Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

2급 와동 수복 시 한국 치과 의사들의 복합레진 사용 실태 연구

신동호,박세은,양인석,장주혜,이인복,조병훈,손호현 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

본 연구는 2008년 1월 현재 한국 치과의사들의 복합레진 사용 실태에 관한 설문 조사로서, 2급 와동을 수복하는 증례에서 치과의사의 면허 년도, 교육 배경 및 진료환경 등에 따라 수복재료의 선택, 복합레진의 사용여부 및 사용방법, 문제점 등을 비교하였다. 한국 치과의사들을 대상으로 2급 와동 수복에 관한 17 문항의 설문지를 제작하였다. 이 설문지는 시술자 정보 2급 와동의 수복으로 크게 2부분으로 나누어진다. 이 설문지는 대한치과의사협회를 통해 12.193 명의 치과의사들에게 E-메일로 발송되었다. 이 중 2.612개의 메일이 수신 확인되었고 840 개의 설문지가 작성되어 회신되었다. 수신 확인된 메일 수에 대한 회신된 메일 수의 비율 (회신율)은 32.2%이었고, 이 자료는 SPSS프로그램에서 카이제곱 분석을 이용하여 교차 분석하였다. 답변자의 비율은 면허년도를 기준으로 1998 ~ 2007년에 면허를 취득한 그룹 (한국 전체 치과의사의 33 3%, 추정 년령 26-35세)이 60.3%로 1997년 이전에 면허 취득한 그룹 (한국 전체 치과의사의 66.7%.추정 년령 36세 이상)의 39.7%보다 높은 것으로 나타났다. 또한 이들이 근무하는 병원은 개인의원 (77%)이 가장 많았고 남자 치과의사(79%)가 많았다. 복합레진 수복에 대한 지식은 학생 때 수업이나 학회, 세미나를 통하여 (83.4%) 얻은 것으로 나타났다. 2급 와동 수복 시 재료 선호도를 살펴보면 금인레이가 65.7%를 차지하고 있고 복합레진 직접 수복은 12.1%로 낮게 나타났다. 2급 와동의 복합레진 직접 수복 시 시술 방법에 있어서는 러버댐을 사용하지 않거나 잘 사용하지 않는 그룹의 비율(74.4%)이 더 많았고. 격벽법으로는 mylar strip (53.4%)이나 metal matrix (33.8%), Palodent svstem (6.5%)를 사용하였다. 충전은 적층법 (99.6%)을 사용하는 것으로 나타났으며, 시술 시 인접면 형성을 가장 어려워하였다.(57.2%). 2-step 접착시스템 (76%)이 3-step 접착시스템 (16%)보다 많이 사용되고 있었다. 사용하는 레진 제품으로는 Z250(20%)이 가장 많이 사용되고 있었다. The purpose of this study was to assess the current materials, methods and difficulties according to the year of licence and educational background of Korean dentists in Class Ⅱ direct composite resin restorations. Total 17 questions were included in the questionnaire. Questions were broadly divided into two parts first, operator's information and second the materials and methods used in Class Ⅱ posterior composite restoration. The questionnaire was sent to dentists enrolled in Korean Dental Association via e-mail. Total 12,193 e-mails were distributed to dentists. 2,612 e-mails were opened and 840 mails (32.2%) were received from respondents. The data was statically analyzed by chi-square test using SPSS(v 120.1 SPSS Inc. Chicago. IL USA). Male dentists among respondents was 79% 60.3% of the respondents acquired their licences recently (1998-2007) and 77% practiced in private offices 83.4% have acquired their knowledge through school lectures conferences and seminars. For the Class Ⅱ restorations gold inlays were preferred by 65.7% of respondents while direct composite resin restorations were used by 12.1% amalgam users were only 4.4% of respondents. For the restorative technique 74.4% of respondents didn't use rubber dam as needed. For the matrix. mylar strip (53.4%), metal matrix (33.8%) and Palodent system (6.5%) were used 99.6% of respondents restored the Class Ⅱ cavity by incremental layering. Obtaining of the tight interproximal contact was considered as the most difficult procedure (57.2%) followed by field isolation (21%). Among various bonding systems, 22. 6% of respondents preferred SE Bond and 20.2% used Single Bond. Z-250 was used most frequently among a variety of composite resins.

