Vacuum infiltration transformation of non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) with the pinII gene and bioassay for diamondback moth resistance

Zhang, Junjie,Liu, Fan,Yao, Lei,Luo, Chen,Zhao, Qing,Huang, Yubi The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.3

Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is a popular vegetable in Asian countries. The diamondback moth (DBM), Plutella xylostella (L.), an insect with worldwide distribution, is a main pest of Brassicaceae crops and causes enormous crop losses. Transfer of the anti-insect gene into the plant genome by transgenic technology and subsequent breeding of insect-resistant varieties will be an effective approach to reducing the damage caused by this pest. We have produced transgenic non-heading Chinese cabbage plants expressing the potato proteinase inhibitor II gene (pinII) and tested the pest resistance of these transgenic plants. Non-heading Chinese cabbages grown for 45 days on which buds had formed were used as experimental materials for Agrobacterium-mediated vacuum infiltration transformation. Forty-one resistant plants were selected from 1166 g of seed harvested from the infiltrated plants based on the resistance of the young seedlings to the herbicide Basta. The transgenic traits were further confirmed by the Chlorophenol red test, PCR, and genomic Southern blotting. The results showed that the bar and pinII genes were co-integrated into the resistant plant genome. A bioassay of insect resistance in the second generation of individual lines of the transgenic plants showed that DBM larvae fed on transgenic leaves were severely stunted and had a higher mortality than those fed on the wild-type leaves.

Hybrid Model for Renewable Energy and Load Forecasting Based on Data Mining and EWT

Zhang Jinjin,Zhang Qian,Li Guoli,Wu Junjie,Wang Can,Li Zhi 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.3

Accurate renewable resource (RES) and load prediction play key roles in the power grid planning schemes, eff ective dispatch, and stable operation of power systems. The proportions of wind and solar energy continue to increase, leading to wind and light abandonment. Thus, the absorption of wind and photovoltaic power is particularly important. On the basis of accurately predicting load, wind power and photovoltaic output, the accommodation capacity of wind and photovoltaic power is analyzed. The work contains fi ve parts, as follows: (1) empirical wavelet transform (EWT) is used to decompose wind power and load. At the same time, isolated forest (iForest) and fuzzy C-means clustering (FCM) are used to process photovoltaic data. (2) Low frequency and intermediate frequency components of load are predicted by improved random forest (IRF). High frequency component of load is clustered by improved density-based spatial clustering of applications with noise (IDBSCAN). The processing model is selected on the basis of the characteristics of each class sample. Each component of wind power are predicted by IRF. (3) Photovoltaic power of each category is predicted by IRF. (4) Diff erent components of load and wind power data are added. The photovoltaic power forecast data are synthesized according to the time point. (5) The forecast value of load, wind power, and photovoltaic output of a city are comprehensively evaluated by the summarized prediction level indicators. Three accommodation indicators are used for analyzing the accommodation capacity of wind power and photovoltaic. Results show that the forecasting methods of load, wind power, and photovoltaic power can generate better forecasting results than conventional methods. The analysis results of supplementary prediction level and accommodation indices provide reference for eff ective grid dispatching, sustainable, and healthy energy development.

Utilization of electron beam to modulate electron injection over Schottky barrier

Qi Zhang,Junjie Qi,Yunhua Huang,Huifeng Li,Xin Li,Ruoshui Wang,Yue Zhang 한국물리학회 2011 Current Applied Physics Vol.11 No.3

Modulation of electron injection over Schottky barrier was realized by employing electron beam irradiation on the metal/ZnO-nanowire contact. The structure revealed a good response to the illumination at a scanning frequency of 0.1 Hz, which can be enhanced by increasing bias and decreased with the increase of electron beam energy. These phenomena can be attributed to the Schottky barrier formed at the metal/ZnO-nanowire interface which limits the electron transport across the contact. The electron beam irradiation gives rise to high efficiency of electron injection over the barrier, while the interplay between charge discreteness, coherent scattering, and Coulomb interaction may reduce the conductivity.

Insights Into the BR2/PGP1-mediated Patterns for Shoot and Root Growth in Maize Early Seedling Development by Comparative Transcriptome Sequencing

Xiangge Zhang,Lanjie Zheng,Haojun Zhang,Xinrong Huang,Yinghong Liu,Junjie Zhang,Yu-Feng Hu,Hanmei Liu,Guowu Yu,Yangping Li,Yubi Huang 한국식물학회 2019 Journal of Plant Biology Vol.62 No.3

Plant shoot and root growth is regulated by multiplefactors, including the hormone auxin. A maize brachytic2(br2) mutant displays a shortened shoot and root with highauxin concentration in their apexes, where BR2 encodes anauxin transporter P-glycoprotein1 (PGP1). We employedcomparative transcriptome sequencing to explore the BR2/PGP1-mediated patterns for shoot and root growth amongbr2, wild type (WT), and hybrid F1 (br2 × WT). The resultsshow 352 genes in the br2 shoot and 234 genes in the br2root are differentially expressed compared to WT and F1. Zm00001d045203 and Zm00001d031562 were identified astwo auxin-responsive genes in both the shoot and root. TheZm00001d045203 gene is down-regulated in br2 and encodesan Aux/IAA inhibitor involved in the SCFTIR1/AFB-mediatedauxin signaling pathway. The up-regulated Zm00001d031562gene encodes a small GTPase belonging to the Rab/Yptfamily, which takes part in auxin-mediated responses inArabidopsis. Cytology analysis reveals that reduced cellelongation affects the shoot and root growth of br2. TheZm00001d043477 gene is down-regulated and encodes amember of the cellulose synthase protein family, possiblyreducing cell elongation in the br2 shoot and root. Theseresults reveal the specific regulation of auxin actions and cellresponses by BR2/PGP1.

