서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

조기탈형을 위한 라텍스 개질 초속경 콘크리트의 개발 및 강도발현 특성

이봉학,홍창우,이주형,정원경 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

현재 스틸렌-부타디엔 라텍스(styrene-butadiene latex)를 혼입한 콘크리트는 광범위하게 사용되고 있다. 교면포장용으로 라텍스 개질 콘크리트(LMC)의 사용은 일반 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트는 장시간 양생을 요하는 문제로 인해 긴급을 요하는 곳에는 적용할 수 없다. 따라서 본 연구에서는 초속경시멘트를 사용한 라텍스 개질 콘크리트를 개발하고, 이에 따른 작업성 및 강도발현특성을 분석하는데 그 목적이 있다. 실험결과, 지연제와 소포제를 사용한 라텍스 개질 초속경 콘크리트(RSLMC)의 강도발현을 확인할 수 있었으며, 제안된 최적배합은 초속경성의 강도발현으로 인해 현장에서 긴급보수에 적용이 가능함을 알 수 있었다. 따라서 라텍스 개질 초속경 콘크리트는 교면포장, 포장체, 빌딩등의 보수에 적용이 가능할 것으로 판단된다. Concrete containing styrene-butadiene latex is widely used, nowadays, as a protective system for bridge. The usage of latex modified concrete (LMC) as an overlay system is not all that different from any quality concrete, but the improved properties impart to the concrete by the latex. However, this may not be used for repair works because the bridge lane could not be closed for several weeks for curing. Therefore, this study focused on the development of rapid-setting cement latex modified concrete (RSLMC), and on the investigation of workability and strength development. As a result, this study clearly validated the strength development of RSLMC by introducing a retarder and an antifoam agent. The proposed optimized concrete mixtures might be satisfactorily applied for the repair works in fields, because of its rapid strength development, respectively. Therefore, RSLMC could be used in many repair or overlay works such as bridge deck, pavement or building.

콩 펩타이드의 항암 활성

이형주 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-

An anticancer peptide from soy protein was purified and isolated. Defatted soy protein was hydrolyzed with thermoase and its hydrophobic peptides were extracted with ethanol. The peptide extract was fractionated by XAD-2 adsorption, gel filtration chromatography, and different C18 HPLCs. Anticancer activity of each fraction was assayed by measuring in vitro cytotoxicity on P388D1, a mouse monocyte macrophage cell line. IC50 value of a peptide fraction from Sephadex G-25 chromatography was 0.16 mg/ml. This peptide fraction at 1 mg/ml significantly affected cell cycle progression by arresting P388D1 at G2/M phases. Finally purified peptide from analytical C18 HPLC was nonapeptide of which molecular weight was 1157 Da and the sequence was X-Met-Leu-Pro-Ser-Tyr-Ser-Pro-Tyr.

Lactococcus lactis ssp. lactis ATCC7962의 갈락토tm 및 유당 대사 유전자

이형주 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-

The genes involved in the metabolism of galactose and lactose in Lactococcus lactis ssp. lactis ATCC7962(L. lactis 7962) through Leloir pathway were cloned and they constituted the operon. The gene order reflected the sequential reactions of metabolism of galactose: galA(galactose permease) - galM(aldose mutarotase) - galK(galactokinase) - galT(galactose 1-phosphate uridylyltransferase) - lacA(galactoside acetyltransferase) - lacZ(β-galactosidase) - galE(UDP-glucose 4-epimerase). The lac genes were intervened between galT and galE. Northern blot revealed that galE was constitutively transcribed by its own promoter and induced by several upstream promoters with galactose and lactose. The inducible upstream promoters indicated catabolite carbon repression (CR).

