Limulus Amoebocyte Lysate법에 의한 원유와 살균유의 세균수 측정

姜國熙,王智怨,朴興洙,李圭鍾,金敬民 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2

LAL method for the determination of Gram-negative bacterial Lipopolysaccharides(LPS) in milk is proposed and its value for bacteriological quality control is investigated. The relationship between the LPS contents and total colony count(cfu/ml), which covers the Gram-negative bacteria, was determined in raw milk samples. The coefficient of correlation between Gram-negative bacterial counts(cfu/ml) and LPS contents was r^2 =0.969. And, the correlation coefficient between total bacterial counts(cfu/ml) and LPS contents was r^2 =0. 914. It was also investigated how much the LPS contents were in UHT milks from l0 different dairy industrial companies in Korea. It varied from 92 ng LPS/ml to 938 ng LPS /ml.

고등학교 지구과학 실험·실습 요목 비교 분석

정원우,이윤종,기우항,김영호,양승영,강용희,안병호,임성규,윤일희,김중욱,윤성효,강동진 경북대학교 과학교육연구소 1997 科學敎育硏究誌 Vol.21 No.-

The purpose of this study is to investigate the status of the experimental and practical education in high school earth. The present status and reasonable management of the experimental and practical education in high school earth science have been grasped from the questionaires. To do this, eighty eight earth science teachers in Korea are administered questionaires. The frequency of the experimental and practical items in the seven kind of earth science text books were investigated. The problems and the reasonable management for experimental and practical education were proposed in this paper.

유전자 알고리즘과 신경회로망을 이용한 고속 확관기의 확관속도 최적화

정원지,김재량,김진한,홍대선,강흥식,김동성 한국공작기계학회 2005 한국생산제조학회지 Vol.14 No.2

This paper presents the optimization of expanding velocity for tube expanding process in the manufacturing of a heat exchanger. In specific, the expanding velocity has a great influence on the performance of a heat exchanger because it is a key variable determining the quantity of tube expanding at assembly stage as well as a key parameter determining overall production rate. The simulation showed that the genetic algorithm used in this paper resulted in the optimal tube expanding velocity by performing the following series of iteration; the generation of arbitrary population for tube expanding parameters, consequently the generation of tube expanding velocities, the evaluation of tube expanding quantity using the pre-trained data of plastic deformation by means of a neural network, and finally the generation of next population using a penalty function and a Roulette wheel method.

보릿짚의 수분함량 및 수확시기가 소각시 대기오염물질 발생에 미치는 영향

고지연,강항원,이재생,김춘송,박성태,김복진 한국환경농학회 2004 한국환경농학회지 Vol.23 No.2

영남지방 벼-보리 이모작답에서 가장 널리 시행되고 있는 보릿짚 처리방법인 소각이 대기환경에 미치는 영향을 소각시 발생하는 온난화가스, 대기오염가스 및 분진발생량을 중심으로 살펴보았다. 보릿짚 소각시 온난화가스 발생량은 CO₂376.8, CH₄ 1.56, N₂O 0.05 kg/10a 로서 CO₂의 발생량이 가장 많았으며, 대기 오염가스 중에는 유기물의 불완전 연소시 배출되는 CO발생량이 28.8 kg/10a로서 가장 많았다. 그 외 SO₂ H₂S, NH₃, NO등의 질소 및 황 함유 유해가스도 소량 발생되었고 발생된 PM 10 분진의 대부분은 인체에 미치는 영향이 PM 10보다 큰 PM 2.5로 이루어져 있었다. 또한 수확시기가 빠르거나 수분함량이 높은 보릿짚일수록 온난화가스 및 대기오염 가스의 발생량이 증가하고 소각 연기내 PM 10 분진중 PM 2.5의 미세분진 비중이 높았다. 수확적기로 알려진 출수 40일 이후의 보릿짚은 출수 후 40일된 보릿짚 소각시 발생하는 대기오염가스 발생량과 큰 차이가 없었다. This study was carried out to determine impacts of burning of barley straw produced from rice-barley double cropping paddy field on air quality by investigating emissions of greenhouse gases (CO₂, CH₄ and N₂O), air pollution gases (CO, SO₂, H₂S, NH₃ and NO) and particulate matters (PM 10 and PM 2.5). When the barley straw at a rate of 4.5 t/ha was burned at open status, the emitted GHGs amounts were CO₂ 376.8 kg/10a, CH₄ 1.56 and N₂O 0.06. The amount of CO emission was the largest among air pollution gases. These results showed that the range of 45~55% of total C in barley straw was emitted as CO₂-C, followed by CO-C (6.4~5.9%) and CH₄-C (0.5~0.7%). As far as moisture content in barley straw is concerned, the higher moisture content that the barley straw contains, the larger amount of air pollution gases and the higher portion of PM 2.5 in PM 10 were emitted when it burned. In case of harvesting time of barley straw, emission amounts of greenhouse, air pollution gases and PM 2.5 portion in PM 10 had tendency to increase when earlier harvested barley straw was burned.

