바이칼린 함유 생약의 제제화 및 생체 이용률 (제 2보) : 황금 및 황련 공침물의 장내 흡수 및 항균 효과 Gastro-Intestinal Absorption and Antibacterial Effect of Coprecipitated Product of Scutellariae Radix and Coptidis Rhizoma

양재헌,김동수,류희두,이남희 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Precipitation was formed during the preparation of decoction from mixure of Scutellariae Radix and Coptidis Rhizoma or Phellodendri Cortex according to the prescription of Hwang-ryean-hae-dog-tang. Baicalin and berberine, the active ingredients of the two herbal medicine were identified in coprecipitated product. Pills were prepared using the coprecipitated product and various binders. The dissolution rate of baicalin and berberine from pills was increased in at pH1.2 when acacia or tragacanth was used. The absorption rate of baicalin from the coprecipitated product was raster than the from Scutellaria extract, but the absorption of berberine from CPP was slower in stomach, duodenum and jejunum of rats compared with Coptis expract. The time equired for the maximum serum concentration (Cmax) of baicalin and berberine from CPP in mice were 150 and 200 min after oral administration. respectively. The maximum serum concentration of baicalin from CPP in mice was higher than Scutellaria extract, but the concentration of berberine was lower compared with Coptis extract. The minimum inhibitory concentration of CPP was below 50 ug/ml against gram positive bacteria, and was higher than that against gram negative bacteria. The antibacterial activity of CPP was lower than the of berberine.but was more potent than Scutellaria extract. It was found that the ingibition rates of growth by CPP against S. cpidermidis, K. pneumoniae, B cereus and S. aureus were 60.1, 5.11, 45.4, and 39.9%. respectively.

대퇴골 간부의 장사상 또는 나선상 골절 치료에 있어 역학적 압박 금속판과 나사고정의 비교

이성태,김동헌,김선남 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

Fractures of the femoral shaft are among the common fractures enconutered in orthpedic practice and resulted from high energy. They bring many associated injury and have severe soft tissue injuries. Today, the interlocking intramedullary nail is regarded as a treatment of choice for fractures of the femoral shaft. But as to long oblique and spiral fractures of femoral shaft, dynamic compression plate and screw fixation are much more used. The department of Orthopedic Surgery, Seoul hospital, Kon-Kuk University Medical center reports about two treatments and the result by comparing between 24 cases who were treated by dynamic compression plates and 20 cases who were treated by screw fixation for long oblique and spiral fractures for femoral shaft from January 1992 to December 1996. The results were as follows: 1. Bony union was finished at average 38.3 weeks, at average 39.5 weeks in cases used dynamic compression plates, at average 37.8 weeks in cases used screw fixation. 2. Complications were 6 cases of joint stiffness of ipsilateral knee joint, 4 cases of nonunion, 2 cases of delayed union, 2 cases of angular deformity, and 1 case of limb shortening. But there was no significant difference between two treatments. 3. Bony union of long oblique and spiral fractures of the femoral shaft that treated by dynamic compression plates and screw fixation was done by so-called primary bone healing. 4. In the treatment of long oblique and spiral fractures of the femoral shaft, screw fixation was no difference in complication but better in bony union than using dynamic compression plate. So it seems to be a good treatment method for long oblique and spiral fractures of the femoral shaft.

