Hepatic microRNAome reveals potential microRNA-mRNA pairs association with lipid metabolism in pigs

Jingge Liu,Caibo Ning,Bo-jiang Li,Rongyang Li,Wang Jun Wu,Honglin Liu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.9

Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.

Comparison and Analysis of Meteorological Variables Observed from the Glacier Area over the Tianshan Mountains in Kyrgyzstan and China

Honglin Pan,Wen Huo,Kanike Raghavendra Kumar,Ali Mamtimin,Minzhong Wang,Junjian Liu,Chenglong Zhou,Fan Yang,Xinghua Yang 한국기상학회 2021 Asia-Pacific Journal of Atmospheric Sciences Vol.57 No.2

Due to the special natural environment over the glacier area in the Tianshan Mountains, the observation of meteorological data became difficult and is strictly scarce. Using the available data observed from the automatic meteorological station (AMS) installed on theWestern Tianshan Mountain Station (WTMS) in Kyrgyzstan, the daily and seasonal variations of meteorological variables including air temperature (AT, °C), air pressure (AP, hPa), relative humidity (RH, %), and wind direction and speed (WD and WS, deg and m/s, respectively), as well as total radiation (TR, W/m2) were primarily analyzed during the period from September 2016 to May 2017. These are compared with the meteorological data obtained from the AMS in the Eastern Tianshan Mountain Station (Daxigou Station, hereafter named as ETMS) in China. Further, the comparison study between the observed data from the WTMS and the NCEP/NCAR reanalysis data was performed to test the feasibility of reanalysis data in the vicinity of the Western Tianshan Mountains. The results revealed are as follows: (1) the observed data between the WTMS and ETMS presented high correlations, and the meteorological parameters detected from the ETMS were certainly representative of the variations in theWTMS. (2) The RH in the two regions showed comparatively high values ranging from 40%to 70%indicate the abundant amount of atmospheric water vapor content near the ground in the Tianshan Mountain area. (3) The predominant WD both in the WTMS and ETMS near the ground was along the south-east direction, with the values of WS between 0 and 2 m/s were accounted the largest proportion in two stations. (4) The NCEP/NCAR reanalysis data can reflect the changes in the synoptic weather scale of AT, RH, and AP to some extent. (5) The NCEP/NCAR reanalysis data of AT and RH were underestimated by 7.2% and 45.2% in the WTMS, respectively. Whereas, the values of AP, WD, and WS were overestimated by 83.8%, 28.8%, and 74.3%, respectively over the WTMS.

Static Var Generator Based on Accurate Linearization Combined with Sliding Mode Controller

Liu Fei,Wang Xueyan,Huang Xinyi,Yan Honglin,Huang Xiangdong,Pan Hongguang 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

The active and reactive current are hard to be decoupled completely with strong robustness when the traditional proportional integral (PI) algorithm is adopted to feedforward decoupling control for static var generator (SVG). This paper proposed a compound control strategy for SVG based on precise linearization and sliding mode control (SMC). To be specifi c, fi rst, the theory of diff erential geometry is used to prove that the mathematical model of SVG satisfi es the necessary and suffi - cient condition of precise linearization. Second, the precise linearized high-order system of SVG was transformed into two completely independent decoupled fi rst-order linear systems, and realize complete decoupling of active current and reactive current. Finally, SMC was used to design both the voltage outer loop and the current inner loop. Simulations ware carried out for the SVG mathematical model under three conditions which were inductive load, capacitive load and sudden change load. The results of those simulation showed that the controller designed by the proposed control strategy has the advantages of quick reactive power compensation, strong robustness and anti-interference performance.

20(S)-ginsenoside Rg3 exerts anti-fi brotic effect after myocardial infarction by alleviation of fi broblasts proliferation and collagen deposition through TGFBR1 signaling pathways