Night Time Front Vehicle Detection by Using Vehicle Taillight and Lane Information

장준걸(Junjie Zhang),김정하(Jung-Ha Kim) 한국자동차공학회 2015 한국자동차공학회 학술대회 및 전시회 Vol.2015 No.11

In this paper presents an algorithm, aims at practical applications, for the high speed processing and performance enhancement of vehicle detection base on NI machine vision. Detecting the vanishing point and only use extracted low part of the vanishing line. Divides the image into 20 pixels and use the HT(Hough Transform) get the lane candidate point. Fitting the candidate point by RANSAC fitting because it’s robust to outlier. At last use the lane coordinate extraction of ROI. Legally vehicle tail light must be red color, so we use the red plane image with simultaneous processing of noise reduction and edge enhancement using the morphology function. And then give Dilation and Erosion eliminating the noise through the process of Morphology. Finally, using the Projection-Histogram information get the center of mass by restricting threshold detects a vehicle in the ROI. The experimental results were verified in logging video.

Modified Wettability of Micro-structured Steel Surfaces Fabricated by Elliptical Vibration Diamond Cutting

Jianguo Zhang,Andreas Rosenkranz,Junjie Zhang,Jiang Guo,Xiaoqing Li,Xiao Chen,Junfeng Xiao,Jianfeng Xu 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Hardened steel is an important material related to the development of modern industry branches. In order to satisfy the adhesion strength as well as the corrosion and wear resistance under extreme conditions, the modification of the wettability of hardened steel has become an important scientific topic. As micro/nano-structured surfaces play an essential role to induce advanced functional surfaces with wettability control, this contribution aims at presenting the feasibility of micro/nano sculpturing of hardened steels by elliptical vibration diamond cutting. The influence of the fabricated micro/nano structures on the resulting wettability is discussed and related to the contact line density as well as asperity heights. In this regard, it has been verified that a pitch value of 12 μm and structural height of 500 nm are the preferred structural parameters to increase the hydrophobicity of the textured steel specimens.

Disturbance observer based non‑singular fast terminal sliding mode control of permanent magnet synchronous motors

Junjie Jiang,Housheng Zhang,Duo Jin,Ao Wang,Longhao Liu 전력전자학회 2024 JOURNAL OF POWER ELECTRONICS Vol.24 No.2

The existence of load disturbance can lead to the instability of permanent magnet synchronous motor (PMSM) speed control systems. Therefore, a non-singular fast terminal sliding mode control (NFTSMC) strategy based on a disturbance observer is proposed in this paper. First, a new exponential reaching law is presented to relate the rate of convergence to the state error and switching function, which achieves an improvement of the system convergence speed while reducing system jitter. At the same time, to eliminate system jitter, a new smooth saturation function is introduced in place of the symbolic function. Second, a non-singular fast terminal (NFT) sliding surface is generated by combining a nonlinear function with a linear funtion, which makes the state error converge rapidly at different stages. Finally, a disturbance observer is built to boost the robustness of the control system by observing the outside disturbance value and feeding it back to the speed controller. The dependability and effectiveness of the proposed control strategy are confirmed by simulation experiments with MATLAB.

High Resolution ISAR Imaging Based on Improved Smoothed L0 Norm Recovery Algorithm

( Junjie Feng ),( Gong Zhang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.12

In radar imaging, a target is usually consisted of a few strong scatterers which are sparsely distributed. In this paper, an improved sparse signal recovery algorithm based on smoothed l₀ (SL0) norm method is proposed to achieve high resolution ISAR imaging with limited pulse numbers. Firstly, one new smoothed function is proposed to approximate the l₀ norm to measure the sparsity. Then a single loop step is used instead of two loop layers in SL0 method which increases the searching density of variable parameter to ensure the recovery accuracy without increasing computation amount, the cost function is undated in every loop for the next loop until the termination is satisfied. Finally, the new set of solution is projected into the feasible set. Simulation results show that the proposed algorithm is superior to the several popular methods both in terms of the reconstruction performance and computation time. Real data ISAR imaging obtained by the proposed algorithm is competitive to several other methods.

Diffusion-controlled adsorption kinetics of aqueous submicellar and micellar solution at air/solution interface in the limit of short time

Junji Liu,Ying Zhang 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.5

γeq of aqueous Pentaethylenglycol-monodecylether (C12E5) solutionsat temperature 25oC were measured by means of Wilhelmy plate method and the determined critical micelle concen-tration (cmc) is 6.8×102 mol·m3. To study adsorption kinetics of aqueous submicellar and micellar solutions, the dy-namic surface tensions γ(t) were measured by means of maximal buble pressure method. Using the derived equa-sorption kinetics was studied. For micellar solution (c0>cmc) of C12E5, the demicellization constant was calculated.

Fluorogenic probes for disease-relevant enzymes

Zhang, Junji,Chai, Xianzhi,He, Xiao-Peng,Kim, Hae-Jo,Yoon, Juyoung,Tian, He The Royal Society of Chemistry 2019 Chemical Society reviews Vol.48 No.2

<P>Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the <I>in situ</I> activity of enzymes in live cells and <I>in vivo</I>. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Specific attention is given to fluorogenic probes that have been developed for analysis of the activities of enzymes including oxidases and reductases, those that act on biomacromolecules including DNAs, proteins/peptides/amino acids, carbohydrates and lipids, and those that are responsible for translational modifications. We envision that this review will serve as an ideal reference for practitioners as well as beginners in relevant research fields.</P>