Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

외국인근로자 정책 및 법제개선에 관한 연구 - 독일의 외국인근로자 법제를 중심으로 -

백종인 ( Jong In Baek ),설재영 ( Jae Young Seol ) 고려대학교 법학연구원 2007 고려법학 Vol.0 No.49

Korea is going to be faced with the aging society within years. That means our society will lack of economically active population because of an increase in the number of older people soon. The age composition of Korea is expected to move from one of mainly young people who can work to one in which the number of older people will outnumber the young. it is also expected that by 2050 the number of aged people will be doubled compared with the young. We can not help overcoming this problem through foreign workers. So Korea took "the foreign industrial trainee system" to accept the international movement of human capital and then has operated this system with changing into working permit system since August 2004. Germany had experienced the need of work force with the rapid economical renaissance and introduced the industrial trainee system to solve this problem in 1950`s. However, with the principle of circulated introduction of foreign workers in 1964, this system had no meaning of great importance anymore. In 1970`s, being abolished this system because of the increased cost for introducing new work forces, foreign workers could hold the permanent residentship legally possible for inviting their family members who lived their mother countries. Despite an action suspending the recruitment for unskilled foreign work force was operated in 1973, since then, the permanent residence system had been criticized. Through a series of policies for introducing the foreign workers, Germany became an immigrant country. Korea enacted the Act for Employment Foreign Workforce and operated the employment permit system in August 2004. However, this policy has many problems such as legal loopholes, lack of rationality of the act and mass-production of illegal sojourners. Therefore, this paper comparatively examines German statutes relating to the foreign workers and our employment permit system and seeks legal and political problems of our systems. And then, it suggests some practical ways to reform these problems.

Validation of Sea Surface Temperature (SST) from Satellite Passive Microwave Sensor (GPM/GMI) and Causes of SST Errors in the Northwest Pacific

Kim, Hee-Young,Park, Kyung-Ae,Chung, Sung-Rae,Baek, Seon-Kyun,Lee, Byung-Il,Shin, In-Chul,Chung, Chu-Yong,Kim, Jae-Gwan,Jung, Won-Chan The Korean Society of Remote Sensing 2018 大韓遠隔探査學會誌 Vol.34 No.1

Passive microwave sea surface temperatures (SST) were validated in the Northwest Pacific using a total of 102,294 collocated matchup data between Global Precipitation Measurement (GPM) / GPM Microwave Sensor(GMI) data and oceanic in-situ temperature measurements from March 2014 to December 2016. A root-mean-square (RMS) error and a bias error of the GMI SST measurements were evaluated to $0.93^{\circ}C$ and $0.05^{\circ}C$, respectively. The SST differences between GMI and in-situ measurements were caused by various factors such as wind speed, columnar atmospheric water vapor, land contamination near coastline or islands. The GMI SSTs were found to be higher than the in-situ temperature measurements at low wind speed (<6 m/s) during the daytime. As the wind speed increased at night, SST errors showed positive bias. In addition, other factors, coming from atmospheric water vapor, sensitivity degradation at a low temperature range, and land contamination, also contributed to the errors. One of remarkable characteristics of the errors was their latitudinal dependence with large errors at high latitudes above $30^{\circ}N$. Seasonal characteristics revealed that the errors were most frequently observed in winter with a significant positive deviation. This implies that SST errors tend to be large under conditions of high wind speeds and low SSTs. Understanding of microwave SST errors in this study is anticipated to compensate less temporal capability of Infrared SSTs and to contribute to increase a satellite observation rate with time, especially in SST composite process.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

Hardware-in-the-loop 시뮬레이션을 이용한 차량의 종방향 제어기 설계 및 검증

송봉섭(Bongsob Song),백운혁(Woon-Hyuk Baek),신영호(Young-Ho Shin),송호인(Ho-In Song) 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

In this paper, design and validation procedures for a longitudinal controller via hardware-in-the-loop simulation are presented. As new technologies related with ECU and in-vehicle network have been developed, the corresponding longitudinal controller for velocity control of the vehicle needs to be modified and validated experimentally. Since much development cost and effort during the design and validation process are in general required, a relatively quick and efficient design and validation method via hardware-in-the-loop simulation (HILS) is proposed in the paper. Finally speed controller based on Dynamic Surface Control is designed and its performance results are validated via HILS.

