Sparse feature selection identifies H2A.Z as a novel, pattern-specific biomarker for asymmetrically self-renewing distributed stem cells

Huh, Y.H.,Noh, M.,Burden, F.R.,Chen, J.C.,Winkler, D.A.,Sherley, J.L. Elsevier 2015 Stem cell research Vol.14 No.2

There is a long-standing unmet clinical need for biomarkers with high specificity for distributed stem cells (DSCs) in tissues, or for use in diagnostic and therapeutic cell preparations (e.g., bone marrow). Although DSCs are essential for tissue maintenance and repair, accurate determination of their numbers for medical applications has been problematic. Previous searches for biomarkers expressed specifically in DSCs were hampered by difficulty obtaining pure DSCs and by the challenges in mining complex molecular expression data. To identify such useful and specific DSC biomarkers, we combined a novel sparse feature selection method with combinatorial molecular expression data focused on asymmetric self-renewal, a conspicuous property of DSCs. The analysis identified reduced expression of the histone H2A variant H2A.Z as a superior molecular discriminator for DSC asymmetric self-renewal. Subsequent molecular expression studies showed H2A.Z to be a novel ''pattern-specific biomarker'' for asymmetrically self-renewing cells, with sufficient specificity to count asymmetrically self-renewing DSCs in vitro and potentially in situ.

Parylene버퍼층 구조 H:LiNbO₃ 광변조기 제작

허현(H. Huh),문춘기(C. K. Moon),김민수(M. S. Kim),반재경(J. K. Pan),윤형도(H. D. Yoon),김성구(S. K. Kim),윤대원(D. W. Yoon) 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.4

뇌로 전이된 인세포암(Signet-Ring Cell Carcinoma) 1례 보고

허양옥,이동화,박응범,이유복,정헌화,김헌주,김상철,정상섭,이헌재 中央醫學社 1975 中央醫學 Vol.28 No.4

The metastatic carcinoma of the brain comprises about 15 to 25 per cent of total brain tumors and most of them originate from the lung. Additional primary sites such as the breast, kidney, large intestine, prostate are rare and the stomach as primary site is very rare. We experienced a case of metastatic signet-ring cell carcinoma to the brain originated from the stomach, which was thought as subdural hematoma at the time of admission.

多發性骨髓腫의 臨床的 觀察

허규열,고행일,김성연,고일향,김예회 인제대학교 1980 仁濟醫學 Vol.1 No.2

1977년 8월부터 1980년 7월까지 인제의대 부속 백 병원에서 13예의 자발성 골수종 환자를 관찰한 바 남녀의 비는 1.8 : 1이었으며 남자의 평균 연령은 51.4세, 여자의 평균 연령은 53.6세였다. 임상증상은 골동통이 77%에서 있었으며 류마치스성관절염, 퇴행성관절염, 신질환등으로 초진된 경우가 많았다. 혈액소견상 빈혈이 92%에서, 증가된 적혈구침강속도가 전체환자에서, 말초혈액소견상 형질세포화한 림파구가 31%에 있었다. 생화학검사는 총단백의 증가가 62%, 고칼슘혈증이 31%, 질소혈증이 69%, 고뇨산증이 89%, hypergammaglobulinemia가 38%에서 나타났다. 12예에서 전기영동을 시행한 결과 M-단백이 γ-region에 8예, β-region 3예, α2-region에 1예로 나타났다. 면역전기영동은 9예에서 시행결과 IgG 44%, IgA 22%, IgD 22%, light chain 11%였다. 방사선과적 검사는 85%에서 골용성병변이 있었으며 두개골 척추골 늑골 골반등의 순으로 있었다. 치료는 melphalan+prednisone이나 cyclophosphamide+prednisone을 사용하여 좋은 결과를 얻을 수 있었으며 melphalan 또는 cyclphosophamide 등의 alkylating agent와 prednisone 병용에 반응을 보이지 않은 환자에서는 제 2차적인 치료법으로 CCNU, Adriamycin 및 vincrstine을 이용한 병용요법을 시행하여 효과를 보았다. From August 1977 to July 1980, thirteen cases of multiple myeloma had been admitted to Paik Hospital, Inje Medical College. The ratio of male to female was 1.8 1 and the mean age was 51.4 years old in man and 53.6 years old in woman. The most common clinical findings were bone pain and tenderness. There were many cases which had been impressed as rheumatoid arthritis, degenerative arthritis, or renal problem at first medical examination. Hematologic findings were anemia in 92%, elevated E.S.R. in 100%, and plasmacytoid lymphocyte in 31%. Blood chemistry showed increased total protein in 62%, azotemia in 69%, hyperuricemia in 89%, hyperglobulinemia in 38%, and hypercalcemia in 31%. Serum protein electrophoresis in 12 cases revealed that M-spike was present in γ-region in 8 cases, in β-region in 3 cases and in α2-region in one case. Immunoelectrophoresis in 9 cases showed Ig G in 44%, Ig A in 22%, Ig D in 22% and light chain in 11%. Osteolytic lesions were noted in 85% and involed skull, spine and ribs in order. All 13 patients were treated with alkylating agent (melphalan or cyclophosphamide) and prednisone. Patients, who did not show response to the alkylating agent, were treated with CCNU, Adriamycin and vincristine in combination. Of 13 patients, six patients were lost to follow-up. One patient who had severe renal failure at the tinge of admission was dead during chemotherapy in conjuction with dialysis. Three patients survived 11, 13 and 32 moths each. The remaining three patients are still alive and well from 3+ months to 25+ months after initiation of chemotherapy.

