Suppression of Lipopolysaccharide-Induced Microglial Activation by a Benzothiazole Derivative

Kim, Eun-A,Kim, Han-Wook,Ahn, Jee-Yin,Hahn, Hoh-Gyu,Kim, Key-Sun,Kim, Tae-Ue,Cho, Sung-Woo Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.1

We previously reported that KHG21834, a benzothiazole derivative, attenuates the beta-amyloid (A${\beta}$)-induced degeneration of both cortical and mesencephalic neurons in vitro. Central nervous system inflammation mediated by activated microglia is a key event in the development of neurodegenerative disease. In this study, we show that KHG21834 suppresses inflammation-mediated cytokine upregulation. Specifically, KHG21834 induces significant reductions in the lipopolysaccharide-induced activation of microglia and production of proinflammatory mediators such as tumor necrosis factor-${\alpha}$, interlukin-1${\beta}$, nitric oxide, and inducible nitric oxide synthase. In addition, KHG21834 blocks the expression of mitogen-activated protein kinases, including ERK, p38 MAPK, JNK, and Akt. In vivo intracerebroventricular infusion of KHG21834 also leads to decreases the level of interleukin-1${\beta}$ and tumor necrosis factor-${\alpha}$ in brain. These results, in combination with our previous findings on A${\beta}$-induced degeneration, support the potential therapeutic efficacy of KHG21834 for the treatment of neurodegenerative disorders via the targeting of key glial activation pathways.

Estrogen Receptor-${\alpha}$ 유전자 5' 영역의 Single Nucleotide Polymorphism의 탐색과 한우와 Holstein에서 번식능력 및 산유능력과의 관계

염규태,전향아,박해금,김영신,김현,김재환,성환후,조영무,조재현,고응규,Yeom, Gyu-Tae,Jeon, Hyang-A,Park, Hae-Geum,Kim, Young Sin,Kim, Hyun,Kim, Jae Hwan,Seong, Hwan-Hoo,Cho, Young Moo,Cho, Jae-Hyeon,Ko, Yeoung-Gyu 한국동물번식학회 2014 Reproductive & developmental biology Vol.38 No.3

This study was conducted for SNPs in the 5'-regions of estrogen receptor-${\alpha}$ (ESR-${\alpha}$), and association with calving interval (CI), service per conception (SPC) and 305 days milk yield in Hanwoo and Holstein dairy cattle. The genetic improvement was incurred low reproduction performance. The objective of this study was to investigate connections between single nucleotide polymorphisms (SNP) of Estrogen receptor-${\alpha}$ (ESR-${\alpha}$) with reproduction performance (calving interval, service per conception, and 305 d milk yield) in Hanwoo and Holstein dairy cattle. Hanwoo and Holstein blood samples were collected from 183 and 124 dam of breeding farms and DNA was extracted. Primer design was based on NCBI GenBank (Accession No. AY340579). The PCR-RFLP method with Bgl I was used to genotype the cattle. The result showed two variants of the ESR-${\alpha}$ gene. The Bgl I cut the 492 bp amplification product into 322 bp and 170 bp fragments for allele G, while allele A remained uncut, resulting in two restriction fragments for homozygote G/G and three fragments for heterozygote A/G. We found two of different genotypes in these breeds, A/G and G/G. In Hanwoo, the A/G genotype frequency was 0.13, and G/G was 0.87. The CI of A/G was $382.18{\pm}10.03$ days, and G/G was $381.69{\pm}5.22$ days. The SPC of A/G was $1.62{\pm}0.16$, and G/G was $1.32{\pm}0.04$. While CI showed no significance difference, SPC exhibited significant difference (p<0.05). In Holstein cattle, the frequency of genotype A/G was 0.02 and G/G was 0.98. The 305 days milk yield of A/G was $7,253.00{\pm}936.00kg$ and of G/G was $8,747.51{\pm}204.88kg$, showing no significant difference.

Design and Synthesis of A₃ Adenosine Receptor Ligands, 2´-Fluoro Analogues of Cl-IB-MECA

Kim, Hea Ok,Park, Jae Gyu,Moon, Hyung Ryong,Gunaga, Prashantha,Lim, Moo Hong,Chun, Moon Woo,Jacobson, Kenneth A.,Kim, Hee-Doo,Jeong, Lak Shin 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

Synthesis of 2'-deoxy-2'-fluoro-N^(6) -substituted adenosines as bioisosteres of Cl-IB-MECA and their binding affinities to A₃ adenosine receptor are described.