허혈 조건부여가 심근기능의 회복에 미치는 영향 : 적출 심장모델을 이용한 실험연구 Isolated heart experimental study

백광제,김준식,한승백,전영진,이인성 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Brief episode of coronary artery occlusion(i.e.,ischemic preconditioning) makes the heart more resistant to injury from a subsequent ischemic insult. Although a great deal of effort has been made in studying ischemic preconditioning, the underlying mechanism of ischemic preconditioning and its effect on hypothermic insult has not been elucidated. This study was performed to see whether ischemic preconditioning protects against the depression of cardiac contractility induced by hypothermic cardioplegic arrest/reperfusion. And recently, adenosine was known to have some correlation with the mechanism of preconditioning. If so, does this effect remain after the blockade of adenosine receptor by 8-phenyl theophyline? Method: Twenty-four Sprague-Dawley rat weighed 250-350g were used and divided into three groups. Rat hearts were removed rapidly, and each isolated heart paced with a rate of 180/min was perfused by modified Krebs-Hensleit buffer(KHB) solution on a Langendorff apparatus for an hour. After obtaining baseline data including left ventricular pressure(LVP), dp/dt, and coronary flow, cardiac arrest was induced by perfusion of 0℃ crystalloid cardioplegic(St Thomas) solution. After that, all hearts were stored in the same St Thomas solution at same temperature for 2 hours. In group Ⅰ(control group), the heart was reperfused by KHB solution. In group Ⅱ(preconditioning group), the heart was subjected to two 2-minute episode of global ischemia followed by 5 minute reperfusion with KHB solution(preconditioning) before cardiac arrest. In group Ⅲ(phenyl theophylline group), the heart was subjected to preconditioning procedure and 8-phenyl theophylline at 10μM in concentration was added to KHB solution at time of reperfusion. Observing parameter was obtained in each group at 10, 20, 40 and 60 minutes after starting reperfusion and compared statistically by use of one way ANOVA test(STASTICA, release 4.5). p-value less than 0.05 was considered significant. Results: Although depressed LVP, dp/dt, and coronary flow were seen in all groups during the reperfusion period, the preconditioned group showed more effective recovery of LVP than that of the control group, especially at 10, 20 and 40 minutes(p<.05). We failed to demonstrate the difference between the phenyl theophylline group and the control group(p=NS). Conclusion: These results suggest that ischemic preconditioning has protective effect on recovery state of hypothemic cardioplegic arrest/reperfusion. Its protective effect was limited during early reperfusion stage and was blocked by adenosine blocker.

홍국을 이용한 발효주의 품질특성

백승희;이지수;배인영 한양대학교 2008 韓國 生活 科學 硏究 Vol.28 No.1

Six kinds of mash were prepared from the mixture of white rice and red fermented rice. Changes in pH, acidity, brix, alcohol, reducing sugar, and color were analyzed during the fermentation period. Quality of the final product was also determined through the sensory evaluation. During the fermentation period, pH and reducing sugar decreased, whereas acidity, brix, and alcohol increased in six mashes. Brix and alcohol of the final mashes decreased significantly with increasing levels of red fermented rice. As the adding ratio of red fermented rice increased, lightness decreased but redness and yellowness increased. Significant differences in sensory evaluation except flavor were observed for all samples (p<0.05), and the best overall acceptability was obtained from the mash prepared with 80 to 100% red fermented rice.

이동물체의 기하학적 특성에 기인한 움직임 정보 추출

박인전,오미숙,백경주,정영철,김신규,박종안 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1

In this paper, we have proposed an effective moving objects tracking method based on the motion information in the video sequence. First, we extract the moving objects using the processes of morphological dilation and differential image in the video sequence and then we extract the motion information utilizing the location and orientation of the moving objects in the subsequent images. The motion information is extracted using various statistical measurements, extracting the high-level features from the regions of pixels in the images. The measurements, such a s area, length, perimeter, orientation, elongation, compactness, moments of inertia, are used as the statistical and geometrical descriptors. The principle axes of an object on this features gives the local direction of the object, while the center of gravity gives the center position of the object. The angle between two principal axes of the moving objects in the consecutive images gives the geometrical direction of the moving object, while the displacement of the center of gravity, gives the distance covered by the moving objects. So we have extracted the global motion information using the principal axes and center of gravity. The simulation results on the test images are very good, where either the motion is very slow, or the frame rate is very high.

