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Hong, Inpyo,Khalid, Alharthi Waleed,Pae, Hyung-Chul,Cha, Jae-Kook,Lee, Jung-Seok,Paik, Jeong-Won,Jung, Ui-Won,Choi, Seong-Ho Korean Academy of Periodontology 2020 Journal of Periodontal & Implant Science Vol.50 No.1
Purpose: To overcome several drawbacks of chemically-crosslinked collagen membranes, modification processes such as ultraviolet (UV) crosslinking and the addition of biphasic calcium phosphate (BCP) to collagen membranes have been introduced. This study evaluated the efficacy and biocompatibility of BCP-supplemented UV-crosslinked collagen membrane for guided bone regeneration (GBR) in a rabbit calvarial model. Methods: Four circular bone defects (diameter, 8 mm) were created in the calvarium of 10 rabbits. Each defect was randomly allocated to one of the following groups: 1) the sham control group (spontaneous healing); 2) the M group (defect coverage with a BCP-supplemented UV-crosslinked collagen membrane and no graft material); 3) the BG (defects filled with BCP particles without membrane coverage); and 4) the BG+M group (defects filled with BCP particles and covered with a BCP-supplemented UV-crosslinked collagen membrane in a conventional GBR procedure). At 2 and 8 weeks, rabbits were sacrificed, and experimental defects were investigated histologically and by micro-computed tomography (micro-CT). Results: In both micro-CT and histometric analyses, the BG and BG+M groups at both 2 and 8 weeks showed significantly higher new bone formation than the control group. On micro-CT, the new bone volume of the BG+M group (48.39±5.47 ㎣) was larger than that of the BG group (38.71±2.24 ㎣, P=0.032) at 8 weeks. Histologically, greater new bone area was observed in the BG+M group than in the BG or M groups. BCP-supplemented UV-crosslinked collagen membrane did not cause an abnormal cellular reaction and was stable until 8 weeks. Conclusions: Enhanced new bone formation in GBR can be achieved by simultaneously using bone graft material and a BCP-supplemented UV-crosslinked collagen membrane, which showed high biocompatibility and resistance to degradation, making it a biocompatible alternative to chemically-crosslinked collagen membranes.
Inpyo Hong,SO Woo,SM Han,SG Kim 한국버섯학회 2017 버섯 Vol.21 No.2
Honey production from approximately 1.7 million colonies owned by around 21 thousand beekeepers was almost 36 thousand M/T in Korea. Pollen has used as a food and medicine from before the Joseon Dynasty period in Korea. Pollen grains such as acorn (Quercus acutissima), actinidia (Actinidia arguta) and green tea (Camellia sinensis) are popular in the markets in Korea. But stiff pollen wall hindered dissolution of polysaccharides and lowered extraction efficiency. In the present study, we measured the antioxidant activity and the total polyphenol content from the pulverized and lyophilized green tea pollen grains inoculated with 6 kinds of fungi to confirm the husk removal effect. The total polyphenol content of green tea pollens was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in green tea pollen germinated with A. mellea. The total polyphenol content of the lyophilized green tea pollens germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH free radical scavenging method showed that the lyophilized green tea pollen grains germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized green tea pollen grains germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of green tea pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from green tea pollen grains inoculated with A. mellea.
리눅스 클러스터 시스템 기반의 고성능 연결 망 벤치마크
홍인표(InPyo Hong) 한국정보과학회 2007 한국정보과학회 학술발표논문집 Vol.34 No.2B
여러 대의 컴퓨팅 시스템을 네트워크로 연결한 클러스터 시스템의 경우, 뛰어난 가격대비 성능 특성 때문에 많은 공학분야의 연구에 널리 활용 되고 있다. 최근에는 뛰어난 확장성과 안정성을 요구하는 기업체 업무에도 널리 활용 되고 있다. 이러한 클러스터 시스템의 성능은 네트워크 시스템의 성능에 크게 좌우되므로 고성능 네트워크 시스템에 대한 연구는 지속적으로 수행되고 있다. 하지만 새로운 네트워크 시스템의 성능이 실제 응용 소프트웨어들의 성능에 어떠한 영향을 주는지 파악하기란 쉽지가 않다. 따라서 여러 개의 응용 소프트웨어 및 계산 소프트웨어에 대한 서비스를 제공해야 하는 기업업무 환경하에서 클러스터 시스템 기반의 새로운 고성능 네트워크 시스템 선택 시 벤치마크는 필수적이다. 본 연구에서는 최근에 출시된 고성능 네트워크 시스템 (Infiniband, Myrinet)들에 대해서 효율적인 노드들간 데이터 통신의 성능을 벤치마크 툴을 통하여 그 결과를 비교 분석하고자 한다.
Isolation of Abscisic Acid from Korean Acacia Honey with Anti- <i>Helicobacter pylori</i> Activity
Kim, SeGun,Hong, InPyo,Woo, SoonOk,Jang, HyeRi,Pak, SokCheon,Han, SangMi Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50
<P><B>Background:</B></P><P><I>Helicobacter pylori</I> (<I>H. pylori</I>) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide.</P><P><B>Objective:</B></P><P>This study is aimed at evaluating the anti-<I>H. pylori</I> activity of Korean acacia honey and isolating the related active components using organic solvents.</P><P><B>Material and Methods:</B></P><P>The crude acacia honey was extracted with <I>n</I>-hexane, dichloromethane, ethyl acetate (EtOAc), and <I>n</I>-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-<I>H. pylori</I> activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against <I>H. pylori</I> was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay.</P><P><B>Results:</B></P><P>Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36–72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against <I>H. pylori</I>.</P><P><B>Conclusion:</B></P><P>Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for <I>H. pylori</I>-induced infections.</P><P><B>SUMMARY</B></P><P><P>The crude acacia honey was extracted with <I>n</I>-hexane, dichloromethane, EtOAc, and <I>n</I>-butanol</P><P>The EtOAc extract yielded eight fractions and four subfractions were subsequently obtained chromatographically</P><P>Abscisic acid was isolated from one subfraction</P><P>All the solvent extracts and fractions showed antibacterial activity against <I>H. pylori</I></P><P>Abscisic acid exhibited antibacterial activity against <I>H. pylori</I>.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error.</P>