사람 위선암에서 cathepsin L의 발현증가

홍원선,홍석일,박인철,손영숙,정훈용,양석균,김해련,민영일 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

cathepsin L은 lysosomal cysteine 단백분해효소로서 기저막(basement membrane)과 세포외기질(extracellular matrix)을 파괴하여 암세포의 침윤과 전이에 중요한 역할을 하는 물질로 알려져 있다. 이러한 cathepsin L에 대한 mRNA 발현도를 5개의 사람 위선암(gastric adenocarcinoma) 세포주와 5명의 위선암 환자에서 채취한 조직에서 방사능으로 표지된 cathepsin L특이 cDNA를 사용한 Northern blot법으로 측정하였다. 위암의 전이병소에서 수립한 세포주인 SNU-5, SNU-16, MKN-45와 Kato Ⅲ에서는 cathepsin L mRNA가 발현되었으나 원발병소에서 수립한 AGS 세포주에서는 mRNA의 발현이 관찰되지 않았다. 5명의 위암 환자에서는 원발병소, 전이가 확인된 임파절 및 암 근처 정상 위점막에서 각각 조직을 채취하여 cathepsin L mRNA의 발현을 측정하였다. 원발병소와 전이병소에서는 모두 cathepsin L mRNA가 발현되었으나 정상 위점막조직에서는 전예에서 mRNA 발현이 관찰되지 않았다. 한편 mRNA의 발현도는 1예에서는 전이병소가 원발병소에 비해 높았으나, 2예에서는 전이병소에서 발현도가 낮았으며, 나머지 2예에서는 원발병소와 전이병소 사이에 차이가 없어, 원발병소와 전이병소 사이에 mRNA의 발현도의 일관성 있는 경향은 관찰되지 않았다. 이상의 결과는 cathepsin L은 위암의 발생과 진행에 있어 암세포의 침윤과 전이를 촉진하는 것 이외에 또 다른 역할을 할 가능성을 시사하고 있다고 사료된다. Cathepsin L, a lysosomal cysteine protease, is known to play an important role in cancer invasion and metastasis by degrading the components of basement membrane and extracellular matrix. The mRNA expression of cathepsin L was determined by Northern blot analysis using a radiolabeled cDNA specific for cathepsin L in five human gastric adenocarcinoma cell lines and five surgical specimens of primary gastric adenocarcinomas, their metastatic lymph nodes and matched adjacent normal mucosae. The mRNA of cathepsin L was expressed in all of the four cell lines established from the metastatic sites, SNU-5, SNU-16, MKN-45 and Kato Ⅲ, while not detected in one cell line established from the primary site, AGS. The mRNA was expressed in all of the five primary and five metastatic cancer specimens tested, while it was not detected in all matched normal mucosae. The intensities of the mRNA expressions, however, did not show the consistent pattern between primary sites and metastatic lymph nodes. These results suggest that cathepsin L may have the other function in addition to facilitation of the invasion and metastasis during the development and progression of stomach cancer.

무용/동작치료가 입원중인 정신분열증 환자의 정신건강 변화에 미치는 영향

김홍자(Hong-Ja Kim),김인숙(In-Suk Kim),김도형(Do-Hyung Kim) 한국콘텐츠학회 2011 한국콘텐츠학회논문지 Vol.11 No.4

본 연구는 무용/동작치료가 입원중인 정신분열증 환자의 정신건강 변화에 미치는 효과를 알아보고자 STAXI, BDI, STAI, PANSS 비교분석 하였다. 대상자는 대전 J병원에서 입원치료를 받고 있는 정신분열증 환자 중 선정기준에 적합한 38명을 무용/동작치료군 18명, 대조군 20명씩 할당하여 분노는 STAXI, 우울은 BDI, 불안은 STAI로, 양성 및 음성증상은 PANSS를 사용하여 비교분석하였다. 상태 분노가 유의하게 감소하였고, 분노 표현 중에서 분노 통제가 유의하게 증가하였다. 우울은 유의하게 감소하였으며, 음성 증후 군 척도에서 유의한 호전을 보였다. 결과적으로 입원한 정신분열병 환자에게 무용/동작치료는 상태 분노를 감소시키고, 분노 통제를 증진시켰고, 우울을 감소와 음성 증상에 긍정적 효과가 나타났다. The purpose of this study was examine the effects of dance/movement therapy on anger and anger expression, depression and anxiety, and positive as well as negative symptoms in in-patient with schizophrenia. Among inpatients with schizophrenia in J Dajeon psychiatric hospital, 38 patients were allocated to either dance and movement therapy (DMT) group (n=18), which included 24 sessions of DMT and medical treatment over 12 weeks, or a control group (n=20), which included only medical treatment. State anger and anger control of DMT group were improved after 12 weeks, and there was a significant difference in anger control after 12 weeks between two groups. Depression of DMT group was improved, and there was significant difference in depression after 12 weeks between DMT and control group. There was a significant difference in negative symptoms after 12 weeks between DMT and control group. These finding demonstrates that DMT has benefit on the reduction of state anger by increasing anger control and depression as well as may improve negative symptoms in in-patient with schizophrenia.

