Sterols and Triterpenoids from Codonopsis lanceolata

Hang Suk Yang,Sung Sook Choi,Byung Hoon Han,Sam Sik Kang,Won Sik Woo 대한약학회 1975 약학회지 Vol.19 No.3

The roots of Codonopsis lanceolata contained alpha-spinasterol, delta7-stigmastenol, oleanolic acid, echinocystic acid, and an unidentified triterpene acid, MP 249o.

Plenary Session 1 : PS-1-1 ; Effects of Lactobacillus paracasei on kupffer cell polarization in non-alcoholic steatohepatitis model

( Won Sohn ),( Dae Won Jun ),( Kang Nyeong Lee ),( Hang Lak Lee ),( Tae Yeob Kim ),( Joo Hyun Sohn ),( Oh Young Lee ),( Byung Chul Yoon ),( Ho Soon Choi ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Recently, gut microbiota has been received attention in pathogenesis of non-alcoholic fatty liver disease (NAFLD). The protective effects of Lactobacillus paracasei on NAFLD and possible mechanism were investigated. Methods: Forty male C57BL/6 mice were randomized into 4 groups for 12 weeks: control, NASH model (high fat+10% fructose diet), Lactobacillus paracasei and Lactobacillus plantarum groups. Hepatic fat deposition, inflammation, serum ALT, AST, and bilirubin were tested. Kupffer cell polarization was evaluated by flow cytometry using M1 and M2 marker. Intestinal permeability was measured by urinary Cr-EDTA amount. Results: Mean body weight of L. paracasei and L. plantarum group were lower than NASH model (38 g vs. 43g, p<0.05). Compared with NASH model mice, serum ALT and AST were significantly decreased in both L. paracasei group and L. plantarum group (ALT: 135.5 U/L vs. 92.9 and 53.7U/L, p<0.05). Intestinal permeability was decreased in both L. paracasei and L. planatrum group compared to NASH model (p<0.05).While hepatic fat deposition and lobular inflammation was significantly decreased in both L. paracasei and L. plantarum groups. Kupffer cell infiltration was significantly low in only L. paracasei group (p<0.05). M2 macrophage population increased in L. paracasei compared to NASH group (62.1% vs 50.2%, p<0.05). But Kupffer cell polarization of L. plantarum group did not showed differences compare to NASH group. Conclusions: Probiotics attenuate hepatic fat deposition and decrease ALT and AST levels in NASH model. L. paracasei, but not L. plantarum prevented steatohepatitis via modulation of Kupffer cell polarization.

Plenary Session 1 : PS-1-1 ; Effects of Lactobacillus paracasei on kupffer cell polarization in non-alcoholic steatohepatitis model

( Won Sohn ),( Dae Won Jun ),( Kang Nyeong Lee ),( Hang Lak Lee ),( Tae Yeob Kim ),( Joo Hyun Sohn ),( Oh Young Lee ),( Byung Chul Yoon ),( Ho Soon Choi ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Recently, gut microbiota has been received attention in pathogenesis of non-alcoholic fatty liver disease (NAFLD). The protective effects of Lactobacillus paracasei on NAFLD and possible mechanism were investigated. Methods: Forty male C57BL/6 mice were randomized into 4 groups for 12 weeks: control, NASH model (high fat+10% fructose diet), Lactobacillus paracasei and Lactobacillus plantarum groups. Hepatic fat deposition, inflammation, serum ALT, AST, and bilirubin were tested. Kupffer cell polarization was evaluated by flow cytometry using M1 and M2 marker. Intestinal permeability was measured by urinary Cr-EDTA amount. Results: Mean body weight of L. paracasei and L. plantarum group were lower than NASH model (38 g vs. 43g, p<0.05). Compared with NASH model mice, serum ALT and AST were significantly decreased in both L. paracasei group and L. plantarum group (ALT: 135.5 U/L vs. 92.9 and 53.7U/L, p<0.05). Intestinal permeability was decreased in both L. paracasei and L. planatrum group compared to NASH model (p<0.05).While hepatic fat deposition and lobular inflammation was significantly decreased in both L. paracasei and L. plantarum groups. Kupffer cell infiltration was significantly low in only L. paracasei group (p<0.05). M2 macrophage population increased in L. paracasei compared to NASH group (62.1% vs 50.2%, p<0.05). But Kupffer cell polarization of L. plantarum group did not showed differences compare to NASH group. Conclusions: Probiotics attenuate hepatic fat deposition and decrease ALT and AST levels in NASH model. L. paracasei, but not L. plantarum prevented steatohepatitis via modulation of Kupffer cell polarization.