Thickness-dependent solar power conversion efficiencies of catalytic graphene oxide films in dye-sensitized solar cells

Huh, S.H.,Choi, S.H.,Ju, H.M. Elsevier 2011 Current Applied Physics Vol.11 No.3

The catalytic effects of graphene oxide (GO) films were investigated for dye-sensitized solar cell (DSSC). The GO film consisted of a networked structure of two phases of GO with intercalated H<SUB>2</SUB>O and exfoliated GO. A maximum solar power conversion efficiency (η) of 2.30% was obtained for the ultrathin GO film. This η is higher than that of other reported types of DSSCs (0.84%) and organic solar cells (1.1%) with graphene electrodes. The catalytic effect of the GO film is attributed to the good ion permeability along the interlayer hydrophilic spaces; the electrons present in these spaces on the exfoliated GO were supplied to the iodide ions.

점안액 중의 benzalkonium ehloride에 의한 알레르기성 접촉 피부염 1례

허창훈,한광호,은희철 대한알레르기학회 2000 천식 및 알레르기 Vol.20 No.1

Benzalkonium chloride is one of the most commonly used preservatives in eyedrops, artificial tears, and solution for contact lenses, which has occasionally been reported as a causative agent producing allergic contact dermatitis. We have experienced a case of benzalkonium chloride induced allergic contact dermatitis in a 66-year-old woman presented with edema and erythematous plaque of the left eyelid and itching. She had been using two kinds of eyedrops for uveitis and cataract of her left eye for seven years, when dermatitis developed one month ago. Patch test with the European standard series, her own eyedrops, and their individual components gave positive reactions to neomycin sulfate, nickel sulfate, potassium dichromate, benzalkonium chloride. All of her eyedrops were prohibited and after being treated with topical prednisolone, her eruption disappeared completely.

핵연료 채널과 LIN 간격 측정을 위한 전자기 분포해석 및 원격장 와전류 신호 모델링

이동훈,정현규,정용무,허형,이윤상 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

Fuel channels including pressure tube(PT) and calandria tube(CT) are important components of pressurized heavy water reactor(PHWR). A sagging of fuel channel increases by heat and radiation exposure with the increasing operation time. The contact of fuel channel to liquid injection nozzle(LIN) is thus a critical issue in power plant safety. In order to solve this safety issue, the electromagnetic technique was applied to compliment the present inspection technology. Electromagnetic fields were investigated for the gap measurement between CT and LIN using FEM computer modeling. We calculated the electromagnetic fields, such as, magnetic flux density, current density near the fuel channel and checked the adaptability of RFEC technology. The RFEC Signals using the volume integral method(VIM) were simulated for obtaining the optimal inspection parameters, including frequency, inter-coil spacing, coil size and configuration. Finally, we development the remote field eddy current sensor that can CT/LIN gap measurement efficiently