불안과 뇌파와의 관계에 관한 연구

김홍석,김인선 경희대학교 사회체육연구소 1996 體育科學論叢 Vol.- No.9

This study was interested in how different the type pf EEG(Electroencephalograph) is according to the levels of trait and/or state anxiety. Particularly, & respinse of EEG In each anxiety level (high - and low level) was mesaured, and each anxiety level was compared in terms of the ratio of the & wave to the total wave. Two hypotheses were postulated as follows: Ⅰ. & respponse would be different according to trait anxiety level. The ratio of & response to the wave of high anxiety group would be higher than that of low anxiety group in resting situation. Ⅱ. & response would be different according to anxiety level in stressful situation. Junior high school students (48 girls) with the age range of 15 to 16 years were given by questoinnaire to measure the anxiety level. Some items were selected from PIC (the Personality Inventory for Children)and revised for the young students. The upper 10% of the score was selected as high anxiety group (5 girls) and the lower 10% as low anxiety group (5 girls). In both resting - and stressful sityation, EEG was recorded and compaired to both high anxiety - and low anxiety group. The results were that 1) in resting situation, the & response to the total wave on Cz and T3 in high anxiety group was higher than tnat in low anxiety group 2) in stressful situation, & response Cz and T3 in low anxiety group was not any significant change although & response in high anxiety group was increased, and 3)there was not significant difference in & response on O1, known as a part that & response is frequently recorded, in terms of anxiety and experimental condition, These results supported both hypotesis Ⅰ and Ⅱ, and were consistent with the prvious data of Siciliani(1975) and Nowak(1981). The study, as first study to examine the realtionship between anxiety and physiological data, showed that physiological index could be useful in testing of the problems of young students in school.

CIGS 박막의 전착에 관한 연구

이상민,김영호,오미경,홍석인,고항주,이치우,Lee, Sang-Min,Kim, Young-Ho,Oh, Mi-Kyung,Hong, Suk-In,Ko, Hang-Ju,Lee, Chi-Woo 한국전기화학회 2010 한국전기화학회지 Vol.13 No.2

Cu(In, Ga)$Se_2$ (CIGS) 박막은 다원화합물이기 때문에 제조공정이 매우 까다롭다. 진공장치를 사용하는 제조 방법으로 동시증착법, 스퍼터링법 +셀렌화가 있고, 비진공 제조 방법으로 전기화학적인 전착법이 있다. 각 방법에 있어서도 출발 물질의 종류에 따라 다양한 제조 방법이 동원 될 수 있다. 진공증착에 의한 방법은 고품질의 박막을 얻는데 사용 되고 있으나 고가의 진공장비가 사용되므로 대면적화에 따른 제조비용 측면에서 문제가 있다. 이에 비하여, 전착법은 간단하면서도 저가로 대면적화를 이룰 수 있다는 장점 때문에, 많은 관심이 기울여지고 있다. 본 연구에서는 Mo/Glass전극위에 Ga/(In + Ga) = 0.3의 성분비를 만족시키는 CIGS 박막을 전기화학적으로 제조하기 위하여, $Cu^{2+}$, $In^{3+}$, $Ga^{3+}$, $Se^{4+}$ 4성분을 모두 포함하는 전해질 수용액 내에서, 4성분의 이온들이 동시에 환원되는 전위를 조절하여 CIGS 박막을 전착 하였다. SEM을 이용하여 얻어진 CIGS 박막의 전착된 시료의 표면을 관찰하였고, EDS로 그 조성을 분석하였다. 또한, XRD를 이용하여 전착시료의 열처리 전후의 결정성변화를 조사하였다. The chalcopyrite $Cu(In_xGa_{(1-x)})Se_2$ (CIGS) is considered to be one of the effective light-absorbing materials for thin film photovoltaic solar cells. We describe the electrodeposition of CIGS thin films in ambient laboratory conditions, and suggest the electrochemical conditions to prepare stoichiometric CIGS thin films of Ga/(In + Ga) = 0.3. In acidic solutions containing $Cu^{2+}$, $In^{3+}$, $Ga^{3+}$ and $Se^{4+}$ ions, the CIGS films of different Cu/In/Ga/Se chemical compositions were electrodeposited onto Mo/Glass substrate. The structure, morphology and chemical composition of electrodeposited CIGS films were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDS), respectively.

