Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

노인 구강건강관리 실태에 대한 연구

김수화,권순복 경복대학 2002 京福論叢 Vol.6 No.-

본 연구는 고령화사회에 접어든 우리나라의 노인구강건강을 증진시키기 위한 기초자료를 마련하고자 서울특별시에 거주하는 60세 이상 노인을 대상으로 일반사항과 구강상태, 구강보건인식도, 치과진료 및 구강보건교육 경험도에 대한 설문을 실시하여 다음과 같은 결론을 얻었다. 1. 조사대상자는 모두 177명으로 여자 81.4%, 남자 18.6%였고, 연령은 70-74세 27.7%, 65-69세·75-79세가 각각 19.8% 였으며, 학력은 무학이 49.2%로 가장 많았고 초등학교 졸업이 36.2%로 나타났다. 거주형태는 자녀와 함께 사는 경우가 58.2%, 혼자(부부) 사는 경우 41.8%였으며, 직업은 무직인 경우가 90.4%로 많았고, 전신질환은 58.2%가 가지고 있지 않았다. 2. 조사대상자의 구강 내 증상은 저작장애가 64.4%로 가장 많았고, 잔존치아 개수는 25개 이상이 26.0%, 계속가공의치는 대상자의 56.5%가 장착하고 있었고, 대상자의 56.4%가 구강건강이 좋지 않다고 인지하고 있었다. 의치필요자는 총 66.7%, 의치장착자는 총 44,1%로 필요한데 장착하지 못한 노인이 33.9%였다. 잇솔질 횟수는 하루 2회가 50.8%, 시기는 아침식사 후 85.9%, 저녁식사 후 76.3%, 방법은 좌우로 38.4%, 위아래 20.9%, 세치제는 치약이 80.8%로 많았다. 치실과 스케일링 인지도는 각각 16.9%, 36.2%로 나타났고, 음식물제거는 이쑤시개 사용이 46.3%, 치실 3.4%, 구강병대처법은 치과내원이 62.7%로 조사되었다. 최근 1년동안 치과방문경험도는 45.2%, 무면허진료와 구강보건교육 경험도는 46.9%, 18.1%였으며, 우선적으로 필요한 치료는 의치와 보철이 각각 26.6%, 22.6%로 많았다. 3. 연령이 높을수록 의치장착도와 필요도는 높게, 계속가공의치 장착도는 낮게 나타났으며, 저작장애는 연령이 증가함에 따라 크게, 잔존치아 개수는 연령이 증가하면서 감소하는 것으로 나타났다. 치실과 스케일링에 대한 인지도는 연령이 증가함에 따라 낮았고 혼자(부부)사는 노인이 무면허진료 경험도가 56.8%로 높았다. 구강건강이 약하다고 인식할수록 무면허진료 경험도가 높게 나타나 유의한 차이를 보였다. This Thesis was made to offer the data for oral health of the old and to improve their oral health. The subjects of this study were male and female old people who were older than 60 and have lived in Seoul. The questionnaire was made up of population sociological characteristics, oral condition, recognition of oral hygiene and experience of treatment by unlicensed and oral health instruction. The results were as follows : 1. The subjects consisted of 81.4% female and 18.6% male, 27.7% were 70 to 74 in age, 49.2% of the surveyees had never gone to school. In living type 58.2% of the subjects have lived with their children, the unemployed are 90.4% and 58.2% of the subjects had no general disease. 2. 64.4% of the surveyees had masticatory dysfunction, the rate of remained teeth is 26.0% in over 25. 56.4% responded their oral health is weak. The proportion of people wearing denture was 44.1% of the people who need denture and 33.9% in need of denture had no denture. In survey on toothbrushing, 50.8% brushed their teeth twice a day, 85.9% brushed after breakfast and 38.4% used their toothbrush up and down. The recognition of a dental floss and scaling is 16.9% and 36.2% respectively. 46.3% used toothpick in the way to clean food debris and 45.2% visited dental clinic within a year. The rate of experience of the treatment by unlicensed and oral health instruction is 46.9% and 18.1% respectively. 3. The rate of wearing denture, need for denture and masticatory dysfunction is higher and recognition of floss and scaling is lower at the older subjects. The old living alone(coup1e) had higher rate of experience of treatment by unlicensed than the old living with their children.

骨盤腔內 巨大 橫紋筋肉腫 1 例

金瑩洙,河泰原,朴滿洙,姜瞬求,李重禾,尹栗老,薛鍾求 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

Rhabdomyosarcoma, which is a very rare and highly malignant disease in urologic field, has three categories-pleomorphic, alveolar, embryonal, and may arise from any part of the body that contains embryonal mesenchyme, whether striated muscle tissue is present or not. The common site in the genitourinary tract are prostate, bladder, and vagina. Herein we experienced a rhabdomyosarcoma of the pelvic cavity and reviewed literature briefly.