Intestinal Nematodes from Small Mammals Captured near the Demilitarized Zone, Gyeonggi Province, Republic of Korea

Deok-Gyu Kim,Jae-Hwan Park,Jae-Lip Kim,Bong-Kwang Jung,Sarah Jiyoun Jeon,Hyemi Lim,Mi Youn Lee,Eun-Hee Shin,Terry A. Klein,Heung-Chul Kim,Sung-Tae Chong,Jin-Won Song,Luck-Ju Baek,Jong-Yil Chai 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

A total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including Apodemus agrarius (n = 1,400), Microtus fortis (167), Crocidura lasiura (91), Mus musculus (32), Myodes (= Eothenomys) regulus (9), Micromys minutus (6), and Tscherskia (= Cricetulus) triton (3), were live-trapped at US/Republic of Korea (ROK) military training sites near the demilitarized zone (DMZ) of Paju, Pocheon, and Yeoncheon, Gyeonggi Province from December 2004 to December 2009. Small mammals were examined for their intestinal nematodes by necropsy. A total of 1,617 rodents (100%) and 91 (100%) soricomorphs were infected with at least 1 nematode species, including Nippostrongylus brasiliensis, Heligmosomoides polygyrus, Syphacia obvelata, Heterakis spumosa, Protospirura muris, Capillaria spp., Trichuris muris, Rictularia affinis, and an unidentified species. N. brasiliensis was the most common species infecting small mammals (1,060; 62.1%) followed by H. polygyrus (617; 36.1%), S. obvelata (370; 21.7%), H. spumosa (314; 18.4%), P. muris (123; 7.2%), and Capillaria spp. (59; 3.5%). Low infection rates (0.1-0.8%) were observed for T. muris, R. affinis, and an unidentified species. The number of recovered worms was highest for N. brasiliensis (21,623 worms; mean 20.4 worms/infected specimen) followed by S. obvelata (9,235; 25.0 worms), H. polygyrus (4,122; 6.7 worms), and H. spumosa (1,160; 3.7 worms). A. agrarius demonstrated the highest prevalence for N. brasiliensis (70.9%), followed by M. minutus (50.0%), T. triton (33.3%), M. fortis (28.1%), M. musculus (15.6%), C. lasiura (13.2%), and M. regulus (0%). This is the first report of nematode infections in small mammals captured near the DMZ in ROK.

Attenuation of β-amyloid-induced neuroinflammation by KHG21834 in vivo

( Eun A Kim ),( Hoh Gyu Hahn ),( Tae Ue Kim ),( Soo Young Choi ),( Sung Woo Cho ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

Beta-Amyloid (Aβ)-induced neuroinflammation is one of the key events in the development of neurodegenerative disease. We previously reported that KHG21834, a benzothiazole derivative, attenuates Aβ-induced degeneration of cortical and mesencephalic neurons in vitro. In the present work, we show that KHG21834 reduces Aβ-mediated neuroinflammation in brain. In vivo intracerebroventricular infusion of KHG21834 leads to decreases in the numbers of activated astrocytes and microglia and level of proinflammatory cytokines such as interleukin-1β and tumor necrosis factor-α induced by Aβ in the hippocampus. This suppression of neuroinflammation is associated with decreased neuron loss, restoration of synaptic dysfunction biomarkers in the hippocampus to control level, and diminished amyloid deposition. These results may suggest the potential therapeutic efficacy of KHG21834 for the treatment of Aβ-mediated neuroinflammation. [BMB reports 2010; 43(6): 413-418]

선진기업복지제도 도입지원사업의 경제적 효과성 분석

권진아(Jin-A Kwon),안영규(Young-Gyu Ahn),김현수(Hyun-Soo Kim),박경일(Kyung-Il Park) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.10

