젤라틴 제조시 추출온도가 젤라틴의 물리적 특성에 미치는 효과

염건웅,이미연,민상기,이성,이혜정 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 실험에서는 돈피를 원료로 acetic acid에 산침한 후, 열수 온도를 달리하여 추출해 낸 젤라틴의 점도, 겔강도, 색도 등의 물리적인 성질을 비교하였다. 산침 후 무게는 원료무게의 2배 이상 증가하였으며 팽윤율은 평균 115.9%로 증가하였고, 산침 후 두께는 평균 2.02mm, 두께의 팽윤율은 152.6%로 증가하였다. 추출율은 온도에 따라서 60℃<100℃<80℃<121℃ 순으로 나타났으며 추출온도별로 비교하면 동일한 온도, 동일한 전단속도에서 점도는 121℃<100℃<60℃<80℃ 순으로 높게 나타나 고온에서 추출하였을 경우 점도가 낮아짐을 알 수 있었다. L-value는 100℃에서 74.78로 가장 높게 나타났으며 121℃에서 가장 낮은 51.96을 보여주었다. a-value는 80℃에서 -1.35로 가장 낮은 값을 나타내었으나 60℃, 100℃, 121℃에서는 유의차가 없었다. b-value는 100℃에서 6.46으로 가장 높게 나타났으며, 80℃에서 3.78으로 가장 낮은 값을 나타내었으나 60℃와 121℃간에는 유의성이 발견되지 않았다. Gel 강도측정 결과를 보면 견고성(hardness)은 121℃<100℃<60℃<80℃순으로 크게 나타났으며, 응집력(cohesiveness)은 추출 온도별로 유의성을 나타내지 않았고 평균 0.66을 나타내었다. 탄성력(elasticity)역시 평균 0.977g/cm²로 추출온도별 유의성은 나타나지 않았다. 부서짐성(brittleness)은 견고성과 마찬가지로 121℃<100℃<60℃<80℃순으로 80℃가 가장 높았다. 그리고 부착성(adhesivenss)의 경우 추출온도별 유의성은 100℃와 121℃에서는 나타나지 않았고, 60℃와 80℃에서만 나타났다. 따라서 추출온도가 낮을수록 견고성과 부착성이 높은 것으로 나타났다. The physical properties of gelatin extracted from pork skin were investigated by determmation of expanding rate of skin, viscosity, gel strength and color depending on extraction temperature. Pork skin during acid treatment was expanded to about 152.6%. The extract rate was increased by 60℃<100℃<80℃<121℃. Viscosity of extracted gelatin sol was increased by 121℃<100℃<60℃<80℃. L-value was highest at 100℃(74.78) and lowest at 121℃(51.96). a-value was lowest at 80℃(-1.35), but there were no significant difference between temperatures of 60, 100 and 121℃. b-value was highest at 100℃(6.46) and lowest at 80℃(3.78). Hardness of gel was increased by 121℃<100℃<60℃<80℃ and cohesiveness was about 0.66 whereas elasticity was about 0.977g/cm³, Brittleness was increased by 121℃<100℃<60℃<80℃, and it was highest at 80℃, which showed similar trend with hardness. Adhesives were no significance difference between 100 and 121℃. From these results, it was achieved that gelatin becomes hard and adhesive when the extraction temperature is low.

cDNA Microarray를 사용하여 Hepatotoxicity 를 유도하는 thioacetamide의 유전자 발현 분석

염혜정,황승용 한양대학교 이학기술연구소 2004 이학기술연구지 Vol.7 No.

Microarray 기술은 단일 hybridization에서 매우 광대하게 유전자의 발현을 profiling할 수 있다. 그리고 Microanay 기술은 독성과 관련하여 생물학적 systems을 위한 강력한 분자유전적 기술로서 사용되어지고 있다. 우리가 중점을 두고 있는 부분은 rat 간에서 hepatotoxicity와 관련이 있는 유전자 발현의 결정을 위해서 독성학의 사용이다. 우리는 본 연구를 위해 Apotosis와 Necrosis에 관련이 있는 Thioacetamide라는 독성물질을 사용하였다. 5-6주 된 수컷 Sprague-Dawely VAF+ albino 3마리 rats에 각 화합물을 24hour와 2weeks를 처리하였다. 독성물질이 처리된 Rat의 간에서 total RNA를 추출 하였고 CDNA microarray를 위해 형광물질(Cy3, Cy5)을 합성했다. 4.8K CDNA microarray를 제작하여 유전자 발현 profiling을 하였다. 우리는 Apotosis와 necrosis와 관련된 유전자 발현의 양상을 볼 수 있었다. Microarray technology, which is massive parallel gene expression profiling in a single hybridization experiment, has provided as a powerful molecular genetic tool for biological system related toxicant. Here we focus on the use of toxicogenomics for the determination of gene expression analysis associated with hepatotoxicity in rat liver. We used in toxicants one compounds, Apotosis and Necrosis; Thioacetamide; for our study. Male Sprague-Dawely VAF+albino 3 rats of 5-6 weeks old were treated with each compound for 24 h and 2 weeks. Total RNA isolated from Rat liver that treated toxicants compounds was used to synthesis of fluroescene for cDNA microarray. 4.8K CDNA microarray in house has been used for gene expression profiling. We found that the level of gene expression related necrosis and Apotosis.

