DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

구두의 굽높이가 발의 쾌적감에 미치는 영향(Ⅱ) : 구속압을 중심으로 On the pressure to the Shoed foot

朴永得,金孝垠 啓明大學校 生活科學硏究所 1988 科學論集 Vol.14 No.-

This paper deals with the mutual relationships among the pressures to the shoed foot and in both foot within a new shoe and foot within a fifty-hour-used shoe at the standing posture at three different heel-heights. 1) The pressures to the shoed foot at three different heel-heights is very significant at the level of 0.1%. The heal-heights cause pressure at different parts of foot; the 2nd and 3rd toe points in a 3㎝-heel shoe, and the 1st, 4th and 5th toe points in a 7㎝-heel shoe are more pressured than any other parts of foot. 2) The pressure to the shoed foot caused by heel-heights either within a new shoe or within a fifty-hour-used shoe is significant at the level of 5%, 1% and 0.1%. Girth measurment, length measurement and big-toe angle are more influenced than any other measurements in a new shoe and within fifty-hour-used shoe at three different heel-heights. Height measurement is influenced only by heel-heights, and width measurement is influenced; by a new shoe and a fifty-hour-used shoe; the higher the heel-height is, the more increased the the instep-height is and the narrower the shoe width is, which causes much fatigue when compared with bare foot. Therefore, problems concerned with instep height increasement will be a little solved if foot length, joint girth basic measurements, and foot width are careffully applied for a shoe-making.

정치 웹사이트를 대상으로 한 웹 공간 분석

박한우,한지은,최경호,김효동,Kate A. Mirandilla 한국정보사회진흥원 2006 정보화정책 Vol.13 No.1

본 연구의 목적은 필리핀의 정치 시스템 및 선거 과정에 있어 인터넷의 이용, 역할, 영향 등을 살펴봄으로써 사이버 공간에서 전개되는 정치 활동의 온라인 구조를 분석하는 것이다. 구체적으로 말하면, 본 논문은 지난 2004년의 필리핀 총 선거에서 인터넷이 이용되는 다양한 방식을 검토하여 선거에서의 인터넷의 역할을 필리핀의 정치 문화와 연관하여 탐구하였다. 방법론적으로 먼저, 지난 필리핀 선거의 웹 공간을 형성하는 여러 웹사이트들 가운데 92개가 표집되었다. 표본들을 대상으로 정보성 및 참여유도성 피쳐의 분포 현황을 운영자의 유형에 따라 조사하였다. 연구 결과에 따르면, 정당과 비정부기구에서 운영하는 웹사이트들의 역할이 두드러지는 경향이 나타났다. 정당과 비정부기구는 선거와 관련된 뉴스와 온라인 포럼의 운영 등 가장 많은 유형의 피쳐(feature)를 제공하였다. 후보자 웹사이트 또한 유권자들의 참여를 촉진하는 여러 컨텐츠와 서비스를 상당히 제공하고 있었다. 이러한 결과는 필리핀의 인터넷 정치 문화에 영향을 미친 정치 및 사회적 상황과 결부되어 논의되었다.

한국 초기 근대 무용 연구 : 조택원, 최승희의 작품을 중심으로

박연진,김효순,정은주,문희숙 경희대학교 체육과학연구소 2000 體育學論文集 Vol.28 No.-

Many changing mankind's thought and recognition have affected our history, it is same that dance has become a new concept-dance art under the concept that experiment of a new formality and change are creation. Dancer's feeling is exposed through dance is a phenomenon of carrier which independently embodies self. Dance is a harmony between time and space and a harmony made by rhythmic movement and dance composition. Dance is an art expressing feeling and some situations intentionally dr not by moving its body. The way of communication has been changed since the introduction of the New Dance. In traditional dance, there was no sharply defined boundary to divide spectators and performers, that is, the object and the subject of communication. With the New Dance, there established modern theatres, which separated performers from spectators. With the New Dance, the dance itself became interpretive activity. The New Dance remodelled the traditional dance and introduced some element of ethnic dance from other countries. For example, the costume became so diversified in the New Dance that the range of expression also became expanded greatly.

