http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
[PD-0017] Mutation of GmIPK1 Gene using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
Hye Jeong Kim(Hye Jeong Kim),Ji Hyeon Song(Ji Hyeon Song),Gilok Shin(Gilok Shin),Hyeonjin Song(Hyeonjin Song),Ji Eun Lee(Ji Eun Lee),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Jeong, HyeonJin,Jeon, Dae-Woo,Kim, Jin-Ho,Lee, Young Jin,Lee, MiJai,Hwang, Jonghee,Lee, Jungsoo,Yang, Yunsung,Youk, Sookyung,Park, Tae-Ho,Shin, Dongwook The Korean Ceramic Society 2016 한국세라믹학회지 Vol.53 No.2
The effect of zirconium dioxide ($ZrO_2$) on the properties of color conversion glasses was examined in the $BaO-ZnO-B_2O_3-SiO_2$ system. The difference in refractive index between glass and phosphor affect the optical properties of the color conversion glass because of light scattering. Reducing the difference in refractive index is a method to improve the luminous efficacy of color conversion glasses. As a reference, a type of glass that contains 25 mol% of each component was used. To increase the refractive index of the glass samples, the BaO content was increased from 25 to 40 mol%, and $ZrO_2$ was added at levels of 1, 3, and 5 mol%. Color conversion glasses were prepared by sintering a mixture of glass and 5 wt% $YAG:Ce^{3+}$ phosphor. As a result, the refractive index of the glass was found to be dependent on the BaO and $ZrO_2$ contents in the BaO-ZnO-$B_2O_3-SiO_2$ system. As the BaO and $ZrO_2$ contents were increased, the luminous efficacy of the color conversion glass was improved because the refractive index difference between the glass and the $YAG:Ce^{3+}$ phosphor decreased.
Kang, Hyo Jeong,Oh, Ji-Hye,Chun, Sung-Min,Kim, Deokhoon,Ryu, Yeon-Mi,Hwang, Hee Sang,Kim, Sang-Yeob,An, Jihyun,Cho, Eun Jeong,Lee, Hyeonjin,Shim, Ju Hyun,Sung, Chang Ohk,Yu, Eunsil Elsevier 2019 Journal of hepatology Vol.71 No.1
<P><B>Background & aims</B></P> <P>The immunogenomic characteristics of hepatocellular carcinomas (HCCs) with immune cell stroma (HCC-IS), defined histologically, have not been clarified. We investigated the clinical and molecular features of HCC-IS and the prognostic impact of Epstein-Barr virus (EBV) infection.</P> <P><B>Methods</B></P> <P>We evaluated 219 patients with conventional HCC (C-HCC) and 47 with HCC-IS using <I>in situ</I> hybridization for EBV, immunohistochemistry, multiplex immunofluorescence staining, and whole exome and transcriptome sequencing. Human leukocyte antigen types were also extracted from the sequencing data. Genomic and prognostic parameters were compared between HCC-IS and C-HCC. <I>Results:</I> CD8 T cell infiltration was more frequent in HCC-IS than C-HCC (mean fraction/sample, 22.6% <I>vs.</I> 8.9%, false discovery rate q <0.001), as was EBV positivity in CD20-positive tumor-infiltrating lymphocytes (TILs) (74.5% <I>vs.</I> 4.6%, <I>p</I> <0.001). <I>CTNNB1</I> mutations were not identified in any HCC-IS, while they were present in 24.1% of C-HCC (<I>p</I> = 0.016). Inhibitory and stimulatory immune modulators were expressed at similar levels in HCC-IS and EBV-positive C-HCC. Global hypermethylation, and expression of PD-1 and PD-L1 in TILs, and PD-L1 in tumors, were also associated with HCC-IS (<I>p</I> <0.001), whereas human leukocyte antigen type did not differ according to HCC type or EBV positivity. HCC-IS was an independent factor for favorable recurrence-free survival (adjusted hazard ratio [aHR] 0.23; <I>p</I> = 0.002). However, a subgroup of tumors with a high density of EBV-positive TILs had poorer recurrence-free (aHR 25.48; <I>p</I> <0.001) and overall (aHR 9.6; <I>p</I> = 0.003) survival, and significant enrichment of CD8 T cell exhaustion signatures (q = 0.0296).