Hepatotoxicity를 유도하는 thioacetamide의 in vivo와 in vitro간에 발현 profiling 비교

염혜정,허영선,황승용 한양대학교 이학기술연구소 2005 이학기술연구지 Vol.8 No.-

Microarray 기술은 단일 hybridization에서 매우 광대하게 유전자의 발현을 profiling할수 있다, 그리고 Microarray 기술은 독성과 관련하여 생물학적 systems을 위한 강력한 분자유전적 기술로서 사용되어지고 있다, 우리가 중점을 두고 있는 부분은 rat간과 cell line(WB-F344)에서 hepatotoxicity와 관련이 있는 유전자 발현의 결정을 위해서 독성학의 사용이다. Thioacetamide는 hepatotoxic를 사용하여 thioacetamide에 의해서 in vivo와 in vitro에서 특이적으로 발현되는 유전자를 profiling했다, 5~6주된 남성 rat(Sprague-Dawely VAF+albino)3마리와 WB-F344 cell line에 24시간 동안 thioacetamide(500㎎/㎏ and 5mM and 10mM)를 처리하였다, 전체 RNA는 thioacetamide가 처리되어진 rat의 간에서 추출하였고 cDNA microarray 기술을 사용하기 위해서 형광물질과 합성하였다, 직접 만든 4.8KcDNA microarrays는 유전자 발현을 profiling하는데 사용되어졌다. 게다가 in vitro에서는 in vivo에서 관찰되어진 유전자 발현과 유사한 성향을 가지고 있음을 보여주었다. Microarray technology which is massice parallel gene expression profiling in a single hybridization experiment has provided as a powerful molecular genetic tool for biological system related toxicant Here we focus on the use of toxicogenomics for the determination of gene expression analysis associated with hepatotoxicity in rat liver and cell line(WB-F344). Thioacetamide is a hepatotoxic compound that affects liver metabolism inhibits mRNA rtansport and induces immune suppression We carried out DNA microarray analyses to assess thioacetamide-specific expression profiles in vivo and in vitro. Male Sprague-Dawelt VAF+ albino 3 rats of 5-6weeks old and WB-F344 cell line were treated with a dose (500㎎/㎏ and 5mM and 10mM) of thioacetamide for 24h Total RNA isolated from Rat liver that treated thioacetamide was used to synthesis of fluorescence for cDNA microarray. 4.8K cDNA microarray in house has been used for gene expression profiling. Furthermore in vitro systems appeared similar in their ability to reproduce thioacetamide-induced changes in gene expression observed in vivo

PDMS와 유리를 이용한 제한효 반응용 마이크로바이오칩 개발

허영선,염혜정,조철호,안유민,황승용 한양대학교 이학기술연구소 2005 이학기술연구지 Vol.8 No.-

최근 생화학 심험 분야에서 초미세 구조를 이용한 소형화 분야가 두드러지게 나타나고 있다. 소형화의 가증 큰 이점은 적은 양의 시료가 사용되기 때문에 실험 비용이 줄어들 뿐 아니라 실험 시간이 단축 된다는 것이다. 게다가 하나의 칩으로 바이오 실험의 모든 단계가 수행되는 랩 온어 칩의 통합을 위한 가능성을 나타내어 준다, 우리는 제한 효소 반응 실험에 응용하기 위해 새로운 마이크로바이오칩을 개발하였다, 이 마이크로 바이오칩은 PDMS와 유리를 사용해 제작하였고 총 실험 반응 양의 10㎕이다, 마이크로바이오칩을 이용한 제한효소 실험은 일반적으로 행해지는 실험 방법에 비해 반응 시간과 시료 양을 단축하는데 성공적이었다. In recent years, significant advances emerged in the area of miniaturzation of biochemical experiment using micro fabricated structures The key sdvantages of the miniaturization would be reduces assay costs due to the low consumpation of expensive reagent and increased experiment speed The additional benefits can be intergrated into Lab-on -a chip in which all the steps necessary in bio assays is performed in a single chip We have developed a novel Microbiochip for th application of Restriction Enzyme cut reaction The Microbiochip with reaction volum of 10 ㎕was fabricated using PDMS/glass The Restriction Enzyme cut reaciton using the microbiochip was successfully done with less time sample compared to the conventioal way