자궁경부에 발생한 배아성 횡문근육종 2예

박정열,조준식,김대연,이동헌,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.3

횡문근육종은 소아와 청소년기에 가장 흔한 연부조직 육종이다. 발생 부위는 일반적으로 두부 및 경부, 그 다음으로 비뇨생식계이다 조직학적으로 배아성 횡문근육종, 국화상 육종, 폐포성 횡문근육종, 다형성 횡문근육종으로 나누어진다. 횡문근육종의 약 20%가 비뇨생식기에서 발생하고, 50% 이상이 배아성 횡문근육종이다. 여성 생식기의 배아성 횡문근육종은 드문 악성 종양으로,주로 유소아의 질에서 발생하며, 자궁경부에서 발생하는 횡문근육종은 청소년기에 흔하고, 질에서 기원하는 횡문근육종이 자궁경부에 기원하는 경우보다 5배 정도 많다. 폐경기여성에서는 자궁체부에서 가장 흔히 발생한다. 비뇨생식기의 횡문근육종의 치료는 과거에는 골반장기 적출술만으로 치료를 시도하였으나. 서서히 다중 항암화학요법, 방사선요법, 근치적 절제술을 포함한 병합요법으로 바뀌어가고 있으며, 이에 따라 생존율이 현저하게 향상되었다. 이에 저자들은 최근 본원 산부인과에서 자궁경부에 발생한 배아성 횡문근육종 2예를 경험하였기에 문헌 고찰과 아울러 보고하는 바이다. Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood and young adult. Genitourinary tract is the econd most common site of rhabdomyosarcoma. Rhabdomyosarcoma can be classified into one of four major types; embryonal, alveolar, pleomorphic and undifferentiated. Embryonal rhabdomyosarcoma of the female genital tract is rare malignant tumor and usually occurs during childhood in the vagina. In rare cases, rhabdomyosarcoma can originate in the uterine cervix, with a peak incidence in the second decade. Recently we have experienced two cases of embryonal rhabdomyosarcoma of the uterine cervix. These cases are presented with a brief review of the literature.

고량강의 항염증 작용에 대한 연구

박히준,이제현,김수영,심범상,구헌종,강전모,최일환,이재동,김남재,이지숙,임사비나 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : The use of herbal therapy is becoming an increasingly attractive approach for the treatment of various inflammatory disorders. The Alpiniae officinari Rhizoma is popular in Aisa as a traditional herbal medicine. Alpiniae officinari Rhizoma is a species of the ginger family(Zingiberacease). Method : This study was performed to investigate the anti-inflammatory effect of Alpiniae officinari Rhizoma extract by the methods of "carrageenan induced paw edema" and "Lipopolysaccharide-induced inflammatory mediators in mouse macrophage RAW 264.7 cells". Result : We suggest that Alpiniae officinari Rhizoma extract decreased paw volume induced by plantar injection of carrageenan. Also Alpiniae officinari Rhizoma extract inhibited nitric oxide, prostaglandin E₂production and induced nitric oxide synthase, cyclooxygenase-2 protein expression in Mouse macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Conclusion : This study shows that Alpiniae officinari Rhizoma extract seems to have anti-inflammatory effect by inhibition of nitric oxide, prostaglandin E_(2) production and nitric oxide synthase, cyclooxygenase-2 protein expression.

Water oxidation-coupled, photoelectrochemical redox biocatalysis toward mimicking natural photosynthesis

Nam, Dong Heon,Ryu, Gyeong Min,Kuk, Su Keun,Choi, Da Som,Son, Eun Jin,Park, Chan Beum Elsevier 2016 Applied Catalysis B Vol.198 No.-

<P><B>Abstract</B></P> <P>Redox enzymes are industrially important for catalyzing highly complex reactions because of their excellent regio- and stereo-selectivity; however, broad application of redox enzymes has been often limited by the requirement of stoichiometric supply of cofactors such as β-nicotinamide adenine dinucleotide (NADH). Here, we report light-driven cofactor regeneration coupled with water oxidation by employing a photoelectrochemical cell platform consisted of a FeOOH/Fe<SUB>2</SUB>O<SUB>3</SUB> photoanode and a black silicon photocathode. The FeOOH layer deposited on Fe<SUB>2</SUB>O<SUB>3</SUB> surface decreased reaction barriers for water oxidation. The black silicon photocathode exhibited high photocurrent response and superior capacity to drive cofactor reduction. The cofactor regeneration yield in the photoelectrochemical cell was almost two-fold higher than that obtained in homogenous system, which demonstrates that photoelectrochemical cell is a promising platform for redox biocatalytic reactions using water as an electron donor.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This work offers a new strategy for redox biocatalysis with water as an electron donor. </LI> <LI> FeOOH layer deposited on Fe<SUB>2</SUB>O<SUB>3</SUB> surface decreased reaction barriers for water oxidation. </LI> <LI> Black silicon exhibited superior capacity to drive NAD<SUP>+</SUP> reduction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Solar Water Splitting with a Hydrogenase Integrated in Photoelectrochemical Tandem Cells

