내시경으로 진단된 소화성 궤양의 임상적 고찰

김영건,육은주,김성걸,임의혁,성자원,김병호,허승식,이기천,정현용,이헌영 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

A clinical analysis was performed of 3055 pateints with peptic ulcer who were diagnosed with gastroduodenoscopy, in Hospital of Chungnam National University College of Medicine from July 1988 to May 1993. 1) During the period 1988-1993, The proportion of gastric ulcer among peptic ulcer decreased from 47.8% to 42.8%, but that of duodenal ulcer increased from 36.8% to 39.6%. 2) Of the 3055 cases, the number of patients with esophageal ulcer was 98(3.2%), with gastric ulcer 1407(41.6%), with. duodenal ulcer 1104(36.1%), with channel ulcer 149(4.9%), and with combind ulcer 297(9.7%). 3) We observed a peak incidence of peptic ulcer in the 50-69age group, gastric ulcer in the 5069age, duodenal ulcer in the 40-59age group. The ratio of male to female was 3.6 : 1 in peptic ulcer, 4.4 : 1 in gastric ulcer, 3.0 : 1 in duodenal ulcer. 4) The common site was angle in gastric ulcer, and bulb in duodenal ulcer. 5) The active stage was 39.4% of gastric ulcer, and 37.4% of duodenal ulcer, the healing stage was 34.2% of gastric ulcer, and 17.9% of duodenal ulcer, and the scar stage of gastric ulcer was 26.4%, and duodenal ulcer was 44.7%. 6) The size of ulcer was less than 1Cm in 66.7% of gastric ulcer, and in 75.5% of duodenal ulcer, the size of ulcer was greater than 2Cm in 13.2% of gastric ulcer, and in 6.5% of duodenal ulcer. 7) The frequency of multiple ulcer was 29.9% in gastric ulcer, and 16.5% in duodenal ulcer.

Evaluation of General Toxicity and Genotoxicity of the Silkworm Extract Powder

Hyun-Suk Heo,Jae-Hun Choi,Jung-Ja Oh,Woo-Joo Lee,Seong-Sook Kim,Do-Hoon Lee,Hyun-Kul Lee,Si-Whan Song,Kap-Ho Kim,Yang-Kyu Choi,Kang-Sun Ryu,Boo-Hyon Kang 한국독성학회 2013 Toxicological Research Vol.29 No.4

The silkworm extract powder contain 1-deoxynojirimycin (DNJ), a potent α-glycosidase inhibitor, has therapeutic potency against diabetes mellitus. Therefore, natural products containing DNJ from mulberry leaves and silkworm are consumed as health functional food. The present study was performed to evaluate the safety of the silkworm extract powder, a health food which containing the DNJ. The repeated toxicity studies and gentic toxicity studies of the silkworm extract powder were performed to obtain the data for new functional food approval in MFDS. The safety was evaluated by a single-dose oral toxicity study and a 90 day repeated-dose oral toxicity study in Sprague-Dawley rats. The silkworm extract powder was also evaluated for its mutagenic potential in a battery of genetic toxicity test: in vitro bacterial reverse mutation assay, in vitro chromosomal aberration test, and in vivo mouse bone marrow micronucleus assay. The results of the genetic toxicology assays were negative in all of the assays. The approximate lethal dose in single oral dose toxicity study was considered to be higher than 5000 mg/kg in rats. In the 90 day study, the dose levels were wet at 0, 500, 1000, 2000 mg/kg/day, and 10 animals/sex/dose were treated with oral gavage. The parameters that were monitored were clinical signs, body weights, food and water consumptions, ophthalmic examination, urinalysis, hematology, serum biochemistry, necropsy findings, organ weights, and histopathological examination. No adverse effects were observed after the 90 day administration of the silkworm extract powder. The No-Observed-Adverse-Effect-Level (NOAEL) of silkworm extract powder in the 90 day study was 2000 mg/kg/day in both sexes, and no target organ was identified.