Kluyveromyces lactis에 의한 유청으로부터 과일향 성분의 생성

김소미,이형주 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.5

유당발효 효모인 Kluyveromyces lactis ATCC 8585를 이용하여 유청에서 과일향 풍미성분의 생산성을 향상시키기 위하여 3차에 걸쳐 NTG를 처리하고 geraniol의 항균력에 내성을 지니는 변이주 중 모균주에 비해 yeasty-flavor는 약하나 fruity-flavor가 강한 변이주를 선발하고 K. lactis 450K라 명명하였다. 3일간 배양시킨 발효액을 pentane-dichloromethane(2:1) 용매로 추출하여 얻은 oleoresin을 gas chromatography로 분석한 결과, 모균주보다 변이주가 더 많은 종류와 양의 향기성분을 생산한 것으로 나타났다. 변이주의 배양액으로부터 잠정적으로 확인된 주요향기성분은 테르펜류로는 myrcenol 등이, 알콜류로는 cis-3-hexenol, n-hexanol, 에스테르류로는 ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate, 케톤류로는 methyl vinyl ketone, 기타 성분으로 vanillin, 3-methylcoumarin 등이었다. To enhance the productivity of fruit flavor compounds from whey by the lactose fermenting yeast, Kluyveromyces lactis ATCC 8585 was treated with N-methyl-N'-nitro-N-nitro-soguanidine(NTG). After the NTG treatments, a mutant showing resistance to antifungal activity of geraniol, and strong fruity but low yeasty flavor was selected and named as K. lactis 450 K. Flavor compounds from 3-day culture broth were extracted with pentane-dichloromethane(2:1) and the concentrated oleoresins were analyzed by gas chromatography. The mutant strain produced more classes and larger amount of flavor compounds than the parent strain. Tentatively identified volatile compounds from the culture of the mutant were: terpenes such as myrcenol; alcohols such as cis-3-hexenol, n-hexanol; esters such as ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate; ketones such as methyl vinyl ketones; other compounds such as vanillin, 3-methylcoumarin.

라덱스 혼입률에 따른 철근콘크리트의 휨파괴 거동특성

정원경,김동호,이주형,임홍범,윤경구 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.A

Reinforced concrete(R/C) is commonly used to structures because they have many merits that compressive strength, economy and so on. However, reinforced concrete has a crack at the tensile section which is due to the relatively lower tensile strength than its compressive strength. Latex modified concrete(LMC) has higher tensile and flexural strength than the ordinary portland cement, due to the interconnections of hydrated cement and aggregates by a film of latex particles. The purpose of this study was to investigate the flexural behavior of reinforced concrete beam with latex modified concrete, having the main experimental variables such as concrete types(ordinary portland cement concrete, latex modified concrete), latex contents(0%, 15%), flexural steel ratios(0.012, 0.0235), and with/without shear reinforcement. The beam of LMC showed considerably higher initial cracking loads and ductility than that of OPC, but, similar to ultimate strength and deflection. This might be attributed to the interlocking of hydrated cement and aggregates by a film of latex particles, water retention due to hydrophobic, and colloidal properties of the latexes resulting in reduced water evaporation. The beam with latex modified concrete could be adopted at field for controlling and reducing the tensile crack due to its higher tensile strength.

통조림의 初溫 및 冷却이 殺菌에 미치는 影響

姜君中,崔相道,李乙熙,閔永鳳,金泰圭,金炯甲,朱玉守 진주산업대학교 1989 論文集 Vol.27 No.-

살균전 통조림 내용물의 온도와 냉각처리 유무가 통조림의 살균에 미치는 영향을 조사하였다. 살균시간을 최소화하고 레토르트안과 통조림냉점의 두 곳에서 온도를 감지하여 치사율의 적산치를 계속적으로 이행할 수 있는 컴퓨터시스템을 이용하였다. 8% 벤토나이트 분산액을 301-5호관에 채우고 반복 실험을 통해서 내용물의 초온의 차이와 냉각수의 처리 유무 공히 살균시간에 뚜렷한 영향을 미쳤다. The effect of initial can temperatures and water cooling treatment on the cans for thermal sterilization was described. The accumulated lethal rate method based on microcomputer-based electronic system has introduced in this experiments to minimize the process time and provide precise temperature measurements at 2 points with continuous calculation of the lethal rate. According to a series of experiments which were performed by using 301-5 cans of 8% bentonite suspension, there was significant effect in accumulated lethal rate initiated in different temperatures and treated water cooling treatment.