Honglin Xu,Haifeng Miao,Guanghong Chen,Guoyong Zhang,Yue Hua,Yuting Wu,Tong Xu,Changlei Hu,Mingjie Pang,Leyi Tan,Xin Han,Bin Liu,Yingchun Zhou 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Myocardial fibrosis post-myocardial infarction (MI) can induce maladaptive cardiacremodeling as well as heart failure. Although 20(S)-ginsenoside Rg3 (Rg3) has been applied to cardiovasculardiseases, its efficacy and specific molecular mechanism in myocardial fibrosis are largely unknown. Herein, we aimed to explore whether TGFBR1 signaling was involved in Rg3's anti-fibrotic effectpost-MI. Methods: Left anterior descending (LAD) coronary artery ligation-induced MI mice and TGF-b1-stimulated primary cardiac fibroblasts (CFs) were adopted. Echocardiography, hematoxlin-eosin andMasson staining, Western-blot and immunohistochemistry, CCK8 and Edu were used to study the effectsof Rg3 on myocardial fibrosis and TGFBR1 signaling. The combination mechanism of Rg3 and TGFBR1 wasexplored by surface plasmon resonance imaging (SPRi). Moreover, myocardial Tgfbr1-deficient mice andTGFBR1 adenovirus were adopted to confirm the pharmacological mechanism of Rg3. Results: In vivo experiments, Rg3 ameliorated myocardial fibrosis and hypertrophy and enhanced cardiacfunction. Rg3-TGFBR1 had the 1.78 10 7 M equilibrium dissociation constant based on SPRi analysis,and Rg3 inhibited the activation of TGFBR1/Smads signaling dose-dependently. Cardiac-specific Tgfbr1knockdown abolished Rg3's protection against myocardial fibrosis post-MI. In addition, Rg3 downregulatedthe TGF-b1-mediated CFs growth together with collagen production in vitro through TGFBR1signaling. Moreover, TGFBR1 adenovirus partially blocked the inhibitory effect of Rg3. Conclusion: Rg3 improves myocardial fibrosis and cardiac function through suppressing CFs proliferationalong with collagen deposition by inactivation of TGFBR1 pathway.

In silico Discovery of Genes Expressed in Liver, Kidney, Spleen and Small Intestine of Pigs

Pan, Zengxiang,Liu, Honglin,Chen, Jie,Xu, Dan,Jiang, Zhihua,Xie, Zhuang Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.2

An in silico approach was developed to survey the genes expressed in four internal organs of pig: liver, kidney, spleen and small intestine. The major procedures of the approach included: (1) BLAST searching against GenBank "est_others" database using human cDNA sequences as queries to screen the porcine orthologous expressed sequence tags (ESTs), (2) classifying the porcine ESTs records by resources according to certain criteria and (3) analyzing data for ESTs specifically expressed in each organ. In order to do so, four Java programs were developed. Based on the ESTs available in the GenBank database, it was found that there were at least 2,100 genes expressed in these four organs, including 128 in the liver, 81 in the kidney, 780 in the spleen, and 1,423 in the small intestine respectively (a few genes co-expressed in these tissues). Gene expression patterns, such as co-expressed genes, preferentially expressed genes and basic active genes were also compared and characterized among these organs. This study provides a comprehensive model on how to use the bioinformatics approach and Genbank databases to facilitate the discovery of new genes in livestock species.

Three sesquiterpene lactones suppress lung adenocarcinoma by blocking TMEM16A-mediated Ca2+-activated Cl− channels

Ruilian Xiu,Jie Jia,Qing Zhang,Fengjiao Liu,Yaxin Jia,Yuanyuan Zhang,Beibei Song,Xiaodan Liu,Jingwei Chen,Dongyang Huang,Fan Zhang,Juanjuan Ma,Honglin Li,Xuan Zhang,Yunyun Geng 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.6

Transmembrane protein TMEM16A, which encodes calcium-activated chloride channel has been implicated in tumorigenesis. Overexpression of TMEM16A is associated with poor prognosis and low overall survival in multiple cancers including lung adenocarcinoma, making it a promising biomarker and therapeutic target. In this study, three structure-related sesquiterpene lactones (mecheliolide, costunolide and dehydrocostus lactone) were extracted from the traditional Chinese medicine Aucklandiae Radix and identified as novel TMEM16A inhibitors with comparable inhibitory effects. Their effects on the proliferation and migration of lung adenocarcinoma cells were examined. Whole-cell patch clamp experiments showed that these sesquiterpene lactones potently inhibited recombinant TMEM16A currents in a concentration- dependent manner. The half-maximal concentration (IC50) values for three tested sesquiterpene lactones were 29.9 ± 1.1 μM, 19.7 ± 0.4 μM, and 24.5 ± 2.1 μM, while the maximal effect (Emax) values were 100.0% ± 2.8%, 85.8% ± 0.9%, and 88.3% ± 4.6%, respectively. These sesquiterpene lactones also significantly inhibited the endogenous TMEM16A currents and proliferation, and migration of LA795 lung cancer cells. These results demonstrate that mecheliolide, costunolide and dehydrocostus lactone are novel TMEM16A inhibitors and potential candidates for lung adenocarcinoma therapy.