한국 성인에서 비알콜성 지방간의 중증도에 따른 대사이상의 특징

김형진,김대중,김수경,김세화,이유미,안철우,차봉수,송영득,임승길,김경래,이현철,허갑범 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 30세 이상의 성인 한국인에서 지방간의 중증도와 대사증후군의 여러 요소들 (특히 인슐린저항성 및 중심성비만)과의 관련성을 분석하고자 하였다. 방법: 대상자는 2001년 4월부터 6월까지 건강검진센터를 방문한 사람들 중 만성 바이러스성 간염이나 일주일에 2회 이상 음주력이 있는 사람들을 제외한 1074명 (남자 502, 여자 572)에서 문진, 신체검사, 채혈 그리고 복부초음파를 시행하였다. 결과: 1074명중 522명에서 지방간이 양성이어서 양성율은 49%였으며 남성은 여성에 비해 높은 유병률을 보였다. (57 vs 42%, p<0.05). 당뇨병, 비만 그리고 이상지질증을 가지지 않은 사람들에서 비알콜성 지방간의 유병률은 20%였다. 지방간이 양성인 522명중 218명은 경도, 273명은 중등도, 31명은 중증의 지방간 소견을 보였다. 체질량지수, 허리둘레, 체지방률, 수축기혈압, aspartate aminotransferase, alanine aminotransferase, 총콜레스테롤, 중성지방, 그리고 총콜레스테롤/고밀도지단백 비는 지방간의 중증도에 따라 의미있게 증가하는 양상을 보였다(p<0.05). 다중회귀분석을 시행하였을 때 지방간의 중증도를 잘 반영하는 인자로는 허리둘레, alanine aminotransferase, HONA_IR, 총콜레스테롤/고밀도지단백비, aspartate aminotransferase, 그리고 수축기혈압의 순서로 나타났다. 공복혈당장애, 고혈압 및 인슐린저항성은 지방간을 가진 군에서 대조군보다 의미있게 높은 빈도를 보였을 뿐만 아니라 지방간의 중증도에 따라 차이를 보였다(p<0.05). 인슐린저항성은 심한 지방간에 비교위험도가 14.7(CI; 6.8∼32.0), 중등도의 지방간에서 6.9(CI; 4.6∼10.3), 경도의 지방간에서 5.7(CI; 3.6∼8.8)이었으며 HOMA_IR의 상위 1/4을 인슐린저항성이 있는 군으로 정의하였을 때 각군에 인슐리저항성을 가진 비율은 7.6, 32.0, 36.0, 그리고 55%였다(p<0.05). 지방간의 중증도에 따른 비알콜성 지방간염의 고위험군 비율은 각각 5.0, 21.6, 27.8, 그리고 58.1%였다(p<0.05). 결론: 우리나라의 비알콜성 지방간의 빈도는 서구인에 비해 높은 것으로 나타났다. 또한 비알콜성 지방간이 있을 경우 대사증후군과 관련된 여러인자들(특히 인슐린저항성과 중심성비만)과 비알콜성 지방간염의 위험이 현저히 증가하며 초음파의 중증도 분류에 따라 비례하였다. Background: We evaluated the frequency of non-alcoholic fatty liver disease, and the associations between the metabolic abnormalities and severity of non-alcoholic fatty liver disease in Korean adults using ultrasonography. Methods: We examined 1074 Korean adults above the age of 30 years, comprising of 502 men and 572 women, participating in medical check-ups at the Health Promotion Center. Hepatitis B and C serologies were negative, and the average weekly alcohol intake was ≤ 2 standard drinks. A standard interview, physical exam and biochemical study, were conducted, and an experienced operator carried out ultrasound liver studies. Results: 522 of the subjects had non-alcoholic fatty liver disease, and the remaining 552 did not. The frequency in the men was higher than that in the women (57 vs 42%, p<0.05). The frequency of non-alcoholic fatty liver disease in the subjects without diabetes, obesity or dyslipidemia was 20%. We classified subjects into 4 groups: the controls (n=552) and those with mild (n=218), moderate (n=273) or severe fatty liver disease (n=31), according to their ultrasonographic findings. BMI, waist circumference, body fat, systolic blood pressure, aspartate aminotransferase, alanine aminotransferase, total cholesterol, triglyceride, the total-to HDL-cholesterol ratio, impaired fasting glucose, hypertension and insulin resistance, were all significantly increase with the increased severity of non-alcoholic fatty liver disease (p<0.05). Following the multiple regression analyses, waist, alanine, aminotransferase, HOMAIR, the total- to HDL-cholesterol ratio, aspartate aminotransferase and systolic blood pressure, were all associated with the severity of non-alcoholic fatty liver disease. Odd ratios of insulin resistance in the mild, moderate and severe non-alcoholic fatty liver disease were 14.7 (CI: 6.8∼32.0), 6.9 (CI: 4.6∼10.3) and 5.7 (CI 3.6∼8.8), respectively. The percentages of subjects with insulin resistance in each group were 7.6, 32.0, 36.0 and 55.0% (p<0.05), respectively. The percentages of subjects with risk of non-alcoholic steatohepatits in each groups were 5.0, 21.6, 27.8 and 581% (p<0.05) respectively. Conclusion: The frequency of non-alcoholic fatty liver disease was relatively high in Korean adults. Proportional differences in metabolic abnormalities, relation to the severity of non-alcoholic fatty liver disease, were found by ultrasonography (J Kor Soc Endocrinol 17:514∼525, 2002).