감잎차 추출액의 Sister Chromatid Exchange(SCE) 방법에 따른 항돌연변이 효과

송현순(Hyun-Soon Song),이현걸(Hyun-Kul Lee),장해동(Hae-Dong Jang),김종익(Jong-Ik Kim),박옥진(Ock-Jin Park),이미숙(Mee-Sook Lee),강명희(Myung-Hee Kang) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.2

돌연변이 유발 물질인 mitomycin C(MMC)를 처리하여 배양한 Chinese hamster ovary(CHO) cell에 대한 감잎차 추출액의 항돌연변이 효과를 자매 염색 분체 교환(sister chromatid exchange, SCE) 시험법을 사용하여 측정하여 보았다. 감잎차 추출액 자체는 CHO 세포의 SCE 빈도수를 변화시키지 않았으며, 세포의 분열 주기중 S phase에 S9 mixture없이 감잎차 추출액이 처리되었을 경우 MMC로 유도된 SCE 빈도수를 감소시키지 않았다. 그러나 S9 mixture 존재하에 G₁ phase에서 MMC 처리 후 감잎차를 처리하는 후처리 방식으로 감잎차 추출액을 처리하였을 때, 저농도(≤40㎍/ml)에서 MMC로 인해 유발된 SCE 빈도수가 낮아지는 것을 볼 수 있었다. 이에 비해 고농도 (>40㎍/ml)에서는 SCE 빈도수의 감소 효과가 없었다.<br/> 본 연구 결과, MMC 처리된 CHO 세포에 대한 감잎차 추출액의 항돌연변이 효과를 볼 수 있었고, 이 효과는 S9 mixture 존재하에서 저농도의 감잎차 추출액이 G₁ phase에 처리되었을 때 나타났다. 감잎차 추출액의 이러한 항돌연변이 효과의 기전은 감잎차 추출액의 대사산물이 MMC 처리된 CHO 세포에 대한 DNA-excision repair activity를 촉진시키기 때문인 것으로 생각된다. The antimutagenic effects of persimmon leaf tea extracts(PLTE) on mutagen-induced sister chromatid exchanges(SCEs) were studied. These PLTE did not affect spontaneous SCEs in cultured Chinese hamster cells. The frequency of SCEs induced by mitomycin C(MMC) was not affected by the simultaneous treatment with PLTE without S9 mixture in the S phase of the cell cycle. However, when cells were posttreated with PLTE in the presence of metabolic enzymes of rat liver(S9 mix), the antimutagenic effects on the induction of SCEs by mutagen were observed. MMC-induced SCEs were suppressed by the posttreatment with PLTE at low concentrations(≤40㎍/ml) with S9 mix. In contrast, at a high concentration(>40㎍/ml) of PLTE with S9 mix, MMC-induced SCEs were not affected. The antimutagenic effects of PLTE were shown to occur in the G₁ phase of the cell cycle. The results suggested that PLTE could promote DNA-excision repair activity and resulted in an antimutagenic effect.

Cofactor-free Light-driven Whole-cell Cytochrome P450 Catalysis

Jong Hyun PARK,Sahng Ha LEE,Gun Su CHA,Da Som CHOI,Dong Heon NAM,Jae Hyung LEE,Jung-Kul LEE,Chul-Ho YUN,Ki Jun JEONG,Chan Beum PARK 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.4

연구논문 : 유전독성 대체시험법(in vitro소핵시험)의 국내검증시험연구

이현걸 ( Hyun Kul Lee ),김종극 ( Jong Geuk Kim ),이우주 ( Woo Joo Lee ),맹은호 ( Eun Ho Maeng ),이종윤 ( Jong Yoon Lee ),정영신 ( Young Shin Chung ),김창환 ( Chang Hwan Kim ),김윤순 ( Yun Soon Kim ),장미해 ( Mi Hae Jang ),이석종 ( 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1

This validation study was performed to introduce and set up the in vitro micronucleus test, as an alternative to the existing chromosome aberration test, in the test facilities in Korea, and to establish the competence of each facility to perform the in vitro micronucleus test independently. The 10 coded substances, including 6 positive and 4 negative in genotoxicity assay, were tested. Six facilities were divided into two subgroups, A and B. Three coded positive compounds and two negative compounds were sent to each test facility. The compounds were tested according to the facility`s GLP system. In group A, positive results were obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. In group B, positive results were also obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. For the Urethane, an in vitro equivocal compound tested by group B, negative results were obtained. These results suggested that the compound may be effectively activated only in in vivo environment. The results show that the six test facilities are capable of performing the in vitro micronucleus test independently and producing consistent results.

