Radiological gastrostomy: A comparative analysis of different image-guided methods

Hong-Tao Hu,Hang Yuan,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Hong-Tao Cheng,Jun-Li Ma,Yan Zhao,Li Jiang,Yu-Qing Zhao,Hai-Liang Li 소화기인터벤션의학회 2021 International journal of gastrointestinal interven Vol.10 No.2

Background: Radiographic guided percutaneous gastrostomy has become a safe and effective enteral nutrition method for patients who can not eat by mouth. Fluoroscopy, computed tomography (CT) and cone-beam CT have been routinely used clinically. The aim of this study was to compare the advantages and disadvantages of percutaneous gastrostomy using different radiographic guided methods. Methods: We retrospectively analyzed the clinical data of 538 patients undergoing percutaneous gastrostomy in our department. According to the image guidance method used in gastrostomy, the patients were divided into groups A by fluoroscopy guidance, group B by fluoroscopy combined with C-arm CT guidance, and group C with the whole process CT guidance. The gastrostomy success rate, complication rate, procedure time, and patient radiation dose were analyzed in the three groups. Results: Among 538 patients, 534 were successful and the success rates are 94.3%, 99.3%, and 100% in group A, B, and C, respectively (P > 0.05). There were 3 cases occurred postoperative bleeding as serious adverse events and transferred to surgical gastrostomy. The minor complications include local infection, hyperplasia of granulation tissue, tube obstruction or prolapse, and local pain of the ostomy. The minor complication rates were 10.5%, 10.4%, and 7.7% in group A, B, and C, respectively (P > 0.05). The average procedure time was 25.57 ± 5.99 minutes, 29.01 ± 6.63 minutes, and 45.47 ± 8.98 minutes, respectively (χ2 = 87.98, P < 0.001). The average radiation dosage was 27.30 ± 19.27 mGy, 145.07 ± 106.08 mGy, and 2,590.26 ± 1,088.22 mGy, respectively (χ2 = 204.44, P < 0.001). Conclusion: There were no significant differences in the success rates and complication rates of gastrostomy under the three guiding methods. For difficult cases, CT-guided gastrostomy may be a very useful supplemental method.

Radiological gastrostomy: A comparative analysis of different image-guided methods

Hong-Tao Hu,Hang Yuan,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Hong-Tao Cheng,Jun-Li Ma,Yan Zhao,Li Jiang,Yu-Qing Zhao,Hai-Liang Li 소화기인터벤션의학회 2021 Gastrointestinal Intervention Vol.10 No.2

Background: Radiographic guided percutaneous gastrostomy has become a safe and effective enteral nutrition method for patients who can not eat by mouth. Fluoroscopy, computed tomography (CT) and cone-beam CT have been routinely used clinically. The aim of this study was to compare the advantages and disadvantages of percutaneous gastrostomy using different radiographic guided methods. Methods: We retrospectively analyzed the clinical data of 538 patients undergoing percutaneous gastrostomy in our department. According to the image guidance method used in gastrostomy, the patients were divided into groups A by fluoroscopy guidance, group B by fluoroscopy combined with C-arm CT guidance, and group C with the whole process CT guidance. The gastrostomy success rate, complication rate, procedure time, and patient radiation dose were analyzed in the three groups. Results: Among 538 patients, 534 were successful and the success rates are 94.3%, 99.3%, and 100% in group A, B, and C, respectively (P > 0.05). There were 3 cases occurred postoperative bleeding as serious adverse events and transferred to surgical gastrostomy. The minor complications include local infection, hyperplasia of granulation tissue, tube obstruction or prolapse, and local pain of the ostomy. The minor complication rates were 10.5%, 10.4%, and 7.7% in group A, B, and C, respectively (P > 0.05). The average procedure time was 25.57 ± 5.99 minutes, 29.01 ± 6.63 minutes, and 45.47 ± 8.98 minutes, respectively (χ2 = 87.98, P < 0.001). The average radiation dosage was 27.30 ± 19.27 mGy, 145.07 ± 106.08 mGy, and 2,590.26 ± 1,088.22 mGy, respectively (χ2 = 204.44, P < 0.001). Conclusion: There were no significant differences in the success rates and complication rates of gastrostomy under the three guiding methods. For difficult cases, CT-guided gastrostomy may be a very useful supplemental method.