본 연구는 자료포락분석(DEA : Data Envelopment Analysis)을 이용하여 선진기업복지제도 실행에 따른 경제적 효과성을 분석하여, 중소기업들이 선진기업복지제도를 적극 도입 시행하도록 기여하는데 그 목적이 있다. 이를 위해, 설문회수 자료 중 투입-산출 지표와 관련된 3가지 유형의 - A유형(기본+심화, 도입)기업 36개, B유형(기본+심화, 미도입)기업 5개, C유형(기본, 미도입)기업 7개 - 총 48개 기업을 표본대상으로 하여 선진기업복지제도 도입과 관련된 경제적 효과성을 측정해 보았다. 분석 결과, 컨설팅을 받은 후 선진기업복지제도 중 어떤 하나의 제도라도 도입한 기업의 경제적 효과성이 컨설팅을 받은 후 관련 제도를 도입하지 않은 기업의 경제적 효과성보다 대체로 높게 나타나, 근로복지공단에서 실시하는 컨설팅을 통해 제도를 도입할 경우 긍정적인 경제적인 효과가 있음을 확인할 수 있었다. 이러한 연구결과를 통해 근로복지공단에서는 컨설팅을 보다 적극적으로 실시할 필요가 있으며 컨설팅만 받고 관련 제도 도입을 망설이는 기업을 찾아내고 그 이유에 대하여 구체적으로 파악해 볼 필요가 있음을 시사하고 있다. The purpose of this study is to analyze the economic effectiveness of advanced enterprise welfare system utilizing DEA(Data Envelopment Analysis) and contribute to the adoption and implementation of the system conducted by Korea Workers’ Compensation & Welfare Service(COMWEL). We classified 48 sample data into 3 categories : A category(basic consulting & intensive consulting, adoption) is 36, B category(basic consulting & intensive consulting, non-adoption) is 5, and C category(only basic consulting, adoption) is 7. A consulting fee is used as input variable, earning per employee and average employee tenure are used as output variables. As a result from DEA, we find out the fact that the economic effectiveness of A category is better than the economic effectiveness of B and C category and it comes to the conclusion that the consulting service provided by COMWEL has a positive effect on the adoption and implementation of advanced enterprise welfare system. Therefore, COMWEL is required to perform consulting service to small & medium business more actively and is needed to look at the reason why some businesses hesitate to accept the relevant system.

Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage

Lee, Kyoung-Ja,Kim, Bok-Ran,Cho, Young-A,Song, Yun-Gyu,Song, Jae-Young,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Cho, Myung-Je,Rhee, Kwang-Ho,Seo, Ji-Hyun,Youn, Hee-Shang,Lee, Woo-Kon 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4

The mouse model is alleged to be a useful tool for understanding of pathophysiological roles of Helicobacter pylori in the development of gastric disorders. However, it has been observed that H. pylori strains significantly differed in their fitness in mice and even mouse strains differed in their susceptibilities to a H. pylori strain. Bacterial components of H. pylori which could affect on its fitness in mice have to be elucidated for the establishment of the mouse model for H. pylori infections. In the comparison of colonization ability between two H. pylori Korean isolates, 51 (isolated from a patient with duodenal ulcer) and 52 (isolated from a patient with gastric cancer), 52 could colonize better than 51 on the gastric mucosa of mouse. Proteome components of H. pylori 52, as a good colonizer and H. pylori 51, as a poor one were quantitatively compared each other. Five bacterial proteins including catalase, urease subunit alpha/beta, enolase and ferritin, were up-regulated in 52. In addition, the respective proteome components of the two strains were also compared with their mouse-passaged homologous strains. Seven and five proteins, which included catalase, flagellin A/B in common, were up-regulated in mouse-adapted 51 and 52, respectively. Among the fourteen identified proteins, urease subunit alpha/beta, flagellin A/B, catalase, ferritin, superoxide dismutase and neutrophil-activation protein have been previously known to be necessary to gastric colonization of H. pylori in animal models. The other up-regulated proteins including enolase, elongation factor Tu and fructose-bisphosphate aldolase have been reported to be associated with acid tolerance of H. pylori. These data provide confirmatory evidence for the importance of those proteins in the development of H. pylori-associated gastric disorders.

