Circulating MiRNA-21-enriched extracellular vesicles promote bone remodeling in traumatic brain injury patients

Lin Ze,Xiong Yuan,Sun Yun,Zeng Ruiyin,Xue Hang,Hu Yiqiang,Chen Lang,Liu Guodong,Panayi Adriana C.,Zhou Wu,Cao Faqi,Gao Fei,Mi Bobin,Liu Guohui 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Fracture combined with traumatic brain injury (TBI) is one of the most common and serious types of compound trauma in the clinic and is characterized by dysfunction of cellular communication in injured organs. Our prior studies found that TBI was capable of enhancing fracture healing in a paracrine manner. Exosomes (Exos), as small extracellular vesicles, are important paracrine vehicles for noncell therapy. However, whether circulating Exos derived from TBI patients (TBI-Exos) regulate the prohealing effects of fractures remains unclear. Thus, the present study aimed to explore the biological effects of TBI-Exos on fracture healing and reveal the potential molecular mechanism. TBI-Exos were isolated by ultracentrifugation, and the enriched miR-21-5 p was identified by qRT‒PCR analysis. The beneficial effects of TBI-Exos on osteoblastic differentiation and bone remodeling were determined by a series of in vitro assays. Bioinformatics analyses were conducted to identify the potential downstream mechanisms of the regulatory effect of TBI-Exos on osteoblasts. Furthermore, the role of the potential signaling pathway of TBI-Exos in mediating the osteoblastic activity of osteoblasts was assessed. Subsequently, a murine fracture model was established, and the effect of TBI-Exos on bone modeling was demonstrated in vivo. TBI-Exos can be internalized by osteoblasts, and in vitro, suppression of SMAD7 promoted osteogenic differentiation, whereas knockdown of miR-21-5 p in TBI-Exos strongly inhibited this bone-beneficial effect. Similarly, our results confirmed that preinjection of TBI-Exos led to enhanced bone formation, whereas knockdown of exosomal miR-21-5 p substantially impaired this bone-beneficial effect in vivo.

다크투어리즘 스토리텔링이 몰입경험과 재방문의도에 미치는 영향: 방문객의 거주지역에 따른 집단간 차이검증

Ze Lin,양위주 대한관광경영학회 2023 觀光硏究 Vol.38 No.4

Dark tourism is the phenomenon of traveling to sites associated with death, disaster, suffering, violence and terror, and provides tourists with unique tourist experiences. Flow experience generated through storytelling in dark tourism provides optimal tourist experiences and influences tourist behaviors. Understanding the group differences based on the residential areas of visitors contributes to the development of tourism content and tourism promotion strategies. This study aimed to examine the structural relationships among dark tourism storytelling, flow experience and revisit intention, and group difference verification was conducted based on the residential areas of visitors. Chinese tourists who visited the Memorial Hall of the Victims in Nanjing Massacre by Japanese Invaders, China were selected as the survey objects. A total of 254 valid questionnaires were empirically analyzed using structural equation modeling. Firstly, dark tourism storytelling consists of three crucial attributes: emotionality, understandability and educability. Secondly, this research confirmed the significant positive effect of dark tourism storytelling on flow experience and the significant positive effect of flow experience on revisit intention. Thirdly, it was significantly different depending on the residential areas of visitors in the causal relationships between dark tourism storytelling and flow experience. Based on the findings, this study raises theoretical and practical implications for the development of dark tourism and provides an important basis for decision-making to revisit dark tourism destinations. 다크투어리즘은 죽음, 재난, 고통, 폭력, 공포와 관련된 관광목적지를 방문하는 현상으로 독특한 관광경험을 제공한다. 다크투어리즘에서 스토리텔링을 통해 생성된 몰입경험이 최적의 관광경험을 제공하여 관광행동에 영향을 미친다. 방문객의 거주지역에 따른 집단간의 차이를 이해하면 관광 콘텐츠를 개발하고 관광 진흥 전략을 수립하는 데에 기여할 수 있다. 본 연구는 다크투어리즘 스토리텔링, 몰입경험, 재방문의도 간의 인과관계를 실증적으로 분석하였으며 방문객의 거주지역에 따른 집단간의 차이를 검증하였다. 중국 침화일군 난징 대학살 조난동포 기념관을 방문한 경험이 있는 중국 국적 관광객들을 대상으로 설문조사를 실시하였으며 수집된 254부 유효한 설문지를 사용하여 구조방정식모델을 통해 분석하였다. 연구결과에 따르면 첫째, 다크투어리즘 스토리텔링은 정서성, 이해용이성, 교육성 3개 구성요인이 도출되었다. 둘째, 다크투어리즘 스토리텔링이 몰입경험에 유의한 긍정적인 영향을 미칠 뿐만 아니라 몰입경험이 재방문의도에 유의한 긍정적인 영향을 미치는 것으로 나타났다. 셋째, 다크투어리즘 스토리텔링과 몰입경험의 관계에서 방문객의 거주지역에 따라 유의한 차이가 있는 것이 검증되었다. 이 연구결과를 바탕으로 다크투어리즘 발전에 대한 이론적 및 실무적 시사점을 제기하며 다크투어리즘 관광목적지의 재방문 의사결정에 중요한 기초자료를 제공할 것이다.