라텍스 개질 콘크리트의 강도발현 및 내구특성

윤경구,홍창우,이주형,이종명 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

본 연구는 라텍스 혼입률이 5%, 10%, 15%, 20%로 변화할 때 라텍스 개질 콘크리트의 내구 특성을 평가하는데 있다. 라텍스를 콘크리트에 혼입한 라텍스 개질 콘크리트는 보통 콘크리트의 색상과 성형성, 작업성를 갖으며, 약 20%~35%의 단위수향이 감소된다. 그리고 양생후, 콘크리트는 시멘트수화물과 라텍스 입자의 필름막에 의해 연결된 골재로 구성된다. 일반적으로 라텍스 양이 증가하면 콘크리트의 휨강도는 증가하는데, 이것은 라텍스가 시멘트 수화물과 골재사이의 점착력을 증가시키기 때문으로 판단된다. 라텍스 개질 콘크리트와 보통콘크리트와의 상대투수성을 측정하기 위하여 염소이온투과시험법을 사용하였다. 그 결과 라텍스 개질 콘크리트의 투수성은 보통콘크리트의 투수성보다 매우 낮음을 알 수 있었다. 이것은 라텍스가 미세공극을 충진하고 라텍스 입자의 필름막에 의해 골재와 시멘트수화물이 연결 때문이다. 또한 라텍스가 공극주위로 필름막을 형성하기 때문에 공기량이 투수성에 영향을 미치지 못한다는 것을 알 수 있었다. 보통콘크리트에서는 동결융해저항성을 증가시키기 위해 공기연행제를 필요로 하지만, 라텍스 개질 콘크리트에서는 적절한 양생이 이루어지면 추가적인 공기연행제는 요구되지 않는다. 또한 라텍스 혼입률이 증가함에 따라 추가적인 동결융해저항성의 증가는 나타나지 않았다. This study focused on the investigation of strength development and durability of latex modified concrete(LMC) as the latex content variated such as 5%, 10%, 15%, and 20%. When latex was added to concrete, the workability of LMC was quietly increased, compared to that of conventional concrete. This resulted in the decrease of the required water as 20% to 35% in order to obtain a same slump. In general, increasing the amount of latex decreased compressive strength a little but increased flexural strength quite a lot. This might be due to interconnection by a film of latex particles between hydrated cement and aggregates. This, also, might attribute to bonding between the hydrated cement and aggregates. The rapid chloride permeability test was used to evaluate the relative permeability of latex-modified concretes and conventional concretes. The results showed that the permeability of latex-modified concretes was considerably lower than that of conventional concretes tested, which might be due to the latex filled in voids and interconnections of hydrated cement and aggregates by a film of latex particles. Because of latex formed film surrounding the air void, air contents and voids does not effect to the permeability of latex modified concrete.

Transient receptor potential vanilloid type-1 channel regulates diet-induced obesity, insulin resistance, and leptin resistance

Lee, Eunjung,Jung, Dae Young,Kim, Jong Hun,Patel, Payal R.,Hu, Xiaodi,Lee, Yongjin,Azuma, Yoshihiro,Wang, Hsun-Fan,Tsitsilianos, Nicholas,Shafiq, Umber,Kwon, Jung Yeon,Lee, Hyong Joo,Lee, Ki Won,Kim, The Federation of American Societies for Experimen 2015 The FASEB Journal Vol.29 No.8

<P>Insulin resistance is a major characteristic of obesity and type 2 diabetes, but the underlying mechanism is unclear. Recent studies have shown a metabolic role of capsaicin that may be mediated <I>via</I> the transient receptor potential vanilloid type-1 (TRPV1) channel. In this study, TRPV1 knockout (KO) and wild-type (WT) mice (as controls) were fed a high-fat diet (HFD), and metabolic studies were performed to measure insulin and leptin action. The TRPV1 KO mice became more obese than the WT mice after HFD, partly attributed to altered energy balance and leptin resistance in the KO mice. The hyperinsulinemic-euglycemic clamp experiment showed that the TRPV1 KO mice were more insulin resistant after HFD because of the ∼40% reduction in glucose metabolism in the white and brown adipose tissue, compared with that in the WT mice. Leptin treatment failed to suppress food intake, and leptin-mediated hypothalamic signal transducer and activator of transcription (STAT)-3 activity was blunted in the TRPV1 KO mice. We also found that the TRPV1 KO mice were more obese and insulin resistant than the WT mice at 9 mo of age. Taken together, these results indicate that lacking TRPV1 exacerbates the obesity and insulin resistance associated with an HFD and aging, and our findings further suggest that TRPV1 has a major role in regulating glucose metabolism and hypothalamic leptin’s effects in obesity.—Lee, E., Jung, D. Y., Kim, J. H., Patel, P. R., Hu, X., Lee, Y., Azuma, Y., Wang, H.-F., Tsitsilianos, N., Shafiq, U., Kwon, J. Y., Lee, H. J., Lee, K. W., Kim, J. K. Transient receptor potential vanilloid type-1 channel regulates diet-induced obesity, insulin resistance, and leptin resistance.</P>

Analytical Integration of Gradually Varied Flow in Open Channel without Varied-Flow Function

Lee. Hyong Joo,Baek. Kyong Oh 한국방재학회 2017 Journal of Disaster Management Vol.2 No.3

The direct-integration method is a general method used to solve analytically the gradually-varied-flow (GVF) equation. This study has summarized previous works to find the direct integration solutions of the GVF equation by using the varied-flow functions (VFF). The GVF solution by the Bakhmeteff-Chow procedure can be obtained with VFF-tables. These VFF-tables needed in the direct-integration method has a drawback caused by the imprecise interpolation of the VFF-values. To overcome this drawback, it can be solved directly the GVF equation without VFF in this study. Of course, in order to avoid calculation complexity, we use the Chezy formula which is the simplest form of the GVF equations in a wide open channel. The analytical solution derived in this study is exactly identical to that by Bresse (1868).