골뱅이(Buccinum Undatum) 알레르기 : 감작률과 다른 음식물 항원과의 연관성 Sensitization rate and its relationship with other food allergens

이재영,윤성호,서유진,최정희,서창희,남동호,김윤근,민경업,박해심 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1

Background : The common whelk (Buccinum undatum) is one of the largest and most common snails in the North Atlantic. In Korea and Japan, common whelk is a popular eatable shellfish. Although shellfish has been known as the one of the most common causes of food allergy, there has been no published report on allergenecity and clinical significance of this sea snail. In this study, we determined the sensitization rate to common whelk and its relationship with other food allergens in allergy patients. Method : We carried out the skin prick test (SPT) with commonly consumed food stuffs in Korea including common whelk in 1700 patients over 1 year. The specific IgE to common whelk were detected by enzyme-linked immunosorbent assay (ELISA). ELISA inhibition test using sensitized sera was conducted. Results : SPT to common whelk was positive (≥2+) in 83 (4.9%) patients studied. Twenty-four (38.7%) of 62 SPT-positive patients had high serum specific IgE to common whelk. ELISA inhibition test showed significant inhibitions by abalone as well as by common whelk and, minimal inhibition were noted by shrimp. Significant correlation was also noted in specific IgE levels between common whelk and abalone (r=0.58, F<.05). Conclusion : IgE-sensitization rate to common whelk was 4.9% in allergy patients. Further stuides are needed to evaluate the clinical significance of sensitized patients to common whelk, and to confirm a cross reactivity with abalone. (J Asthma Allergy Immunol 24 : 77-84, 2004)

淋菌의 藥劑耐性樣相에 對한 調査硏究

李承薰,石鍾聲,朴蕙瓊,金景俊,柳在根,李龍雨 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

고지혈증 환자에서의 인슐린 저항성과 내장지방비만과의 관계정상인과 관동맥 질환자에서 고지방 식이후 고중성 지방혈증의 비교

이혜진,신길자,박시훈,조홍근 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.7

광주시내 업종별 음식점의 주방기구와 관련된 주요 세균 및 이들의 계절별 변화

이홍열,유맹자,정해진,김근영,정희종 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

업종별 음식점에서 사용중인 주방기구 가운데 개숫물중에 존재하는 세균들을 분리·동정한 결과, 한색음식점에서 37균주, 일식음식점에서 23균주, 및 중식음식점에서 33균주가 분리되었고 이들 중 한식음식점에서 E. coli 등 16균주, 일식음식점에서 Listeria sp. 등 20균주, 중식음식점에서 Micrococcus sp. 등 15균주를 각각 동정하였다. 이들 세균들은 업종별 음식업소에서 주로 사용되는 색품재료에 존재하는 세균들과 밀접한 관계가 있는 것으로 밝혀졌고 E.coli와 같은 일부 세균들은 주방의 불결한 환경이나 종사자로부터 오염된 것으로 추정되었다. 업종별 음식점에서 사용중인 개숫물, 행주, 칼, 및 도마에 존재하는 세균의 분포는 일반세균수 및 대장균수가 모두 2월에서 8월까지 점점 증가하다가 11월에는 다시 감소하는 경향을 보였다. 특히 이같은 경향은 일반세균은 개숫물에서 대장균군은 칼에서 가장 뚜렷하게 나타났다. 따라서 식중독 발생 가능성이 가장 높은 여름철에는 칼, 도마 등 주방기구들은 정기적으로 열탕소독하는 철저한 위생처리가 필수적인 것으로 생각되었다. Thirty-seven, twenty-three, and thirty-three bacterial strains were isolated from dish-washing water which was selected as a kitchen supply commonly loaded the highest level of bacteria among various supplies using at Korean style, Japanese style, and Chinese style restaurants. Among these isolates, sixteen strains including Esherichia coli, twenty strains including Listeria sp., and fifteen strains including Micrococcus sp. were identified from Korean style, Japanese style, and Chinese style restaurants, respectively. This result suggested that most isolated bacteria were closely related to bacterial flora of raw food materials used in each style of restaurants and others could be contaminated from the surrounding environment and the employees. Total and coliform bacterial counts distributed in dish washing water, wiping cloths, knives, and cutting boards used at different types of restaurants were gradually increased from February to August and then decreased until November. This changing tendencies of total bacterial counts and coliform group counts were obvious in dish-washing water and in knives, respectively. Therefore, kitchen supplies such as wiping cloths, cutting boards, and knives should be treated carefully with hot water of above 45℃ at every certain period of time to kill some of these potential hazardous bacteria and to prevent outbreaking foodborne-illness by them, especially during Summer.