Characterization of Low-Crystalline Calcium Phosphate Thin Films Formed on Silica Glass Surface

Woo-Kul Lee,Man Jin Mo,Hyun Young Shin,Il Hyun Jung,Jea Seung Ko,Joseph McGuire 한국공업화학회 2005 Journal of Industrial and Engineering Chemistry Vol.11 No.2

Evaluation of 13-week repeated oral dose toxicity of <i>Areca catechu</i> in F344/N rats

Kim, Hyun-Ji,Ko, Je-Won,Cha, Seung-Beom,Heo, Hyun-Suk,Seo, Jong-Hun,Cha, Mi-Jin,Bae, Jin-Sook,Lee, Hyun-Kul,Song, Si-Whan,Kim, Jong-Choon Elsevier 2018 Food and chemical toxicology Vol.114 No.-

<P><B>Abstract</B></P> <P>This study investigated the potential toxicity of the Areca catechu water extract after 13-week repeated oral administration at 0, 166.7, 500, and 1500 mg/kg/day in rats. During the study period, clinical signs, mortality, body weight, food consumption, water consumption, urinalysis, estrous cycle, sperm count and motility, ophthalmoscopy, hematology, serum biochemistry, gross pathology, organ weights, and histopathology were examined. At 1500 mg/kg/day, both sexes exhibited an increase in the incidence of abnormal clinical signs, which included, decreased body weight gain and food consumption, and increased urine bilirubin, ketone bodies, specific gravity, and protein and kidney weight. An increase in liver weight and estrous cycle alterations was observed in females. Serum biochemical and histopathological investigations revealed an increase in the levels of serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase, and the incidence of hepatic necrosis in females. At 500 mg/kg/day, an increase in the incidence of abnormal clinical signs including diarrhea and soiled perineal region, was observed in both sexes. No treatmentrelated effects were observed at 166.7 mg/kg/day. Under the present experimental conditions, the target organs were determined to be the liver, kidney, and female reproductive system in rats. The no-observedeffect level was considered to be 166.7 mg/kg/day in rats.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Subchronic toxicity of <I>Areca catechu</I> water extract was investigated in rats. </LI> <LI> The target organs of the extract were determined to be the liver, kidney, and female reproductive system. </LI> <LI> The no-observed-effect level of the extract was considered to be 166.7 mg/kg/day in rats. </LI> </UL> </P>

Evaluation of General Toxicity and Genotoxicity of the Silkworm Extract Powder

Heo, Hyun-Suk,Choi, Jae-Hun,Oh, Jung-Ja,Lee, Woo-Joo,Kim, Seong-Sook,Lee, Do-Hoon,Lee, Hyun-Kul,Song, Si-Whan,Kim, Kap-Ho,Choi, Yang-Kyu,Ryu, Kang-Sun,Kang, Boo-Hyon Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.4

The silkworm extract powder contain 1-deoxynojirimycin (DNJ), a potent ${\alpha}$-glycosidase inhibitor, has therapeutic potency against diabetes mellitus. Therefore, natural products containing DNJ from mulberry leaves and silkworm are consumed as health functional food. The present study was performed to evaluate the safety of the silkworm extract powder, a health food which containing the DNJ. The repeated toxicity studies and gentic toxicity studies of the silkworm extract powder were performed to obtain the data for new functional food approval in MFDS. The safety was evaluated by a single-dose oral toxicity study and a 90 day repeated-dose oral toxicity study in Sprague-Dawley rats. The silkworm extract powder was also evaluated for its mutagenic potential in a battery of genetic toxicity test: in vitro bacterial reverse mutation assay, in vitro chromosomal aberration test, and in vivo mouse bone marrow micronucleus assay. The results of the genetic toxicology assays were negative in all of the assays. The approximate lethal dose in single oral dose toxicity study was considered to be higher than 5000 mg/kg in rats. In the 90 day study, the dose levels were wet at 0, 500, 1000, 2000 mg/kg/day, and 10 animals/sex/dose were treated with oral gavage. The parameters that were monitored were clinical signs, body weights, food and water consumptions, ophthalmic examination, urinalysis, hematology, serum biochemistry, necropsy findings, organ weights, and histopathological examination. No adverse effects were observed after the 90 day administration of the silkworm extract powder. The No-Observed-Adverse-Effect-Level (NOAEL) of silkworm extract powder in the 90 day study was 2000 mg/kg/day in both sexes, and no target organ was identified.

