Momordicine I alleviates isoproterenol-induced cardiomyocyte hypertrophy through suppression of PLA2G6 and DGK-ζ

Hongming Li,Yumei Qiu,Mengdie Xie,Changsheng Ouyang,Xiaoyun Ding,Hao Zhang,Wei Dong,Yinhua Xiong,Xilan Tang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

This study aimed to observe the protective effect of momordicine I, a triterpenoid compound extracted from momordica charantia L., on isoproterenol (ISO)-induced hypertrophy in rat H9c2 cardiomyocytes and investigate its potential mechanism. Treatment with 10 μM ISO induced cardiomyocyte hypertrophy as evidenced by increased cell surface area and protein content as well as pronounced upregulation of fetal genes including atrial natriuretic peptide, β-myosin heavy chain, and α-skeletal actin; however, those responses were markedly attenuated by treatment with 12.5 μg/ml momordicine I. Transcriptome experiment results showed that there were 381 and 447 differentially expressed genes expressed in comparisons of model/control and momordicine I intervention/model, respectively. GO enrichment analysis suggested that the anti-cardiomyocyte hypertrophic effect of momordicine I may be mainly associated with the regulation of metabolic processes. Based on our transcriptome experiment results as well as literature reports, we selected glycerophospholipid metabolizing enzymes group VI phospholipase A2 (PLA2G6) and diacylglycerol kinase ζ (DGK-ζ) as targets to further explore the potential mechanism through which momordicine I inhibited ISO-induced cardiomyocyte hypertrophy. Our results demonstrated that momordicine I inhibited ISO-induced upregulations of mRNA levels and protein expressions of PLA2G6 and DGK-ζ. Collectively, momordicine I alleviated ISO-induced cardiomyocyte hypertrophy, which may be related to its inhibition of the expression of glycerophospholipid metabolizing enzymes PLA2G6 and DGK-ζ.

Pricing Range Accrual Notes in an A¢ ne Term Structure Model with Stochastic Mean, Stochastic Volatility and Jumps

Shaoyu Li,Hongming Huang,Li-Chuan Tsai 한국재무학회 2012 한국재무학회 학술대회 Vol.2012 No.09

The main purpose of this paper is to derive the analytic valuation formulae of …xed range accrual notes (FiRAN) and ‡oating range accrual notes (FlRAN) in the context of an a¢ ne term-structure model incorporating stochastic long-run mean, stochastic volatility and jumps according to the empirical …ndings of An- dersen et al.(2004). We propose a quasi-analytic pricing and hedging solutions for range accrual notes and these solutions are demonstrated in sensitivity analysis. Our numerical results show all these three factors signi…cantly a¤ect the values and hedging strategies of range accural notes, and the factor of stochastic mean plays the most important role in either valuation or hedging.

MicroRNA-301b promotes cell proliferation and apoptosis resistance in triple-negative breast cancer by targeting CYLD

( Hongming Song ),( Dengfeng Li ),( Tianqi Wu ),( Dan Xie ),( Kaiyao Hua ),( Jiashu Hu ),( Xiaochong Deng ),( Changle Ji ),( Yijun Deng ),( Lin Fang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.11

Aberrant expression of microRNAs (miRNAs) plays important roles in carcinogenesis and tumor progression. However, the expression and biological role of miR-301b in triple-negative breast cancer (TNBC) remains unclear. Here we aimed to evaluate the roles and mechanisms of miR-301b in TNBC cells. miR-301b expression was assessed in TNBC specimens and cell lines by quantitative Real-Time PCR (qRT-PCR). TNBC cells were transfected with miR-301b mimics, inhibitors or Cylindromatosis (CYLD) small interfering RNA (siRNA) using Lipofectamine 2000. The functional roles of miR-301b were determined by cell proliferation, colony formation, and apoptosis assays. Western blots and qRT-PCR were used to measure the expression of mRNAs and proteins in the cells. We found that miR-301b was upregulated in TNBC specimens and cell lines. Overexpression of miR-301b promoted cell proliferation in TNBC cells, while inhibited the apoptosis induced by 5-FU. CYLD was downregulated by miR-301b at both mRNA and protein levels in TNBC cells. Dual-luciferase report assay confirmed that miR-301b downregulated CYLD by direct interaction with the 3’-untranslated region(3’-UTR) of CYLD mRNA. NF-κB activation was mechanistically associated with miR-301b-mediated downregulation of CYLD. However, inhibition of miR-301b reversed all the effects of miR-301b. In conclusion, miR-301b plays an oncogenic role in TNBC possibly by downregulating CYLD and subsequently activating NF-κB p65, and this may provide a novel therapeutic approach for TNBC. [BMB Reports 2018; 51(11): 602-607]