소수계 유역 인공습지에서 식생 밀도 차이에 따른 영양염류 제거효율

고지연,강항원,이재생,김춘송,Sakadevan,K.,Bavor, H. J. 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

호주 시드니 인근 도·농 복합 소도시로부터 발생하는 비점오염원을 처리하기 위하여 설치된 Plumpton park와 Woodcroft park 인공습지의 처리효율을 살펴본 결과, 수생식물의 밀도가 증가하고 식생이 안정된 Plumpton park 인공습지에서 T-P의 26.2%, T-N의 38.3%, 식생분포가 안정적이지 않은 Woodcroft park에서 14.0%와 20.2%가 각각 제거됨으로서 식물체 근권 미생물에 의한 T-P 및 T-N의 제거 효율이 상당히 큼을 알 수 있었다. 또한 T-N와 T-P에 비하여 NH₄-N, NO₃-N, PO₄^(-3)의 제거율이 높았던 것은 무기태 형태의 영양염류가 식물 및 미생물에 더 쉽게 이용되었기 때문으로 생각된다. 이상의 결과로 볼 때 인공습지에서 식물체와 근권 주위 미생물에 의한 염류제거는 인공습지가 설치한지 오래되었을 때도 효과적이었고, 또한 인공습지는 무기태 영양염류의 제거효율이 더욱 높았으므로 무기화학비료 등의 용탈이 일어나기 쉬운 농경지 비점오염원으로부터 발생하는 수질오염의 개선에 이용할 수 있는 경제적이고도 효율적인 system으로 고려되었다. This study was conducted to evaluate effects of 2 constructed wetlands with different vegetation states (Plumpton park wetland and Woodcroft park wetland) for reducing non-point source pollution from small watershed consisted of residential and agricultural area in suburban district of Sydney, Australia. The total nitrogen and phosphate removal efficiency of Plumpton park constructed wetland, composed of stable and dense vegetation, were 38.3% and 26.2% and Woodcroft park constructed wetland having still poor vegetation due to the short time to settle down transplanted plants after construction, showed relatively low removal efficiency of 20.2% and 14.0%. The removal efficiency of inorganic nutrients such as NH₄-N, NO₃-N, PO₄^(-3) were higher than total nitrogen and phosphate because plants and microorganisms in rhizosphere of constructed wetlands took up inorganic nutrients shortly. According to the type of wetland inflow, the nutrients removal efficiency of storm water flow was lower than base flow.

현행 중학교 과학 실험·실습교육에 대한 교사들의 견해와 개선방안

이윤종,기우항,김영호,정원우,양승영,강용희,안병호,임성규,윤일희,김중욱,강동진 경북대학교 과학교육연구소 1996 科學敎育硏究誌 Vol.20 No.-

The status of facilities, management of the experiment, practices, and Science education teaching method in middle school have been investigated. The present status and reasonable management of middle school science education have been grasped from the questionaires. The results are as follows : The reasonable management for expeiments and practices of science education were scanty in the middle school around the urban and rural schools owing to the shortage of facilities and equipments, crowded class, excessive classes, work for teachers, excessive contents of present textbook and insufficience of administrative support etc. The current teaching method of middle school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of teaching method. Desirable directions for the improvement of present status of middle school science education were proposed in this paper.