벼 멸구에 對한 Carbofuran의 殺蟲效果에 미치는 環境要因의 影響 (Ⅰ) 土性, 有機物, pH.溫度 : Effect of Environmental Factors on the Biological Toxicity of Carbofuran against the Brown Planthopper(Nilaparvata lugens STAL) Ⅰ.Soil Texture, Organic Matters, pH and Temperature

李炯來,李海根 충북대학교 농업과학기술연구소 1987 農業科學硏究 Vol.5 No.1

A study was conducted to determine the effects of environmental factors which were soil texture, organic matter, pH and temperature on the biological toxicity against the brown planthopper BPH, (Nilaparvata lugens STAL) when carbofuran 3% granule was applied in artificial controlled pots. The experimental results are shown as follows; 1.Soil texture did not affect the biological toxicity of carbofuran on the BPH until 7 days after treatment (DAT) of carbofuran 3%G, but thereafter loam type soil showed the higher efficacy than those of sandy loam and clay loam. 2.The lower contents of organic matters in soil, the higher mortality of the BPH. 3.Soil pH did not affect the mortality of the BPH until 4 DAT, but !he lower pH in soils, the higher mortality from 14 DAT. 4.When sprayed with BPMC, MIPC, carbaryl and diazinon, the effects of temperature on the mortality of the BPH showed that the higher temperature, the higher mortality at 1 DAT, but the reverse was the case at 4 DAT. In case of application with carbofuran and diazion granules, the mortality of carbofuran were decreased at 20℃ and that of diazinon at 20-25℃.

저선량 γ선 조사가 참박의 초기 생육과 효소 활성 및 광합성 능에 미치는 영향

이혜연,김재성,백명화,이영근,임돈순 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.3

저선량 γ선 조사가 참박의 초기생육과 생리활성에 미치는 효과를 알아보기 위하여 참박 종자에 γ선을 O~2OGy 수준으로 조사하여 생육을 관찰하였다. 저선량 γ선에 의한 참박의 초기생육은 대조구에 비해 4~16Gy에서 다소 증가하였고, 생육 조사시 측정한 자엽의 경우 catalase와 peroxidase활성이 대조구에 비해 8Gy조사구에서 가장 높았으며 본엽은 peroxidase 활성이 4Gy 조사구에서 확연히 증가하였다. 저선량 γ선을 조사한 박 식물체의 광 스트레스에 대한 반응효과는 광계Ⅱ의 광화학적 효율이 대조구와 8Gy의 경우 50%정도 감소되었으나 4Gy에서는 40%정도의 감소를 보였다. 반면 Fo는 광저해가 진행됨에 따라 약간의 증가를 보였으나 대조구나 저선량 조사구사이의 차이는 거의 없는 것으로 나타났다. 광계Ⅱ의 광양자 수율, φ_PSⅡ과 광계Ⅱ 반응중심의 흥분 포획능, 1/Fo-l/Fm 또한 광저해가 진행되는 동안 감소하여 φ_PSⅡ는 대조구와 8 Gy의 경우 20%정도 감소를 보인 반면 4Gy조사구는 15%의 감소를 보였고, 1/Fo-lF/m은 대조구와 8Gy는 55%,4 Gy는 45%의 감소를 나타냈다. 비광화학적 소멸인 NPQ는 대조구와 저선량 조사구 모두 70% 정도 감소되는 경향을 보였다. 이러한 결과를 볼 때 종자의 종피를 투과한 저선량의 γ선이 참박의 생육을 촉진시키고 4Gy에서 광 스트레스에 대한 저해가 감소되었다. Gourd seeds were irradiated with the doses of 0∼20 Gy to investigate the effect of the low dose ??-radiation on the early growth and physiological activity. The stimulating effects of the low dose ??-radiation on the early growth were not noticeably high, but were increased generally at 4∼16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with ??-radiation were increased at 8 Gy irradiation group. The peroxidase activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSⅡ, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Fm in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose ??-radiation. Changes in the effective quantum yield of PSⅡ, Φ_PSⅡ and 1/Fo-1/Fm, a measure of the rate constant of excitation trapping by the PSⅢ reaction center, showed similar pattern to Fv/Fm. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose ??-radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.