현대 패션에 나타낸 보보스 스타일 : 2001년부터 2003년까지의 컬렉션을 중심으로

박효은,유영선,이신희 한국의류산업학회 2004 한국의류산업학회지 Vol.6 No.5

The purpose of this study is to know outer and inner characteristics of Bobos style regarded the latest fashion and to estimate the characteristics of fashion trend in 21st century and direction of fashion in the future. In terms of contentions of this studs we firstly studied concept, background, general characteristics which had been introduced in Sociology And then, we studied the effect which the style of Bobos had been given by investigating the concept and style of Hippies and Yuppies. Also, we analysed trend of Bobos represented in modern fashion and tried to examine outer and inner features of Bobos through the result of analysis. Bobos can defined as `Bourgeois Bohemian' who have both mundane desire about success and are high-educated elite class in information-oriented society. The fashion styles which had affected Bobos were psychedelic, naturalism, unisex, ethnic, layered, grunge of Hippies in 1960s and traditional, power-look, contemporary-look, sports wear, casual wear of Yuppies in 1980s. As a result of examining features of Bobos style, we can know that outer feature of Bobos style showed a reasonable harmony regardless of formalities and inner features showed a tendency to seek comforts. Also, Bobos style showed various and free styles, textile materials and colors and complex features to transcend the time and region, and culture.

MTT법을 이용한 사람 골육종과 상피암 세포주들에 대한 항암제 감수성 검사

박승오,신효근,김오환,지은정 全北大學校 齒醫學硏究所 1991 전북치대논문집 Vol.9 No.1

Three anticancer agents which are different in time or dosage dependence, as well as in phase specificity namely mitomycin and adriamycin from natural products and widely used synthetic drug 5 FU, were evaluated for their chemosensivitivy on the five different cancer cell lines ; Four epidermoid carcinomas originated from larynx, cervix, skin and gut were used together with one osteosarcoma as the target cells of single and combined administration of anticancer drugs. Semiautomated tetrazolium dye assay(MTT) appears to offer an attractive option for chemosensitivity of the head and neck cancers since it is a simple, valid and inexpensive method of assessing chemosensityvity for large sample in a short time. The results obtained from this study were as follows. l. Good correlations were obtained with the results of the MTT test and those of ^3H thymidine uptake assay. 2. LD_50 values of HIST and St.Ca, which showed relatively high doubling time on adriamycin were 30㎍/㎖ and 15 ㎍/㎖ while those of HeLa, HEp-2 and KHOS/NP were 2.1㎍/㎖, 4.8㎍/㎖, and 6.8㎍/㎖ respectively. 3. The LD_50 value of 5-FU five cancer cells on were very high ranging from 15㎍/㎖ to almost indefinite number, which means 5 FU is very resisitant to epidermotd carcinomas or osteosarcoma examined in this study. 4. Mitomycin was relatively effective showing 80% cancer cell killing effect on HeLa, 70% on St.Ca, and 50% on HEp-2 at the high concentrations used. 5. Adriamycin was the most effective showing 90% cancer cell killing effect on KHOS/NP, 98% on Hela, 80% on HEp-2, and St. Ca. The least susceptible cancer cells towa adriamycin was HIST having only 55% cell killing effect at the high concentration. 6. Combined therapy of adriamycin and 5-FU was more effective than single administration in all the cases, examined. Most effective synergism was observed on St. Ca. At the low concentration, showing 21 times higher than each single administration.

