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      • KCI등재후보

        GIS를 활용한 효율적인 농촌경관관리구역 설정에 관한 연구

        서주환,최현상,변성진,안재홍,양희승 경희대학교 부설 디자인연구원 2002 예술· 디자인학연구 Vol.5 No.1

        Land use and social, cultural and environmental safety, which are adapted to natural environment, based on the traditional agricultural society, are on the verge of collapse because our rural community was developing around economy value. Rural landscape has not been certified compared with urban and natural landscape in importance by the standard of functional aspect, economic value and use demand, so cultural, historical, traditional and agricultural landscape native to rural community are not evaluated exactly. The Purpose of the study is a efficient plan and management for a essential rural landscape. Therefore We Make out a landscape diagram for efficient landscape plan and management and Selection of the Rural Landscape Management Area.

      • MPEG-2 VBR 비디오 데이터의 트래픽 셰이핑

        서덕영,장승기,경문현,박섭형 경희대학교 산학협력기술연구원 1996 산학협력기술연구논문집 Vol.2 No.-

        This paper describes two traffic shaping methods for VBR(variable bit rate) video bit streams encoded in the MPEG-2 syntax. Difficulties in control of VBR video traffic are lessened by traffic shaping. Burstness of single layer MPEG-2 can be reduced by performing intra-refresh in more than one consecutive frames. In two layer encoding of spatial scalability, burstness can be reduced by setting the temporal locations of GOP starting frame of a layVBR 트래픽을 허용한다는 것이다. 모든 AAL er, differently from the other. Queueing analysis shows that these two methods outperform conventional encoding schemes in terms of temporal and semantic QoS requirements.

      • KCI등재후보

        수목장(樹木葬)의 특성에 관한 고찰

        서주환,최현상,정해천,승지현 경희대학교 디자인연구원 2005 예술· 디자인학연구 Vol.8 No.2

        To secure a space for graveyard in a funeral has become one of the new issues in Korea. This caused the problems of graveyard shortage, destruction of national landscape, inroad of territory, and ecological environment. However, In eco-friendly method of funeral, the Woody Plant Funeral is a new form of Funeral to bury the ashes of the deceased under the wood so it participate with eternal life in wood and forest by provision of nature(wood and people return to nature to be born in nature). The purpose of this paper is to explain one of the counterplan which the Woody Plant Funeral solve graveyards problem.

      • 여성근로자들의 자각적 호흡기 질환과 관련요인 분석

        한성현,서승희,박재성 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

        The objectives of this study is to explain the perceived respiratory disease of female workers and to identify the affecting factors on their perceived respiratory disease. By the results of this study will be provided for basic data to develop health promotion program and to prevent the respiratory disease among female workers. The size of samples was 1875 female workers under forty years old. The data were collected method to identify the affecting factors on their perceived respiratory disease was used multiple linear regression analysis. The results were as follows; The average score of perceived respiratory disease by Cornell medical Index(CMI) is 1.92±2.16 and prevalence rate of respiratory disease is 3.6%. The results by multiple regression analysis were as follows: The affecting factors on CMI of respiratory disease were smoking, stress, workplace accident, status of indoor air pollution, respiratory disease in family, age, working period of current job(R^(2)=0.111). Respiratory health problem of female workers related to personal health behavior, job stress, indoor air-pollution of working place. Therefore the most effective health education for charge of health behavior(smoking) should be considered and must be provided the opportunity for health promotion program in workplace. Also the affecting factors on health status were working environments(indoor air pollution). Therefore, the most safety for working environments should be considered for the female workers in workplace.