Endoplasmic Reticulum Mediated Necrosis-like Apoptosis of HeLa Cells Induced by Ca²⁺ Oscillation

Hu, Qingliu,Chang, Junlei,Tao, Litao,Yan, Guoliang,Xie, Mingchao,Wang, Zhao Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

Apoptosis and necrosis are distinguished by modality primarily. Here we show an apoptosis occurred instantly, induced by $300\;{\mu}M$ W-7 ((N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride), inhibitor of calmodulin), which demonstrated necrotic modality. As early as 30 min after W-7 addition, apoptotic (sub-diploid) peak could be detected by fluorescence-activated cell sorter (FACS), “DNA ladders” began to emerge also at this time point, activity of caspase-3 elevated obviously within this period. Absence of mitochondrial membrane potential (MMP) reduction and cytochrome c, AIF (apoptosis inducing factor) release, verified that this rapid apoptosis did not proceed through mitochondria pathway. Activation of caspase-12 and changes of other endoplasmic reticulum (ER) located proteins ascertained that ER pathway mediated this necrosis-like apoptosis. Our findings suggest that it is not credible to judge apoptosis by modality. Elucidation of ER pathway is helpful to comprehend the pathology of diseases associated with ER stress, and may offer a new approach to the therapy of cancer and neurodegenerative diseases.

Effect of Mechanical Alloying and Sintering Behavior on the Microstructure and Properties of NbMoTaWRe Refractory High Entropy Alloy

Tao Gu,Li‑Min Wang,Qiang Hu,Xiu‑Bing Liang,Dong‑Xing Fu,Yong‑Xiong Chen,Xin‑Ming Zhao,Yan‑Wei Sheng 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.11

An equiatomic refractory high-entropy alloy (RHEA) NbMoTaWRe is prepared by mechanical alloying (MA) and sparkplasma sintering (SPS). The effects of mechanical alloying and sintering behaviors on the microstructure and propertiesof the RHEA are investigated. After ball-milling for 30 h, the metastable and supersaturated MA powders with the bodycenteredcubic (BCC) structure are obtained. Then, the MA powders are sintered using the SPS method under the sinteringtemperature range of 1700–1900 °C, and the C atoms and WC introduced by the MA process reacts with the metastable andsupersaturated Ta/Nb phase of the MA powers to form the face-centered cubic (FCC) structure (Nb, Ta)C particles alongthe BCC matrix boundaries during the SPS process. The NbMoTaWRe alloy sintered at 1800 °C consisted of BCC matrixand FCC-type (Nb, Ta)C particles has high compactness (porosity fraction is 0.32%), fracture strength (2630 MPa), plasticstrain (6.82%), and hardness (992 ± 20 HV). These excellent properties of this RHEA are mainly attributed to the combinationof multi-effects, including sintering densification, grain refinement strengthening from the refined sizes (3.80 μm) BCCmatrix, precipitation strengthening from the (Nb, Ta)C particles, solid solution strengthening from multi-principal elementsand interstitial solid solution strengthening from C atoms dissolving into BCC matrix.

MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Hu Liangcong,Xie Xudong,Xue Hang,Wang Tiantian,Panayi Adriana C.,Lin Ze,Xiong Yuan,Cao Faqi,Yan Chengcheng,Chen Lang,Cheng Peng,Zha Kangkang,Sun Yun,Liu Guodong,Yu Chenyan,Hu Yiqiang,Tao Ranyang,Zhou 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

MicroRNAs (miRNAs) broadly regulate normal biological functions of bone and the progression of fracture healing and osteoporosis. Recently, it has been reported that miR-1224-5p in fracture plasma is a potential therapy for osteogenesis. To investigate the roles of miR-1224-5p and the Rap1 signaling pathway in fracture healing and osteoporosis development and progression, we used BMMs, BMSCs, and skull osteoblast precursor cells for in vitro osteogenesis and osteoclastogenesis studies. Osteoblastogenesis and osteoclastogenesis were detected by ALP, ARS, and TRAP staining and bone slice resorption pit assays. The miR-1224-5p target gene was assessed by siRNA-mediated target gene knockdown and luciferase reporter assays. To explore the Rap1 pathway, we performed high-throughput sequencing, western blotting, RT-PCR, chromatin immunoprecipitation assays and immunohistochemical staining. In vivo, bone healing was judged by the cortical femoral defect, cranial bone defect and femoral fracture models. Progression of osteoporosis was evaluated by an ovariectomy model and an aged osteoporosis model. We discovered that the expression of miR-1224-5p was positively correlated with fracture healing progression. Moreover, in vitro, overexpression of miR-1224-5p slowed Rankl-induced osteoclast differentiation and promoted osteoblast differentiation via the Rap1-signaling pathway by targeting ADCY2. In addition, in vivo overexpression of miR-1224-5p significantly promoted fracture healing and ameliorated the progression of osteoporosis caused by estrogen deficiency or aging. Furthermore, knockdown of miRNA-1224-5p inhibited bone regeneration in mice and accelerated the progression of osteoporosis in elderly mice. Taken together, these results identify miR-1224-5p as a key bone osteogenic regulator, which may be a potential therapeutic target for osteoporosis and fracture nonunion.