Condylar positioning changes following unilateral sagittal split ramus osteotomy in patients with mandibular prognathism

Kim, Myung-In,Kim, Jun-Hwa,Jung, Seunggon,Park, Hong-Ju,Oh, Hee-Kyun,Ryu, Sun-Youl,Kook, Min-Suk Korean Association of Maxillofacial Plastic and Re 2015 Maxillofacial Plastic Reconstructive Surgery Vol.37 No.-

Background: This study was performed to evaluate three-dimensional positional change of the condyle using three-dimensional computed tomography (3D-CT) following unilateral sagittal split ramus osteotomy (USSRO) in patients with mandibular prognathism. Methods: This study examined two patients exhibiting skeletal class III malocclusion with facial asymmetry who underwent USSRO for a mandibular setback. 3D-CT was performed before surgery, immediately after surgery, and 6 months postoperatively. After creating 3D-CT images by using the In-vivo $5^{TM}$ program, the axial plane, coronal plane, and sagittal plane were configured. Three-dimensional positional changes from each plane to the condyle, axial condylar head axis angle (AHA), axial condylar head position (AHP), frontal condylar head axis angle (FHA), frontal condylar head position (FHP), sagittal condylar head axis angle (SHA), and sagittal condylar head position (SHP) of the two patients were measured before surgery, immediately after surgery, and 6 months postoperatively. Results: In the first patient, medial rotation of the operated condyle in AHA and anterior rotation in SHA were observed. There were no significant changes after surgery in AHP, FHP, and SHP after surgery. In the second patient, medial rotation of the operated condyle in AHA and lateral rotation of the operated condyle in FHA were observed. There were no significant changes in AHP, FHP, and SHP postoperatively. This indicates that in USSRO, postoperative movement of the condylar head is insignificant; however, medial rotation of the condylar head is possible. Although three-dimensional changes were observed, these were not clinically significant. Conclusions: The results of this study suggest that although three-dimensional changes in condylar head position are observed in patients post SSRO, there are no significant changes that would clinically affect the patient.

Compression injury of the circular stapler for gastrointestinal end-to-end anastomosis: preliminary in-vitro study

손경모,Myeong Sook Kwon,Hong-min Ahn,In Young Lee,김건호,남경원,JoonWon Lee,Chang-Suk Kong 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.2

Purpose: This preliminary in-vitro study was designed to evaluate the risk factors of compression injury from use of a circular stapler for end-to-end anastomosis. Methods: Transparent collagen plates were prepared in dry and wet conditions. Physical properties of collagen plates and porcine colon tissue were examined using a rheometer. Adjustable and fixed-type circular staplers were applied on the collagen plates and the gap distance and compressive pressure were measured during anvil approximation. Tissue injury was evaluated using a compression injury scale. Compression properties were accessed to optimal or overcompression based on gap distance. Results: Unacceptable injuries were rarely observed on the dry collagens, regardless of compression device. In the adjustable compression, the compressibility ratio was similar between dry and wet collagen. Overcompression and unacceptable injury increased on the wet collagens. In the fixed compression, the compressibility ratio increased significantly and unacceptable injuries were observed in more than 50% of wet collagens. Peak pressure was significantly higher in the fixed-compression types than those of adjustable type. On bivariate correlation analysis, fixed-compression type and wet collagens were respectively associated with overcompression. On multivariate analysis, edematous collagen condition was the most important risk factor and proximal anvil side, fixed compression type, and overcompression were also independent risk factors for unacceptable compression injury. Conclusion: In the edematous tissue condition, unintentional overcompression could be increased and result in tissue injury on the compression line of the circular stapler.