      • Canine Distemper Virus 검출을 위한 정량 Real-time PCR

        이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

        Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

      • 유아 영어교육의 지도방법과 교재에 관한 국내 연구의 경향 및 내용 분석

        좌승화,천희영,선현아,최미현 부산유아교육학회 2003 유아교육논총 Vol.12 No.-

        본 연구는 유아영어교육의 지도방법 및 교재에 관한 국내 연구의 경향과 내용을 분석하는데 연구의 직접적인 목적을 두었다. 구체적으로는 조기 영어교육 현장에서 실제 이루어지고 있는 지도 방법 및 교재에 관한 연구논문들의 현황과 실태를 총체적으로 조망하고, 그 결과에 근거하여 추후 유아영어교육 분야에서 지도방법 및 교재 개발에 관한 연구의 방향을 설정하는 기초 자료를 제공한다는 데 궁극적인 목적을 두었다. 연구내용 분석 결과, 동화와 이야기 들려주기를 이용한 영어지도방법의 효과를 가장 많이 연구하고 있었으며, 그 다음으로 게임, 노래, 역할극, 자유선택놀이 활동 등에 대한 연구들이 있었다. 교재연구는 유아교육기관에서 가장 많이 사용하고 있는 교재를 언어적 측면과 실용적 측면 등을 비교, 분석하였다. The purpose of this study was to investigate current research issues and research contents about teaching methods and textbooks in the area of early childhood English education. By analyzing current research trends, it was aimed to view the present situation and the actual status of early childhood English education systematically, and to guide the future research in terms of teaching methods and textbooks in early childhood English education based on current early English education status and situation. Results showed that fairly tales and storytelling were the most used teaching methods in current research following by games, English songs, role play, and utilizing indoor free play time. Current research about textbooks used in early childhood English education surveyed the most used English textbooks in kindergartens and analyzing linguistic and practical aspects of the contents of textbooks.

      • ^27Al(p,α pn)핵반응에 의한 무담체 ^22Na 제법 연구

        유국현,서용섭,양승대 東國大學校 1998 東國論叢 Vol.37 No.-

        A method for the production of no-carrier added(NCA) ^22Na was developed via ^27Al(p, ㄷαpn) nuclear reaction with 50.5MeV protons. the half life of ^22Na is 2.6 years and main γ-energy is 1274.5keV and it is used standard source and sodium catabolism study. The cross-section and thick target yield for the reaction was measured in detail in the energy range of 50.5→20.2MeV in order to determine the optimum conditions for the production of ^22Na. the maximum cross-section for the production of ^22Na was 40.8 mbarn at 43.85MeV. the calculated production yield of ^22Na by 50.5MeV protons on aluminium was 60.9μCi/μAh. The seperation of ^22Na was carried out by ion exchange, precipitation and diffusion. It was found that ion exchange column operation using AG50W-X4 resin was the most efficient method among them.

      • KCI등재
      • 위암세포에 의한 종양침윤 림프구의 면역반응 억제기전에 관한 연구

        박정규,송규상,서광선,최정목,배진선,장일성,윤완희,노승무,조은경,백태현 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

        Tumor-infiltrating lymphocytes ('1°ILs) interact most closely with tumor cells and thus are more likely to reflect tumor host interactions accurately. But it is unknown whether such T cells are nonspecific inflammatory cells or a subset of specific host immune responses. In this study, there was no clear correlation between the infiltration of T lymphocytes in stomach cancer and the overexpression of c-ErbB-2 or increasing class I MHC expression on tumor cells. A positive correlation was seen between the presence of TILs in the tumor and tumors with diploidy by flow cytometric DNA analysis. The proliferative responses of Ills stimulated with IL-2, anti-CD3 mAb, or both were examined. When compared to normal mucosal-associated lymphoid tissue lymphocytes, the proliferative response of TILs to high dose IL-2 was minimal. A similarly poor response to anti-CD3 mAb plus IL-2 was also observed. The freshly isolated TILs exhibit reduced ability to proliferate in response to IL-2, anti-CD3 mAb or both. The microenvironment of the tumor suppresses the proliferative capacity of the TILs. The mechanism of this suppression remains unknown. It could be mediated by suppressor cells, by soluble substances within the tumor, or both. To examine this question, supernatants of stomach cancer cells (SNSNU-1) were tested for the presence of immunosuppressive factors. Human peripheral blood T-cells and tumor-draining lymph node lymphocytes (TDLNL) were incubated for 3 days with SNSNU-1 and then assessed for proliferative responses to PMA, anti-CD28 mAb, or both and for the inducibility to express IFN- r or IL-4 mRNA to PMA. Peripheral blood T-cells pretreated with SNSNU-1 were unable to proliferate in response to PMA, anti-CD28 mAb or both. SNSNU-1 also produces inhibitory activities of TDLNL proliferative response to PMA or anti-CD28 mAb and PMA (49%, 52%, respectively). In contrast, culture supernatants obtained from HEp-2, K562 or Daudi showed normal proliferative responsiveness of peripheral blood T-cells and TDLNL by PMA, anti-CD28 mAb or both.

      • D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

        곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

        To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

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