흰쥐의 Adjuvant 유발 다발성 관절염에 대한 마황 약침의 치료 효과

이한창,염미정,김건호,심인섭,최강덕,이혜정,함대현 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2

The current studies investigated the therapeutic effects of Ephedra sinica Stapf (ES) herb-acupuncture on the inflammatory responses of rat arthritic joint, which was induced by the intradermal injection of heat-killed Mycobacterium tuberculosis emulsified in squalene to the base of the tail. The measurements of body weight and articular index were exploited as the assessment methods addressing arthritic symptoms, and the expression profiles of cytokines such as TNF-α, IL-1β and IL-6 in the, rat joint were analyzed using RT-PCR. The articular indexes of arthritic rats were significantly restored after the treatment with ES herb-acupuncture. Although the clinical symptoms of arthritic rats were apparently alleviated by the ES treatments, their body weights were not recovered. It maybe due to the weight-loss and energy enhancement effects of ES extracts. The expression of TNF-α, IL-1β and IL-6 enes, which were highly stimulated in the knee joints of arthritic rats, were restored to the levels of normal rats after the ES treatment. The therapeutic effect of ES herb-acupuncture was not observed in ES-treated, non-acupoint arthritic group as a sham control. The ES herb-acupuncture into an acupoint ST36 was found to be effective in alleviating the arthritic symptoms in adjuvant-induced arthritis rats as regards the body weight, joint appearance and the expression profiles of inflammatory cytokines.

Plyometric Training과 비타민 C 투여가 남고생의 체력, 혈중 젖산, 암모니아 및 젖산탈수소효소에 미치는 영향

장준원,김세종,이현미,서혜림,조현숙,고정림,염종우,예정복,백영호 釜山大學校 附設 體育科學硏究所 2003 體育科學硏究所 論文集 Vol.19 No.-

The purpose of this was to examine the effect of plyometric training and vitamin C ingestion on physical fitness and fatigue substance....

[PD-0016] Reverse genetic study on saddle seed color phenotype of soybean seed via introduced mutation of Argonaute5 gene by CRISPR/Cas9

Hye Jeong Kim(Hye Jeong Kim),In Ah Kim(In Ah Kim),Gilok Shin(Gilok Shin),Wan Woo Yeom(Wan Woo Yeom),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0046] Overexpression of IbMYB1a gene induced anthocyanin accumulation in soybean seeds

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Hye Jeong Kim(Hye Jeong Kim),Hae Gyun Park(Hae Gyun Park),Hye Min Park(Hye Min Park),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Poster Session 1 : Genomics and Bioinformatics Proteomics Chemical Genetics / Genomics ; Gene expression analysis of toxicant induced hepatotoxicity using cDNA microarray

( Hye Jung Yeom ),( Jin Wook Jung ),( Won Ho Ji ),( Jun Ho Maeang ),( Moon Ju Oh ),( Joon Suk Park ),( Yong Soon Lee ),( Kyung Sun Kang ),( Gyoung Jae Lee ),( Wan Seon Lee ),( Ki Seon Jeon ),( Yang Su 한국생화학분자생물학회 (구 한국생화학회) 2004 생화학분자생물학회 춘계학술발표논문집 Vol.2004 No.-

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom, Hye-Sun,Suh, Joon-Hyuk,Youm, Jeong-Rok,Han, Sang-Beom Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.5

Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis$^{(R)}$ Express C18 column (4.6 mm ${\times}$ 150 mm, 2.7 ${\mu}m$) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at $30^{\circ}C$. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 - 200 ${\mu}g$/mL for pulsatilloside E, anemoside $B_4$ and cussosaponin C, and 5 - 500 ${\mu}g$/mL for pulsatilla saponin H with correlation coefficient ($R^2$) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 - 0.2 and 2 - 5 ${\mu}g$/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra- and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.