가정간호대상자와 입원간호대상자의 간호만족도 및 간호사이미지 비교

용진선,한성숙,유인자,홍현자,Yong, Jin-Sun,Han, Sung-Suk,Yoo, In-Ja,Hong, Hyun-Ja 한국가정간호학회 2002 가정간호학회지 Vol.9 No.1

The purpose of the study was to compare both the nursing care satisfaction and the image of nurses as experienced by home health care clients and hospitalized clients. For the descriptive survey study. data were collected from 69 home heath care clients and 342 in-patients in a university hospital. The tools used for the study were modified by Quality Patient Care Scale(Wandelt & Ager. 1974) and Image of Nurses (송인자, 1993). The data were analyzed using Pearson Correlation. Scheffe test. factor analysis. t-test. and ANOVA. The major findings were as follows: Regarding nursing care satisfaction, the mean score of total nursing care satisfaction in home health care clients was 3.28 out of 4.0. Among five domains, the domain with the highest score was the psychosocial domain, followed by the general. the professional. the physical. and the communication domain. The level of nursing care satisfaction was not significantly different according to demographic variables except for age: the age group of 41-60 showed the highest score (p<0.05). The mean score of total nursing care satisfaction in hospitalized clients was 2.95 out of 4.0. Among five domains, the domain with the highest score was the psychosocial domain, followed by the physical and the communication. the professional. and the general domain. The level of nursing care satisfaction was not significantly different according to demographic variables except age: the higher the age the higher the score (p<0.05). The levels of nursing care satisfaction in all five domains were significantly higher in home health care clients than in hospitalized clients(p=0.0005). Regarding image of nurses, the mean score of total image of nurses in home health care clients was 3.32 out of 4.0. Among four domains, the domain with the highest score was the sincerity domain, followed by the kindness. the spirit. and the knowledge and skill domain. The level of image of nurses was not significantly different according to demographic variables. The mean score of total image of nurses in hospitalized clients was 3.05 out of 4.0. Among four domains. the domain with the highest score was the sincerity domain, followed by the kindness, the knowledge and skill, and the spirit domain. The level of image of nurses was not significantly different according to demographic variables. The levels of image of nurses in all four domains were significantly higher in home health care clients than in hospitalized clients (p=0.001). Both the levels of nursing care satisfaction and image of nurses, part of an evaluation for quality of nursing care were significantly higher in home health care clients than in hospitalized clients. In light of the findings, we could consider that home health care nurses provided client-centered comprehensive nursing care. However, nurses need to have methods that more promote the social recognition of the image of nurses and nursing care services as well as professional knowledge and skills.

자돈에 있어 게르마늄 용질액의 급여가 성장 및 혈액학적 변화에 미치는 영향

홍종욱,권오석,민병준,조진호,진영걸,손경승,강종옥,김인호 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

본 연구의 목적은 이산화게르마늄을 함유한 게르마늄 용질액을 자돈에 급여하였을 때 성장 및 혈액학적 변화에 미치는 영향을 조사하기 위하여 실시하였다. 3원 교잡종 자돈 60두 (평균체중 11.22±0.10㎏)를 공시하였다. 시험설계는 옥수수-대두박 위주의 대조구(CON)와, 대조구 사료내 게르마늄 용질액을 0.5 ppm 첨가한 구(GC0.5) 및 대조구 사료내 게르마늄 용질액을 1.0 ppm 첨가한 구(GC1.0)로 3개 처리를 하였다. 0-10일간 사양시험기간동안, 일당증체량에 있어서 GC0.5 처리구가 약간 높게 평가되었다(quadratic effect, P<0.02). 그러나 전체 시험기간동안, 일당증체량, 일당사료섭취량 및 사료효율에 있어서 처리구간에 유의적인 차이는 보이지 않았다. 영양소 소화율에 있어서는 GC0.5 처리구가 조회분(linear effect, P<0.01; quadratic effect, P<0.04), 칼슘(linear effect, P<0.01; quadratic effect, P<0.01), 인 (linear effect, P<0.02; quadratic effect, P<0.04) 소화율이 유의적으로 높게 평가되었다. 혈액내 neutrophil 농도에 있어서는 게르마늄 용질액의 첨가 수준이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다(quadratic effect, P<0.02). 결론적으로, 자돈 사료내 게르마늄 용질액의 급여가 성장 및 혈액학적 수치에는 영향을 미치지 못하는 것으로 사료된다. This study was conducted to investigate the effects of dietary germanium colloid on the growth performance and hematological values in nursery pigs. Sixty pigs (11.22(0.10 ㎏ average initial body weight) were used in a 20 d growth assay. Dietary treatments included 1) CON (basal diet), 2) GC0.5 (basal diet+0.5 ppm germanium colloid) and 3) GC1.0 (basal diet+1.0 ppm germanium colloid). For d 0 to 10, pigs fed GC0.5 diet grew faster than pigs fed CON and GC1.0 diets (Quadratic effect, P<0.02). However, through the entire experimental period, no statistical differences were found for aver-age daily gain, average daily feed intake and gain/feed. Apparent digestibilities of crude ash (Linear effect, P<0.01; Quadratic effect, P<0.04), calcium (Linear effect, P<0.01; Quadratic effect, P<0.01) and phosphorus (Linear effect, P<0.02; Quadratic effect, P<0.04) in pigs fed GC0.5 diet were greater than for pigs fed CON and GC1.0. Neutrophil concentration in blood increased as the concentration of germanium colloid in the diets was increased with significant difference (Quadratic effect, P<0.02). In conclusion, growth performance and hematological values were not influenced by dietary germanium colloid.