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

우리 나라 온천관광지의 발달궤적과 그 성격의 유형화에 관한 연구

김천중,임화순 龍仁大學校 1998 용인대학교 논문집 Vol.16 No.-

This study aims of identifying the historical development trace and classifying the characters of spa-resorts(20 cases) since 1876 untll the modern era in Korea. The main findings of this study, on the historical development trace and the transition changes of the economic, social situation and the rise and fall of spa utilization, are the classification of the 4 epochs, consisting of 5 periods; (i)Korean traditional utilization spas (ii) Japanese utilization and development spa (iii) Post-Liberation-stagnant period (iv) Revival of spas utilization (v)Active development period. Following these, 3 primary factors ; (a) Favorable Locations (b) Utilization spheres (c) Activities Facilities; are identified, and divided into 16 categories. The quantitative analyses findings on the 16 categories, are the 4type of formation (A, B, C, D). Subsequently, major of the spas start off, are of the A and D type, while the changes from type A to B are also found.

ATM 망에서의 VDT 제공 방안

김영선,김화종,정명순 강원대학교 정보통신연구소 1997 정보통신논문지 Vol.1 No.-

VDT was proposed in order to provide residential multimedia services such as digital video, voice, data and so on, We studied a VDT network architecture and MPEG-2 transmission scheme over an ATM network. For this purpose, we studied the network architecture and communication protocols for MPEG-2 over AAL 5. In a hierarchical video coding, a picture can be seperated into the basic and the higher layers. The basic layer coding is the minimal information needed to reconstruct the video, and the higher layer coding is used to make better quality. By simulation, we obtained the cell loss probability caused by the overflow of video traffic, when subscribers received MPEG-2 video through an ATM network.

Epidermal Growth Factor(EGF)가 개의 손상된 슬관절 전방십자인대의 치유에 미치는 영향

김용식,정도현,정진화,우영균,임영욱,이화성,권순용 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

水分活性을 달리한 白米變溫貯藏中 蔗糖의 變化

金武男,鄭連和,全順實 新羅大學校 1987 論文集 Vol.24 No.-

Free sugars are known to be effective to the quality changes of foods including brown pigment formation through amino-carbonyl reaction and also known to play an important role in the genetic or biological mechanism of formation and breakdown of carbohydrates. Furthermore, the change of free sugars are directly related to the cooking quality of foods containing large amount of carbohydrates. The present study was designed to utilize a kinetic approach to analyze the effect of temperature and water activity on the sucrose decrease in rice. The rice was controlled at a?? of 0.44, 0.52, 0.65, 0.75 using saturated salt solutions and then stored at 30, 40 and 50℃. Another portion of the sample of which the water activities were controlled was stored 30 and 50℃ alternately with 7days interval. Simplified kinetic models were used to obtain the various kinetic parameters for sucrose decrease in rice subjected to accelerated shelf-life tests(ASLT). The reaction of sucrose decrease was first order. The activation energies calculated from Arrhenius plot ranged 5.61-12.86 Kcal/moles and Q?? values were between 1.33 and 1.94. These kinetic parameters were then used to predict starch quality loss under the non-steady storage. Assessed from the above parameters the shelf-lives at 25℃, time to reach 50% loss in sucrose ranged 63 days to 300 days and showed decrease higher than in a??. The predicted shelf-lives at water activities tested were a little with increase actual values.

나체 및 춘추복 착용시의 피부온도와 온열감각과의 관계

김정숙,양가연,최순화 대구산업정보대학 1994 논문집 Vol.8 No.-

The purpose of this study was to obtain the basic data for thermo-physiologically sound wear. The study investigated the skin temperature, rectal temperature, subjective thermal sensation and comfort according to various thermal envirnments. Adult female in twenties was studied in naked body and autumn clothing. The significance of the various factors was analyed according to the environment temperature, maner of dressing. The results were as follows : 1. With a comfort mean skin temperature of 33℃ , naked body was found to be comfortable at environment temperature 28℃, while autumn attire was most comfortable at environment temperature 22℃. 2. Psychological responses of the autumn cloth showed 'hot' 'slightly uncomfortable' as ambient temperature go up. 3. The between thermal sensation and rectal temperature was correlated. 4. From the result of a negative correlation between mean skin temperture and thermal sensation, comfort we can obtain the regression equation for thermal sensation.