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- 저자 : ( Tianqi Wu ) (검색결과 3 건)
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Mast Cells Tryptase Promotes Intestinal Fibrosis in Natural Decellularized Intestinal Scaffolds
Wan Jian,Wu Tianqi,Liu Ying,Yang Muqing,Fichna Jakub,Guo Yibing,Yin Lu,Chen Chunqiu 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4
BACKGROUND: Standard two-dimensional (2D) culture has confirmed the mechanism of mast cells (MCs) in the pathogenesis of inflammatory bowel disease (IBD), but the regulation of signaling responses of MCs may well differ in three-dimensional (3D) microenvironments. The aim of the study was to develop a 3D culture model based on decellularized intestinal scaffolds (DIS) and verify how MCs influenced fibroblasts phenotype in the 3D model. METHODS: DIS were achieved using the detergent technique and extracellular matrix (ECM) components were verified by histologic analysis, quantification and scanning electron microscope. After human colon fibroblasts recellularized into the scaffolds and activated by MCs tryptase and TGFb1, the changes in genes and signaling pathways during fibroblasts activation in 3D were studied and compared with the changes in 2D cell culture on plastic plates. RESULTS: Decellularization process effectively removed native cell debris while retaining natural ECM components and structure. The engrafted fibroblasts could penetrate into the scaffolds and maintain its phenotype. No matter whether fibroblasts were cultured in 2D or 3D, MCs tryptase and transforming growth factor b1 (TGF-b1) could promote the differentiation of fibroblasts into fibrotic-phenotype myofibroblasts through Akt and Smad2/3 signaling pathways. Furthermore, the pro-collagen1a1 and fibronectin synthesis of myofibroblasts in 3D was higher than in 2D culture. CONCLUSION: Our results demonstrated that the DIS can be used as a bioactive microenvironment for the study of intestinal fibrosis, providing an innovative platform for future intestinal disease modeling and screening of genes and signaling pathways.
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( Hongming Song ),( Dengfeng Li ),( Tianqi Wu ),( Dan Xie ),( Kaiyao Hua ),( Jiashu Hu ),( Xiaochong Deng ),( Changle Ji ),( Yijun Deng ),( Lin Fang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.11
Aberrant expression of microRNAs (miRNAs) plays important roles in carcinogenesis and tumor progression. However, the expression and biological role of miR-301b in triple-negative breast cancer (TNBC) remains unclear. Here we aimed to evaluate the roles and mechanisms of miR-301b in TNBC cells. miR-301b expression was assessed in TNBC specimens and cell lines by quantitative Real-Time PCR (qRT-PCR). TNBC cells were transfected with miR-301b mimics, inhibitors or Cylindromatosis (CYLD) small interfering RNA (siRNA) using Lipofectamine 2000. The functional roles of miR-301b were determined by cell proliferation, colony formation, and apoptosis assays. Western blots and qRT-PCR were used to measure the expression of mRNAs and proteins in the cells. We found that miR-301b was upregulated in TNBC specimens and cell lines. Overexpression of miR-301b promoted cell proliferation in TNBC cells, while inhibited the apoptosis induced by 5-FU. CYLD was downregulated by miR-301b at both mRNA and protein levels in TNBC cells. Dual-luciferase report assay confirmed that miR-301b downregulated CYLD by direct interaction with the 3’-untranslated region(3’-UTR) of CYLD mRNA. NF-κB activation was mechanistically associated with miR-301b-mediated downregulation of CYLD. However, inhibition of miR-301b reversed all the effects of miR-301b. In conclusion, miR-301b plays an oncogenic role in TNBC possibly by downregulating CYLD and subsequently activating NF-κB p65, and this may provide a novel therapeutic approach for TNBC. [BMB Reports 2018; 51(11): 602-607]
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Longitudinal Dynamic End Effect of Single-Sided Linear Induction Motor for Medium–Low Speed Maglev
Wang Ying,Liu Xuelong,Lu Weiguo,Wen Tianqi,Yu Fan,Wu Qian 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.4
The Short Primary Single-sided Linear Induction Motor (SLIM) applied in medium–low maglev vehicle has end eff ect that will cause a signifi cant reduction of train thrust. This paper analyzes the relationship between the Longitudinal Dynamic End Eff ect (LDEE) and the speed of SLIM. It is concluded that LDEE increases with the motor speed. The LDEE at motor’s exit area is called tailing eff ect. We take advantage of the tailing eff ect between adjacent SLIMs to make the air-gap magnetic fi eld of these motors continuous by adjusting their intervals and excitation phase diff erence. The several SLIM on the same side of the vehicle is equivalent to a “whole motor” with overall one entry area and one exit area. This method can reduce the resistance of the whole vehicle running at high speed, which can provide a new solution for the design of high-speed SLIM applied in Maglev
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