18S-srRNA 분석에 의한 Acantbamoeba 한국 분리주들의 분류 검토

신호준,지영진,조명수,김형일,박연희,임경일 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.2

Acanthamoeba spp. spread in soil, ponds, air and swimming pool, cause a granulomatous amoebic meningitis and amoebic keratitis in human and experimental animals. Their classification had depended upon morphological characteristics and physiological conditions such as thermophilic status and generation times. However, because amoeba species showed the morphological and physiological diversity in isolates, many attempts have been made on identification of Acanthamoeba spp. Restriction fragments length polymorphism (RFLP) analysis of IBS-small subunit ribosomal RNA (srRNA) was an useful tool for classification among morphologically and genetically closely-related species. In this study, 18S-srDNAs of amoebae were amplified by PCR with primer encoding 18S-srRNA gene and digested with restriction endonudeases, and RFLP analysis was applied on classification of Acanthamoeba Korean isolates (YM-4, YM-5 and YM-7), comparing with reference amoebae, A. culbertsoni, A. polyphaga and A. royreba. Trophozoites of six Acanthamoeba spp. had typical acanthopoda, but did not show any morphological differences. Cyst of Acanthamoeba sp. YM-7 was similar to that of A. polyphaga which was designated as group Ⅱ Acanthamoeba. Morphologically other amoebae belonged to group Ⅲ Acanthamoeba. Digestion of 18S-srDNA with six enzymes, Dde Ⅰ, Hae Ⅲ, Hind Ⅲ, EcoR Ⅰ, Rsa Ⅰ and Sph Ⅰ, resulted in various DNA fragments. Using the method of Nei and U (1979) for RFLP analysis, genetic divergence was not observed between A culbertsoni and Acanthamoeba sp. YM-4. Between A. culbertsoni and Acanthamoeba sp. YM-5, genetic distance was 0.070, 0.364 between Acanthamoeba sp. YM-4 and A. polyphaga, and 0.277 between Acanthamoeba sp. YM-7 and A. polyphaga. In comparison with A. culbertsoni and A. polyphaga, Acanthamoeba sp. YM-7 showed genetic distance of 0.330 and 0.154, respectively. Thus, Acanthamoeba sp. YM-7 is a similar species to A. polyphaga. Acanthamoeba sp. YM-5 is a different species from A. polyphaga, but closely-related to A. culbertsoni. Acanthamoeba sp. YM-4 can be classified as a subspecies or a strain of A. culbertsoni.

韓國産 十字花科 植物에 關한 本草學的 硏究

趙南俊,金台睍,宋昊埈,辛民敎 한국전통의학연구소 1999 한국전통의학지 Vol.9 No.1

For the purpose of developing Korean Herbalogy with the plants of Cruciferae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigative and the results obtained were as follows: 1. There were totaled to 23 genera and 74 species in Korea and among them medicinal plants are 13 genera, 29 species, some 39% in total but, the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging, to the Cruciferae family were classified as Herba 8,Semen 7,Radix 4,Foilum,Flos 3,Rhizoma 3,Fructus 1. Thus it was noticed that Herba was the main kind. 3. According to sum of 74 species in Cruciferae family, they were classified into Arabis genera 16, Brassica, Lepidium genera 5 each. Thus it was noticed that Arabis genera was the main kind, some 23% in total. 4. According to the number of species of the origin plants about each chinese materia medicals, they were classified into SEMEN LEPIDII 10,SEMEN SINAPIS 2. Thus the origin plants was not complexed reletively. 5. According to nature and flavour of medicinal plants, they were classified into warmth, heat; 4 each, cold, cool; 7 each, balance 14. Thus it was noticed that balance is the main in nature and flavour of medicinal plants. 6. According to the Properties and Principal curative action, they were classified into Drugs for remove toxic heat with febrifugal and detoxicant drugs, to removing evil heat and resolve dampness ,Drugs for reducing intense internal Heat Digestives and Evacuants 6 each, with promote or activate blood circulation by removing blood stasis ,to clear up heat and resolve phlegm, to remove toxic heat with febrifugal and detoxicant drugs such as 6 each, to regulate the flow of vital energy, to replenish vital essence with drugs 5 each, to dispel pathogenic factors from the exterior of the body with sudorifics pungent in flavor and warming in property, to stop diseases with drugs 3 each. Thus it wasnoticed that to promote or activite blood circuration by removing blood stasis drugs was the main kind. 7. Comparing to whole medicinal plants 89 kinds, toxic durgs include minor toxin were 8 kinds, 9% of the whole.Thus toxic durgs were rare. From this result, it was revealed that the plants for medical purpose in Liliaceae was 76% of the whole, in which Bulbus was mostly abundunt and toxic plants was so rare that it will be used for clinical treatments more easily. It is considered that many experiements and clinical approaches must be continued to use Liliaceae plants widely.