VGLL4 interacts with STAT3 to function as a tumor suppressor in triple-negative breast cancer

Hongming Song,Qifeng Luo,Xiaochong Deng,Changle Ji,Dengfeng Li,Amik Munankarmy,Wei Jian,Junyong Zhao,Lin Fang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Triple-negative breast cancer (TNBC) is an aggressive malignancy with a poor prognosis, and there are no effective molecular-targeted drugs for TNBC patients in clinical practice. The JAK-STAT pathway is implicated in tumorigenesis and the progression of various cancers. In this study, the results demonstrated that VGLL4 is expressed at low levels in both TNBC specimens and cell lines and that VGLL4 expression is negatively correlated with Ki67 expression and tumor size in TNBC patients. VGLL4 knockdown can promote the growth of TNBC cells, while VGLL4 overexpression significantly suppresses the growth of TNBC cells in vitro. More importantly, VGLL4 significantly inhibits tumor progression in a nude mouse model. In addition, VGLL4 is a direct target of miR-454, and the upregulation of miR-454 decreases VGLL4 expression and promotes the cell growth of TNBC cells. Furthermore, we also demonstrated that VGLL4 interacts with STAT3, the core component of the JAK-STAT pathway, leading to the inactivation of STAT3 and the inhibition of STAT3 downstream transcription. Collectively, these findings indicate that VGLL4 expression is negatively associated with poor prognosis in TNBC patients. High expression of miR-454 may be one of the causes of the downregulation of VGLL4 in TNBC, and VGLL4 acts as a tumor suppressor in TNBC by interacting with STAT3 and subsequently suppresses the STAT3 signaling axis, providing potential biomarkers and therapeutic approaches for this fatal disease.

Orthorhombic carbon oC24: A novel topological nodal line semimetal

Li, Zhen-Zhen,Chen, Jia,Nie, Simin,Xu, Lifang,Mizuseki, Hiroshi,Weng, Hongming,Wang, Jian-Tao Elsevier 2018 Carbon Vol.133 No.-

<P><B>Abstract</B></P> <P>We identify by <I>ab initio</I> calculations a stable three-dimensional carbon allotrope that constructed by inserting benzene rings into the carbon-carbon bonds in a previously reported oC8 carbon. This new carbon phase has a 24-atom orthorhombic unit cell in C m m m ( D 2 H 19 ) symmetry and thus termed oC24. Phonon mode analysis confirms its dynamical stable. Total-energy calculations show that it is energetically stable comparable with (5,5) carbon nanotube. Detailed electronic band calculations reveal that oC24 is a topological node-line semimetal with two mirror-inversion symmetric nodal lines that go through the whole Brillouin zone in bulk and a projected surface flat band around the Fermi level. The present results establish a new type of carbon phases and offer insights into their outstanding structural and electronic properties.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hydrogen sulfide ameliorates abdominal aorta coarctation-induced myocardial fibrosis by inhibiting pyroptosis through regulating eukaryotic translation initiation factor 2α phosphorylation and activating PI3K/AKT1 pathway

Yaling Li,Zhixiong Wu,Jiangping Hu,Gongli Liu,Hongming Hu,Fan Ouyang,Jun Yang The Korean Society of Pharmacology 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.4

This study aimed to assess the effects of exogenous hydrogen sulfide (H₂S) on abdominal aorta coarctation (AAC) induced myocardial fibrosis (MF) and autophagy in rats. Forty-four Sprague-Dawley rats were randomly divided into control group, AAC group, AAC + H₂S group, and H₂S control group. After a model of rats with AAC was built surgically, AAC + H₂S group and H₂S group were injected intraperitoneally with H₂S (100 µmol/kg) daily. The rats in the control group and the AAC group were injected with the same amount of PBS. We observed that H₂S can improve left ventricular function and the deposition of myocardial collagen fibers, inhibit pyroptosis, down-regulate the expression of P-eif2α in myocardial tissue, and inhibit cell autophagy by activating the phosphatidylinositol 3-kinase (PI3K)/AKT1 signaling pathway (p < 0.05). In addition, angiotensin II (1 µM) H9c2 cardiomyocytes were injured in vitro experiments, and it was also observed that pyroptosis was inhibited after H₂S (400 µmol/kg) intervention, the expression of P-eif2α in cardiomyocytes was significantly down-regulated, and the PI3K/AKT1 signaling pathway was activated at the same time. Therefore, increasing the expression of P-eif2α reverses the activation of the PI3K/AKT1 signaling pathway by H₂S. In conclusion, these findings suggest that exogenous H₂S can ameliorate MF in rats with AAC by inhibiting pyroptosis, and the mechanism may be associated with inhibiting the phosphorylation of eif2α and activating the PI3K/AKT1 signaling pathway to inhibit excessive cell autophagy.