갈륨액체금속 이온원과 인듐액체금속 이온원의 빔 특성에 대한 연구

현정우,임연찬,정강원,정원희,박철우,이종향,강승언 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

본 연구에서는 인듐 액체금속이온원을 제작하여 빔 특성에 대해 연구를 하였으며, 기존에 연구를 하였던 갈륨 액체금속이온원의 빔특성과 비교 분석 하였다. 빔특성 분석을 위해 빔 안정도, 전류-전압특성곡선, 에너지 퍼짐을 측정하였다. 액체금속이온원에 사용되는 액체금속 저장소 및 바늘전극(tip)은 500μm의 직경을 갖는 텅스텐을 사용하였으며, 국내에서 제작된 제품을 사용하였다. 액체금속 저장소의 구조는 이전에 구상하여 연구가 이루어진 6개의 pre-etching된 텅스텐와이어(wire)가 묶여진 형태를 사용하였다.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

Antitumor Activity and Acquired Resistance Mechanism of Dovitinib (TKI258) in <i>RET</i>-Rearranged Lung Adenocarcinoma

Kang, Chan Woo,Jang, Kang Won,Sohn, Jinyoung,Kim, Sung-Moo,Pyo, Kyoung-Ho,Kim, Hwan,Yun, Mi Ran,Kang, Han Na,Kim, Hye Ryun,Lim, Sun Min,Moon, Yong Wha,Paik, Soonmyung,Kim, Dae Joon,Kim, Joo Hang,Cho, American Association for Cancer Research 2015 Molecular Cancer Therapeutics Vol.14 No.10

<P><I>RET</I> rearrangement is a newly identified oncogenic mutation in lung adenocarcinoma (LADC). Activity of dovitinib (TKI258), a potent inhibitor of FGFR, VEGFR, and PDGFR, in <I>RET</I>-rearranged LADC has not been reported. The aims of the study are to explore antitumor effects and mechanisms of acquired resistance of dovitinib in <I>RET</I>-rearranged LADC. Using structural modeling and <I>in vitro</I> analysis, we demonstrated that dovitinib induced cell-cycle arrest at G<SUB>0</SUB>–G<SUB>1</SUB> phase and apoptosis by selective inhibition of RET kinase activity and ERK1/2 signaling in <I>RET</I>-rearranged LC-2/ad cells. Strong antitumor effect of dovitinib was observed in an LC-2/ad tumor xenograft model. To identify the acquired resistance mechanisms to dovitinib, LC-2/ad cells were exposed to increasing concentrations of dovitinib to generate LC-2/ad DR cells. Gene-set enrichment analysis of gene expression and phosphor-kinase revealed that Src, a central gene in focal adhesion, was activated in LC-2/ad DR cells. Saracatinib, an src kinase inhibitor, suppressed ERK1/2 phosphorylation and growth of LC-2/ad DR cells. Taken together, these findings suggest that dovitinib can be a potential therapeutic option for <I>RET</I>-rearranged LADC, in which acquired resistance to dovitinib can be overcome by targeting Src. <I>Mol Cancer Ther; 14(10); 2238–48. ©2015 AACR</I>.</P>

No-till Farming System: Research Direction and Outlook in Korea

Hang-Won Kang,Min-Tae Kim,Kwang Seop Kim,Weon-Tai Jeon,Jin-Hee Ryu,Ki-Yeong Seong 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.3

No-till farming system has been extensively studied all over the world as the effective method for maintaining the soil fertility. The general advantages of this system have been well known for reducing the labor, fuel, machinery, and irrigation cost as well as for increasing the soil quality through soil aggregation, water infiltration, microbial population and etc. Recently, it becomes more popular with the increase of interest on sustainable agriculture, especially because of its higher carbon sequestration potential compared to conventional tillage. Crop residue management should be essentially included to look forward to achieving the positive effect on reduction of greenhouse gas. Nonetheless, there are also negative opinions on effect of no-till farming system. For example, some researchers reported that soil physical properties were not improved by no-till under certain soil and climatic conditions. This means no-till farming systems were strongly affected by the soil characters and climatic conditions. Therefore, the researches to meet the specific-regional characters are greatly needed in order for no-till farming system to successfully settle in Korea. The objective of the review article is to present the future direction and perspective on no-till farming system in Korea. For this purpose, we summarized the results of domestic and foreign researches about no-till farming system until now. Specifically, the chapter on foreign research consisted of four parts: positive and negative effects, the effect in paddy soil, and latest research direction (2012-2013) of no-till farming systems. Whereas, review for domestic researches was divided into two main parts: paddy and upland soils. In the final chapter, the priorities for the optimum conservation tillage in Korea were discussed and proposed through the previous researches.