광주시내 업종별 음식점의 위생상태 및 식중독원인 세균의 분포 특성

이홍열,유맹자,정해진,김근영,정희종 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

업종별 음식점 주방의 위생상태는 일식>중식>한식의 순이었고 개인위생의 경우는 일식>한식>중식의 순이었는데 주방 및 종업원의 위생상태를 향상시키기 위해서는 음식점 규모의 대형화, 45℃ 이상의 온수공급, 주방시설의 표준화, 위생비닐장갑의 착용, 및 유니폼 착용이 신속하게 이루어져야 할 것이다. 업종별 대중음식점의 주방기구중의 세균분포는 전반적으로 일반세균수가 높게 나타났고 특히 대장균군수가 높게 나타났는데 음식물이 아니고 조리기구이긴 하지만 우리 음식점의 위생상 문제가 많은 것으로 생각된다. 미국 육군 Natick연구소가 제시한 음식물의 미생물 기준한계인 1 ×10??마리 이하, 대장균수가 100마리 이하와 비교할때 일반세균수의 경우는 아주 만족할만한 위생상태로 평가되었으나 대장균수는 개숫물의 경우 업종과 관계없이 높게 나타났고 행주와 칼의 경우 일식음식점에서만 높게 나타났으며 도마의 경우는 모두 100마리 이하로 양호한 위생상태로 평가되었다. 한식 전문음식점간의 세균수가 큰 차이를 보인 것은 규모와 운영방법이 서로 다르기 때문으로 앞으로 한식음식점에 대한 시설 및 환경개선에 대한 노력이 요구되었다. 또한 일식음식점간에는 일반세균수의 분포가 크게 다는 것으로 조사되었는데 특히 개숫물, 행주 및 도마중에 존재하는 미생물수가 크게 차이가 있었다. 등급별 한식음식점의 세균분포는 호텔급 음식점> 일반대중급 음식점> 여관급 음식점의 순으로 나타나 업소규모가 클수록 위생시설 및 위생습관의 수준이 높아 위생상태가 양호한 것으로 분석되었고 여관급음식점의 경우 위생상태가 낮은 것은 이용빈도가 낮은 것이 그 원인이 아닌가 생각되었다. Sanitary evaluation of kitchen area in different types of restaurants was the best at Japanese style restaurants and the worst aat Korean style restaurants. In personal hygiene Japanese style restaurant were evaluated as the best, but Chinese style restaurants were evaluated as the worst. Total and coliform bacterial counts were mostly showed high levels at all types of restaurants and especially this tendency was predominated in coliform counts. As compared to the standards suggested by U.S. Army Natick, however, total bacterial counts in all of supplies were evaluated as the satisfactory state. On the other hand, coliform counts in dish-washing water were much higher than the standard, which were not affected by the types of restaurant, and Japanese style restaurants were higher in wiping cloths and knives, but cutting boards were evaluated as satisfactory state with the counts less than 1.0 ×10².Bacterial counts in Korean style restaurants were greatly different depended on the size or type of restaurant. Total bacterial counts in Japanese style were also different among restaurants, especially in dish-washing water, wiping cloths, and cutting boards. According to the results of the evaluation of Korean style restaurant, bacterial distribution based on the class of restaurants, classified as hotel, motel, and public classes, hotel class restaurants showed lower levels of bacterial counts than public class. This result suggested that the larger and higher classes of restaurants maintained the satisfactory state without potential hazards.

유치 수복물에 따른 주변 법랑질의 탈회 저항성 : QLF를 이용한 연구

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, FiltekTM Z250(1군), F2000(2군), KetacTM N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(ΔQ)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ΔQ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ΔQ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 Δ(ΔQ)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다. The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with FiltekTM Z250(Group 1), F2000(Group 2), KetacTM N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(ΔQ) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ΔQ was increased as follows. : Group 1 (-110.79 ± 27.77) < Group 2 (-104.84 ± 28.95) < Group 3 (-90.16 ± 21.87) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ΔQ of all groups since 1st day of remineralization 3. The rate of remineralization, Δ(ΔQ)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.

고교 레슬링선수의 무산소성 파워 및 측정방법의 타당도에 관한 사례 연구

전해섭,윤재량,정동군,이희동,이정근 韓國體育大學校 1995 論文集 Vol.18 No.-

1) Anaerobic power by jump reach test record was not significant difference statistically between Free-style and Grecoroman-style player. 2) Anaerobic power by 50m Allout-running was not significant difference statistically between free-style player and Grecoroman-style player. 3) Anaerobic power by Lewis-nomogram After measure jump reach test record and weight was not significant difference statistically between Free-style player and Grecoroman-style player.. 4) Anaerobic maximal, Anaerobic mean power and total power were not significant difference statistically between Free-style and Grecoroman-style player. 5) Wingate test measurement method can substitution for Anaerobic power measurement method by Lewis-nomogram.

누에의 人工飼料 開發에 관한 硏究

孫海龍,李鎭根 慶北大學校 1990 論文集 Vol.49 No.-

This experiment was conducted to improve the quality of artificial diet for the silkworm., Bombyx mori. 1. All of the Corn, Barley, Rice were good for response, Corn was most recommendable starch source for artificial diet. 2. The precentage of under grown larvae could be reduced by the treatment of hexane when the soybean powder was used for starch source, which was especially effective on the diet for the 1st instar. 3. Feeding responses and growth at 4th instar larvae were varied by the individual moth, however the Japanese strains were superior to Chinese strains in general case. 4. All of the hybrid silkworm showed the better feeding response and growth than their original strains. 5. Both cocoon productivity and egg productivity of the original strains reared on the artificial diet were increased by the subsequent rearing through generation.