Spinach Saponin-Enriched Fraction Inhibits Platelet Aggregation in cAMP- and cGMP-Dependent Manner by Decreasing TXA2 Production and Blood Coagulation

( Hyun Jeong Cho ),( Sun A Choi ),( Chun Gyu Kim ),( Tae Sung Jung ),( Jeong Hwa Hong ),( Man Hee Rhee ),( Hye Jin Park ),( Hwa Jin Park ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.2

In this study, we investigated the effect of spinach saponin-enriched fraction (SSEF) on collagen (10 μg/ml)-stimulated platelet aggregation. SSEF inhibited collagen-induced platelet aggregation, and which was involved in the inhibition of thromboxane A2 (TXA2) production, an intracellular Ca2+ -agonist as an aggregation-inducing autacoidal molecule. In addition, SSEF significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), intracellular Ca2+ -antagonists as aggregation-inhibiting molecules, in collagen-stimulated platelets. These results suggest that SSEF might inhibit Ca2+-elevation and TXA2 formation by increasing the production of Ca2+-antagonistic molecules cAMP and cGMP. These mean that SSEF is a potent inhibitor of collagen-stimulated platelet aggregation. On the other hand, prothrombin time (PT) and activated partial thromboplastin time (APTT) were potently prolonged by SSEF. These findings suggest that SSEF prolongs the internal time between the conversion of fibrinogen to fibrin. Accordingly, our data demonstrate that SSEF may be a crucial tool for a negative regulator during platelet activation and blood coagulation on thrombotic diseases.

원료 배합 및 소금 농도에 따른 대맥장의 품질 특성

강혜정 ( Hye Jeong Kang ),유진아 ( Jin A Yoo ),박정미 ( Jung Mi Park ),김상희 ( Sang Hee Kim ),송인규 ( In Gyu Song ),최혜선 ( Hye Sun Choi ),윤향식 ( Hyang Sik Yoon ) 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.2

검은콩 속성장인 대맥장의 실용화를 위해 검은콩과 보리 함량을 달리하여 메주를 만든 후, 소금 농도와 수분 첨가량을 다르게 하여 장을 담근 후 발효기간 중 품질 특성을 분석하였다. 대맥장이 발효되면서 pH는 점차 감소하였고, 총산은 증가하였다. 아미노태 질소 함량은 발효 중 현저하게 증가하였으며, 15% 염도에서 보다 10% 염도 조건에서 아미노태 질소 함량이 높은 것으로 나타났다. 대맥장 발효 70일째, 일반세균수는 발효기간에 따른 세균수의 차이를 보이지 않았으며, 유산균의 경우 10% 염도에서 발효기간이 증가함에 따라 2~3 log cycle 정도 감소하였고, 처리구 A와 15% 염도인 처리구 D에서는 유산균이 검출되지 않았다. 총 폴리페놀 함량은 발효 중 증가하였으며, 10% 염도 조건에서 15% 염도 조건에서 보다 높은 값을 나타내었다. DPPH 라디칼 소거 활성능은 발효기간에 따라 증가하였으며, 발효 70일째에는 처리구 간 차이가 없는 것으로 확인되었다. 발효 70일된 속성장의 관능평가를 실시한 결과, 전반적인 기호도는 검은콩과 보리를 5:5 또는 7:3의 비율로 메주를 만든 후, 10% 염도에서 메주의 1.5배의 수분을 첨가하여 제조한 B와 C처리구가 우수한 것으로 나타났다. This study investigated the quality characteristics of black soybean paste(Daemacjang) with black soybean content and salt concentration. The total acidity increased as the pH decreased during fermentation period. The amino-type nitrogen content of all samples increased significantly during fermentation time. Total cell counts was not significantly different during fermentation, and mold counts decreased at 3 log cycles. The cell counts of lactic acid bacteria decreased approximately 2 to 3 log cycles in 10% salt concentration (treat B and C), and were not detected in treat A and D. Total polyphenol contents of treat A, B, and C were 42.77%, 52.99% and 52.95% during fermentation up to 70days, respectively, which was higher than D (39.86%). In a sensory evaluation, treat B showed the highest scores for overall acceptability.

경수손상 환자에서 연령에 따른 재활 치료의 효과

임재영,신형익,김규리,김동아,김지영,나인수,이은선 대한노인병학회 2002 대한노인병학회지 Vol.6 No.3