유전독성 대체시험법(in vitro소핵시험)의 국내검증시험연구

이현걸 ( Hyun Kul Lee ),김종극 ( Jong Geuk Kim ),이우주 ( Woo Joo Lee ),맹은호 ( Eun Ho Maeng ),이종윤 ( Jong Yoon Lee ),정영신 ( Young Shin Chung ),김창환 ( Chang Hwan Kim ),김윤순 ( Yun Soon Kim ),장미해 ( Mi Hae Jang ),이석종 ( 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1

This validation study was performed to introduce and set up the in vitro micronucleus test, as an alternative to the existing chromosome aberration test, in the test facilities in Korea, and to establish the competence of each facility to perform the in vitro micronucleus test independently. The 10 coded substances, including 6 positive and 4 negative in genotoxicity assay, were tested. Six facilities were divided into two subgroups, A and B. Three coded positive compounds and two negative compounds were sent to each test facility. The compounds were tested according to the facility`s GLP system. In group A, positive results were obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. In group B, positive results were also obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. For the Urethane, an in vitro equivocal compound tested by group B, negative results were obtained. These results suggested that the compound may be effectively activated only in in vivo environment. The results show that the six test facilities are capable of performing the in vitro micronucleus test independently and producing consistent results.

The Korean Society of Gastroenterology& SLDDS 2028 : Slide Session ; K-BP-18 : Pancreatobiliary ; Gallstone Dissolution Effi cacy According to Stone Density on CT Scan

( Jong Jin Hyun ),( Jae Min Lee ),( Seung Young Kim ),( Sang Jun Suh ),( Sung Woo Jung ),( Young Kul Jung ),( Ja Seol Koo ),( Hyung Joon Yim ),( Hong Sik Lee ),( Sang Woo Lee ),( Chang Duck Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Currently available medications to dissolve gallbladder stones are ursodeoxycholic acid(UDCA) or a combination of chenodeoxycholic acid(CDCA) and UDCA. In the previous studies, dissolution effi cacy had been compared after excluding patients with stones evident on plain abdominal X-ray but CT scan was not routinely performed to evaluate the presence of calcifi cation. This study was conducted to compare the dissolution effi cacy of UDCA alone or a combination of CDCA and UCDA(CNU) according to stone density on CT scan. Methods: Among a total of 393 gallbladder stone patients who presented to the outpatient department of Korea University Ansan Hospital from December 2010 to March 2014, 124 patients underwent dissolution therapy with either CNU(n=61) or UDCA(n=63). Of these patients, 53 were excluded because of follow-up loss (n=37) or symptom development necessitating cholecystectomy(n=6). In the end, 71 patients (CNU group = 42, UDCA group = 29) were included for analysis. Dissolution was considered effective if the largest stone size diameter showed decrease of >50% or completely dissolved. Stone density on CT scan was divided into four groups: hypodense,isodense, hyperdense, and calcifi ed. Results: The baseline age (49.40±14.85 years vs. 53.59±19.90 years), treatment duration (183.07±16.02 days vs. 180.48±16.10 days), and pre-treatment stone size (8.74±4.25mm vs. 9.20±4.50mm) were not different between the CNU group and UDCA group. Effective dissolution was observed in 26.2% (11/42) and 48.3% (14/29) of patients after CNU and UDCA treatment, respectively (p=0.055). When only those with stones that were hypodense or isodense on CT scan were analyzed, the effective dissolution rate rose to 57.1% (8/14) and 75% (9/12) with CNU and UDCA treatment, respectively (p=0.429). Conclusions: Patients with gallbladder stones that were hypodense or isodense showed much better dissolution effi cacy. Therefore, CT scan should be performed prior to medication therapy if stone dissolution is intended.