祖稅의 超過負擔에 관한 考察

曺新鎬 강남대학교 사회과학연구소 1999 사회과학논총 Vol.8 No.-

성차와 리더십 : 문헌적 고찰 A Literature Review

조경순,이신자,김호원 숙명여자대학교 아시아여성연구소 2004 아시아여성연구 Vol.43 No.1

최근 여성 리더십에 대한 기업 조직과 학문 분야의 관심이 커지고 있지만 아직 여성 리더십과 관련된 다양한 개념들은 혼용되어 사용되고 있다. 여성 리더와 여성 리더십 연구의 체계적 접근을 위해 생물학적 성과 젠더의 측면에 따른 남녀의 차이와 남녀의 리더십 차이, 그리고 남녀의 리더십 유효성 차이를 경영학 분야에서 연구된 실증연구를 토대로 문헌 고찰을 하였다. 그 결과 성차가 존재하는 가에 대한 물음에 성차는 남녀의 생물학적 차이를 제외하면 성역할 사회화를 통해 만들어지는 것으로 보는 경향이 강한 것으로 나타났다. 리더십의 성차는 리더십 특성과 행위에 차이를 보이는 경향으로, 리더십 유효성에 대한 남녀 차이는 남성이 리더십을 더 발휘한다고 평가하는 경향이 많은 것으로 나타났다. 그러나 리더십과 리더십 유효성의 남녀 차이에 대한 연구는 일관된 결과를 보여주지 못하였으며, 조절변수에 따라 그 차이가 달라질 수 있다고 보고되고 있다. 추가적으로 남녀의 리더십과 리더십 유효성의 차이는 생물학적 성에 의한 차이보다 젠더차에 의한 것이 크며, 이는 성을 남성성과 여성성 및 양성성으로 구분하여 유효성에 대해 남녀 차이를 살펴보는 등의 다양한 연구의 시도들이 필요하다. The literature of leadership research has emphasized the importance of gender issue not only from an academic perspective but also from practical viewpoint. However, the literature seems unclear about many gender-related leadership conceptualizations. This study reviews theoretical and empirical research in organizational behavior,and aims to draw clear a view of the current state of research regarding gender issues in leadership research. Our understanding of the conceptual difference of biological sex and sociological gender of women and men led us to focus on sex- and gender-related differences in leadership, especially in leadership effectiveness. Our review indicates that most of the differences displayed by women- and men-leaders are attributable to their gender- role socialization, rather than their biological sex. It is also found that men-leadership is more effective in general, though the relationship in not very consistent and could be moderated by various factors such as nature of task, composition of the work group, and support from family. Our findings imply that gender differences in leadership are primarily based on socially constructed reality, which suggests future studies on gender issues in leadership to consider masculinity, femininity, and androgyny as major factors that have influence on leadership effectiveness differences.

무릎관절 추적훈련이 편마비 환자의 보행에 미치는 영향

신화경,염호준,조상현,장성호 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.3

우울증 선별 검사 도구로서 Beck Depression Inventory(BDI)의 타당성 : Detection of depression in primary care

신호철,김철환,박용우,조비룡,송상욱,윤영호,오상우 大韓家庭醫學會 2000 Korean Journal of Family Medicine Vol.21 No.11

中央銀行改革과 인플레이션目標 管理政策

曺新鎬 강남대학교 사회과학연구소 1998 사회과학논총 Vol.6 No.-