Vibrio vulnificus 박테리오 파아지의 특성

오양효,윤소겸,공은진,김민정,김영부,박영민 대한감염학회 1995 감염 Vol.27 No.5

목 적 : 이미 보고되어 있는 파아지들의 경우, 일반적인 형태, 숙주역과 몇 가지의 성질만 연구되어 있으므로 보다 상세한 특성에 대한 연구가 필요한 상태이다. 본 연구에서는 해양으로부터 분리한 V. vulnificus를 숙주균으로 하여 투명한 용균반을 형성하는 파아지를 분리해 낸 다음, 분리된 V. vulnificus 파아지의 특징을 연구하고자하였다. 방 법 : V. vulnificus 를 숙주균으로 하여 해산물로부터, 숙주균에 특이적인 파아지를 분리하고, 농축 정제과정을 거쳐 파이지입자를 순수분리하였다. 파아지의 형태를 전자현미경으로 관찰하였으며, 분리된 파아지의 숙주특이성을 조사하고, 파아지 입자의 물리적 특성을 검토하기 위해, 온도 안정성시험, pH 안정성 시험을 실시하였다. 파아지의 흡착율, 파아지의 흡착율에 미치는 무기염 이온의 영향, 온도의 영향과 pH의 영향을 관찰하였다. 파이지의 일단증식곡선을 구하였으며 토끼 면역과 교차 중화 시험, 파아지 핵산의 특성, 구조단백질의 특성을 조사하였다. 결 과 : 전자현미경관찰결과 본 연구에서 분리한 파아지는 두부와 미부를 가지는 형태로 미부에 꼬리판과 꼬리섬유를 가지고 있었다. 파아지의 물리적성질시험 결과 파아지는 50℃이하의 온도에서 안정하였으며 중성pH영역에서 안정하고 산성과 알칼리 영역에서는 활성을 잃었으며 자외선에는 35초 조사에 50% 정도 불활성화 되었다. 파아지는 약 65%의 흡착율을 나타내었으며 흡착에 ??, ??을 요구하였다. 파아지입자가 안정한 온도와 pH영역에서는 흡착에 영향을 받지 않았으며 일단증식시험 결과 108 PHU/ml의 파아지 입자를 생산하였다. 정제한 파아지를 항원으로 하여 토끼에 면역한 항혈청에 파아지가 중화되었으며 파아지 핵산은 이중나선 DNA였다. 파아지의 구조단백질은 SDS-PAGE polyacrylamide gel 전기영동 결과 2개의 밴드를 나타내었다. 그리고 Western blotting 결과 파아지의 전기영동 결과와 동일한 위치의 밴드에서 항원성을 나타내었다. 결 론 : 인체병원균으로 중요성이 인식되고 있는 V. vulnificus는 해산물의 위생과 국민건강에 직접적인 영향을 미칠 수 있는 세균으로 이에 대한 활발한 연구가 요구되는 실정이다. 파아지의 분리율은 숙주균의 분리율에 의존하므로 숙주균의 해수내 분포에 영향을 미친다고 추측되며, 파아지는 숙주균에 감염되어 숙주균을 용균시키므로 숙주균의 개체수를 조절하여 생태학적 측면에서 중요한 기능을 담당하는 것으로 여겨진다. Background : Vibrio vulnificus causes severe wound infection and life-threatening septicemia. In this study we present the characteristics of V. vulnificus bacteriophage and the basis of pathogenicity study of V. vulnificus related on bacteriophage. Methods : We isolated bacteriophage for V.vulnificus from marine products and examined its physiological and biochemical characteristics, and the ultrastructure by electron microscopy (TEM,SEM). And also, we investigated host ranges, temperature stability, pH stability, inactivation by UV irradiation, DNA isolation and analysis and structural protein analysis of the bacteriophage. Results : The adsorption rate of bacteriophage was above 80% and had a little influence at 10-45℃ and pH5-10. Isolated bacteriophage was used to produce antiserum against rabbits and its antigenicity was investigated by cross-neutralization. Western blot analysis showed the capsid protein of bacteriophage had antigenicity. The bacteriophage had double-strand DNA. In restriction analysis, the phage DNA was digested HinfI. The structural protein of phage showed two bands of 34.7kDa and 18.4kDa on SDS-PAGE. Conclusion : The specificity of bacteriophage may be due to the specific binding site that be possessed by host strain surface. Therefore, it seems that virulent bacteriophage controls the number of host strain and then takes a role on natural marine environment.

