DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

사회환경교육의 교육과정 표준화 모형 및 통합프로그램 개발연구

박석순,최돈형,성효현,허명,강명희,이숙임,남상준 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

This paper presents the development of standardized modules and an integrated program which will be used for social environmental education. The study objectives are to develop modules which are based on various environmental topics, to relate the regional environmental topics to the educational modules and program, and to include the demand of students in the modules and program. Literature and questionary surveys, as well as, module development method were utilized as research tools. The titles of developed modules are "The Green Plant Earth," "Unexpected Resources: Save Resources and Recycle", "Water as the Mother of All Lives", "The Blue Sky with Clean Air", and "Soil as the Home of All Living and Non-living Things". It is hoped that the developed modules and program are utilized as course materials and curriculum for social environmental education in various educational institutes. The study results will be materialized as multimedia in near futher.

유한 요소법의 자동요소생성 프로그램에 관한 연구

박효근,김형석 순천향대학교 부설 산업기술연구소 2001 순천향 산업기술연구소논문집 Vol.7 No.1

If the geometry of the problem is not one of the simplest, it is very difficult and time comsuming to subdivide the domain into a huge number of triangles by hand-made in order to apply Finite Element Method to analysis model. This paper presents a two dimensional automatic mesh generation which can be easily applicable to electromagnetic problem of complex shape for the finite element method. We implemented an automatic mesh generator using Delaunay triangulation in the windows environments.

체중감량 프로그램에 따른 체력의 변화에 대한 연구 : by Obese Female College Students in Seoul

박효정,장도석,장은재 동덕여자대학교 2003 생활과학연구 Vol.8 No.-

The purpose of this study was to investigate the effect of the aerobic exercise with calorie restricted diet on the change of the body composition, health-related fitness and performance-related fitness during 4 weeks. Twelve obese female college students ages from 20 to 24 who participated in this program. Variables were body composition; Fat mass, Fat free mass. Fat percentage, PIBW(Percentage of ideal Body Weight), BMI(Body Mass Index), health-related fitness and performance-related fitness. The results were as following; First. Diet was found to have a significant decrease of 266㎉ in the change of energy intake and exercise was found to have a significant consume of 309.71㎉ in the change of energy expenditure. Second, Weight, BMI, PIBW, percent body fat, fat mass were found to have a significant decrease of 5.02%, 5.01%, 5.25%, 2.58%, and 2.61% in the change of the body composition, respectively. (P>0.05) FFM was also decreased but it was not significantly. Third, Grip strength and Sit-ups were found to have a significant increase of 2.69% and 2.78% in the change of health-related fitness. (P>0.05) Back strength, Sitting trunk flexion and VO2max were found to have increase of 1.47%, 0.35% and 0.75% in the change of health-related fitness. Forth, Whole body reaction time was found to have a significant decrease of 2.62% in the change of performance-related fitness. Vertical jump and Closed eyes-foot balance were found to have a increase of 1.96% and 1.79% in the change of performance-related fitness. As the results, the weight control program demonstrated to have a positive effect in the improvement of the body composition, health-related fitness and performance-related fitness in obese female.

중증 두부 손상 환자의 예후 인자에 대한 임상 분석 : Clinical Prognostic Factors

송준석,하은주,이종수,이승재,박효일 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.2

A clinical analysis was carried out retrospectively with 54 cases of severe head injured patients(Glasgow Coma Scale score 3 to 8) in identify prognostic factors. Unawareness for over 1 month, abnormal pupillary reaction, abnormal motor response pattern, and diffuse head injury with basal cisternal compression prove to be the most reliable predictors. Individual predictions of outcome provide a powerful tool for assessing the relative efficacy of alternative treatments.

뇌 경막하 축농

송준석,하은주,이종수,이승재,박효일 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12

Subdural empyema is relatively uncommon condition. characterized by purulent collection in the subdural space. Having presented a fulminating clinical course with very high morbidity and mortality. it is conconsidered as a neurosurgical emergency. In recent years, the mortality of subdural empyema has significantly diminished due to rapid diagnosis, aggressive antibiotic therapy and timely surgical intervention. The condition remains a diagnostic challenge ; CT scanning cannot be relied upon although the use of intravenous contrast and more modem scanner has improved the diagnostic yield. MRI offers earlier and more accurate diagnosis of computed tomographically suspected lesion. We report cases of subdural empyemas. which were diagnosed with MR and operaton. We treated one case successfully and the other postoperative case unsuccessfully owing to a delayed diagnosis.