Development of a piezoelectric unimorph using a mechanically pre-stressed substrate

Kang, Lae-Hyong,Lee, Jong-Won,Han, Jae-Hung,Chung, Sang-Joon,Ko, Han-Young Institute of Physics Publishing 2009 Smart materials & structures Vol.18 No.10

<P>This paper proposes a novel curved shape piezoelectric unimorph called PUMPS (piezoelectric unimorph with mechanically pre-stressed substrate) that uses a new and simple fabrication method. Generally, conventional pre-stressed curved shape unimorphs are made by using high-temperature adhesion processes and the thermal expansion coefficient mismatch of their constituent layers. In the conventional methods, therefore, it is necessary to use materials with different thermal expansion coefficients, high-temperature ovens or autoclaves, and special adhesives. In addition, the conventional methods generally require the re-poling of the piezoceramic actuator layer due to high-temperature adhesion near or above the Curie temperature of the piezoceramic. The present fabrication method uses mechanically pre-stressed substrates and a room temperature adhesion process instead of relying on a thermal coefficient mismatch. First, a substrate material is strained in the longitudinal direction and then a piezoelectric material is attached to the substrate. A difference of mechanical strains between the substrate and the piezoelectric layer causes the final manufactured actuator to curve. In this way, a new type of curved shape pre-stressed piezoelectric unimorph, PUMPS, can be easily fabricated at room temperature and can be used as a sensor, an actuator and an energy-harvesting element without the necessity of a re-poling process. A series of performance tests on the proposed PUMPS actuators was accomplished and the test results show that the actuation capability of PUMPS is comparable to that of conventional curved shape actuators, in spite of the much simpler manufacturing process. </P>

Antitumor Activity and Acquired Resistance Mechanism of Dovitinib (TKI258) in <i>RET</i>-Rearranged Lung Adenocarcinoma

Kang, Chan Woo,Jang, Kang Won,Sohn, Jinyoung,Kim, Sung-Moo,Pyo, Kyoung-Ho,Kim, Hwan,Yun, Mi Ran,Kang, Han Na,Kim, Hye Ryun,Lim, Sun Min,Moon, Yong Wha,Paik, Soonmyung,Kim, Dae Joon,Kim, Joo Hang,Cho, American Association for Cancer Research 2015 Molecular Cancer Therapeutics Vol.14 No.10

<P><I>RET</I> rearrangement is a newly identified oncogenic mutation in lung adenocarcinoma (LADC). Activity of dovitinib (TKI258), a potent inhibitor of FGFR, VEGFR, and PDGFR, in <I>RET</I>-rearranged LADC has not been reported. The aims of the study are to explore antitumor effects and mechanisms of acquired resistance of dovitinib in <I>RET</I>-rearranged LADC. Using structural modeling and <I>in vitro</I> analysis, we demonstrated that dovitinib induced cell-cycle arrest at G<SUB>0</SUB>–G<SUB>1</SUB> phase and apoptosis by selective inhibition of RET kinase activity and ERK1/2 signaling in <I>RET</I>-rearranged LC-2/ad cells. Strong antitumor effect of dovitinib was observed in an LC-2/ad tumor xenograft model. To identify the acquired resistance mechanisms to dovitinib, LC-2/ad cells were exposed to increasing concentrations of dovitinib to generate LC-2/ad DR cells. Gene-set enrichment analysis of gene expression and phosphor-kinase revealed that Src, a central gene in focal adhesion, was activated in LC-2/ad DR cells. Saracatinib, an src kinase inhibitor, suppressed ERK1/2 phosphorylation and growth of LC-2/ad DR cells. Taken together, these findings suggest that dovitinib can be a potential therapeutic option for <I>RET</I>-rearranged LADC, in which acquired resistance to dovitinib can be overcome by targeting Src. <I>Mol Cancer Ther; 14(10); 2238–48. ©2015 AACR</I>.</P>

Synthesis and Cytotoxicity of New Aromatic Ceramide Analogs with Alkylsulfonamido Chains

Kang, Joo-Sung,Kim, Seung-Yong,Choi, Su-Hang,Lim, Se-Jin,Im, Chae-Uk,Yim, Chul-Bu,Kim, Kyoung-Won 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

A series of D-erythro ceramide analogues, N-(2S,3R,4E)-1 ,3-dihydroxy-5-phenyl pent4-en-2-yl alkyl sulfonamides, were synthesized and evaluated for their in vitro cytotoxic activities against five human tumor ceil lines. The aromatic sullen amido ceramide analogue (10f)showed more potent cytotoxic activity than that of the B13, indicating that a sulfonamide group appears to serve as a bioisostere of an amide in drug design. Variations in the alkyl sulfonyl chain length signifcantly influenced the cytotoxic activity of the sulfonamido ceramide analogues, but the introduction of a para halogen on the phenyl ring of aromatic ceramide analogues had no affect on the activity.