한국 화교 및 화교 이동에 관한 연구

李效再,朴銀瓊 이화여자대학교 한국문화연구원 1981 韓國文化硏究院 論叢 Vol.37 No.-

The population of Korean Chinese in 1979 was 30,117, among which 94% were from the Shantung peninsula. Compared to the Southeast Asian Chinese, the society of Korean Chinese are a homogeneous ethnic group which uses the same language, came from the same area, and follows the ideology of anti-communism as directed by the Taiwan government. 78% of Korean Chinese are engaged in restaurant businesses, and the others are engaged in general merchandise retailing, Chinese herbal medicine, Chinese herb shops, tearooms, public bath businesses, and tailor shops. In general, job and business opportunities open to Chinese are very limited in Korea. The major organization of Chinese society is " the Chinese Central Association" to which fifth-one local associations belong. Chinese associations play a middleman role between Chinese people and the Chinese embassy in Seoul by reporting people's opinions to the embassy and sending messages from the embassy to the people. Other voluntary organizations are restaurant business associations, local and surname associations, religious and educational associations, and so on which add up to twenty. Most of the voluntary organizations are usually linked to one another through interlocking relations. Thus, leaders in a Chinese community play multiple roles in different associations. Most Chinese children attend Chinese schools which consist of one year of kindergarten, six tears of primary school, and six years of high school. As of 1980, there are nationwide one kindergartens, thirty-nine primary schools, and five high schools. The years of 1882 saw the beginning of Chinese immigration to Korea which was the result of a treaty on trade between Korea and China. Although there were only 209 Chinese in 1883, The Chinese population in Korea reached the level of 82,600 people in 1942. After 1945, however, the division of the country forced two-thirds of the Chinese to live in the northern part of the peninsula. The drop of Chinese immigration after the communalization of mainland China in 1949 also contributed to the decrease of the Chinese population in South Korea along with the government's ban on any immigration into the country. Since then the Chinese population increased only by natural growth, and it reached 32,980 in 1972. In the 1970's, the Chinese population slowly decreased to reach the level of only 29,600 in 1980. The decrease of the Chinese population in the 1970's has been the result of migration of Chinese people to foreign lands. In order to find the trend of their actual mobility, the statistics of Chinese entry-exit have been reviewed. In 1967 the number of entries was 2,600 and the number of was 2,800. Since 1970 it shows a sharp increase and, in 1977, the number of Chinese entering the country was 18,300 and the number leaving the country was 20,00. In other words, the Korean Chinese changed their way of life by traveling back and forth frequently, and some of them even live in Taiwan, visiting Korea once a year only to renew residenceship. These people can be categorized as 'mobile population'. The number of reentry for residence in 1979 was 6,638 which is 22% if the Korean Chinese. If we assume that the average Chinese goes overseas and comes back twice a year, it can be estimated that 11% of Koran Chinese belong to the category of 'mobile population'. In addition, the number of visas issued by the Chinese consulate in Seoul, the number of entries and exits of overseas Chinese to Taiwan, and the number of graduates from Taiwan colleges and junior colleges were also analyzed to prove the high mobility of Korean Chinese in the 1970's. Most of the Korean Chinese who moved out of Korean go to Taiwan and to the United States of America. In this paper, only migration to Taiwan has been analyzed. Interviews were conducted during the period of January and February of 1980 with fifty one Koran Chinese who reside in Taiwan. Life in Taiwan for these emigrants was proven not to be that of a comfortable and leisurely nature. Instead, Korean Chinese have found themselves in a category of a 'minority in their fatherland, i.e. the Korean Chinese formed their own small society by getting together by themselves, having certain common stores they shop, and having little chance to make new Taiwanese friends. The reasons are that either they are too busy keeping stores or small restaurants, or they spend most of their time seeing each other. Moreover, those who do not have any jobs usually engage in a kind of 'shuttle trade', which is conducted by traveling between Korea and Taiwan. Traveling between the two countries, Chinese carry items like herbal drugs, jewels made of ivory, coral, and jade, tapestries, toys and so on from Taiwan to Korea, and blankets, bed spreads, clothes, dresses, ginseng products, and others from Korea to Taiwan. This shuttle trade has become possible by the well systematized process including institutions like drug stores, jewelry stores, and gift shops in Taiwan, and many middleman who purchase the carried items. The store managers in Taiwan take all the carried items from Korea, as well as supplying items to be carried to Korea. Most Chinese migrate to Taiwan as individuals rather than as a whole family. Many Chinese families in Korea end up having their members spread out in different countries, becoming a 'multinational family'. Among the fifty one cases studied, thirty (58%) went to Taiwan for study. Among thirty cases, only seven were attending colleges at the time of study, the rest having been settled in Taiwan after gradation or dropping out of school. The tread is likely to continue that Korean Chinese students entering Taiwan colleges will end up setting in Taiwan more or less permanently. Chinese students in Korea are encouraged by teachers and parents to apply to colleges in Taiwan rather than applying to Korean colleges. One of the major reasons is the way they were educated in primary textbooks under a Chinese curriculum, and are taught by Taiwan colleges educated teachers. The Korean governments liberal educational police for foreigners helped to create the above situation. Therefore Chinese students are well exposed to Chinese culture and society before they go to Taiwan. The strong ethnic identity achieved by pure Chinese education surely induced many Chinese students to apply to Taiwan colleges and to settle in Taiwan. Moreover, other policies of the Korean government in the areas such as naturalization and financial aid, are very restrictive toward Chinese. Curiously enough, a seemingly contradictory policy of permissive education and restrictive naturalization and commerce has maintained a strong ethnic among Chinese people. As a result Chinese began to search for an alternative to give their ethnic identity not a weakness but a pride. Since being a Chinese is a weakness in Korea, Chinese people moved out, leaving their Korean hometowns. Korean Chinese society has a basic group identity as H. Isaacs remarked in "Basic Group Identity: The Idols of the Tribe" ( in Ethnicity : Theory and Experience, edited with an introduction by N, Glazer and Daniel P, Moynihan, 1975). "The Chinese group in Korea is not used as a political or economic unit un order to get ethnic interest as Glazer, Mpynihan and Patterson indicated in the same book. Rather Chinese identity became an ideology in that society. As Aronson insisted, Chinese identity in Korea operates as a leading ideology among Chinese people in Korea. Being one of the first exploratory researches on Korean Chinese, this study has taken a general approach. Future research should deal with more specific subjects such as interethnic relationships between Koreans and Chinese, intermarriages between them, assimilation patterns, the different value systems between the first, second, and third generation Chinese and other anthropological issues. And since many Chinese moved to the United States, a follow-up study of Korean Chinese in the U.S. will be an interesting cross-cultural study.