노인복지회관의 공간구성현황에 관한 연구

이효원,박산돌,주석중 한국의료복지시설학회 2004 의료·복지 건축 Vol.10 No.2

This study attempts to provide the fundamental materials for space program in terms of planning a senior welfare center by means of examining and analyzing the present conditions of spatial arrangement of each section in senior welfare center for the aged. The results of the study are as in the following. 1) The demand for new service programs corresponding to the improved quality of the elderly and various social desires is relatively on the increase. Each program in senior welfare center for the elderly should be connected in an organic manner and the organization of space should be provided. 2) The section of counseling, the first step when using a welfare center, should be located in the entrance of the building and it has to be closely connected with other section. 3) The section of medical rehabilitation is a place mostly for the elderly in their frail physical condition; therefore, it is effective to use horizontal line. 4) It is important for the room for volunteers in the section for domiciliary welfare to be arranged flexibly with the office or the room for social education, and it is necessary to consider how easy daytime or short-term care is accessible. 5) The space where dynamic programs are progressed in the section of social education should be distinguished from the section of medical rehabilitation or the room for daytime care and the room for short-term care of the section of domiciliary welfare.

수면 부유 방법에 의한 연초 육묘

洪淳達,石泳善,朴孝澤 충북대학교 엽연초연구소 1999 煙草硏究 Vol.17 No.-

Carbonized rice hull, perlite, and vermiculite were mixed and investigated for the probability of tobacco transplant production by means of plug and transfer float system. These seedling bed materials in condition of float system have gaseous phase ranged from 6% to 13% showing a proper growth of tobacco seedling. However compost media mixed with soil, sand, and compost in condition of float system led to the shortage of respiration in root system by very low proportion of gaseous phase skewing a wicked growth of seedling. Absorption of heat by floating water for the daytime increased rhizosphere temperature in condition of float system during the night and low temperature season resulting in better growth of tobacco seedling. Consequently transplant production of tobacco seedling by float system with mixed materials of carbonized rice hull, perlite, and vermiculite was thought to be desirable method for manufacturing production system of transplant considering the effective management of water, nutrients, and temperature of seedling bed materials.

온도변화에 따른 CFRP 적층재의 충격손상에 관한 실험적 연구

양인영,박효균,차천석 조선대학교 동력자원연구소 1997 動力資源硏究所誌 Vol.19 No.1

In this paper, the effects of temperature change (low and high temperature) on the impact damages of CFRP laminates was experimentally studied. Composite laminates used for this experiment are CF/EPOXY orthotropic laminated plates, which have two-interfaces [0˚_6/90˚_6]_s and [0˚₄/90˚₄]_s. and CF/PEEK orthotropic laminated plates, which have two-interfaces [0˚₄/90˚₄]_s. And, this study aims experimentally to present the interrelations between the impact energy vs. impact damages (i.e. delamination area and matrix crack) of CFRP laminates (CF/EPOXY, CF/PEEK) subjected to FOD(Foreign Object Damage) under various temperatures.

ICR계 마우스에서 홍화씨 에탄올 추출물의 급성독성시험

황윤환,박병권,임종환,김명석,송인배,명노일,정상일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

The present study was carried out to investigate the potential acute toxicity of safflower seed by a single oral dose in ICR mice. Twenty animals of each sex were randomly assigned to four groups of 5 rats each and were administered singly by gavage at dose levels of 0, and 2,500 mg/kg bodyweight. Mortalities, clinical findings, and body weight changes were found and complete gross postmortem examinations were performed. Throughout the study period, no treatment-related deaths were observed. There were no adverse effects in clinical signs, body weight, and gross findings at any dose tested. The results showed that the single oral administration of safflower seed did not cause any toxic effect at the dose levels of 2,500 mg/kg in rats and the minimal lethal dose was considered to be over 2,500 mg/kg body weight for both sexes.