Study on softening behavior of laser welded joint in GH909 alloy

Fei Yan,Tao Zhan,Sang Liu,Zhengwu Zhu,Chunming Wang,Xiyuan Hu 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

Laser beam welding was conducted on GH909 alloy in a solution treatment and aging conditions. The microstructure, mechanical properties, and softening behavior of welded joints were carefully studied using scanning electron microscopy, energy diffraction spectroscopy, and other techniques. Results indicated that γ′-Ni 3 (TiAl)-precipitated phases were produced inside the matrix, and the presence of Laves at grain boundaries restricted grain growth. A sharp reduction in γ′-precipitated phases evidently weakened the average tensile strength and microhardness of the welded joints. Moreover, fractures occurred in the weld zone. Microcracks in the fracture of the joint mainly depended on the competition result between thermal stress and cohesion forces among the grains.

Endoplasmic Reticulum Mediated Necrosis-like Apoptosis ofHeLa Cells Induced by Ca2+ Oscillation

Qingliu Hu,Guoliang Yan,Junlei Chang,Litao Tao,Mingchao Xie,Zhao Wang 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.6

Gap junction blockage promotes cadmium-induced apoptosis in BRL 3A derived from Buffalo rat liver cells

Di Hu,Hui Zou,Tao Han,Junze Xie,Nannan Dai,Liling Zhuo,Jianhong Gu,Jianchun Bian,Yan Yuan,Xuezhong Liu,Zongping Liu 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Gap junctions mediate direct communication between cells; however, toxicological cascade triggered by nonessential metals can abrogate cellular signaling mediated by gap junctions. Although cadmium (Cd) is known to induce apoptosis in organs and tissues, the mechanisms that underlie gap junction activity in Cd-induced apoptosis in BRL 3A rat liver cells has yet to be established. In the present study, we showed that Cd treatment decreased the cell index (a measure of cellular electrical impedance) in BRL 3A cells. Mechanistically, we found that Cd exposure decreased expression of connexin 43 (Cx43), increased expression of p-Cx43 and elevated intracellular free Ca2+ concentration, corresponding to a decrease in gap junctional intercellular communication. Gap junction blockage pretreatment with 18b-glycyrrhizic acid (GA) promoted Cd-induced apoptosis, involving changes in expression of Bax, Bcl-2, caspase-3 and the mitochondrial transmembrane electrical potential (Δym). Additionally, GA was found to enhance ERK and p38 activation during Cd-induced activation of mitogen-activated protein kinases, but had no significant effect on JNK activation. Our results indicated the apoptosis-related proteins and the ERK and p38 signaling pathways may participate in gap junction blockage promoting Cd-induced apoptosis in BRL 3A cells.

Molecular dynamics simulation of tensile behavior of diffusion bonded Ni/Al nanowires

Zhenjiang Hu,Junjie Zhang,Yong Da Yan,Jiuchun Yan,Tao Sun,김동철,강성원 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.1

Interfaces play key roles in determining mechanical properties of materials. In current work we perform molecular dynamics simulations of diffusion bonding to evaluate the effect of temperature on the morphology of the Ni/Al interface and the strength of the diffusion bonded Ni/Al nanowires. The centro-symmetry parameter is adopted to identify defect atoms generated. Simulation results show that the thickness of the Ni/Al interface has strong dependence on the temperature of diffusion bonding. Following uniaxial tension tests indicate that the yield strength of Ni/Al nanowires is smaller than both the single crystalline Ni and Al nanowires, because of the Ni/Al interface acting as dislocation source and the mobilization of pre-existing dislocations at high temperature. It is shown that the mechanical properties of diffusion bonded Ni/Al nanowires strongly depend on the temperature.

Heat transfer enhancement in cold plate based on FVM method and field synergy theory

Xingjun Hu,Yu Liu,Wei Yan,Jinglong Zhang,Jingyu Wang,Wei Lan,Tao Sang,Tianming Yu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5

To optimize the overall heat dissipation performance of the straight channel of a cold plate for lithium battery in vehicles, we used the wavy channel to optimize the structure and uses the face-centered central composite design (FCCCD), which takes the overall thermal-hydraulic performance factor as the response to explore the interaction mechanism of the flow field and temperature field in wavy channel of the cold plate. When the amplitude of the wavy channel is 1 mm and the number of cycles is 4, the overall thermal-hydraulic performance will reach its maximum with an increase of 17.4 % relative to the straight channel. Then, for the coolant, we explored the heat transfer performance of the nanofluid. The heat transfer coefficient of the nanofluid with a volume fraction of 2 % is 117 % higher than that of pure water and does not cause a significant increase in pressure drop.