Enhanced electrochemical performances of LiNi0.5Co0.2Mn0.3O2 cathode material co-coated by graphene/TiO2

Dang Mengyue,Li Ying,Xu Chaoxiang,He Yulin,Yu Chunpeng,Liu Wenbo,Jin Hongming,Zhu Mingyuan,Zhang Jiujun,Li Wenxian 한국물리학회 2021 Current Applied Physics Vol.32 No.-

The electrochemical performances of LiNi0.5Co0.2Mn0.3O2 (NCM523) layered cathode material, such as poor rate capacity and cycling stability caused by undesirable intrinsic conductivity and low rate of lithium ion transportation, are not fairly good especially at elevated rate and cut-off voltage. To improve these properties, in this study, the co-coating layer of graphene and TiO2 was constructed on NCM523 surface. The graphene/TiO2 coating layer could effectively prevent hydrofluoric acid (HF) attacks, suppress the side reaction, accelerate the lithium ion diffusion and facilitate the electron migration. The enhancement of cycle performance and rate capacity was contributed to the uniform co-modified surface, interacting each other and thus exhibiting synergistic effects.

Simultaneously photocatalytic removal of Cr(VI) and metronidazole by asynchronous cross-linked modified sodium alginate

Shaojie Wu,Mingyang Li,Lili Xin,Hongming Long,Xiangpeng Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Conventional photocatalysts for environmental applications are mainly transitional metal compounds orprecious metal nanoparticles, which suffer from weak pollutant adsorption performance, thus limitedtheir applications for high concentrated wastewaters. In this study, asynchronous cross-linked triethylenetetraminemodified sodium alginate (CFTS) was used as photocatalyst for the simultaneouslycatalytic degradation of Cr(VI) and metronidazole (MNZ). Experimental results suggested that asynchronouscross-linking and triethylenetetramine modification have dramatically enhanced the chemicalstability as well as the adsorption and photocatalytic behavior of the material. The grafted amino functionalgroups and cross-linked Fe3+ ions can promote the rapid separation and transfer of electrons, thusreducing the recombination rate of photo-generated electron-hole pairs. The optimum removal rate of Cr(VI) has reached 99.5 % and MNZ can be completely removed within 20 and 30 min at pH 1.0 under UVlight irradiation, respectively. The removal mechanism of Cr(VI) by CFTS is the synergistic effect of electrostaticadsorption and photocatalytic reduction, while photocatalytic oxidation dominated the degradationprocess of MNZ. Moreover, the removal efficiency can be enhanced in the binary solution ofthese two pollutants due to the utilization of photo generated electron-hole pairs, which provides newpathways in the treatment of complicated pharmaceutical and domestic sewage wastewaters.

Study on the Microstructure of Single Stage Hot Deformed Anisotropic Nd-Fe-B Magnets

Hao Tian,Ying Li,Mingyuan Zhu,Hongming Jin,Dongzhe Jin 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

An Analytical Research about Parameter Influence on Large-Scale Anchorage Region with Multiple CFRP Cables

Bo Feng,Jitao Zhong,Hongming Li 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.2

This study investigates the mechanical properties of carbon fiber reinforced polymer (CFRP) cable at the anchoring region where arrange the adhesive material with different mediums aiming at reducing the high stress concentration. The 3D finite element (FE) model was established based on cable force of 100t and 1000t, and its accuracy was verified by experimental results. The effects of geometrical dimensions and mediums stiffness on anchoring capacity of the cable was studied. The results show that the proposed anchorage gets high anchoring efficiency. The gradient variation of the stiffness of the adhesive material can effectively reduce the stress crest of the cable at the anchorage region. The distributions of the radial and shear stress of the cable at the anchorage region become comparatively flat with the increase in the number of segments. The variation stiffness and length ratio have the main influence on the stress of the cable in three orientations. Friction coefficient and internal taper have the secondary effects on the radial stress and the axial displacement. Anchor length and thickness of adhesive material only affect the radial stress. Anchorage design should primarily focus on the stiffness and length ratio of the adhesive material. The anchoring method proposed in this paper is helpful to anchor multi-tendon cables efficiently, and promote the application of high-capacity FRP cables in large-span spatial structure.