Changes of Soil-Emission Gases and Microbial Diversity by Different Fertilizers Supplemented after Application of Livestock-Manure Compost in Greenhouse Soil

Hang-Won Kang(강항원),Jee-Yeon Ko(고지연),Hyang-Mee Park(박향미),Jae-Saeng Lee(이재생),Ui-Gum Kang(강위금),Kyeong-Bae Park(박경배) 한국토양비료학회 2000 한국토양비료학회지 Vol.33 No.1

Role of biofilm on the surface of membrane in intermittently aerated membrane bioreactors

Hang Sik Shin,Seok Tae Kang,Won Tae Lee 대한환경공학회 2002 대한환경공학회 학술발표논문집 Vol.2002 No.06

No-till Farming System: Research Direction and Outlook in Korea

Kang, Hang-Won,Kim, Min-Tae,Kim, Kwang Seop,Jeon, Weon-Tai,Ryu, Jin-Hee,Seong, Ki-Yeong 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.3

No-till farming system has been extensively studied all over the world as the effective method for maintaining the soil fertility. The general advantages of this system have been well known for reducing the labor, fuel, machinery, and irrigation cost as well as for increasing the soil quality through soil aggregation, water infiltration, microbial population and etc. Recently, it becomes more popular with the increase of interest on sustainable agriculture, especially because of its higher carbon sequestration potential compared to conventional tillage. Crop residue management should be essentially included to look forward to achieving the positive effect on reduction of greenhouse gas. Nonetheless, there are also negative opinions on effect of no-till farming system. For example, some researchers reported that soil physical properties were not improved by no-till under certain soil and climatic conditions. This means no-till farming systems were strongly affected by the soil characters and climatic conditions. Therefore, the researches to meet the specific-regional characters are greatly needed in order for no-till farming system to successfully settle in Korea. The objective of the review article is to present the future direction and perspective on no-till farming system in Korea. For this purpose, we summarized the results of domestic and foreign researches about no-till farming system until now. Specifically, the chapter on foreign research consisted of four parts: positive and negative effects, the effect in paddy soil, and latest research direction (2012-2013) of no-till farming systems. Whereas, review for domestic researches was divided into two main parts: paddy and upland soils. In the final chapter, the priorities for the optimum conservation tillage in Korea were discussed and proposed through the previous researches.

No-till Farming System: Research Direction and Outlook in Korea

Hang-Won Kang,Min-Tae Kim,Kwang Seop Kim,Weon-Tai Jeon,Jin-Hee Ryu,Ki-Yeong Seong 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.3

No-till farming system has been extensively studied all over the world as the effective method for maintaining the soil fertility. The general advantages of this system have been well known for reducing the labor, fuel, machinery, and irrigation cost as well as for increasing the soil quality through soil aggregation, water infiltration, microbial population and etc. Recently, it becomes more popular with the increase of interest on sustainable agriculture, especially because of its higher carbon sequestration potential compared to conventional tillage. Crop residue management should be essentially included to look forward to achieving the positive effect on reduction of greenhouse gas. Nonetheless, there are also negative opinions on effect of no-till farming system. For example, some researchers reported that soil physical properties were not improved by no-till under certain soil and climatic conditions. This means no-till farming systems were strongly affected by the soil characters and climatic conditions. Therefore, the researches to meet the specific-regional characters are greatly needed in order for no-till farming system to successfully settle in Korea. The objective of the review article is to present the future direction and perspective on no-till farming system in Korea. For this purpose, we summarized the results of domestic and foreign researches about no-till farming system until now. Specifically, the chapter on foreign research consisted of four parts: positive and negative effects, the effect in paddy soil, and latest research direction (2012-2013) of no-till farming systems. Whereas, review for domestic researches was divided into two main parts: paddy and upland soils. In the final chapter, the priorities for the optimum conservation tillage in Korea were discussed and proposed through the previous researches.