Comparison of Insulin Sensitivity, Glucose Sensitivity, and First Phase Insulin Secretion in Patients Treated with Repaglinide or Gliclazide

Chung-Ze Wu,Dee Pei,An-Tsz Hsieh,Kun Wang,Jiunn-Diann Lin,Li-Hsiu Lee,Yi-Min Chu,Fone-Ching Hsiao,Chun Pei,Te-Lin Hsia 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.3

The traditional sulfonylureas with long half-lives have sustained stimulatory effects on insulin secretion compared to the short-acting insulin secretagogue. In this study, we used the frequently sampled intravenous glucose tolerance test (FSIGT) to evaluate the insulin sensitivity (IS), glucose sensitivity (SG), and acute insulin response after glucose load (AIRg) after 4months treatment with either gliclazide or repaglinide. The design of study was randomizedcrossover. We enrolled 20 patients with new-onset type 2 diabetes (mean age, 49.3 years). Totally three FSIGTs were performed, one before and one after each of the two treatment periods as aforementioned. No significant differences in fasting plasma glucose, insulin, body mass index, blood pressure, glycated hemoglobin, or lipids were noted between the two treatments. After the repaglinide treatment, higher AIRg, lower IS, and lower SG were noted, but they did not reach statistical significance. The disposal index (DI) was also not significantly different between the two treatments. In conclusion, since non-significantly higher DI, AIRg, lower IS and SG were noted after repaglinide treatment, it might be a better treatment for diabetes, relative to gliclazide.

Patient and Care Delays of Breast Cancer in China

Yue-Lin Li,Ya-Chao Qin,Lu-Ying Tang,Yu-Huang Liao,Wei Zhang,Xiao-Ming Xie,Qiang Liu,Ying Lin,Ze-Fang Ren 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose This study differentiates patient and care delays of breast cancer and explores the related factors as well as the associations with the prognosis in Guangzhou, a southern city of China. Materials and Methods A cohort of female incident breast cancer patients (n=1,551) was recruited from October 2008 to March 2012 and followed up until January 1, 2016 (n=1,374) in the affiliated hospitals of Sun Yat-sen University. The factors associated with patient and care delays were analyzed with multivariable logistic models. Cox proportional hazards regression models were constructed to estimate the impacts of the delays on the prognosis. Results There were 40.4% patient delay (! 3 months) and 15.5% care delay (! 1 month). The patient delay, but not the care delay, was significantly related to the clinical stage and consequently worsened the prognosis of breast cancer (hazard ratio, 1.45; 95% confidence interval, 1.09 to 1.91 for progression-free survival). The factors related to an increased patient delay included premenopausal status, history of benign breast disease, and less physical examination. Conclusion Patient delay was the main type of delay in Guangzhou and resulted in higher clinical stage and poor prognosis of breast cancer. Screening for breast cancer among premenopausal women may be an effective way to reduce this delay.