wmlTo.NET 모바일 웹 페이지 변환 시스템 설계 및 구현

홍인숙,김윤중,김승연 한밭대학교 정보통신전문대학원 2004 정보통신전문대학원 논문집 Vol.2 No.1

본 논문에서는 WML 페이지를 NET 모바일 웹 페이지로 변환하는 시스템을 구현하였다. 본 변환시스템 을 어휘분석기 모듈과 구문 지향 번역기 모듈로 구성되어 있는데 각각의 모듈은 어휘분석기 생성기(LEX)와 파서 생성기(YACC)를 사용하여 구현하였다. 어휘분석기 모듈은 WML페이지를 입력받아 각 태그들을 분석하고 토큰을 출력한다. 구문 지향 번역기 모듈은 어휘분석기 모듈에서 출력된 토큰들을 입력받아 문법의 유효성을 체크하고 parsing이 완료되면 대응되는 .NET의 모바일 컨트롤로 변환하여 최종적인 .NET모바일 웹 페이지를 생성한다. LEX와 YACC의 사용으로 향후 태그를 확장하거나 WML의 버전이 업그레이드 될 때에도 변경된 부분에 해당하는 문법만 다시 설계함으로써 유연하게 대처할 수 있는 장점을 가지고 있다. 구현된 변환 시스템을 이용하여 서비스를 제공할 수 있는 모바일 컨텐츠 개발의 생산성을 향상시키고 개발기간과 비용을 단축해 신속한 무선 인터넷을 제공하는데 그 목적이 있다. In this paper, we describe a design and implementation of a Conversion System for NET Mobile Web Page in Wireless Markup Language(WML) Page. The Conversion System consists of the LA(Lexical Analyzer) and SDT.(Syntax-directed Translation) module. LA module analyzes every tags in WML page and makes tokens as output. SDT module checks grammar for every tags with tag produced by LA module. After parsing process is completed in SDT module, SDT module creates mobile web page with token recognized by LA. Each LA and SDT implemented in this system uses LEX and YACC. Even though the tags in WML will extended or WML DTD will upgraded, this approach has the advantage of flexibility because it's only needed to redesign the grammar for mended information of tags.

中山間地(標高 250m)에서 참깨 播種適期와 晩播限界期에 關한 硏究

孫錫龍,崔仁植,趙鎭泰,權圭七,金仁培,李濟弘 충북대학교 농업과학기술연구소 1986 農業科學硏究 Vol.4 No.-

In order to determinate the optimum plating time and its limitation, an eary harvest and high yeld sesame varety-Poong Nyun Ggae-was cultivated on the semi-mountainous area of 250 meter above sea level. The variety was cultivated with a single and double cropping system. The single cropping plots were covered with transparent polyethylene film on 1st , 11th and 21th in May and the double cropping plots were covered with PE film on 5th, 15th, 25th in June respectively and on 5th July. The results obtained are as follows; Yield of the plot planted on 1st May was 59kg/10a. In compaorisn with this, yields were increased 16% and 25% in plots planted on 11th and 21th May, respectively. The optimum planting time was considered near 20th May for the single cropping system and it should be considered before 15th June for the double cropping system in the semimountainous area.