</P> <P><B>Conclusions</B></P> <P>HCC-IS is a distinct HCC subtype associated with a good prognosis and frequent EBV-positive TILs. However, paradoxically, a high density of EBV-positive TILs in tumors is associated with inferior prognostic outcomes. Patients with HCC-IS could be candidates for immunotherapy.</P> <P><B>Lay summary</B></P> <P>Hepatocellular carcinomas with histologic evidence of abundant immune cell infiltration are characterized by frequent activation of Epstein-Barr virus in tumor-infiltrating lymphocytes and less aggressive clinical behavior. However, a high density of Epstein-Barr virus-positive tumor-infiltrating lymphocytes is associated with inferior prognostic outcomes, possibly as a result of immune escape due to significant CD8 T cell exhaustion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EBV positivity in CD20-positive tumor-infiltrating lymphocytes was present in over 70% of HCC-IS. </LI> <LI> HCC-IS lacked <I>CTNNB1</I> mutations and exhibited increased global DNA hypermethylation. </LI> <LI> Both PD-1 and PD-L1 in tumor-infiltrating lymphocytes, and PD-L1 in tumor cells were overexpressed in HCC-IS. </LI> <LI> HCCs with high EBV positivity, paradoxically, were associated with a poor prognosis. </LI> <LI> HCCs with high EBV positivity exhibited increased levels of functionally exhausted CD8 T cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
박현진(Hyeonjin Park),윤소정(Sojeong Yoon),정향연(Hyangyeon Jeong),반영란(Younglan Ban),홍성준(Seong Jun Hong),조성민(Seong Min Jo),이양봉(Yang Bong Lee),신의철(Eui-Cheol Shin) 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.12
본 연구는 다양한 측정 기구를 통해 담마검의 연화점을 측정한 연구로써, 현행 시험법으로 활용되고 있는 ring and ball 방법을 기본 원리로 하여 수동 승온법, 자동 승온법, 자동측정법으로 각각 연화점 측정을 진행하였다. 세 가지 방법을 통한 연화점 측정은 analytical method validation을 통해 타당성을 제시하였으며, 서로 다른 세 곳의 분석기관에서 동시 분석을 진행하여 기관에 따른 오차나 분석일차, 그리고 분석자와 같은 차이에도 타당성을 가진 결과가 확인되었다. 본 연구에서 진행한 실험을 통해 얻은 담마검의 연화점은 수동 승온법에서 77.41℃, 자동 승온법에서 87.20℃, 자동측정법에서 87.33℃로 확인되었다. 식품첨가물공전에 제시되어 있는 담마검의 연화점인 86.00~90.00℃와 비교하였을 때, 수동 승온법에서 나타나는 차이는 소재의 순도(food grade가 아닌 일반용 샘플)의 차이가 연화점 물성에 영향을 주었을 것으로 판단된다. 수동 측정에서 end-point를 결정하는 실험자의 판단에 따른 오차가 주요한 원인으로 판단되며, 승온의 일정한 속도 역시 중요한 요소로 판단되었다. 따라서 본 연구의 연화점 분석 결과, 수동 승온법보다 일정한 온도 승온 방식이나 end-point를 결정하는 방법에 있어 좀 더 표준화된 방법을 도입하는 것이 적합하다고 판단된다. This study was conducted to suggest the most appropriate method for determining the softening point of dammar gum. The softening point value of dammar gum is presented in the Food Additives Code. To determine the softening point, the test was conducted using the ring and ball method for manual-heating, automatic-heating, and automatic-measurement presented in the Food Additives Code of Korea. Analytical method validation was conducted to ensure a precise measurement of the softening point. The softening point of dammar gum was 77.41℃ with a %RSD of 0.22∼5.59% in the manual-heating method, 87.20℃ with a %RSD of 0.43∼1.68% in the automatic-heating method, and 87.33℃ with a %RSD of 2.33% in the automatic-measurement method. The results of this study confirmed the precision of the experimental methods. It was confirmed that the automatic-heating method and automatic-measurement method, which provided results within the range (86.00∼90.00℃) of the softening point value of dammar gum provided in the current Food Additives Code, are appropriate for softening point analysis.