Simulation analysis and evaluation of decontamination effect of different abrasive jet process parameters on radioactively contaminated metal

Zhong Lin,Deng Jian,Zuo Zhe-wen,Huang Can-yu,Chen Bo,Lei Lin,Lei Ze-yong,Lei Jie-heng,Zhao Mu,Hua Yun-fei 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.11

A new method of numerical simulating prediction and decontamination effect evaluation for abrasive jet decontamination to radioactively contaminated metal is proposed. Based on the Computational Fluid Dynamics and Discrete Element Model (CFD-DEM) coupled simulation model, the motion patterns and distribution of abrasives can be predicted, and the decontamination effect can be evaluated by image processing and recognition technology. The impact of three key parameters (impact distance, inlet pressure, abrasive mass flow rate) on the decontamination effect is revealed. Moreover, here are experiments of reliability verification to decontamination effect and numerical simulation methods that has been conducted. The results show that: 60Co and other homogeneous solid solution radioactive pollutants can be removed by abrasive jet, and the average removal rate of Co exceeds 80%. It is reliable for the proposed numerical simulation and evaluation method because of the well goodness of fit between predicted value and actual values: The predicted values and actual values of the abrasive distribution diameter are Ф57 and Ф55; the total coverage rate is 26.42% and 23.50%; the average impact velocity is 81.73 m/s and 78.00 m/s. Further analysis shows that the impact distance has a significant impact on the distribution of abrasive particles on the target surface, the coverage rate of the core area increases at first, and then decreases with the increase of the impact distance of the nozzle, which reach a maximum of 14.44% at 300 mm. It is recommended to set the impact distance around 300 mm, because at this time the core area coverage of the abrasive is the largest and the impact velocity is stable at the highest speed of 81.94 m/s. The impact of the nozzle inlet pressure on the decontamination effect mainly affects the impact kinetic energy of the abrasive and has little impact on the distribution. The greater the inlet pressure, the greater the impact kinetic energy, and the stronger the decontamination ability of the abrasive. But in return, the energy consumption is higher, too. For the decontamination of radioactively contaminated metals, it is recommended to set the inlet pressure of the nozzle at around 0.6 MPa. Because most of the Co elements can be removed under this pressure. Increasing the mass and flow of abrasives appropriately can enhance the decontamination effectiveness. The total mass of abrasives per unit decontamination area is suggested to be 50 g because the core area coverage rate of the abrasive is relatively large under this condition; and the nozzle wear extent is acceptable

rs10505474 and rs7837328 at 8q24 Cumulatively Confer Risk of Prostate Cancer in Northern Han Chinese

Zhang, Lin-Lin,Sun, Liang,Zhu, Xiao-Quan,Xu, Yong,Yang, Kuo,Yang, Fan,Yang, Yi-Ge,Chen, Guo-Qiang,Fu, Ji-Cheng,Zheng, Chen-Guang,Li, Ying,Mu, Xiao-Qiu,Shi, Xiao-Hong,Zhao, Fan,Wang, Fei,Yang, Ze,Wang, Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Aims: Genome-wide association studies (GWAS) have identified several risk variants for prostate cancer (pCa) mainly in Europeans, which need to be further verified in other racial groups. We selected six previously identified variants as candidates and to define the association with PCa in Northern Han Chinese. Methods: 749 subjects from Beijing and Tianjin in Northern China were included. Six variants (rs10505474, rs7837328, rs4242384, rs7813, rs486907 and rs1058205) were genotyped by high resolution melting (HRM) assays. The individual and cumulative contribution for of the risk of PCa and clinical covariates were analyzed. Results: Among the six candidate variants, onlyrs10505474, and rs7837328, both locating at 8q24 region, were associated with PCa in our population.rs10505474 (A) was associated with PCa ($OR_{recessive}=1.56$, p=0.006); and rs7837328 (A) was associated with PCa ($OR_{dominant}=1.38$, p=0.042/$OR_{recessive}=1.99$, p=0.003). Moreover, we observed a cumulative effects between them ($p_{trend}=2.58{\times}10^{-5}$). The joint population attributable risk showed the two variants might account for 71.85% of PCa risk. In addition, we found the homozygotes of rs10505474 (A) and rs7837328 (A) were associated with PCa clinical covariants (age at onset, tumor stage, respectively) ($p_{age}=0.046$, $P_{tumorstage}=0.048$). Conclusion: rs10505474 (A) and rs7387328 (A) at 8q24 are associated with PCa and cumulatively confer risk, suggesting the two variations could determine susceptibility to PCa in the Northern Chinese Han population.

