6 No.1

Sterptococcus pneumoniae ATCC 6303을 이용한 국소 호흡기 감염 마우스 모델에서 Ciprofloxacin (CPFX)의 in vivo 항균력을 조직학적으로 평가하기 위하여 생균수와 폐의 조직·병리학적 변화를 관찰하였던 결과, 다음과 같은 결과를 얻었다. 1) 생균수는 CPFX 투여군에서 Control 군에 비해 유의성 있게 감소되었으며, 2) Control 군에서는 현저한 폐내 염증세포의 침윤, 출혈 및 폐포 벽의 비후가 조직학적으로 관찰되었으나, 이러한 조직학적 소견들은 CPFX 투여군에서 Control 군에 비해 현저히 있게 감소되었고, 3) LSA (luminal surface of alveoli %)는 Control 군에서 Sham 군에 비해 현저히 감소된 반면, CPFX 투여군에서 Control 군에 비해 유의성 있게 증가되었다. 이상에서 본 실험의 결과, CPFX의 Sterptococcus pneumoniae에 대한 항균력이 조직학적으로 관찰되었다. In order to evaluate the in vivo effect of ciprofloxacin (CPFX), the viable bacterial number and histopathological changes were monitored after experimental respiratory infection with Sterptococcus pneumoniae ATCC 6303. In CPFX, the viable bacterial numbers were significantly and dose-dependently decreased compared to that of Control group. In Control group, severe infiltration of inflammatory cells, hemorrhage and hypertrophy of alveolar linings were demonstrated at microscopical levels. However, these abnormal histopathological changes were significantly and dose-dependently decreased compared to that of Control group in CPFX group. And also in CPFX group, the LSA (luminal surface of alveoli %) were significantly and dose-dependently increased compared to that of Control group. According to these results, it is considered as the in vivo antibacterial activity of CPFX was histologically showed against Sterptococcus pneumoniae ATCC 6303 infection of respiratory tract in this study.

한국연안 이상고수온과 저수온의 지속성 기간의 정량화

서영상,황재동,장이현,강용균 한국환경과학회 2001 한국환경과학회지 Vol.10 No.2

The magnitudes of sea surface temperature (SST) anomalies at 13 coastal stations along the Korean peninsula in the summer and winter for the past 29years (1969-1997) are more larger than those in the spring and autumn. The periods of positive SST anomalies (negative SST anomalies) longer than 1℃ were 75(74.5) months in the eastern coast of Korea, 47.8(51.6) months in the southern coast of Korea and 69.5(69.8) months in the western coast of Korea during the past 348 months (1969-1997). The predominant periods of the low-pass filtered monthly SST anomalies are 3 years or 13 months, even another predominant period is 24 months. The spatial variation of SST anomalies were confined by regional seas of the Korean peninsula, such as the East Sea, the South Sea and the West Sea itself.