RUS법에 의한 Micro-Crack을 가진 Ferrule의 주파수 특성 평가

백경윤,이길성,양순호,김성훈,조영재,민한기,양인영 朝鮮大學校 機械技術硏究所 2003 機械技術硏究 Vol.6 No.2

광섬유 정렬부품으로 사용되는 페롤은 극히 높은 정밀도를 요하는 제품으로서, 높은 치수 정밀도와 결함이 포함되지 않는 등 높은 신뢰성이 요구되는 제품이다. 이러한 페롤의 형상결함평가에 종래의 광학현미경 평가법과는 달리. 본 논문에서는 공명초음파법(ResonantUltrasound Spectroscopy)을 이용하고자 한다. 공명초음파법이란 결함이 없는 시험편을 기계적으로 진동시켰을 때의 공진 주파수와 시험편 내에 결함을 가지고 있을 때의 공명 주파수 양상을 비교 분석하여 결함유무를 검출해내는 방법이다. 본 연구에서는 양품과 크랙을 가진 시험편을 현장에서 직접 사용되고 있는 광학현미경에 의해 분류를 한 다음, 더욱 정확한 측정을 위해 SEM에 의해 다시 분류하였다. 각각에 대해 공진 주파수를 측정하였고, 이 데이터의 검증을 위해 FEM해석을 수행하였다. 실험으로부터 얻는 양품의 공진 주파수와 FEM해석을 통해 얻었던 양품의 공진 주파수의 오차는 1∼2%정도의 범위를 벗어나지 않음을 알 수 있었다. 또한 크랙에 대한 공진 주파수를 측정하여서, 양품의 공진 주파수와 비교를 행하였고, 이 데이터를 가지고 모드별로 히스토그램을 그려서, 그것으로부터 현장에서 실제로 이용 가능한 모드를 존재함을 확인하였다. The Ferrule which is used as the product optical fiber very precisely, is required high to set the optical axes of an dimensional precision, lack of defect and high expectation. Up to now the optical instrument has been used for the defect and shape inspection of the Ferrule, but in this paper we examined the detectable defect and expectation by using the Resonant Ultrasound Spectroscopy (RUS). The RUS is the measurement' which is to excite specimen and to inspect the difference of natural frequency pattern between acceptable specimen and specimen has some defects. In this paper, we classified specimens into three groups by using She optical instrument; acceptable specimen A-type, 3-type and specimen A-type which has crack. And we reclassified by Scanning Microscope to carry out the more exact measurement. We surveyed the resonant frequency of every specimen, and carried out the simulation from the explicit finite elements code, NasEran t o verify the experiment data. The average error between the resonant frequency of acceptable specimens which is obtained from experiment and that from FEM analysis is in the range of 1-2 percentage. Also, we measured the resonant frequency of specimen which has crack, and compared that of acceptable specimen. We drew histogram by using these data, and confirmed the available mode in the plant.

The Ethanol Extract from Artemisia princeps Pampanini Induces p53-Mediated G1 Phase Arrest in A172 Human Neuroblastoma Cells

Eun Young Park,Kyung-Won Lee,이헌우,Young-Wuk Cho,Nam-In Baek,Hae-Gon Chung,정태숙,최명숙,Kyung-Tae Lee 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.2

In the present study, the antiproliferative effects of the ethanol extract of Artemisia princepsPampanini (EAPP)and the mechanism involved were investigated. Of the various cancer cells examined, human neuroblastoma A172 cells weremost sensitive to EAPP, and their proliferation was dose- and time-dependently inhibited by EAPP. DNA flow cytometryanalysis indicated that EAPP notably induced the G1 phase arrest in A172 cells. Of the G1 phase cycle-related proteins ex-amined, the expressions of cyclin-dependent kinase (CDK) 2, CDK4, and CDK6 and of cyclin D1, D2, and D3 were found tobe markedly reduced by EAPP, whereas cyclin E was unaffected. Moreover, the protein and mRNA levels of the CDK in-hibitors p16INK4a, p21CIP1/WAF1, and p27KIP1 were increased, and the activities of CDK2, CDK4, and CDK6 were reduced.Furthermore, the expressions of E2F-1 and of phosphorylated pRb were also decreased, and the protein levels of p53 andpp53 (Ser15) were increased. Up-regulation of p21CIP1/WAF1was found to be mediated by a p53-dependent pathway in EAPP-induced G1-arrested A172 cells. When these data are taken together, the EAPP was found to potently inhibit the proliferationof human neuroblastoma A172 cells via G1 phase cell cycle arrest.