“자전거 보관대” 공공 디자인 계획안

이임택(Li, Lin-Ze),박상준(Park, Sang-Jun),홍관선(Hong, Kwan-Seon) 한국실내디자인학회 2010 한국실내디자인학회 학술대회논문집 Vol.12 No.3

Recently, we are faced with the reality where the space for public places has been decreasing due to emphasis on privacy and the expansion of private paces. However, with the topic about "Public Design," the change from the public possession to public value has been promoted, which means that the design to emphasize publicity of public value rather than private design has been received more attention. Departed from the rapid economical change focusing on quantitative rather than qualitative growth in the past, now qualitative growth with the change of values has been stressed, and along with this, the significance of urbanization, urban design, design of public space and publicity has been granted. Therefore, this study aims to shed new light on the recognition of public design and its change through "Bicycle Rack" design which is the element of public design, according to urbanization and publicity, the stream of the age. The modern society pursues complexation and diversification from unification. Public design also requires diversified forms and designs. Therefore, this design was designed with basic needs for a bench and bicycle rack adding design elements to accord with diversification and multi-purpose. It is used as a bench or bicycle rack depending on personal needs. Likewise, I designed the part of public design for complex use. Therefore, if we use the elements of multipurpose, diversification, complexation properly, it will be remembered as a better street and public design.

Effects of Buffer Extraction of Protein Feeds on In Vitro Fermentation Characteristics, Degradation and Methane Production by Rumen Microbes

Seong-Ho Choi,Guang-Lin Jin,Wei-Ze Qin,Sun-Sik Chang,Joon Jeong,Man-Kang Song 경상대학교 농업생명과학연구원 2015 농업생명과학연구 Vol.49 No.6

The present in vitro study was conducted to examine the effect of buffer solubility of eight protein feeds (coconut meal, distillers grain, sesame meal, perilla meal, soy source cake, rape seed meal, soybean meal and lupine) on the fermentation characteristics, degradability of dry matter (DM) and crude protein (CP), and methane (CH4) production by rumen microbes. Buffer extraction increased pH (P<0.05 ~ p<0.001) of the culture solution but tended to lower ammonia- N concentration for all protein feeds. Total volatile fatty acids(VFAs) and each VFAs concentrations in all incubation was decreased by buffer extraction (P<0.01 ~ P<0.001). Also, molar proportion of acetate in 1h (P<0.001), 3h (P<0.01) and 12h (P<0.05) incubations and molar proportion of propionate in 1h (P<0.001), 3h (P<0.01), 6h (P<0.05) and 12h (P<0.05) were decreased by buffer extraction. But molar proportion of butyrate in 1h (P<0.001), 3h (P<0.01) and 6h (P<0.05) were increased by buffer extraction. The in vitro effective degradability of dry matter (P<0.001) and CP (P<0.001) was decreased by buffer extraction. The methane production (P<0.01~P<0.001) in all incubation was decreased by buffer extraction. The results from in the current study might be useful for diet formulation to improve the feed efficiency of the ruminant animals without massive loss of major nutrients.

In-situ hydrodeoxygenation of furfural to furans over supported Ni catalysts in aqueous solution

Ze Wang,Zhaolin Fu,Weigang Lin,Songgeng Li,Wenli Song 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.8

In-situ hydrodeoxygenation of furfural as a representative component in bio-oil was investigated in aqueous solution over supported Ni catalysts, for preparing furans as an antiknock additive. The addition of methanol, ethanol, or isopropanol was found inhibitive to coke formation at 220 oC. When using methanol as the hydrogen donor and coke inhibitor, the support in mesoporous structure with moderate acidity was more favorable to the conversion of furfural and to the formation of furans. An increased loading amount of Ni facilitated the generation of deep hydrogenated products. The conversion of furfural could hardly be changed under different methanol to water ratios, while the product distribution varied remarkably. Under optimized conditions, the summary yield of furan and 2-methylfuran reached to above 85%. On the basis of optimized reaction conditions, the in-situ hydrodeoxygenation of an eight-component synthetic bio-oil was tested, and the results verified the adaptability of the method for conversion of bio-oil.

Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with Tephrosia purpurea

( Ze Ping Luo ),( Hai Yan Lin ),( Wen Bing Ding ),( Hua Liang He ),( You Zhi Li ) 한국균학회 2015 Mycobiology Vol.43 No.4

Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.