정책결정과정에 있어서 참여자의 역할 : On the Health Insurance Program 의료보험제도를 중심으로

배철효,박은미 韓國福祉行政學會 1999 복지행정논총 Vol.9 No.-

치매방지 작업기구 사용이 항산화효소 활성에 미치는 영향

조경혜,박정은,윤지윤,문세보,박효영,정인옥,이정원 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

We developed an occupational therapy tool to maintain and improve the cognitive function for the elderly who have senile dementia. 86 elderly people took a mininal mental state examination (MMSE) for the therapy to perform the occupational therapy, and divided into three groups based on avarage ages and MMSE values. The occupational therapy was treated three times per week for 10 weeks. The results showed that there were significant differences in the performing scores before and after the treatment ; the scores for the occupational therapy were increased significantly (p<0.001) and the performing time was shortened from 6-week throughout 10-week regimen. This study was to examine the effect on the activity of antioxidant enzymes by occupational therapy. The activities of antioxidant enzymes such as catalase, Glutathion-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) were increased to the normal standard by the occupational therapy for the elderly having dementia. The recovery of activities of antioxidant enzymes was obvious in severe demented elderly compared to those in normal or mild demented elderly. These results suggest that the occupational therapy had an beneficial effect on the protection against the oxidative stress especially for severe demented elderly, and the occupational tool need to be modified so as to improve the protection against the oxidative stress for normal and/or mild demented elderly.