Chk2 Regulates Cell Cycle Progression during Mouse Oocyte Maturation and Early Embryo Development

Xiao-Xin Dai,Xing Duan,Honglin Liu,최향순,Nam-Hyung Kim,Shao-Chen Sun 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.2

As a tumor suppressor homologue during mitosis, Chk2 is involved in replication checkpoints, DNA repair, and cell cycle arrest, although its functions during mouse oocyte meiosis and early embryo development remain uncertain. We investigated the functions of Chk2 during mouse oocyte maturation and early embryo development. Chk2 exhibited a dynamic localization pattern; Chk2 expression was restricted to germinal vesicles at the germinal vesicle (GV) stage, was associated with centromeres at pro-meta-phase I (Pro-MI), and localized to spindle poles at meta-phase I (MI). Disrupting Chk2 activity resulted in cell cycle progression defects. First, inhibitor-treated oocytes were arrested at the GV stage and failed to undergo germinal vesicle breakdown (GVBD); this could be rescued after Chk2 inhibition release. Second, Chk2 inhibition after oocyte GVBD caused MI arrest. Third, the first cleavage of early embryo development was disrupted by Chk2 inhibi-tion. Additionally, in inhibitor-treated oocytes, checkpoint protein Bub3 expression was consistently localized at cen-tromeres at the MI stage, which indicated that the spindle assembly checkpoint (SAC) was activated. Moreover, disrupting Chk2 activity in oocytes caused severe chromo-some misalignments and spindle disruption. In inhibitor-treated oocytes, centrosome protein γ-tubulin and Polo-like kinase 1 (Plk1) were dissociated from spindle poles. These results indicated that Chk2 regulated cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development.

Synthesis, characterization and tribological performances of nano-CeO2/biodiesel carbon soot composites as a novel lubricant additive in polyalphaolefin

Chuan Li,Xinxin Wang,Qiangqiang Zhang,Xu Tan,Yefeng Liu,Honglin Li,Hao Liu,Enzhu Hu,Xianguo Hu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

This study fabricated nano-CeO2/biodiesel carbon soot (BCS) composites using the raw material BCS,which is the byproduct of biodiesel combustion and cerium salt and used as a novel lubricant additiveto PAO6 for steel/steel contact. Oleylamine modified nano-CeO2/BCS composites comprise abundant lipophilicgroups, and thus nano-CeO2/BCS composites exhibit good dispersion in PAO6. The tribologicalresults showed that nano-CeO2/BCS composites significantly improved the lubricating performances ofPAO6. In case of 0.1 wt% nano-CeO2/BCS composites dispersed in PAO6, the friction coefficient and wearvolume were reduced by 17.3 and 35.2%, respectively. The improved tribological properties were attributedto the composites rolling and forming of a lubricating film on the friction surface and the presence ofBCS and CeO2 in composites exerting a synergistic lubricating effect, which simultaneously improved thetribological properties of PAO6. This discovery contributes to the conversion of BCS into valuables andpromotes the high value utilization of BCS as a lubricating additive.

Expression Profiles of the Insulin-like Growth Factor System Components in Liver Tissue during Embryonic and Postnatal Growth of Erhualian and Yorkshire Reciprocal Cross F₁ Pigs

Pan, Zengxiang,Zhang, Junlei,Zhang, Jinbi,Zhou, Bo,Chen, Jie,Jiang, Zhihua,Liu, Honglin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.7

In Erhualian and Yorkshire reciprocal cross $F_1$ pig populations, we examined the mRNA expression characteristic of liver-derived IGF-1, IGF-1R, IGF-2, IGF-2R and IGFBP-3 during the embryonic and postnatal developmental periods (E50, E70, E90, D1, D20, D70, D120 and D180). Our results demonstrated that the IGF-system genes mRNA levels exhibited an ontogenetic expression pattern, which was potentially associated with the porcine embryonic development, postnatal growth, organogenesis and even the initiation and acceleration of puberty. The expression pattern of IGF-system genes showed variation in the reciprocal cross ($F_1$ YE and EY pigs). This study also involved the expression features of imprinted genes IGF-2 and IGF-2R. The parent-of-origin effect of imprinted genes was reflected by their differential expression between the reciprocal crosses populations. The correlation analysis also indicated that the regulatory network and mechanisms involved in the IGF system were a complex issue that needs to be more fully explored. A better understanding of IGF system components and their interactive mechanisms will enable researchers to gain insights not only into animal organogenesis but also into somatic growth development and even reproduction.

Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

Xian-Ju Huang,Hong-Xiao Zhang,Huili Wang,Kai Xiong,Ling Qin,Honglin Liu 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.4

Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.