Nam, Dong Heon,Zhang, Jenny Z.,Andrei, Virgil,Kornienko, Nikolay,Heidary, Nina,Wagner, Andreas,Nakanishi, Kenichi,Sokol, Katarzyna P.,Slater, Barnaby,Zebger, Ingo,Hofmann, Stephan,Fontecilla‐,Ca John Wiley and Sons Inc. 2018 Angewandte Chemie Vol.57 No.33

<P><B>Abstract</B></P><P>Hydrogenases (H<SUB>2</SUB>ases) are benchmark electrocatalysts for H<SUB>2</SUB> production, both in biology and (photo)catalysis in vitro. We report the tailoring of a p‐type Si photocathode for optimal loading and wiring of H<SUB>2</SUB>ase through the introduction of a hierarchical inverse opal (IO) TiO<SUB>2</SUB> interlayer. This proton‐reducing Si|IO‐TiO<SUB>2</SUB>|H<SUB>2</SUB>ase photocathode is capable of driving overall water splitting in combination with a photoanode. We demonstrate unassisted (bias‐free) water splitting by wiring Si|IO‐TiO<SUB>2</SUB>|H<SUB>2</SUB>ase to a modified BiVO<SUB>4</SUB> photoanode in a photoelectrochemical (PEC) cell during several hours of irradiation. Connecting the Si|IO‐TiO<SUB>2</SUB>|H<SUB>2</SUB>ase to a photosystem II (PSII) photoanode provides proof of concept for an engineered Z‐scheme that replaces the non‐complementary, natural light absorber photosystem I with a complementary abiotic silicon photocathode.</P>

CdTe, CdSe, and CdS Nanocrystals for Highly Efficient Regeneration of Nicotinamide Cofactor Under Visible Light

Nam, Dong Heon,Lee, Sahng Ha,Park, Chan Beum WILEY-VCH Verlag 2010 Small Vol.6 No.8

<B>Graphic Abstract</B> <P>CdTe, CdSe, and CdS nanocrystals have a high capability to drive in situ photochemical regeneration of NADH under visible light (λ > 420 nm). In particular, CdTe nanocrystals exhibit a superior turnover frequency and number for NADH regeneration compared to other inorganic photosensitizers. This work shows that quantum dots are excellent candidates as light harvesting components that can photochemically boost enzymatic synthesis reactions critically requiring cofactors. <img src='wiley_img_2010/16136810-2010-6-8-SMLL201000077-content.gif' alt='wiley_img_2010/16136810-2010-6-8-SMLL201000077-content'> </P>

연구노트 : 왕겨초액의 산화억제 활성 비교

남동윤 ( Dong Yoon Nam ),이시림 ( Si Rim Lee ),박철홍 ( Chul Hong Park ),박규식 ( Kyu Sik Park ),남상헌 ( Sang Heon Nam ),허진철 ( Jin Chul Heo ),이상한 ( Sang Han Lee ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2010 한국식품저장유통학회지 Vol.17 No.3

QTL analysis of the qBK1, a major QTL for bakanae disease resistance in rice

Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.

Identification of a major quantitative trait locus for bakanae disease resistance in rice

Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Bakanae disease incidence threat is an increasing trend in the top rice growing countries. Despite it is essential to identify the resistant genes and underlying mechanisms of bakanae disease to develop resistant varieties, there are very limited genetic studies on bakanae disease in rice. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). The proportion of healthy plants of Shingwang and Ilpum after inoculation was confirmed using bakanae disease pathogen, CF283. While inoculated Ilpum showed thin and yellowish-green phenotype which is typical symptom of Bakanae disease, Shingwang showed similar healthy phenotype with control plants. A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The information of qBK1 could be useful for improving rice bakanae disease resistance in marker-assisted breeding.