초등학교 플립드 러닝의 효과 분석: 학업성취도와 교과 태도를 중심으로
정민 ( Jeong Min ),김현진 ( Kim Hyeonjin ) 한국교육정보미디어학회(구 한국교육정보방송학회) 2016 교육정보미디어연구 Vol.22 No.3
본 연구는 플립드 러닝이 초등 학습자의 수학 교과 학업 성취도와 태도에 미치는 영향을 알아보고자 하는 데에 그 목적이 있다. 이를 위해 B광역시 A, B 초등학교 6학년 5개 학급 128명을 연구 대상으로 선정하여 이 중 3개 학급에는 플립드 러닝을, 2개 학급에는 전통적 학습 환경을 총 2주간 5차시에 걸쳐 수학 교과에서 각각 실시하였다. 또한 사전 기초학력진단 검사 결과를 통해 상위 30%와 하위 30% 학습 집단을 각각 선정하여 학업 수준에 따른 학습 효과를 분석하였다. 사전과 사후 측정도구는 학업 성취도 검사, 수학 교과에 대한 태도 검사를 활용하였다. 연구 결과, 학습 환경에 따른 학업 성취도 결과와 학업 수준에 따른 학업 성취도 결과 차이는 유의하지 않았다. 플립드 러닝으로 학업성취 향상의 효과를 얻기 위해서는 플립드 러닝의 교실 수업 활동은 학업 성취도 향상을 직접 지원하도록 설계되어야 하며, 온라인 학습 환경은 학습자 수준과 특성에 맞게 설계하고 적극적인 온라인학습 참여를 독려해야 할 것이다. 반면, 학습 환경에 따른 수학 교과에 대한 태도 결과 차이와 학업수준에 따른 수학 교과에 대한 태도 차이는 유의하였다. 이는 플립드 러닝의 적용으로 초등 학습자의학습 태도를 개선하고 흥미를 증진하는 효과를 기대할 수 있음을 시사한다. 본 연구 결과를 바탕으로 본 연구에서는 심층적인 플립드 러닝의 효과 분석을 위해 플립드 러닝 학습 과정과 산출물을 질적 분석할 것, 다양한 학습자 변인에 따른 효과를 다면적으로 분석할 필요가 있음을 제안하였다. The current study aims to identify the effects of Flipped Classroom on the academic achievement and attitude of elementary school students towards mathematics. Subjects of the study include 128 sixth graders in five classes in the A and B elementary schools located in Busan. Among them, three classes were applied with Flipped Classroom, and the remaining two classes were classified into the group applied with traditional studying environment. In addition, 38 advanced learners and 38 students with poor academic achievement were selected to analyze the effect of studying based on academic level. The experiment was conducted five times for two weeks. Mixed-ANOVA was implemented to identify the difference of academic achievement according to the learning environment. However, an effect of interaction between groups and also examination periods was not significant. In addition, two-way ANOVA was performed to identify the difference of academic achievement depending on the academic level in both learning environments, and it was confirmed that there was no significant different in two groups. Mixed-ANOVA was implemented to identify the difference of attitude on the math course according to the learning environment, and an effect of interaction between groups and examination period was significant. Furthermore, two-way ANOVA was performed to identify the difference of attitude on the math course according to the academic level in both learning environments, it was confirmed that there was a difference between two groups, and an effect of interaction between two groups was significant. Implications based on the results of this study include that it is expected to witness an effect of increasing the interest and improving the attitude of elementary school students on the learning by applying Flipped Classroom. In addition, it is needed to discuss the environment design for Flipped Classroom that can improve academic achievement of learners. Lastly, it is required to design Flipped Classroom online studying environment according to the level and features of learners promoting them to actively participate in the online learning.