극저온 시험장치의 제작과 AISI 300계열 스테인리스강의 극저온 인장변형거동

이해무,남승훈,허용학,이주진,방건웅 대한금속재료학회(대한금속학회) 1990 대한금속·재료학회지 Vol.28 No.11

For practical application of cryogenic engineering, development and characterization of structural materials for use at low temperatures are essential. For these purposes, a system for mechanical testing at liquid helium temperature was developed and it was shown that the precision and accuracy of the system met the requirements of standards for materials testing machines. Using this system, tensile deformation behavior of AISI 304, 316 and 310S austenitic stainless steels at cryogenic temperatures was investigated. Tests were conducted on round, tensile specimens having a 6.25㎜ diameter at 4, 77, and 295 K and loading rate was 0.5㎜/min. Serrations were observed in all alloys at 4 K. The stress-displacement curves at 77 and 4 K showed different tendency from those at 298 K. As the testing temperature decreased, ultimate strengths of 304 and 316 were largely increased compared to the increase of yield strengths, but the increase of ultimate strength of 310S was almost the same to that of yield strength. Type 310S had the highest yield strength and the lowest tensile strength at all temperatures. These tensile characteristics were considered to be strongly affected by austenite stability.

교차 배열된 지르코늄관 간격 측정을 위한 전압 평면에서의 신호해석

정현규,이윤상,이동훈,허형,정용무 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

The eddy current(EC) signals were evaluated for gap measurement of cross aligned zirconium tubes for preventing the contact which might be the safety concern. Liquid lnjection Nozzle(LIN) tube and Calandria tube(CT) were cross-aligned horizontally and LIN could be detected by inserting the EC probe into pressure tube (PT). Signal analysis on the voltage plane was applied to describe the position and shape of possible defects using several frequencies. Test variables are lift-off, PT thickness variation, and gap variation between PT and CT All the EC measurements in the laboratory were done in variance with the gap spacings and showed the possibility to evaluate the gap between CT. and LIN tube by analyzing the signal on voltage plane. Electromagnetic technique could be a potential tool for inspecting the inter-tube gap in CANDU reactor.